[Development of a carbamazepine determination method based on high-performance liquid chromatography with diode array]. / Razrabotka metodiki opredeleniya karbamazepina na osnove vysokoeffektivnoi zhidkostnoi khromatografii s diodno-matrichnym detektirovaniem.

Carbamazepine is used in the treatment of convulsive disorders, trigeminal neuralgia and mental illnesses such as bipolar disorder and pain syndromes. It has been found in clinical applications that carbamazepine causes many serious adverse drug reactions, which are associated with drug concentration in blood and dosage in clinical use. Due to its pharmacological properties, carbamazepine can be life-threatening in an overdosage, making the therapeutic drug control of carbamazepine important in any clinical settings. Creation of a method for carbamazepine quantification in biological objects during forensic chemical examinations is an urgent problem. A method of high-performance liquid chromatography with diode array detection is proposed for carbamazepine quantitation. The stability of proposed conditions for carbamazepine analysis has been proved in the modifying study conditions (flow rate of eluent, its composition, pH of buffer, column oven temperature). The relative standard deviation of retention time and areas of chromatographic peaks of carbamazepine did not exceed 0.5%.

[The choice of the conditions for the preparation of the pregabalin samples from the biological fluids with the use of the liquid-liquid and solid phase extraction techniques]. / Vybor uslovii probopodgotovki pregabalina iz biologicheskikh zhidkostei metodami zhidkost'-zhidkostnoi i tverdofaznoi ékstraktsii.

The objective of the present study was the development of the methods for the isolation and identification of pregabalin for the purpose of chemical toxicological analysis (CTA). The authors describe the methods used to obtain pregabalin from the model mixtures of urine and blood plasma with the use of liquid-liquid and solid phase extraction. In addition, a method for the quantitative determination of pregabalin by means of combined gas chromatography mass-spectrometry was applied.

[Optimization of the conditions for the detection of psychoactive compounds of the cholinoblocker and antidepressant groups with the use of the microcolumn HPLC technique].

The objective of the present study was to evaluate the possibility of using the BD-2003 database to separate the mixtures of various antidepressants by high-performance liquid chromatography (HPLC) based on the MiLiChrome A-02 complex with an EcoNova UV detector. The parameters of identification are described. The possibility of modification of the standard conditions for the analysis is demonstrated as exemplified by the identification of certain cholinoblockers. The proposed approach to the separation of psychoactive preparations provides a basis for the development of methods for the determination of these substances in biological tissues and fluids.