Color variations in PMMA acrylic resins after thermocycling and mouthwash simulation / Variações de cor em resinas acrílicas de PMMA após termociclagem e simulação de enxaguatório bucal

Estabilidade de cor é um requisito estético fundamental das resinas acrílicas provisórias, em especial quando esses materiais são submetidos a longos períodos na cavidade oral. Embora resinas acrílicas temporárias novas e aprimoradas estejam disponíveis, os efeitos da variação térmica e de soluções antissépticas na estabilidade de cor de resinas acrílicas provisórias ainda não foram completamente elucidados. Portanto, este estudo avaliou a coloração de resinas autopolimerizáveis polimetilmetacrilato (PMMA) e bis-acrilo submetidas à termociclagem e imersão em clorexidina. Amostras padronizadas (n=10) foram preparadas de duas resinas acrílicas temporárias a base de PMMA (Alike® e Duralay®). Foram realizadas três avaliações de cor (T1 ­ 24h após o preparo da amostra, T2 ­ após termociclagem e T3 ­ após termociclagem e imersão em clorexidina) por meio de espectrofotômetro eletrônico de seleção de sombra (Vita Easy Shade). Os dados obtidos foram analisados por ANOVA e teste t (α=0,05). As resinas de PMMA Alike e Duralay apresentaram alteração de cor após a termociclagem e também após a imersão em clorexidina.

Color stability is a fundamental aesthetic requirement for temporary acrylic resins, especially when these materials are subjected to long periods in the oral cavity. Although new and improved temporary acrylic resins are available, the effects of thermal variation and antiseptic solutions on the color stability of temporary acrylic resins have not yet been fully elucidated. Therefore, this study evaluated the color of self-polymerizing resins polymethyl methacrylate (PMMA) and bis-acryl subjected to thermocycling and immersion in chlorhexidine. Standardized samples (n=10) were prepared from two PMMA-based temporary acrylic resins (Alike® and Duralay®). Three color evaluations were carried out (T1 ­ 24h after sample preparation, T2 ­ after thermocycling and T3 ­ after thermocycling and immersion in chlorhexidine) using an electronic shade selection spectrophotometer (Vita Easy Shade). The data obtained were analyzed by ANOVA and t test (α=0.05). PMMA Alike and Duralay resins showed color changes after thermocycling and also after immersion in chlorhexidine.

IN VITRO ANTIMICROBIAL ACTIVITY OF Schinus terebinthifolia Raddi METRONIDAZOLE, CLINDAMYCIN AGAINST Lactobacillus gasseri Lauer and Kandler AND ITS IMPLICATIONS IN RECURRENT BACTERIAL VAGINOSIS / ATIVIDADE ANTIMICROBIANA DE Schinus terebinthifolia Raddi METRONIDAZOL, CLINDAMICINA E CONTRA Lactobacillus gasseri Lauer and Kandler, IN VITRO, E SUAS IMPLICAÇÕES NA VAGINOSE BACTERIANA RECIDIVANTE

Bacterial vaginosis (BV) is very prevalent and is the most common cause of vaginal discharge worldwide. It is a dysbiosis resulting from the replacement of hydrogen peroxide and lactic acid-producing lactobacilli by anaerobic bacteria in high concentrations, including Gardnerella vaginalis, Prevotella, Mobiluncus, Atopobium and other anaerobes. BV can be self-diagnosed when the patient presents the classic symptoms, or clinically and laboratory-based, following the Amsel criteria or by determining the Nugent score. The recommended treatments for BV are oral metronidazole, secnidazole and tinidazole, as well as vaginal metronidazole gel, clindamycin cream and Schinus terebinthifolia Raddi gel. The present study aimed to determine the ability of vaginal clindamycin cream, vaginal metronidazole gel and S. terebinthifolia Raddi vaginal gel to inhibit the growth or preserve the population of Lactobacillus gasseri ATCC 19992 microorganisms in vitro. The methodology used was perforation in plates, forming 6 mm wells, where the samples were inoculated. The plates were incubated for 48 hours at 30°C. After this period, by visual plates analysis, was observed that L. gasseri is resistant to S. terebinthifolia Raddi vaginal gel since no inhibition halo was observed. However, L. gasseri was moderately susceptible to Metronidazole vaginal gel and highly sensitive to Clindamycin vaginal cream. As lactobacilli, of which L. gasseri stands out, represent over 95% of the microorganisms 3/16 that inhabit the vaginal environment, maintaining the health of this microbiome, the action of Clindamycin and Metronidazole can lead to an imbalance in the vaginal microbiota, allowing relapses when these therapeutic agents are used to treat BV.

A vaginose bacteriana (VB) é muito prevalente e é a causa mais comum de corrimento vaginal em todo o mundo. Trata-se de uma disbiose resultante da substituição dos lactobacilos produtores de peróxido de hidrogênio e ácido lático por bactérias anaeróbicas em altas concentrações, incluindo Gardnerella vaginalis, Prevotella, Mobiluncus, Atopobium e outros anaeróbios. A VB pode ser autodiagnosticada, quando a paciente apresenta a sintomatologia clássica, ou clínica e laboratorialmente obedecendo aos critérios de Amsel ou pela determinação do escore de Nugent. Os tratamentos recomendados para a VB são o Metronidazol, o Secnidazol e o Tinidazol por via oral, bem como o metronidazol gel, a clindamicina creme e o gel de S. terebinthifolia Raddi por via vaginal. O presente estudo teve por objetivo determinar a capacidade da Clindamicina creme vaginal, do Metronidazol gel vaginal e do S. terebinthifolia Raddi gel vaginal em inibir o crescimento ou preservar a população dos microrganismos Lactobacillus gasseri ATCC 19992 in vitro. A metodologia utilizada foi de perfuração em placa, formando poços de 6mm, onde as amostras foram inoculadas. As placas foram incubadas por 48 horas à temperatura de 30°C. A análise visual das placas, após o período de incubação, evidenciou que L. gasseri é resistente ao gel vaginal de S. terenbithifolia Raddi gel, uma vez que não foi observado qualquer halo de inibição. Contudo, L. gasseri foi moderadamente susceptível ao gel vaginal de Metronidazol e altamente sensível ao creme vaginal de Clindamicina. Como os lactobacilos, dos quais se destaca L. gasseri, representam acima de 95% dos microrganismos que habitam o meio vaginal, mantendo a higidez desse microbioma, a ação da Clindamicina e do Metronidazol pode levar ao desequilíbrio da microbiota vaginal, permitindo as recidivas quando esses agentes terapêuticos são usados para o tratamento da VB.

Prognostic factors for local recurrence in patients with rectal cancer submitted to neoadjuvant chemoradiotherapy and total mesorectal excision.

Prognostic factors for local recurrence in patients with rectal cancer submitted to neoadjuvant chemoradiotherapy and total mesorectal excision. BACKGROUND: The standard curative treatment for locally advanced rectal cancer of the middle and lower thirds is long-course chemoradiotherapy followed by total mesorectal excision. PURPOSE: To evaluate the prognostic factors associated with local recurrence in patients with rectal cancer submitted to neoadjuvant chemoradiotherapy and total mesorectal excision. METHODS: Retrospective study including patients with rectal cancer T3-4N0M0 or T (any)N + M0 located within 10 cm from the anal border, or patients with T2N0M0 located within 5 cm, treated by long course chemoradiotherapy followed by total mesorectal excision with curative intent. Clinical, demographic, radiologic, surgical, and anatomopathological data were collected. Local recurrence was estimated using the Kaplan-Meier function, and risk was estimated according to each characteristic using univariate and multivariate analyses. RESULTS: 270 patients were included, 57.8% male and mean age 61.7 (30‒88) years. At initial staging, 6.7% of patients were stage I, 21.5% stage II, and 71.8% stage III. Open surgery was performed in 65.2%, with sphincter preservation in 78.1%. Mortality within 30 postoperative days was 0.7%. After 49.4 (0.5‒86.1) months of median follow-up, overall and local recurrences were 26.3% and 5.9%. On multivariate analyses, local recurrence was associated with involvement of the mesorectal fascia on restaging MRI (HR = 9.11, p = 0.001) and with pathologic involvement of radial surgical margin (HR = 8.19, p < 0.001). CONCLUSION: Local recurrence of rectal cancer treated with long-course chemoradiation and total mesorectal excision is low and is associated with pathologic involvement of the radial surgical margin and can be predicted on restaging MRI.

Thickness determination of hydroperoxidized lipid bilayers from medium-resolution cryo-TEM images.

As the primary products of lipid oxidation, lipid hydroperoxides constitute an important class of lipids generated by aerobic metabolism. However, despite several years of effort, the structure of the hydroperoxidized bilayer has not yet been observed under electron microscopy. Here we use a 200 kV Cryo-TEM to image small unilamellar vesicles (SUVs) made (i) of pure POPC or SOPC, (ii) of their pure hydroperoxidized form, and (iii) of their equimolar mixtures. We show that the challenges posed by the determination of the thickness of the hydroperoxidized bilayers under these observation conditions can be addressed by an image analysis method that we developed and describe here.

Safety and Biodistribution of an Autologous Bone Marrow-Derived Mononuclear Cell Infusion into Renal Arteries in Patients with Focal Segmental Glomerulosclerosis: A Phase 1 Study.

Patients with focal segmental glomerulosclerosis (FSGS) who are refractory to drug treatment may present progressive loss of kidney function, leading to chronic kidney disease stage 5 under dialysis treatment. The safety of systemic administration of bone marrow-derived mononuclear cells (BMDMCs) has been shown in different preclinical models of kidney diseases. However, to date, no study has evaluated the safety and biodistribution of BMDMCs after infusion in renal arteries in patients with FSGS. We used a prospective, non-randomized, single-center longitudinal design to investigate this approach. Five patients with refractory FSGS and an estimated glomerular filtration rate (eGFR) between 20 and 40 ml/min/1.73 m2 underwent bone marrow aspiration and received an arterial infusion of autologous BMDMCs (5 × 107) for each kidney. In addition, BMDMCs labeled with technetium-99m (99mTc-BMDMCs) were used to assess the biodistribution by scintigraphy. All patients completed the 270-day follow-up protocol with no serious adverse events. A transient increase in creatinine was observed after the cell therapy, with improvement on day 30. 99mTc-BMDMCs were detected in both kidneys and counts were higher after 2 hr compared with 24 hr. The arterial infusion of BMDMCs in both kidneys of patients with FSGS was considered safe with stable eGFR at the end of follow-up. This trial is registered with NCT02693366.

Nanofluidic resistive pulse sensing for characterization of extracellular vesicles.

This paper describes the development, design and characterization of a resistive pulse sensing (RPS) system for the analysis of size distributions of extracellular vesicles (EVs). The system is based on microfluidic chips fabricated using soft-lithography and operated in pressure-driven mode. This fabrication approach provided reproducible pore dimensions and the best performing chip design enabled, without calibration, sizing of both 252 nm and 460 nm test particles within 8% of theoretically calculated values, based on the size specifications provided by suppliers. The number concentration measurement had higher variations and without calibration provided estimates within an order of magnitude, for sample concentrations across 4 orders of magnitude. The RPS chips could also measure successfully EVs and other biological nanoparticles in purified samples from cell culture media and human serum. A compact, fast and inexpensive RPS system based on this design could be an attractive alternative to current gold-standard techniques for routine characterization of EV samples.

POF-based biosensors for cortisol detection in seawater as a tool for aquaculture systems.

A surface plasmon resonance (SPR) phenomenon implemented via D-shaped polymer optical fiber (POF) is exploited to realize cortisol biosensors. In this work, two immonosensors are designed and developed for the qualitative as well as quantitative measurement of cortisol in artificial and real samples. The performances of the POF-based biosensors in cortisol recognition are achieved using different functionalization protocols to make the same antibody receptor layer over the SPR surface via cysteamine and lipoic acid, achieving a limit of detection (LOD) of 0.8 pg/mL and 0.2 pg/mL, respectively. More specifically, the use of cysteamine or lipoic acid changes the distance between the receptor layer and the SPR surface, improving the sensitivity at low concentrations of about one order of magnitude in the configuration based on lipoic acid. The LODs of both cortisol biosensors are achieved well competitively with other sensor systems but without the need for amplification or sample treatments. In order to obtain the selectivity tests, cholesterol and testosterone were used as interfering substances. Moreover, tests in simulated seawater were performed for the same cortisol concentration range achieved in buffer solution to assess the immunosensor response to the complex matrix. Finally, the developed cortisol biosensor was used in a real seawater sample to estimate the cortisol concentration value. The gold standard method has confirmed the estimated cortisol concentration value in real seawater samples. Liquid-liquid extraction was implemented to maximize the response of cortisol in liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis.

Introduction to the feature issue: Advances in Optical Biosensors for Biomedical Applications.

The feature issue of Biomedical Optics Express titled "Advances in Optical Biosensors for Biomedical Applications" presents a comprehensive collection of cutting-edge optical biosensor research. With the growing demand for sensitive, label-free, and real-time detection of biological analytes, optical biosensors have emerged as important devices in a wide range of biomedical applications, including medical diagnostics, bioanalysis, and personalised healthcare. This collection of 26 papers highlights recent advances and innovations in the development, design, and implementation of optical biosensors. The feature issue serves as an opportunity for disseminating ground-breaking findings, promoting new ideas, and inspiring further developments in optical biosensors for medical applications. The authors provide breakthrough technology, innovative approaches, and practical clinical applications that have the potential to revolutionize healthcare and biomedical research.

Highly stabilized fiber Bragg grating accelerometer based on cross-type diaphragm.

A fiber Bragg grating (FBG) accelerometer based on cross-type diaphragm was proposed and designed, in which the cross-beam acts as a spring element. To balance the sensitivity and stability, the accelerometer structure was optimized. The experimental results show that the designed device has a resonant frequency of 556 Hz with a considerable wide frequency bandwidth of up to 200 Hz, which is consistent with the simulation. The sensitivity of the device is 12.35 pm/g@100 Hz with a linear correlation coefficient of 0.99936. The proposed FBG accelerometer has simple structure and strong anti-interference capability with a maximal cross-error less than 3.26%, which can be used for mechanical structural health monitoring.

Is religiosity/spirituality in patients with Crohn's disease important to their quality of life?

The authors aim to study Religiosity/Spirituality (R/S) and Quality of Life (QoL) in patients with Crohn's disease and their correlation with the disease phenotypes. METHODS: Prospective cross-sectional cohort study with 151 consecutive patients enrolled from March 2021 to October 2021 at the Colorectal IBD Outpatient of Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP). Sociodemographic, Religiosity/Spirituality (Duke University Religion Index - Durel) questionnaires and QoL (Inflammatory Bowel Disease Questionnaire - Short IBDQ-S) were applied. When necessary, qualitative variables were evaluated using the chi-square or Fisher's exact test. The Mann-Whitney and Kruskall-Wallis tests were used to analyze quantitative variables and compare more than two groups, both non-parametric statistical techniques. RESULTS: The most frequent location was Ileocolonic followed by Ileal and colonic (41.1 %, 27.2 %, and 25.2 %); only 6.6 % of subjects had a perianal presentation. Inflammatory, stenosing, and penetrating behaviors showed 36.4 %, 19.1 %, and 44.4 % respectively. The majority of the population is Catholic, Evangelical, or Spiritualist (92.4 %). QoL score showed no significant difference in the phenotypes. The scores for DUREL domains were 61.4 % for organizational religiosity, 75 % for non-organizational religiosity, 98.6 %, 93.6 % and 89.3 % for intrinsic spirituality, with high results in all disease phenotypes. CONCLUSIONS: The studied population presented homogeneous sociodemographic results and high religious and spiritual activity. R/S in a positive context were not associated with better QoL or phenotype. R/S is present in the patients' lives and could be seen as an important tool for adherence to treatment and the professional relationship between doctor and patient. The homogeneity of the sample difficult for an appropriate evaluation, which leads us to suggest new studies with more heterogeneous groups.

Characterization of optical fibers doped with nanoparticles for distributed displacement sensing.

High-scattering optical fibers have emerged as a key component in distributed sensing systems, primarily due to their capacity to enhance signal-to-noise ratio. This paper presents an experimental characterization of optical fibers doped with oxide nanoparticles for displacement sensing. They were manufactured using the phase-separation technique and different doping compounds, including calcium, strontium, lanthanum and magnesium. The Rayleigh backscattering (RBS) signatures in time and frequency domains were acquired using an Optical Backscatter Reflectometer (OBR). The maximum representative length, backscattering gain and strain sensitivity were evaluated. The results indicate that the fiber co-doped with magnesium and erbium chlorides offered the best compromise between strain sensitivity (0.96 pm/µ ϵ) and maximum length (17 m). For conditions of single and multiple perturbations, strain saturation was reached at ≥7000 µm and <1500 µm, respectively. In addition, the results reveal that, under a condition of variable temperature (30-60 °C), the sensor response becomes significantly nonlinear over length, requiring a technique for temperature cross-sensitivity mitigation that accounts for nonlinearities in sensitivity and hysteresis.

Precise detection of trace level protein using MIP-MoS2 nanocomposite functionalized PCF based interferometer.

Fiber optic interferometry combined with recognizing elements has attracted intensive attention for the development of different biosensors due to its superior characteristic features. However, the immobilization of sensing elements alone is not capable of low-concentration detection due to weak interaction with the evanescent field of the sensing transducer. The utilization of different 2D materials with high absorption potential and specific surface area can enhance the intensity of the evanescent field and hence the sensitivity of the sensor. Here, a biosensor has been fabricated using an inline hetero fiber structure of photonic crystal fiber (PCF) and single-mode fiber (SMF) functionalized with a nanocomposite of molybodenum di-sulfide (MoS2) and molecular imprinting polymer (MIP) to detect trace levels of bovine serum albumin (BSA). The sensor showed a wide dynamic detection range with a high sensitivity of 2.34 × 107 pm/µg L-1. It shows working potential over a wide pH range with a subfemtomolar detection limit. The compact size, easy fabrication, stable structure, long detection range, and high sensitivity of this sensor would open a new path for the development of different biosensors for online and remote sensing applications.

Optical fiber immunosensors based on surface plasmon resonance for the detection of Escherichia coli.

Every year, millions of people suffer some form of illness associated with the consumption of contaminated food. Escherichia coli (E. coli), found in the intestines of humans and other animals, is commonly associated with various diseases, due to the existence of pathogenic strains. Strict monitoring of food products for human consumption is essential to ensure public health, but traditional cell culture-based methods are associated with long waiting times and high costs. New approaches must be developed to achieve cheap, fast, and on-site monitoring. Thus, in this work, we developed optical fiber sensors based on surface plasmon resonance. Gold and cysteamine-coated fibers were functionalized with anti-E. coli antibody and tested using E. coli suspensions with concentrations ranging from 1 cell/mL to 105 cells/mL. An average logarithmic sensitivity of 0.21 ± 0.01 nm/log(cells/mL) was obtained for three independent assays. An additional assay revealed that including molybdenum disulfide resulted in an increase of approximately 50% in sensitivity. Specificity and selectivity were also evaluated, and the sensors were used to analyze contaminated water samples, which verified their promising applicability in the aquaculture field.

Redox Homeostasis Disclosed in the Saltmarsh Plant Halimione portulacoides upon Short Waterborne Exposure to Inorganic Mercury.

The saltmarsh plant Halimione portulacoides was shortly exposed to realistic levels of inorganic mercury (iHg) with the aim of investigating the adaptative processes of the roots and leaves regarding redox homeostasis, physiology, and Hg accumulation. Plants were collected at a contaminated (CONT) and a reference (REF) site to address the interference of contamination backgrounds. The influence of major abiotic variables (i.e., temperature and light) was also examined. Total Hg levels, antioxidant enzymes, lipid peroxidation (LPO), and photosynthetic activity were analyzed after 2 and 4 h of exposure. A poor accumulation of Hg in the roots was noticed, and no translocation to the stems and leaves was found, but plants from the CONT site seemed more prone to iHg uptake (in winter). Despite this, antioxidant modulation in the roots and leaves was found, disclosing, in winter, higher thresholds for the induction of enzymatic antioxidants in CONT leaves compared to REF plants, denoting that the former are better prepared to cope with iHg redox pressure. Consistently, CONT leaves exposed to iHg had remarkably lower LPO levels. Exposure did not impair photosynthetic activity, pinpointing H. portulacoides' ability to cope with iHg toxicity under very-short-term exposure. Biochemical changes were noticed before enhancements in accumulation, reinforcing the relevance of these responses in precociously signaling iHg toxicity.

CONSTIPATION SCORING SYSTEM VALIDATED FOR THE PORTUGUESE LANGUAGE (ÍNDICE DE GRAVIDADE DA CONSTIPAÇÃO INTESTINAL): IS IT RELIABLE IN ASSESSING THE SEVERITY OF INTESTINAL CHRONIC CONSTIPATION IN OUR POPULATION?

BACKGROUND: There is a lack of valid and specific tools to measure chronic constipation severity in Brazil. AIMS: To validate the Constipation Scoring System for Brazilian spoken Portuguese. METHODS: Translation, cultural adaptation, and validation itself (reliability and convergent and divergent validation). Translation: definitive version from the original version's translation and evaluation by specialists. Cultural adaptation: score content analysis of the definitive version, as an interview to patients. Interobserver reliability: application by two researchers on the same day. Intraobserver reliability: same researcher at different times, in a 7-day interval. Divergent validation: non-constipated volunteers. Convergent validation: two groups, good response to clinical treatment and refractory to treatment. RESULTS: Cultural adaptation: 81 patients, 89% female, with mean age of 55 and seven years of schooling, and overall content validity index was 96.5%. Inter and intraobserver reliability analysis: 60 patients, 86.7% female, mean age of 56 and six years of schooling, and the respective intraclass correlation coefficients were 0.991 and 0.987, p<0.001. Divergent validation: 40 volunteers, 25 male, mean age of 49 years, and the mean global score was 2. Convergent validation of patients with good response to clinical treatment: 47 patients, 39 female, mean age of 60 and six years of schooling, and the pre- and post-treatment scores were 19 and 8, respectively (p<0.001). Convergent validation of refractory to clinical treatment patients: 75 patients, 70 female, mean age of 53 and seven years of schooling, and the global average score was 22. CONCLUSIONS: The Constipation Scoring System (Índice de Gravidade da Constipação Intestinal) validated for the Brazilian population is a reliable instrument for measuring the severity of intestinal chronic constipation.

Machine Learning-Based Prediction of Responsiveness to Neoadjuvant Chemoradiotheapy in Locally Advanced Rectal Cancer Patients from Endomicroscopy.

The protocol for treating locally advanced rectal cancer consists of the application of chemoradiotherapy (neoCRT) followed by surgical intervention. One issue for clinical oncologists is predicting the efficacy of neoCRT in order to adjust the dosage and avoid treatment toxicity in cases when surgery should be conducted promptly. Biomarkers may be used for this purpose along with in vivo cell-level images of the colorectal mucosa obtained by probe-based confocal laser endomicroscopy (pCLE) during colonoscopy. The aim of this article is to report our experience with Motiro, a computational framework that we developed for machine learning (ML) based analysis of pCLE videos for predicting neoCRT response in locally advanced rectal cancer patients. pCLE videos were collected from 47 patients who were diagnosed with locally advanced rectal cancer (T3/T4, or N+). The patients received neoCRT. Response to treatment by all patients was assessed by endoscopy along with biopsy and magnetic resonance imaging (MRI). Thirty-seven patients were classified as non-responsive to neoCRT because they presented a visible macroscopic neoplastic lesion, as confirmed by pCLE examination. Ten remaining patients were considered responsive to neoCRT because they presented lesions as a scar or small ulcer with negative biopsy, at post-treatment follow-up. Motiro was used for batch mode analysis of pCLE videos. It automatically characterized the tumoral region and its surroundings. That enabled classifying a patient as responsive or non-responsive to neoCRT based on pre-neoCRT pCLE videos. Motiro classified patients as responsive or non-responsive to neoCRT with an accuracy of ~ 0.62 when using images of the tumor. When using images of regions surrounding the tumor, it reached an accuracy of ~ 0.70. Feature analysis showed that spatial heterogeneity in fluorescence distribution within regions surrounding the tumor was the main contributor to predicting response to neoCRT. We developed a computational framework to predict response to neoCRT by locally advanced rectal cancer patients based on pCLE images acquired pre-neoCRT. We demonstrate that the analysis of the mucosa of the region surrounding the tumor provides stronger predictive power.

Negative lipid membranes enhance the adsorption of TAT-decorated elastin-like polypeptide micelles.

A cell-penetrating peptide (CPP) is a short amino-acid sequence capable of efficiently translocating across the cellular membrane of mammalian cells. However, the potential of CPPs as a delivery vector is hampered by the strong reduction of its translocation efficiency when it bears an attached molecular cargo. To overcome this problem, we used previously developed diblock copolymers of elastin-like polypeptides (ELPBCs), which we end functionalized with TAT (transactivator of transcription), an archetypal CPP built from a positively charged amino acid sequence of the HIV-1 virus. These ELPBCs self-assemble into micelles at a specific temperature and present the TAT peptide on their corona. These micelles can recover the lost membrane affinity of TAT and can trigger interactions with the membrane despite the presence of a molecular cargo. Herein, we study the influence of membrane surface charge on the adsorption of TAT-functionalized ELP micelles onto giant unilamellar vesicles (GUVs). We show that the TAT-ELPBC micelles show an increased binding constant toward negatively charged membranes compared to neutral membranes, but no translocation is observed. The affinity of the TAT-ELPBC micelles for the GUVs displays a stepwise dependence on the lipid charge of the GUV, which, to our knowledge, has not been reported previously for interactions between peptides and lipid membranes. By unveiling the key steps controlling the interaction of an archetypal CPP with lipid membranes, through regulation of the charge of the lipid bilayer, our results pave the way for a better design of delivery vectors based on CPPs.

LAPAROSCOPIC RIGHT AND LEFT COLECTOMY: WHICH PROVIDES BETTER POSTOPERATIVE RESULTS FOR ONCOLOGY PATIENTS?

BACKGROUND: The laparoscopic approach considerably reduced the morbidity of colorectal surgery when compared to the open approach. Among its benefits, we can highlight less intraoperative bleeding, early oral intake, lower rates of surgical site infection, incisional hernia, and postoperative pain, and earlier hospital discharge. AIMS: To compare the perioperative morbidity of right versus left colectomy for cancer and the quality of laparoscopic oncologic resection. METHODS: Retrospective analysis of patients submitted to laparoscopic right and left colctomy between 2006 and 2016. Postoperative complications were classified using the Clavien-Dindo scale, 30 days after surgery. RESULTS: A total of 293 patients were analyzed, 97 right colectomies (33.1%) and 196 left colectomies (66.9%). The averageage was 62.8 years. The groups were comparable in terms of age, comorbidities, body mass index, and the American Society of Anesthesiology (ASA) classification. Preoperative transfusion was higher in the right colectomy group (5.1% versus 0.4%, p=0.004, p<0.05). Overall, 233 patients (79.5%) had no complications. Complications found were grade I and II in 62 patients (21.1%) and grade III to V in 37 (12.6%). Twenty-three patients (7.8%) underwent reoperation. The comparison between left and right colectomy was not statistically different for operative time, conversion, reoperation, severe postoperative complications, and length of stay. The anastomotic leak rate was comparable in both groups(5.6% versus 2.1%, p=0.232, p>0.05). The oncological results were similar in both surgeries. In multiple logistic regression, ASA statistically influenced the worst results (≥ III; p=0.029, p<0.05). CONCLUSIONS: The surgical and oncological results of laparoscopic right and left colectomies are similar, making this the preferred approach for both procedures.

Do spirituality and emotional intelligence improve the perception of the ability to provide care at the end of life? The role of knowledge and self-efficacy.

OBJECTIVES: Spirituality, emotional intelligence, and palliative care (PC) knowledge have a positive and direct influence on self-efficacy and on perception of preparation and ability to provide end-of-life (EOL) care. The aim of this work is to propose a conceptual model that relates spirituality, emotional intelligence, PC knowledge, self-efficacy, and the preparation and ability to provide EOL care by doctors and nurses. METHODS: Quantitative, exploratory, descriptive, and inferential study applied to doctors and nurses in a hospital in the north of Portugal, between May and July 2022. The data collection instrument includes a questionnaire. The relationships between latent variables were evaluated using structural equation models by the partial least squares method using the Smart PLS 3.0 software. It was obtained the previous authorization of the ethics committee. RESULTS: The results (n = 380) indicate that self-efficacy, spirituality, and PC knowledge have a positive influence on the ability to provide EOL care. Emotional intelligence and spirituality have a direct and positive effect on self-efficacy. There is no direct influence of emotional intelligence on the ability to provide EOL care, but emotional intelligence has an indirect effect mediated by self-efficacy. SIGNIFICANCE OF RESULTS: Spirituality, self-efficacy, and emotional intelligence are very important for the ability of doctors and nurses to provide EOL care. The identification of predictive factors of the ability to provide EOL care and the determination of the relationship between them can improve the provision of EOL care, reduction of health costs, timely and early referral of people to PC, and increase life quality.