RESUMEN
Fibromyalgia (FM) is a complex pathology described as persistent hyperalgesia including somatic and mood dysfunctions, depression and anxiety. Although the etiology of FM is still unknown, a significant decrease in biogenic amines is a common characteristic in its pathogenesis. Here, our main objective was to investigate the role of dopamine D3/D2 receptor during the reserpine-induced pain in mice. Our results showed that pramipexole (PPX) - a dopaminergic D3/D2 receptor agonist - inhibited mechanical allodynia and thermal sensitivity induced by reserpine. Relevantly, PPX treatment decreased immobility time and increased the number of grooming in the forced swimming test and splash test, respectively. Animals that received PPX remained longer in the open arms than the reserpine group using elevated plus-maze apparatus. The repeated PPX administration, given daily for 4 days, significantly blocked the mechanical and thermal allodynia during FM model, similarly to pregabalin, although it failed to affect the reserpine-induced thermal nociception. Reserpine administration induced significant downregulation of dopamine concentration in the central nervous system, and repeated treatment with PPX restored dopamine levels in the frontal cortex and spinal cord tissues. Moreover, PPX treatment inhibited oxidants production such as DCFH (2',7'-dichlorodihydrofluorescein) and nitrite, also decreased oxidative damage (carbonyl), and upregulated the activity of superoxide dismutase in the spinal cord. Together, our findings demonstrated the ability of dopamine D3/D2 receptor-preferring agonist in reducing pain and mood dysfunction allied to FM in mice. All experimental protocols were approved by the Universidade Federal de Santa Catarina (UFSC) Ethics Committee (approval No. 2572210218) on May 10, 2018.
RESUMEN
A utilização de plantas medicinais para tratamento, cura e prevenção de doenças é uma das mais antigas formas de prática medicinal da humanidade. De acordo com as perspectivas da modernidade no contexto contemporâneo, a saúde do futuro estará voltada para a medicina preventiva, onde a ciência buscará, na natureza, meios profiláticos que auxiliem o homem na defesa de seus males. Essa pesquisa visa solucionar o uso do extrato liofilizado do fedegoso (Heliotropium indicum L.) pela população como antimicrobiano na cura de doenças dermatológicas (micoses) sem os conhecimentos fitoterápicos da espécie. Por isso, esta pesquisa tem como objetivo caracterizar os marcadores químicos presentes na tintura obtida do Heliotropium indicum L. avaliando o potencial antimicrobiano da espécie em estudo. As análises fitoquímicas e microbiológicas foram realizadas nos laboratórios de Bioquímica. O perfil químico indicou a presença de metabólitos secundários. Já a avaliação do potencial biológico da espécie em estudo apresentou inibição das cepas de Escherichia coli (ATCC 8739) e inibiu fungos Aspergillus fumigatus.(AU)
The use of medicinal plants for treatment, cure and prevention of diseases is the most ancient medical practice of humanity. According with the modernity perspectives in contemporary context, the future's health is going to be focus for the preventive medicine, where science will find in nature prophylactics ways for helping humandefend your life. This search is about the solution of using lyophilized extract from seaside heliotrope (Heliotropium indicum L.) for the population as an antimicrobial in curing dermatological diseases (mycoses) without the knowledge of the phytotherapics effects about the specie studied. Therefore, this search has as objective to characterize chemical markers presents in the dye obtained from Heliotropium indicum L., evaluating antimicrobial potential of the specie under study. The phytochemical and microbiological analyzes were performed in the laboratories of Biochemistry and Microscopy of the College of Southern Maranhão. The chemical profile indicated the presence of secondary metabolites. While the evaluation of the biological potential of the species studied showed inhibition of the strains of Escherichia coli (ATCC 8739) and inhibited fungus Aspergillus fumigatus.(AU)
