Serological evidence of exposure to Toxoplasma gondii infection in wild birds and local chickens (Gallus gallus domesticus) in Plateau State, North Central Nigeria.

Toxoplasma gondii, the cause of toxoplasmosis is an obligate intracellular protozoan parasite of veterinary and public health importance. Ground feeding birds play an active role in the epidemiology of the disease and are the best indicators of soil contamination with the oocysts. Thirteen (14.1%; 95% CI = 7.7-23.0) of the 92 wild birds (6 of Coliformes; 4 of Columbiformes; 67 of Passeriformes and 15 of Piciformes) captured from Shere hills and 43 (9.3%; 95%CI = 4.9-12.4) of the 460 local chickens obtained across 3 senatorial regions of Plateau State, Nigeria were positive for anti-T. gondii antibodies using enzyme linked immunosorbent assay (ELISA). Varying prevalence (16.7-100%) of anti-T. gondii antibodies were recorded across 10 of 35 wild bird species; 2 (28.6) of 7 African thrush (Turdus pelios), 2 (33.3) of 6 speckled mouse bird (Colius striatus), 1 (20.0%) of 5 speckled fronted weaver (Sporopipes frontalis), 1 (33.3%) of 3 Northern Red bishop (Euplectes franciscanus), 1 (50.0%) of 2 village weaver (Ploceus cucullatus), 2 (28.6%) of 7 common bulbul (Pycnonotus barbatus), 1 (50.0%) of 2 black crowned tchagra (Tchagra senegalus), 1 (50.0%) of 2 black billed wood dove (Turtur abyssinicus), 1 (100.0%) of 1 mocking cliff chat (Thamnolaea cinamomeiventris), 1 (16.7) of 6 vieillot's barbet (Lybius vieilloti). The prevalence did not vary significantly based on the sex (p = 0.14), feeding guild (p = 0.53) and the taxonomic order (p = 0.62) of the wild birds. Regional based prevalence among local chickens ranged between 8.0 and 11.8% across the 3 senatorial zones. The source (p = 0.513; 95% CI = 4.9-16.8) and age (p = 0.797; 95% CI = 4.9-16.8) of local chickens were not associated with T. gondii seropositivity. Seropositivity was higher in the females (95% CI = 9.1-18.3) than in the males (p = 0.004; 95% CI = 3.0-9.4). This is the first report of the serological evidence of exposure to T. gondii infection in a wide variety of wild birds and local chickens in the area. It suggest that birds in this region are important asymptomatic intermediate hosts and play a role in the maintenance of the organism in the ecosystem.

Dog ecology and its implications for rabies control in Gwagwalada, Federal Capital Territory, Abuja, Nigeria.

The objectives of this study were to determine the characteristics of a dog population, including their accessibility to vaccination and health care, in urban and semiurban areas of Gwagwalada, Abuja, Nigeria. Direct street counts and a house-to-house survey of city streets were performed. A total of 451 households were surveyed comprising 43.7% urban and 53.3% semiurban areas. A total of 848 owned dogs were identified, along with 3,115 corresponding humans. With a dog-to-human ratio of 1:3.7, the dog population in the study area was estimated as 103,758. A total of 396 dogs were counted on the streets with the greater proportion (74%) in semiurban areas. Most dogs in semiurban areas (77.3%) had no certificate confirming vaccination against rabies, compared to 47.2% in urban areas (p = .004). The majority of dogs in the urban (60.9%) and semiurban (82.0%) were free roaming. In the multivariable model, age, presence of a collar, region, sex, use and having ever visited a veterinarian were significantly associated with rabies vaccination. The majority (125/197, 63.5%) of respondents with higher education were willing to pay more for the healthcare needs of their dogs as opposed to those with a lower level of education (93/251, 37.1%, p = .001). The study revealed a high dog population density, vaccination coverage below WHO recommendation of 70% and generally reduced healthcare-seeking behaviour among dog owners in Gwagwalada, Abuja, Nigeria.

Detection of rabies antigen in the saliva and brains of apparently healthy dogs slaughtered for human consumption and its public health implications in abia state, Nigeria.

The study was carried out in eight dogs slaughtering outlets within four Local Government Areas of the State for the determination of rabies antigen in the saliva and brain of apparently healthy dogs slaughtered for human consumption. A total of one hundred (100) samples each of saliva and brain were collected before and after slaughter, respectively, between April to June, 2013, in the selected areas. The saliva was subjected to rapid immune-chromatographic test (RICT) while direct fluorescent antibody test (DFAT) was carried out on the brain samples. Structured questionnaire was administered to nineteen (19) dog meat processors comprising 18 males and 1 female in the selected areas. Sixty four percent of the samples tested were from female dogs while 36% were from males, 5% tested positive for rabies antigen with the use of both tests; there was no statistical association between sex and rabies status of the dogs sampled (P > 0.05). Butchers bitten during the course of slaughtering were 94.7% out of which 72.8% utilized traditional method of treatment and only 27.8% reported to the hospital for proper medical attention. This study has established the presence of rabies antigen in apparently healthy dogs in the study area.

Public health implications of soil contaminated with helminth eggs in the metropolis of Kaduna, Nigeria.

Environmental and socio-cultural variables influencing the distribution of helminth eggs in 608 soil samples were studied in 14 playgrounds that differ in socio-economic status in Kaduna metropolis, Nigeria, using a modified sieving method and a sucrose flotation medium of specific gravity 1.27. Helminth eggs were found in 62% of the soil samples and the distribution was as follows: Toxocara spp. 50.4%, Taenia spp./Echinococcus spp. 36.9%, Dipylidium caninum 26.3%, Ancylostoma spp. 9.0%, Ascaris spp. 7.2%, Trichuris spp. 3.7% and Ascaridia spp. 1.9%. A higher prevalence (68.1%) was recorded during the dry harmattan period while in the rainy period the rate was 58.1%. Mean egg densities ranged from 1.11 +/- 0.32 to 3.92 +/- 2.47 in areas moderately rated. Samples from site 14, which was highly rated, were more contaminated (78.1%) than those collected from other sites, while the intensity of contamination (14.0%) was more in moderately rated site 4 than in the rest of the sites. There were significant associations between the prevalence of helminth eggs and rainy period of the study (odds ratio (OR) = 0.38; 95% confidence interval (CI) on OR: 0.20 0.05). This study shows that the period of study, the presence of dogs and vegetation influence the prevalence of helminth eggs in soil in Kaduna metropolis.