Distribution, abundance, population structures, and potential impacts of the invasive snail, Tarebia granifera in aquatic ecosystems of north-eastern South Africa.

Aquatic ecosystems globally have been invaded by molluscs. Tarebia granifera is a highly successful invader, often becoming the dominant aquatic invertebrate species in an invaded ecosystem. Resultingly, it has been suggested that T. granifera may have severe negative impacts on these invaded ecosystems. Limited information is available regarding the population structures and densities of T. granifera, particularly in invaded countries such as South Africa, and information on this could assist in developing management and control strategies for this invasive species. The present study aimed to assess the current distribution, densities, and population structures of T. granifera in invaded habitats on the Limpopo and Phongolo River systems in South Africa. This was accomplished by collecting aquatic molluscs from sites across these systems. Water quality parameters were measured at each site and water samples were collected for chemical nutrient analyses. The density of snails was determined for each site and the population size and structure as well as birth rate was calculated for T. granifera. Tarebia granifera was found to be the dominant molluscan species in habitats where it was present and all size classes from newborn to mature adults were found throughout at some of the highest densities globally. Worryingly, native molluscan species, were often absent or in much lower densities than reported in literature at sites where T. granifera was present, suggesting a negative effect on the native molluscan density and diversity. Contrary to most previous studies, there were no significant correlations between T. granifera and the selected water quality parameters. Higher densities and newborn recruitment of T. granifera were observed in the spring than in autumn, likely in response to shifts in environmental conditions. This study provides crucial insights into the population structure, densities, and impacts of T. granifera in invaded habitats, particularly for relatively newly invaded regions such as southern Africa.

Morphological description and molecular characterisation of Dactylogyrus matlopong sp. n. (Monogenea: Dactylogyridae) from the South African endemic Labeobarbus aeneus (Cyprinidae: Torinae).

Dactylogyrus Diesing, 1850 is the most species-rich genus in Platyhelminthes, with over 900 documented species, that are mostly strictly specific to freshwater cyprinoids. The morphological Dactylogyrus groups afrobarbae-type, carpathicus-type, pseudoanchoratus-type, and varicorhini-type are known to occur in Africa. This study describes a new species of Dactylogyrus of the varicorhini-type from the gills of the endemic smallmouth yellowfish Labeobarbus aeneus (Burchell) from the Vaal River, Free State Province, South Africa. Dactylogyrus matlopong sp. n. is unique among its varicorhini-type congeners mainly by the accessory piece of the male copulatory complex that presents a hook-shaped subunit with defined round base not reaching the male copulatory organ, combined with the presence of a conspicuous medial projection on the anterior margin of the ventral bar. Sequences of the partial 28S and 18S rRNA genes, together with entire ITS1 region, were generated for the first time for a species of Dactylogyrus from South Africa. Concatenated phylogenetic analyses of selected Dactylogyrus spp. showed that these parasites group according to their morphological types.

Description of Gyrodactylus paludinosus sp. nov. (Monogenea: Gyrodactylidae) from the Straightfin Barb, Enteromius paludinosus (Peters, 1852), in South Africa.

INTRODUCTION: Parasites of the genus Gyrodactylus von Nordmann, 1832, are predominantly parasites of freshwater and marine bony fishes. To date, 40 species of the genus have been described from Africa, with only 6 of these reported from South Africa. This represents only a small fraction of the known diversity of almost 500 species known worldwide. METHODS: In total, 43 of Straighhfin barb, Enteromius paludinosus were collected from Barberspan Bird Sanctuary, North West Province, South Africa. Monogenean parasites were isolated from the fins and fixed on microscope slides in ammonium picrate glycerine. Morphometric evaluation of opisthaptoral hard parts confirmed the identity of these specimens as a new species. RESULTS: The description of Gyrodactylus paludinosus sp. nov. is presented. The new species has well-developed, slightly curved superficial inner roots and well-developed rectangular-like ventral bar membrane. Despite the small differences between the hamuli size, it can be distinguished from its congeners, G. kyogae and G. ivindoensis, the only species known from small African barbs, by the shape of ventral bars and marginal hook sickle propers. CONCLUSION: The description of Gyrodactylus paludinosus n. sp. increases the total number of Gyrodactylus spp. known from African cyprinid fish to three. Undoubtedly, more attention should be given to studies on small barbs as potential hosts for new species.

The role of fish helminth parasites in monitoring metal pollution in aquatic ecosystems: a case study in the world's most productive platinum mining region.

Due to the increasing consumption of platinum (Pt), especially in automobile exhaust catalysts, environmental concentrations of Pt are of emerging concern worldwide. Limited information exists on environmental concentrations, particularly in Pt mining regions, while South Africa is the world's main supplier of Pt. Moreover, other metals are also released as by-products of Pt mining, which might also cause environmental concern. Certain fish parasite taxa have the ability to accumulate metals orders of magnitude higher than their hosts and can be used to reliably detect metals with naturally low abundance. Studies on Pt accumulation in parasite-host systems are limited. Therefore, the aims of the present study were (1) to determine the accumulation of a variety of metals (cadmium (Cd), chromium (Cr), copper (Cu), nickel (Ni), lead (Pb), platinum (Pt), and zinc (Zn)) in helminth fish parasites compared with their hosts from a reference site and an impoundment impacted by Pt mining activities; (2) to assess whether there is a difference between bioaccumulation of metals in infected and uninfected hosts, as well as between hosts with different infection intensities; and (3) to compare the biomarker responses (acetylcholine esterase activity (AChE), metallothionein content (MT), catalase activity (CAT), reduced glutathione content (GSH), malondialdehyde content (MDA), protein carbonyls induction (PC), superoxide dismutase activity (SOD), and cellular energy allocation (CEA)) between infected and uninfected hosts. The cestode Atractolytocestus huronensis accumulated significantly higher concentrations of Cr, Ni, and Pt than their host Cyprinus carpio, while the nematode Contracaecum sp. accumulated significantly higher concentrations of Pt and Zn than their host Clarias gariepinus. Infected fish showed lower metal concentrations compared to uninfected fish, while the parasites had no significant effects on their hosts' biomarker responses. The parasites demonstrated the bioavailability of metals derived from Pt mining activities and their ability to resist its toxic effects. Thus, these parasites are promising sensitive accumulation indicators for Cr, Ni, Pb, and Pt contaminations from Pt mining activities.

Understanding growth relationships of African cymothoid fish parasitic isopods using specimens from museum and field collections.

Cymothoid isopods are a diverse group of ectoparasites of fish species, and are particularly conspicuous as they are large and attach to the body surface, mouth, and gill chamber of fish hosts. These parasites transition from juvenile to male to female, and how their size changes with ontogeny and correlates with host size is not well understood. To better understand these relationships, data from field and museum collected samples of South Africa were combined to test for the associations between host and parasite length for three mouth and one gill chamber-infesting genera (Ceratothoa, Cinusa, Cymothoa, and Mothocya respectively). Generally, the number of parasites collected from 90 h of museum surveying was similar to that of seven, one-week long field collections. For two of the three mouth-infesting parasites, parasite and host size were significantly and positively correlated for males and females, but not juveniles. For gill chamber-infesting parasites, female and male parasite sizes were weakly and not significantly correlated with host size. These results provide the first morphometric data and growth relationship data for African cymothoid species and their fish hosts, and demonstrate the value and efficiency of using museum collections in ecological research.

The Application of a Macroinvertebrate Indicator in Afrotropical Regions for Pesticide Pollution.

Many biotic integrity indices are not able to isolate community effects due to pesticide exposure as the communities also respond to other anthropogenic and natural stressors. A macroinvertebrate trait bioindicator system that is pesticide specific was therefore developed to overcome these challenges. This system, called SPEAR (SPEcies At Risk), was applied in South Africa as an indicator to link known pesticide catchment usage to changes in the macroinvertebrate community, especially when analytical methods are inconclusive. In addition, the SPEARsalinity index within the SPEAR suite of tools was also evaluated for its effectiveness in South Africa. The results indicated that all of the sites have either been exposed to the same pesticide pressure or not been exposed to pesticides as the SPEAR results were similar when compared to the pesticide intensity. The interaction with other factors like nutrients or salinity was likely a factor that confounded the SPEARpesticides indicator.

Distribution of perfluorinated compounds (PFASs) in the aquatic environment of the industrially polluted Vaal River, South Africa.

Perfluorinated alkyl substances (PFASs) are highly persistent chemicals, which have a bioaccumulative potential and can be found in wildlife around the world. Although multiple studies have been performed on PFASs pollution of the aquatic environment, little is known on PFASs pollution on the African continent and their possible risks for human health. In the present study, we examined the distribution of 15 PFASs in fish, invertebrates, sediment and water, collected at three sites, representing a gradient of industrial and mining pollution, along the Vaal River, South Africa. Furthermore, possible risks for human health through consumption of contaminated fish were examined. Perfluorooctane sulfonate (PFOS) was the most dominant PFAS measured in biota, whereas perfluoropentanoic acid (PFPeA) was measured in higher concentrations in water. Mean PFAS concentrations in water ranged from

Alien freshwater fish parasites from South Africa: Diversity, distribution, status and the way forward.

The global translocation and introduction of freshwater fish into non-native regions has created the perfect opportunity for the co-introduction of their parasites. In a recent review on non-native freshwater fish introductions in South Africa, 55 fishes were reported as introduced into novel environments in South Africa, with 27 alien and 28 extralimital. However, the parasites potentially co-introduced by these non-native fishes have received much less attention from researchers than the hosts themselves. Thus far, the only attempts at summarising our knowledge on the diversity of introduced freshwater fish parasites in this region dates back to the 1980s when only four parasite species were considered to be alien, with a further eight species as doubtful. Over the last thirty years, more records have been added and this paper aims to provide an up-to-date review of our knowledge on the diversity, distribution, status (co-invasive or co-introduced) and the direction for future studies on introduced freshwater fish parasites in South Africa. Here we consider seven species (four ciliates, and one cestode, copepod and branchiuran respectively) as confirmed co-invaders, and 16 species (one flagelate, four ciliates, one cestode and ten monogeneans) as co-introduced. In addition, six species (three ciliates, two monogeneans and one copepod) previously recorded as invasive are deemed to be of uncertain status, and one ciliate is removed from the list of known invasive parasites from this region. It is further proposed that future research should focus on extralimital co-introductions, especially in the Eastern and Western Cape regions of South Africa where more than half of the fishes present are introduced species. It is also recommended that all new records of introduced parasites and new distribution records of known invasive parasites should include the deposition of voucher specimens in museums and, as far as possible, include molecular confirmation of its identification.