RESUMEN
Multipotent mesenchymal stromal cells (MSCs) have been described as bone marrow stromal cells, which can form cartilage, bone or hematopoietic supportive stroma. In 2006, the International Society for Cell Therapy (ISCT) established a set of minimal characteristics to define MSCs. According to their criteria, these cells must express CD73, CD90 and CD105 surface markers; however, it is now known they do not represent true stemness epitopes. The objective of the present work was to determine the surface markers for human MSCs associated with skeletal tissue reported in the literature (1994-2021). To this end, we performed a scoping review for hMSCs in axial and appendicular skeleton. Our findings determined the most widely used markers were CD105 (82.9%), CD90 (75.0%) and CD73 (52.0%) for studies performed in vitro as proposed by the ISCT, followed by CD44 (42.1%), CD166 (30.9%), CD29 (27.6%), STRO-1 (17.7%), CD146 (15.1%) and CD271 (7.9%) in bone marrow and cartilage. On the other hand, only 4% of the articles evaluated in situ cell surface markers. Even though most studies use the ISCT criteria, most publications in adult tissues don't evaluate the characteristics that establish a stem cell (self-renewal and differentiation), which will be necessary to distinguish between a stem cell and progenitor populations. Collectively, MSCs require further understanding of their characteristics if they are intended for clinical use.
RESUMEN
Objetivo: describir el estado de salud bucal en nativos Maká de 12 a 15 años de edad que residen en la ciudad de Mariano Roque Alonso, Paraguay. Materiales y métodos: Estudio observacional descriptivo de corte transversal en 59 niños de 12 a 15 años, evaluados por medio de la inspección oral y la aplicación de un cuestionario para indagar aspectos relativos a factores de riesgo presentes. Resultados: El Índice CPO-D fue de 10.5, se pudo constatar la ausencia del componente de piezas obturadas. El 94.9% presentó gingivitis leve. Se halló una prevalencia de 30.5% de Fluorosis y lesiones no cariosas en un 11% de la población examinada. Conclusión: los resultados de los índices obtenidos para medir las condiciones bucales se encuentran por encima de la meta establecida por la OMS; demuestrando un importante deterioro en el estado bucal de los niños nativos Maká.
Objetivo: Descrever a condição de saúde oral em nativos Maká entre 12 a 15 anos de idade residentes em Mariano Roque Alonso, Paraguai. Materiais e métodos: Estudo observacional descritivo de corte transversal em 59 crianças de 12 a 15 anos, avaliadas através do exame clínico e aplicação de um questionário para investigar questões relacionadas com fatores de risco. Resultados: O Índice CPO-D foi de 10.5, pôde-se constatar a ausência do componente de peças obturadas. O 94.9% apresentou gengivite leve. Foi encontrada uma prevalência de 30.5% de Fluorose e lesões não cariosas em 11% da população examinada. Conclusão: Os resultados obtidos, acima da meta estabelecida pela OMS; mostram uma deterioração significativa das condições de saúde bucal das crianças.
Aims: Describe the state of oral health in native Maka from 12 to 15 years old, Mariano Roque Alonso, Paraguay. Methods: Descriptive observational cross-sectional study in 59 children between 12 and 15 years old, evaluated through oral inspection and application of a questionnaire to investigate aspects of risk factors present. Results: The DMFT index was 10.5, this confirming the absence of sealed tooth, and 94.9 % presented mild gingivitis. A prevalence of 30.5 % of fluorosis and non-carious lesions in 11% of the population examined was found. Conclusion: The results showed a significant deterioration in the oral status of the Maka children due to the increased prevalence and higher caries index compared toa the goal set by WHO.