RESUMEN
The ability of Halomonas maura to bioprecipitate carbonate and sulphate crystals in solid media at different manganese concentrations has been demonstrated in this study for the first time. The precipitated minerals were studied by X-ray diffraction, scanning and transmission electron microscopy, and energy-dispersive X-ray spectroscopy. The precipitated minerals were different based on the manganese concentration present in the medium and the incubation time. In the absence of manganese, H. maura formed pseudokutnahorite crystals; in the presence of manganese, the concentration in the culture medium determined the precipitation carbonates, such as rhodochrosite and dolomites. However, in the presence of low concentrations of manganese chloride (MnCl2) (5 g/l), kutnohorite crystals were also formed. Finally, when H. maura was grown in the presence of manganese, small amounts of sulphate crystals (such as bassanite and gypsum) were detected. Our study of the precipitated minerals showed an active role of H. maura in the biomineralisation process, but the geochemical conditions, and the manganese concentrations in particular, were clearly influential.
Asunto(s)
Carbonatos/metabolismo , Halomonas/metabolismo , Compuestos de Manganeso/metabolismo , Sulfatos/metabolismo , Carbonatos/química , Cristalización , Sedimentos Geológicos/química , Sedimentos Geológicos/microbiología , Halomonas/ultraestructura , Compuestos de Manganeso/química , Sulfatos/químicaRESUMEN
A bench-scale granular autotrophic nitrogen removal bioreactor (completely autotrophic nitrogen removal over nitrite (CANON) system) used for the treatment of synthetic wastewater was analyzed for the identification of microbiota with potential capacity for carbonate and phosphate biomineral formation. 16S ribosomal RNA (rRNA) gene-based studies revealed that different bacterial species found in the granular biomass could trigger the formation of phosphate and calcite minerals in the CANON bioreactor. iTag analysis of the microbial community in the granular biomass with potential ability to precipitate calcium carbonate and hydroxyapatite constituted around 0.79-1.32 % of total bacteria. Specifically, the possible hydroxyapatite-producing Candidatus Accumulibacter had a relative abundance of 0.36-0.38 % and was the highest phosphate-precipitating bacteria in the granular CANON system. With respect to calcite precipitation, the major potential producer was thought to be Stenotrophomonas with a 0.38-0.50 % relative abundance. In conclusion, our study showed evidences that the formation of hydroxyapatite and calcite crystals inside of the granular biomass of a CANON system for the treatment wastewater with high ammonium concentration was a biological process. Therefore, it could be suggested that microorganisms play an important role as a precipitation core and also modified the environment due to their metabolic activities.
Asunto(s)
Bacterias/metabolismo , Reactores Biológicos/microbiología , Carbonatos/metabolismo , Precipitación Química , Nitrógeno/metabolismo , Fosfatos/metabolismo , Bacterias/clasificación , Bacterias/genética , Bacterias/aislamiento & purificación , Análisis por Conglomerados , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Filogenia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Aguas Residuales/microbiología , Purificación del Agua/métodosRESUMEN
The ability of bacteria isolated from a fixed-film bioreactor to precipitate phosphate crystals for the treatment of domestic wastewater in both artificial and natural media was studied. When this was demonstrated in artificial solid media for crystal formation, precipitation took place rapidly, and crystal formation began 3 days after inoculation. The percentage of phosphate-forming bacteria was slightly higher than 75%. Twelve major colonies with phosphate precipitation capacity were the dominant heterotrophic platable bacteria growing aerobically in artificial media. According to their taxonomic affiliations (based on partial sequencing of the 16S rRNA), the 12 strains belonged to the following genera of Gram-negative bacteria: Rhodobacter, Pseudoxanthobacter, Escherichia, Alcaligenes, Roseobacter, Ochrobactrum, Agromyce, Sphingomonas and Paracoccus. The phylogenetic tree shows that most of the identified populations were evolutionarily related to the Alphaproteobacteria (91.66% of sequences). The minerals formed were studied by X-ray diffraction, scanning electron microscopy (SEM), and energy dispersive X-ray microanalysis (EDX). All of these strains formed phosphate crystals and precipitated struvite (MgNH4PO4·6H2O), bobierrite [Mg3(PO4)2·8H2O] and baricite [(MgFe)3(PO4)2·8H2O]. The results obtained in this study show that struvite and spherulite crystals did not show any cell marks. Moreover, phosphate precipitation was observed in the bacterial mass but also near the colonies. Our results suggest that the microbial population contributed to phosphate precipitation by changing the media as a consequence of their metabolic activity. Moreover, the results of this research suggest that bacteria play an active role in the mineral precipitation of soluble phosphate from urban wastewater in submerged fixed-film bioreactors.
Asunto(s)
Bacterias/metabolismo , Reactores Biológicos/microbiología , Minerales/química , Fosfatos/química , Eliminación de Residuos Líquidos/métodos , Bacterias/clasificación , Bacterias/genética , Biopelículas , Precipitación QuímicaRESUMEN
The production of Mg-rich carbonates by Idiomarina bacteria at modern seawater salinities has been investigated. With this objective, four strains: Idiomarina abyssalis (strain ATCC BAA-312), Idiomarina baltica (strain DSM 15154), Idiomarina loihiensis (strains DSM 15497 and MAH1) were used. The strain I. loihiensis MAH1 is a new isolate, identified in the scope of this work. The four moderately halophilic strains precipitated struvite (NH4MgPO4 x 6H2O) crystals that appear encased by small Ca-Mg kutnahorite [CaMg(CO3)2] spheres and dumbbells, which are also regularly distributed in the bacterial colonies. The proportion of Ca-Mg kutnahorite produced by the bacteria assayed ranged from 50% to 20%, and I. abyssalis also produced monohydrocalcite. All precipitated minerals appeared to be related to the bacterial metabolism and, consequently, can be considered biologically induced. Amino acid metabolism resulted in a release of ammonia and CO2 that increase the pH and CO(3)(2-) concentration of the culture medium, creating an alkaline environment that favoured carbonate and struvite precipitation. This precipitation may be also related to heterogeneous nucleation on negatively charged points of biological structures. Because the nature of the organic matrix determines which ion is preferentially adsorbed and, consequently, which mineral phase is formed, the uniquely high content in odd-iso-branched fatty acids of the Idiomarina suggests that their particular membrane characteristics could induce Ca-Mg kutnahorite production. The Ca-Mg kutnahorite, a mineral with a dolomite-ordered structure, production at seawater salinities is noticeable. To date, such precipitation in laboratory cultures, has only been described in hypersaline conditions. It has also been the first time that biomineralization processes have been related to Idiomarina bacteria.
Asunto(s)
Alteromonadaceae/metabolismo , Compuestos de Magnesio/metabolismo , Fosfatos/metabolismo , Agua de Mar/microbiología , Alteromonadaceae/clasificación , Alteromonadaceae/ultraestructura , Amoníaco/metabolismo , Dióxido de Carbono/metabolismo , Compuestos de Magnesio/química , Microscopía Electrónica de Rastreo , Microscopía Electrónica de Transmisión , Fosfatos/química , Filogenia , Protones , ARN Ribosómico 16S/genética , Salinidad , Agua de Mar/química , EstruvitaRESUMEN
An experimental and theoretical investigation on crystal forms of the popular and ubiquitous pharmaceutical Minoxidil is presented here. A new crystallization method is presented for Minoxidil (6-(1-piperidinyl)-2,4-pyrimidinediamide 3-oxide) in ethanol-poly(ethylene glycol), yielding crystals with good quality. The crystal structure is determined at low temperature, with a final R value of 0.035, corresponding to space group P2(1) (monoclinic) with cell dimensions a = 9.357(1) A, b = 8.231(1) A, c = 12.931(2) A, and beta = 90.353(4) degrees . Theoretical calculations of the molecular structure of Minoxidil are set forward using empirical force fields and quantum-mechanical methods. A theoretical prediction for Minoxidil crystal structure shows many possible polymorphs. The predicted crystal structures are compared with X-ray experimental data obtained in our laboratory, and the experimental crystal form is found to be one of the lowest energy polymorphs.