RESUMEN
La Lucha Canaria (LC) ha sido recientemente reconocida como deporte por el Consejo Superior de Deportes, aunque su origen se pierde en el tiempo. Sin embargo, no hay ningún trabajo en la bibliografía científica que describa qué ocurre fisiológicamente durante la competición en esta modalidad de lucha, y éste es el objetivo del presente trabajo. Con una muestra de 12 luchadores de alto nivel (puntales) simulamos el sistema de competición actual en Lucha Canaria. Se estudió la frecuencia cardiaca, la tensión arterial y la concentración de lactato en sangre en diferentes momentos de la simulación. Los patrones observados de respuesta de FC, TA y lactato, caracterizan a la competición en LC como un ejercicio intermitente, con picos de intensidad que superan el umbral láctico y determinan incrementos importantes de FC y TA sistólica. Este estudio es el primero en que se valora la respuesta fisiológica del luchador canario en competición (AU)
The aim of this article is to describe what happens from a physiological point of view in a Canarian Wrestling (CW) contest. The Spanish National Sports Council recognized CanarianWrestling as a sport on June 1st, 2009, although its origin is not very well documented. No scientific article exists in the scientific literature to describe what happens physiologically during a CW contest. Twelve high level wrestlers were recruited to simulate a CW match. The study involved data about heart rate (HR), blood pressure (BP) and blood lactate levels (L) at different moments of the combat. The conclusions of the data observed concerning HR, BP and L classify Canarian Wrestling as an intermittent exercise, with intensity peaks that exceed the lactic threshold and high levels of HR and systolic BP. This is the first paper in which the physiological responses in a Canarian Wrestling competition are reported (AU)
Asunto(s)
Humanos , Lucha/fisiología , Deportes/fisiología , Ácido Láctico/sangre , Frecuencia Cardíaca/fisiología , Presión Arterial/fisiología , SomatotiposRESUMEN
Subcommissural organ (SCO) secretory activity of the goat (variations of Capra hircus, that live in arid conditions) was examined during the postnatal development, using specific antibodies against the Reissner's fibre (AFRU) and angiotensin II (AAGII). The SCO was strongly stained with the anti-glycoproteins that form the Reissner's fibre and lightly marked with the anti-angiotensin II. The AFRU-immunoreactivity (ir) was found in the ependymal and hypendymal cells and in the ventricular and peripheral secretory routes of the goat SCO. The amount AFRU increases at 6 months and decreases at adult age. In contrast, the anti-angiotensin II-ir was mainly found in the adult age, not being practically observed at one postnatal month. The AAGII-ir was mainly found in ependymal cells in which AFRU-ir was downregulated. In addition, we detected the presence of double immunostained for AFRU and AAGII in ependymocytes of the pre-commissural and subcommissural parts. In conclusion the present results may suggest a functional interrelation between AAGII and the secretory activity of the SCO of this kind of goat.
Asunto(s)
Angiotensina II/análisis , Fibras Nerviosas/inmunología , Órgano Subcomisural/citología , Órgano Subcomisural/metabolismo , Envejecimiento/fisiología , Angiotensina II/inmunología , Animales , Glicoproteínas/inmunología , Cabras , Inmunohistoquímica/veterinaria , Órgano Subcomisural/crecimiento & desarrolloRESUMEN
We studied the effects of spontaneous high blood pressure and the captopril treatment on the subfornical organ (SFO) of rats. The brains of control Wistar-Kyoto rats (WKY), WKY rats treated with captopril (WKY-T), spontaneously hypertensive rats (SHR) and SHR rats treated with captopril (SHR-T) were processed immunohistochemically using anti-angiotensin II as primary antibody. Immunorective material (IRM) for angiotensin II was observed in a group of neurons and some cells of the ependymal layer of the SFO in WKY rats. The angiotensin II immunoreactive (AGII-ir) in the SHR rats was decreased, showing positive reaction only in a few neurons, while captopril treatment induced an increase in immunoreactive material in hypertensive rats, but contrarily, the expression of AGII-ir in the WKY-T group was scarce. The variations of the angiotensin II observed in the SFO could be owing to an interaction between the hypertension and its captopril treatment.
Asunto(s)
Angiotensina II/metabolismo , Antihipertensivos/farmacología , Captopril/farmacología , Órgano Subfornical/efectos de los fármacos , Animales , Citoplasma/efectos de los fármacos , Citoplasma/metabolismo , Inmunohistoquímica , Masculino , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Ratas , Ratas Endogámicas SHR , Órgano Subfornical/metabolismoRESUMEN
La mayoría de las modalidades de lucha clasifican a los practicantes agrupándolos por peso. En Lucha Canaria los mejores luchadores son denominados "puntales" (A, B y C), sin considerar sus pesos. Existen pocos trabajos morfológicos sobre luchadores de la modalidad canaria, lo que supone una falta de información científica que podría limitar su rendimiento. No existe un Somatotipo de Referencia (SR) para éstos luchadores, de ahí que el principal objetivo de este trabajo sea establecer el citado somatotipo. Se han medido doscientos diecinueve (219) luchadores siguiendo las directrices de la International Society for the Advancement of Kinanthropometry (ISAK) y el Grupo Español de Cincantropometría (GREC). Con ellos se hicieron cuatro grupos (A, B, C y NC) atendiendo a la clasificación establecida por su rendimiento en competición. El grupo A lo constituyeron 5 luchadores puntales A, que es la categoría más alta en este deporte. El grupo B lo fonnaron 9 luchadores puntales B, el grupo C 28 luchadores puntales C y, finalmente, los luchadores No Clasificados (grupo NC) que fueron 177 individuos tomados al azar. Estos últimos fueron además divididos en 5 subgrupos atendiendo a su peso: 60K, 70K, 8OK, 90K y mas de 1OOK.El SR se calculó utilizando el Método Antropométrico de Heath- Carter. Nuestro estudio puso de manifiesto que los luchadores más viejos eran los puntales B, y los no clasificados los más jóvenes. Los puntales A eran los más altos y los más pesados. Todos ellos fueron endo-mesomorfos, con la excepción de¡ subgrupo 60K de no clasificados (ecto-mesomorfo). Globalmente, el componente mesomorfo fue dominante, y la endomorfj'a mayor que la ectomorfia. Podemos concluir que, sin considerar el éxito en competición, la fonna actual de clasificación otorga el rango de punta¡ a aquellos luchadores con un somatotipo de (5.9-8.9-0. l). En ambos casos, puntales y no clasificados, la distribución de los somatotipos según el componente dominante reveló no ser muy similares, como se desprende del SDD y de¡ SAD. Sin embargo, se requieren nuevos estudios para detemiinar el SR de los no clasificados (AU)
Asunto(s)
Adulto , Masculino , Humanos , Lucha/fisiología , Somatotipos/fisiología , Peso Corporal/fisiología , Deportes/fisiología , Distribución por EdadRESUMEN
We studied immunohistochemically the posterior lobe of the hypophysis (PL) of 15-week-old spontaneously hypertensive rats (SHR) and of matched normotensive Wistar Kyoto rats (WKY), by using our own polyclonal antibody raised in mice against Angiotensin II (mouse-antiangiotensin II, MAAII). The blood pressure, water intake and volume of the PL were also recorded. The SHR rats were hypertensive, drank more water and showed a clear hypertrophy of their hypophysial PL. Also the PL of the SHR animals showed an increase in the immunoreactivity to the anti-angiotensin II antibody in the fibres arriving at the PL, with respect to the PL of WKY rats. This increase is compatible with the hyperactivity of the brain RAS, depletion of vasopressin content in the PL and increase in plasmatic levels of vasopressin described in SHR rats with respect to normotensive animals, as angiotensin II could locally stimulate vasopressin release to plasma from the neurohypophysis.
Asunto(s)
Angiotensina II/análisis , Hipertensión/patología , Fibras Nerviosas/patología , Neurohipófisis/patología , Animales , Anticuerpos , Inmunohistoquímica/métodos , Masculino , Ratones , Adenohipófisis/patología , Ratas , Ratas Endogámicas SHR , Ratas Endogámicas WKYRESUMEN
The present study analyses the effects of hypertension and/or its oral treatment with captopril (angiotensine-converting enzyme inhibitor) on the rat median eminence (ME) and the posterior lobe of the hypophysis (PL). After an immunohistochemical reaction using an antibody against arginine-vasopressin, we compared by densitometry the amount of vasopressin immunoreactive material (vasopressin-ir) of these centers in 4 groups of animals: control Wistar Kyoto rats (WKY), spontaneously hypertensive rats (SHR), WKY rats treated with captopril (WKY-T) and SHR rats also treated with the same drug (SHR-T). Captopril was administrated at a dosage of 0.1 mg/ml in the drinking water from the 8th to the 15th weeks. We have found that the rats showing the lowest level of vasopressin-ir, in both ME and PL, were those from the SHR group, the concentration increasing after oral captopril treatment (SHR-T), although without reaching the values of WKY rats. Then, ACE inhibition by captopril influences vasopressin content in brain areas where the hormone is concentrated before being released, which supports the hypothesis that suggests a central modulatory effect of ACE inhibitors, contributing to their therapeutic action on hypertension.
Asunto(s)
Inhibidores de la Enzima Convertidora de Angiotensina/farmacología , Arginina Vasopresina/análisis , Captopril/farmacología , Hipertensión/metabolismo , Eminencia Media/química , Neurohipófisis/química , Inhibidores de la Enzima Convertidora de Angiotensina/administración & dosificación , Animales , Captopril/administración & dosificación , Densitometría , Ingestión de Líquidos , Hipertensión/patología , Técnicas para Inmunoenzimas , Masculino , Eminencia Media/patología , Neurohipófisis/patología , Ratas , Ratas Endogámicas SHR , Ratas Endogámicas WKYRESUMEN
The subcommissural organ (SCO) is a glandular circumventricular organ secreting glycoproteins into the cerebrospinal fluid. The SCO of 15-week-old spontaneously hypertensive rats (SHR) and of matched normotensive Wistar-Kyoto rats (WKY) was studied immunocytochemically by using an antibody against the glycoproteins secreted by the SCO. The blood pressure, water intake and volume of brain ventricles of SHR and WKY rats were also recorded. The SHR were hypertensive, drank more water and did not display dilatation of the brain ventricles. The SCO of the SHR rats showed a drastic decrease of the immunoreactive material stored in the rough endoplasmic reticulum whereas the amount of immunoreactive apical secretory granules did not vary with respect to the SCO of WKY rats. These changes are compatible with an increased secretory activity of the SCO of the SHR rats. It is suggested that the changes in the SCO of SHR rats, and their hypertensive state, are interrelated phenomena.
Asunto(s)
Órgano Subcomisural/metabolismo , Órgano Subcomisural/fisiología , Animales , Anticuerpos/metabolismo , Presión Sanguínea/fisiología , Ingestión de Líquidos/fisiología , Glicoproteínas/inmunología , Hipertensión/etiología , Inmunohistoquímica , Masculino , Ratas , Ratas Endogámicas SHR , Ratas Endogámicas WKY , Cloruro de Sodio/metabolismo , Órgano Subcomisural/citología , Equilibrio Hidroelectrolítico/fisiologíaRESUMEN
We have studied the morphometric changes of the neurons of the cingular area 24 and frontal area 6 of the mouse, produced by age and/or chronic alcohol intake. The parameters analyzed were nuclear area of these cortical neurons and cellular density (cell/neuropil coefficient). We detected a decrease in the number of neurons with age in practically all layers of the control animals. In the animals that chronically ingested the alcoholic solution, we also detected a decrease in the number of neurons with age, but only in layer V of the frontal cortex and in layer VI of the cingular area 24. The comparison between the control and the alcoholic group showed that alcohol intake caused an increase in the nuclear area of the neurons in layer II-III of the frontal cortex at 180 days, while in the cingular cortex the increase in nuclear area of its neurons was significative at 180 days in layer II-III and at 35 and 180 days in layers V and VI. We think that these changes are the expression of the neuronal plasticity in both cortical areas in response to the alcohol exposure.
Asunto(s)
Envejecimiento/efectos de los fármacos , Alcoholismo/patología , Alcoholes/toxicidad , Corteza Cerebral/efectos de los fármacos , Lóbulo Frontal/efectos de los fármacos , Neuronas/efectos de los fármacos , Consumo de Bebidas Alcohólicas/efectos adversos , Animales , Peso Corporal , Corteza Cerebral/patología , Lóbulo Frontal/patología , Ratones , Actividad MotoraRESUMEN
The effects of Captopril (an angiotensin-converting enzyme inhibitor) on the median eminence (ME) of the male albino mouse have been examined using morphometric and immunohistochemical procedures. We measured the nuclear area of the ependymocytes of the ME and of the glial cells of the reticular external zone of the ME. We also determined the cell/neuropil coefficient (CNC), which expresses the relation between cellular area and neuropil of the ME, and the global volume of the ME in each animal. For the immunohistochemical study we used rabbit antiarginine-vasopressin, and compared the results in the different groups of mice. We detected an increased in the immunoreactive material (arginine-vasopressin, A-V) and an increase in the global volume of the organ and also an increase of the neuropil of the ME after the longest exposure to the drug. These alterations could be related to the inhibition of the brain angiotensin II by captopril and the accumulation of vasopressin in the fibrous tract that runs from the paraventricular nucleus (PVN) to the neurohypophysis.
Asunto(s)
Inhibidores de la Enzima Convertidora de Angiotensina/administración & dosificación , Captopril/administración & dosificación , Eminencia Media/efectos de los fármacos , Administración Oral , Animales , Arginina/metabolismo , Núcleo Celular/efectos de los fármacos , Núcleo Celular/metabolismo , Esquema de Medicación , Epéndimo/efectos de los fármacos , Epéndimo/metabolismo , Epéndimo/ultraestructura , Técnicas para Inmunoenzimas , Masculino , Eminencia Media/metabolismo , Eminencia Media/ultraestructura , Ratones , Neuroglía/efectos de los fármacos , Neuroglía/metabolismo , Conejos , Vasopresinas/metabolismoRESUMEN
The present study analyses the response of the paraventricular (PVN) and supraoptic (SPO) nuclei of the hypothalamus of the male mouse to chronic alcohol intake by immunohistochemical and morphometric methods. We relate the intensity of the reaction to A-V with the vasopressin content of the nucleus, as all the slides, from the control and experimental groups, were processed at the same time and with the same solutions of the antibodies. We suggest that the accumulation of vasopressin, observed in the alcohol-treated animals, of both hypothalamic nuclei could be related to an inhibition of vasopressin release and/or transport from the SPO and PVN to the neurohypophysis and to an increase in vasopressin synthesis in the SPO as this nucleus shows an increase in its nuclear sizes, an index of the function of the neurons.
Asunto(s)
Alcoholismo/patología , Arginina Vasopresina/metabolismo , Núcleo Hipotalámico Paraventricular/patología , Núcleo Supraóptico/patología , Animales , Procesamiento de Imagen Asistido por Computador , Técnicas para Inmunoenzimas , Masculino , RatonesRESUMEN
We have analyzed the morphometric effects on the dorsal vagal complex (DVC) of the rat of alcohol exposure and/or hypoproteic diet intake during 8 weeks. In the area postrema (AP), alcohol treatment (combined with normal isoproteic or hypoproteic diet) caused a significant decrease in karyometric parameters. In the dorsal motor nucleus of the vagus (DMV) and nucleus tractus solitari (NTS), the alcohol isoproteic intake (AI) produced an increase in neuron size (expressed by an increase in the neuronal nuclear area and the cell/neuropil coefficient). The hypoproteic diets produced a reduction in the global volume of each structure of the DVC which was accompanied by a decrease in global brain volume. These results indicate that after 8 weeks of treatment, alcohol is the main cause of the morphometric alteration found in the DVC, while variations in the amount of protein intake appear to produce global effects on the whole brain.