The Role of Knowledge on Nursing Students' Attitudes toward Organ Donation: A Cross-Sectional Study.

BACKGROUND: Nursing students constitute the future nursing workforce; their knowledge can readily influence potential donors' decisions on organ donation. This study aimed to assess nursing students' knowledge of organ donation, determine its impact on their attitude, and identify relevant factors. METHODS: A cross-sectional comparative study assessed the level of knowledge and attitude toward organ donation among nursing students using the following two questionnaires: (1) knowledge about organ donation and (2) attitudes toward posthumous organ donation. Non-probability convenience sampling was employed to collect data. RESULTS: A total of 278 nursing students demonstrated a low level of knowledge on organ donation, with a score of 6.43 out of 15. This influenced their attitude toward organ donation (χ2 (3) = 33.91, p < 0.001). Nursing students who were willing to donate their organs showed higher knowledge (7.33 ± 3.23) compared to those who were not willing to donate their organs (5.21 ± 3.09), p < 0.001. Registered donors had higher knowledge (8.52 ± 2.99) than non-donors (5.80 ± 3.17), p < 0.001. CONCLUSIONS: Even though nursing students typically favor organ donation, findings have revealed a low knowledge score. Therefore, it is necessary to improve knowledge of organ donation through nursing curricula and research, which could potentially increase the number of donors among future nursing students and, by extension, the broader population.

Can action tendencies be counteracted by inducing incompatible emotions? Considering instances of anxiety and anger.

BACKGROUND: The incompatible response hypothesis suggests that emotions and other affective states can counteract each other when incompatible. With the present study, we focused on two negative emotions (anger and anxiety) associated with different action tendencies (approach vs. avoidance). Specifically, we wanted to investigate if an anxiety manipulation, subsequent to an anger manipulation, would show a counteracting effect of the approach action tendencies associated with the initial anger manipulation and vice versa for anxiety and avoidance tendencies. METHODS: We conducted a preregistered online experiment (N = 173). We evaluated changes from when the individual (1) was presented with a task in relation to a specific goal (e.g., anxiety induction: recordings of students' view on climate changes), (2) received a subsequent emotion induction framed within an unrelated task and goal (e.g., anger induction: student feedback on changes to the economic student compensation system), (3) after which they were asked to return to the initial task (e.g., from the anger induction back to the anxiety induction). Primary outcomes included visual and verbal measures of action tendencies, and secondary outcomes included appraisals and emotion experience. RESULTS: The results showed no evidence of a counteractive effect by inducing emotions unrelated to the initial task and with incompatible action tendencies. Rather, results pointed to spill-over effects, which should be seen in light of the anger conditions resulting not only in increase anger and irritability but also anxiety and nervousness. CONCLUSIONS: The lack of counteractive effects could be due to either the mixed emotions induced by the anger condition or the compatibility of motivational context (i.e., threat) of anxiety and anger. Future research needs to refine the incompatible response hypothesis, honing the ways in which incompatibility is needed for emotion alteration, for instance by investigating the role of the motivational context.

The Relationship between Happiness and Mental Health among Saudi Women.

The happiness and mental health of individuals are crucial for national developments. In Saudi Arabia, wellbeing occupies a central position in Vision 2030, along with women's empowerment. Rapidly changing rights and responsibilities might result in more sources of stress. The aim of this study was to explore happiness and mental health among Saudi women during their contributions to a fast-growing nation in all fields. We assessed happiness and mental health (somatic symptoms, depression, anxiety, insomnia, and social dysfunction) in 308 Saudi women aged 15-50 years using a self-administered online survey comprising the short Oxford Happiness Questionnaire (OHQ-8) and the General Health Questionnaire (GHQ-28). The participants demonstrated general satisfaction yet showed rising psychological distress. Married women reported better mental health compared to single women, particularly with regards to anxiety, insomnia, and depression. Depression was highest among younger women. Employed women demonstrated lower social functioning compared to unemployed women. Women with a higher educational level showed lower social functioning. Happiness scores were significantly and inversely related with overall mental health scores as well as mental health subscales (somatic, anxiety, and depression), except for social dysfunction, which showed a positive correlation to happiness scores. This study contributes to the body of literature on women's mental health and happiness by providing recommendations for improving both as well as directions for future research.

Immunohistochemical expression of estrogen receptor alpha in the maxillary sinus, pulp, and periodontal ligament of adjacent teeth in late pregnancy in rats.

This study aimed to assess the histological changes in the maxillary sinus and its adjacent dental tissues as pulp and periodontal ligament during pregnancy and investigate the role of estrogen hormone in these changes through the detection of estrogen receptors in these tissues. Sixteen adult female rats were used and were allocated into two groups: control non-pregnant (n = 8) and pregnant (n = 8). They were sacrificed and their heads were prepared for histological and immunohistochemical examination for estrogen receptor alpha. Our results revealed that pregnant rats revealed inflammatory changes in the sinus as thick epithelial lining, loss of cilia, swollen goblet cells, intraepithelial and interstitial edema. The lamina propria demonstrated considerable infiltration of inflammatory cells, glandular hyperplasia with vacuolar degeneration, and vascular congestion. Periodontal ligament and pulp revealed hyperemia and vascular congestion. Immunohistochemical examination of estrogen receptor alpha in the maxillary sinus and adjacent dental tissues (Periodontal ligament and pulp) in pregnant rats revealed a significant increase in its expression in all examined tissues. In conclusion, there was an increase in expression of ERα in the sinus mucosa and dental tissues during pregnancy together with slight inflammatory changes in these tissues. Hence, dentists should be aware of the effect of these changes on the pregnant women avoiding teeth extraction due to misdiagnosis of dental, periodontal or sinus pain after exclusion of true pathologies.

Using bodily displays to facilitating approach action outcomes within the context of a personally relevant task.

BACKGROUND: Considerable attention has been paid to the effect of bodily (expansive and contractive) displays on affective, behavioral, and hormonal outcomes. However, the majority of past studies are limited by a lack of control groups with neutral displays and low personal relevance of the experimental tasks employed. The present study aimed to investigate the effect of adopting different bodily displays, including neutral displays, within the context of a personally relevant task. METHODS: In an experiment with healthy participants (N = 90), we investigated the effects of two different bodily manipulations (i.e., expansive and contractive), compared to a control group (i.e., neutral displays). Effects were evaluated in terms of completed valued action in addition to processes considered potentially helpful in preparing and motivating the individual to take valued action, including a change in emotion experience, action tendencies, and appraisals. RESULTS: Several main effects were detected and only few significant interaction effects were revealed. In case of group differences, results showed that expansive bodily displays outperformed the control group, leading to more positive emotions, more approach action tendencies, less negative emotion variability, and less avoidance action tendencies toward threat. DISCUSSION: These results mainly suggest that identifying a valued action and explicating the underlying motivational conflict may be beneficial regardless of bodily displays. This conclusion runs somewhat counter both to our hypotheses and to findings in recent meta-analytic work. However, previous experiments have not evaluated the effect of bodily displays within a personally relevant context.

Lived Experiences of Female Nurses with COVID-19 Deaths on Their Watch.

The COVID-19 pandemic has had a tremendous effect on health systems, leading to a spike in stress, anxiety, and depression conditions among healthcare workers worldwide. Considering the mental health status of nurses, a pillar of the health system, is crucial for assuring the quality of the healthcare provided during and after pandemics. This study aimed to explore the experiences of female nurses who witnessed their patients dying of COVID-19. Seven female COVID-19 nurses were interviewed in person. Thematic analysis of the respondents' verbatim answers was implemented. Six main themes were identified. Theme 1 illustrates nurses' experience of emotional and psychological trauma as they witnessed their patients with COVID-19 die under their care. Theme 2 reveals aftershock reactions, including somatization, isolation, and emotional disengagement. Theme 3 highlights the hindrances and difficult situations related to the in-hospital care of COVID-19 patients. Theme 4 demonstrates victorious feelings and celebration of the patient's survival. Theme 5 emphasizes the nurse's ability to be calm and recognize the takeaways or lessons they have learned from this phase for their careers and lives. Theme 6 sheds light on nurses' emerging abilities and competencies. This study will hopefully provide a basis for a debriefing program that might be beneficial to the nurses and the health system. This might affect nurses' ability to work closely with patients, cope emotionally with challenges, and fulfil their professional tasks.

Activation of the human insulin receptor by non-insulin-related peptides.

The human insulin receptor signalling system plays a critical role in glucose homeostasis. Insulin binding brings about extensive conformational change in the receptor extracellular region that in turn effects trans-activation of the intracellular tyrosine kinase domains and downstream signalling. Of particular therapeutic interest is whether insulin receptor signalling can be replicated by molecules other than insulin. Here, we present single-particle cryoEM structures that show how a 33-mer polypeptide unrelated to insulin can cross-link two sites on the receptor surface and direct the receptor into a signalling-active conformation. The 33-mer polypeptide engages the receptor by two helical binding motifs that are each potentially mimicable by small molecules. The resultant conformation of the receptor is distinct from-but related to-those in extant three-dimensional structures of the insulin-complexed receptor. Our findings thus illuminate unexplored pathways for controlling the signalling of the insulin receptor as well as opportunities for development of insulin mimetics.

Motor alterations in depression and anxiety disorders: A systematic review and meta-analysis.

BACKGROUND: Psychomotor retardation has long been recognized as a major feature of depression, and anxiety disorders have been linked with freeze and flight motor responses. This systematic review and meta-analysis aimed a) to synthesize available evidence of motor alterations comparing individuals with depression and anxiety disorders to healthy individuals and b) to evaluate the effect of experimental manipulations of motor displays within these clinical groups. METHOD: The databases PubMed and PsycINFO were searched for studies either assessing motor differences between clinical and healthy control groups or manipulating the motor system within a clinical group. RESULTS: The literature search yielded 87 relevant papers, comprising 82 studies comparing a clinical group to a healthy group and 5 studies investigating motor manipulations within a clinical sample. The results of the meta-analysis (K = 71) indicated a statistically significant combined estimate of differences between healthy and clinical groups (g = 0.38 [0.31, 0.45], adjusted for publication bias g = 0.26 [0.19, 0.33]) of a small size. This effect did not vary according to type of disorder (anxiety vs. depression, p = .468). From a narrative review of experimental studies within clinical groups, four out of five studies reported statistically significant effects of manipulating the motor system on affective outcomes. DISCUSSION: This synthesis adds to the accumulating empirical evidence of motor alterations in depression and anxiety disorders. Future research will need to investigate how individuals suffering from depression or anxiety disorders could benefit from psychological, behavioral, and physical interventions directly aimed at the motor system.

How insulin-like growth factor I binds to a hybrid insulin receptor type 1 insulin-like growth factor receptor.

Monomers of the insulin receptor and type 1 insulin-like growth factor receptor (IGF-1R) can combine stochastically to form heterodimeric hybrid receptors. These hybrid receptors display ligand binding and signaling properties that differ from those of the homodimeric receptors. Here, we describe the cryoelectron microscopy structure of such a hybrid receptor in complex with insulin-like growth factor I (IGF-I). The structure (ca. 3.7 Å resolution) displays a single IGF-I ligand, bound in a similar fashion to that seen for IGFs in complex with IGF-1R. The IGF-I ligand engages the first leucine-rich-repeat domain and cysteine-rich region of the IGF-1R monomer (rather than those of the insulin receptor monomer), consistent with the determinants for IGF binding residing in the IGF-1R cysteine-rich region. The structure broadens our understanding of this receptor family and assists in delineating the key structural motifs involved in binding their respective ligands.

Emotion regulation patterns: Capturing variability and flexibility in emotion regulation in an experience sampling study.

Variability and flexibility in emotion regulation (ER) are considered important ingredients in adaptive ER. Few attempts at operationalizing variability and flexibility in ER have been made. In two 10-day experience sampling studies (N = 51 and 39), healthy participants rated their momentary emotions and their ER efforts in response to those emotions. We evaluated the association between ER (i.e., between and within ER strategy variability and ER flexibility, operationalized as putatively adaptive, putatively maladaptive and total strategies) and measures of well-being (psychological distress, satisfaction with life) in general (person-level) and in everyday life (day-level). Higher within-variability indicated that a strategy was used more at some occasions and less at others. Higher between-variability indicated variation in the extent to which different strategies were engaged at the same time point. Overall, results were mixed, but in some instances, indicators of ER variability and ER flexibility were related to each other and measures of well-being differently. Total within ER variability was negatively associated with well-being at the person and day level. Putatively adaptive between and within ER variability were associated with less well-being at the person level. At the day level, putatively adaptive and maladaptive between ER variability and maladaptive within ER variability were negatively associated with well-being. Putatively adaptive ER flexibility was negatively associated with satisfaction with life. This study adds to the literature on indicators of variability and flexibility in ER and their potential adaptiveness. The results indicate that variability in ER could be a maladaptive property, but more research is needed to understand this in terms of putatively adaptive and maladaptive strategies. Future studies on the adaptiveness of these indicators should obtain more contextual information.

Expansive and Contractive Postures and Movement: A Systematic Review and Meta-Analysis of the Effect of Motor Displays on Affective and Behavioral Responses.

This review and meta-analysis explores the experimental effects of expansive and contractive motor displays on affective, hormonal, and behavioral responses. Experimental studies were located through systematic literature searches. Studies had to manipulate motor displays to either expansive or contractive displays and investigate the effect of the displays on affect, hormones, or overt behavior. Meta-analyses were conducted to determine the pooled, standardized mean differences between the effects of motor displays on affective, hormonal, and behavioral responses. From 5,819 unique records, 73 relevant studies were identified. Robust differences between expansive and contractive displays emerged for affective responses and overt behavioral responses across contexts, type of manipulation, and methods of measurement. The results suggest that the effects are driven by the absence of contractive motor displays (contractive vs. neutral displays: Hedges's g = 0.45) rather than the presence of expansive displays (expansive vs. neutral displays: g = 0.06). The findings stand as a corrective to previous research, as they indicate that it is the absence of contractive displays rather than the presence of expansive displays that alters affective and behavioral responding. Future research should include neutral control groups, use different methods to assess hormonal change, and investigate these effects in the context of ideographic goals.

Is Negative Emotion Differentiation Associated With Emotion Regulation Choice? Investigations at the Person and Day Level.

Negative emotion differentiation (ED) has been suggested to be important for adaptive emotion regulation (ER). However, knowledge concerning how ED may impact specific ER strategy choice remains surprisingly sparse. We therefore investigated (1) if person-level negative ED was associated with habitual use of individual ER strategies, (2) how person-level negative ED was associated with daily use of individual ER strategies, and finally (3) how within-person daily fluctuations in negative ED were associated with daily use of individual ER strategies. During a 10-day experience sampling study, 90 healthy participants rated their momentary emotions and their ER efforts in response to those emotions. ER strategies included four putatively adaptive strategies (reflection, distancing, non-reactivity, reappraisal) and four putatively maladaptive strategies (rumination, experiential avoidance, expressive suppression, worry). Results revealed that negative ED at the person level was neither associated with habitual nor daily ER strategy endorsement when controlling for negative emotions. Likewise, associations between within-individual daily variation in negative ED and daily ER did not remain statistically significant after controlling for negative emotions. The results thus point to no or weak associations between negative ED and ER choice above and beyond negative emotions. Future experimental studies addressing ED at the momentary level and teasing out the ED-ER causal timeline are needed to further evaluate ED-ER associations. Findings from such research may represent an important step toward refining psychotherapeutic interventions aimed at improving emotional problems.

The impact of emotion regulation therapy on emotion differentiation in psychologically distressed caregivers of cancer patients.

BACKGROUND AND OBJECTIVES: Emotion differentiation is considered adaptive because differentiated emotional experiences are believed to promote access to the information that emotions carry, enabling context-appropriate emotion regulation. In the present study, secondary analyses from a recent randomized controlled trial (O'Toole et al., 2019) were conducted to investigate whether emotion differentiation can improve as a result of psychotherapy and whether improvements in emotion differentiation are associated with reduced distress. DESIGN AND METHODS: A total of 81 distressed caregivers of cancer patients were randomized to Emotion Regulation Therapy (ERT), an intervention aimed at improving emotion differentiation and facilitating healthy emotion regulation, or a waitlist condition. Emotion differentiation scores could be calculated for 54 caregivers. RESULTS: Repeated measures ANOVAs revealed that ERT led to significant improvements in negative (η2 = 0.21, p = .012), but not positive emotion differentiation (η2 = <0.01, p = .973). Correlation analyses showed that improvements in negative emotion differentiation were not associated with changes in distress. CONCLUSIONS: The results suggest that negative emotion differentiation can improve as a result of psychotherapy. Further research is needed to clarify how improvements in emotion differentiation following therapeutic interventions relate to treatment outcomes such as distress.Trial registration: ClinicalTrials.gov identifier: NCT02322905.

Developing a non-verbal, self-report assessment tool of action tendencies: The Depicted Action Tendencies (DAT) instrument.

The objectives of this series of studies were to develop and evaluate a visual self-report measure, the Depicted Action Tendencies (DAT), for the assessment of action tendencies, and to examine relations between action tendencies and emotions. In three independent studies, 938 participants evaluated drawings depicting individuals engaged in actions, reflecting four classes of action tendencies; the tendency to approach reward, approach threat, avoid threat, and avoid reward. The DAT instrument showed specificity in terms of associations with emotions and verbal action-tendency items with minimal overlap between drawings (Study 1). The specificity of the drawings extended to settings in which participants recalled events from their lives (Study 2 and 3). The findings suggest that the drawings can capture different action tendencies in contexts labeled with the same emotion and provide initial support for the DAT instrument as a valid and reliable measure of action tendencies. We believe the DAT instrument offers potential value both in experimental and clinical settings. Given its pictorial format, the DAT is easy to comprehend and may represent a valuable instrument for assessing action tendencies in a quick manner at the momentary level. It may also be useful to individuals with limited linguistic skills or difficulties in verbalizing sensations and feelings.

Phenoxyalkylimidazoles with an oxadiazole moiety are subject to efflux in Mycobacterium tuberculosis.

The phenoxyalkylimidazoles (PAI) are an attractive chemical series with potent anti-tubercular activity targeting Mycobacterium tuberculosis respiration. Our aim was to determine if the PAI compounds are subject to efflux. Two analogs containing an oxadiazole had improved potency in the presence of the efflux inhibitors reserpine and carbonyl cyanide m-chlorophenylhydrazine, whereas the potency of analogs with a diazole was not affected.

A systematic review and meta-analysis of the effect of emotion regulation on cortisol.

It is generally acknowledged that hormones are implicated in socioemotional behavior, yet little is known about the role of hormones in the context of emotion regulation. The aims of the present review and meta-analysis were to review and synthesize the available evidence pertaining to the effect of emotion regulation instructions on hormones, and to investigate whether this effect varies according to: type of hormone, context (e.g., emotion-induction procedure), emotion regulation characteristics (e.g., emotion regulation strategy), and presence and type of psychiatric disorder. PubMed, PsycINFO, and CINAHL were searched for experimental studies assessing the effect of instructed emotion regulation on levels of hormones (i.e., testosterone, cortisol, oxytocin, estradiol, and vasopressin) in physically healthy adults. The literature search yielded 17 relevant studies, 16 investigating cortisol and one investigating testosterone. Of these, 12 cortisol studies had eligible data for the meta-analysis. The results of the meta-analysis indicated no statistically significant effect of receiving an emotion regulation instruction compared with receiving no instruction on the cortisol response to subsequent emotion induction (g â€‹= â€‹-0.05, p â€‹= â€‹.48). However, within-person comparisons of change from an unregulated response to a regulated response indicated a significant change in cortisol levels (g â€‹= â€‹0.18, p â€‹= â€‹.03) consistent with the specified regulation goal (i.e., either up- or downregulation). No statistically significant effects were found in subgroup meta-analyses conducted according to context, emotion regulation characteristics or psychiatric disorders. Taken together, the findings indicate that emotion-induction procedures are associated with increases in cortisol that may subsequently return to equilibrium regardless of emotion-regulation instructions. Based on the large gaps in research (e.g., few studies investigated other hormones than cortisol, few studies included self-report measures of emotions) identified in the present review, we conclude that the effect of emotion regulation on hormones remains poorly understood. Prospero registration: CRD42020157336.

Introducing an In Vitro Liver Stability Assay Capable of Predicting the In Vivo Pharmacodynamic Efficacy of siRNAs for IVIVC.

There has been a renewed interest in therapeutic small interfering RNAs (siRNAs) over the past few years. This is particularly the result of successful and efficient delivery of N-acetylgalactosamine (GalNAc)-conjugated siRNAs to the liver. In general, the lead selection process for siRNA drugs is faster and more straightforward than traditional small molecules. Nevertheless, many siRNAs of different sequences and chemical modification patterns must still be evaluated before arriving at a final candidate. One of the major difficulties in streamlining this workflow is the well-known phenomenon that the in vitro data obtained from oligonucleotides transfected into cells are not directly predictive of their in vivo activity. Consequently, all oligonucleotides with some degree of in vitro activity are typically screened in vivo before final lead selection. Here, we demonstrate that the stability of liver-targeting GalNAc-conjugated siRNAs in a mouse liver homogenate shows an acceptable correlation to their in vivo target knockdown efficacy. Therefore, we suggest the incorporation of an in vitro liver homogenate stability assay during the lead optimization process for siRNAs. The addition of this assay to a flow scheme may decrease the need for animal studies, and it could bring cost savings and increase efficiency in siRNA drug development.

Thyroidectomy induces thyroglobulin formation by parotid salivary glands in rats.

Structural changes in parotid gland (PG) were previously reported following ablation of thyroid gland. However, the functional alterations (especially for proteins) have not been elucidated yet. Herein, we investigated the effect of rat thyroidectomy on PG structure and protein content and studied the ability of thyroxin-supplementation to alleviate the associated structural and functional changes. Male young adult 4-month old albino rats (n = 48) were allocated equally into 4 groups (control, sham-operated, thyroidectomized, and thyroxin-supplemented). PGs were examined histologically, and their proteins expression and localization were analyzed using western blot (WB) and immunohistochemistry (IHC), respectively at 3 w and 5 w post-surgery. Functionally, PGs of thyroidectomized rats formed a newly expressed 300 KDa protein, which was confirmed to be thyroglobulin (TG) by WB and IHC, with higher expression at 5 w. TG was localized in the interstitium, within capillaries, in the cytoplasm of the intralobular ductal cells, in the secretory products within the ductal lumen, and in the cytoplasm of individual small cells at the periphery of the acini. This functional change accompanied by structural changes in PGs (presence of dark and light acinar cells, TG-like colloid material, and high periductal vasculature). Noteworthy, PG of the thyroxin-supplemented depicted vanishment of TG. From these data, it could be concluded that thyroidectomy could alter the morphology and function of the parotid that induce a thyroid-like reprogramming of the parotid to secrete TG and thyroxin supplementation could alleviate this effect.

A 2,000-year-old specimen with intraerythrocytic Bartonella quintana.

Photogrammetry and cascading microscopy investigations of dental pulp specimens collected from 2,000-year-old individuals buried in a Roman necropolis in Besançon, France, revealed unprecedented preserved tissular and cellular morphology. Photogrammetry yielded 3-D images of the smallest archaeological human remains ever recovered. Optical microscopy examinations after standard haematoxylin-phloxine-saffron staining and anti-glycophorin A immunohistochemistry exposed dental pulp cells, in addition erythrocytes were visualised by electron microscopy, which indicated the ancient dental pulp trapped a blood drop. Fluorescence in situ hybridisation applied on red blood cells revealed the louse-borne pathogen Bartonella quintana, a finding confirmed by polymerase chain reaction assays. Through paleohistology and paleocytology, we demonstrate that the ancient dental pulp preserved intact blood cells at the time of the individual's death, offering an unprecedented opportunity to engage in direct and indirect tests to diagnose pathogens in ancient buried individuals.

POE Immunoassay: Plate-based oligonucleotide electro-chemiluminescent immunoassay for the quantification of nucleic acids in biological matrices.

Oligonucleotide therapeutics use short interfering RNA (siRNA) or antisense oligonucleotide (ASO) molecules to exploit endogenous systems-neutralizing target RNA to prevent subsequent protein translation. While the potential clinical application is vast, delivery efficiency and extrahepatic targeting is challenging. Bioanalytical assays are important in building understanding of these complex relationships. The literature currently lacks description of robust and sensitive methods to measure siRNA and ASOs in complex biological matrices. Described herein is a non-enzymatic hybridization-based immunoassay that enables quantification of individual siRNA strands (antisense or sense) in serum, urine, bile, and liver and kidney homogenates. Assay utility is also demonstrated in ASOs. The assay improves upon previous works by abolishing enzymatic steps and further incorporating Locked Nucleic Acid (LNA) nucleotide modifications to increase analyte hybridization affinity and improve sensitivity, specificity, and robustness. We report an assay with an ultrasensitive dynamic range of 0.3 to 16,700 pM for siRNA in serum. The assay was submitted to full qualification for accuracy and precision in both serum and tissue matrices and assay performance was assessed with single and mixed analytes. The reliable LNA-hybridization-based approach removes the need for matrix sample extraction, enrichment or amplification steps which may be impeded by more advanced chemical modifications.