Comparative characteristics of the stem cells' number in the stromal vascular fraction of infrapatellar fat pad and subcutaneous fat tissue.

OBJECTIVES: The use of infrapatellar fat pad adipose stem cells (IPFP-ASCs) shows an age-independent proliferation and differentiation potential. In addition, the pronounced chondrogenic potential of IPFP-ASCs makes them promising candidates for research for use in other methods of regenerative therapy. The purpose of this study was to ascertain the presence and compare the relative abundance of cells exhibiting an immunohistochemical profile characteristic of adipose-derived mesenchymal stem cells in selected samples of the stromal vascular fraction (SVF) obtained from the IPFP and subcutaneous fat tissue. METHODS: A direct immunohistochemical study was carried out in serial paraffin sections of the SVF of the infrapatellar fat pad (IPFP) and subcutaneous tissue, using monoclonal antibodies. The minimum criteria were established by the International Society for Cell Therapy to ensure the identity of mesenchymal stem cells use CD73, CD90, and CD105 as positive markers and CD34, CD31, and CD45 as a negative. RESULTS: According to the results of histological, immunohistochemical, morphometric, and statistical studies, it was found that in the SVF of IPFP and subcutaneous adipose tissue, the relative number of cells with the profile CD105+, CD73+, CD34+, CD31-, CD45- in the standard field of view (×200), the SVF of IPFP was 1.58%, whereas the SVF of subcutaneous adipose tissue was 6.92 %, which was statistically significantly greater by 4.38 times (p â€‹< â€‹0.05). CONCLUSION: The presence of a sufficient number of mesenchymal stromal cells in IPFP in combination with their topographic relationship with the structures of the joint determines the use of the SVF of the IPFP for the treatment of diseases of the knee joint. LEVEL OF EVIDENCE: III.

SVF Therapy of Delayed Fracture Union in Patients with Multiple Combat Injury. Case Report.

Delayed union of fractures is one of the most frequent complications in orthopedic practice, especially in polytrauma patients. With the development of new methods of regenerative medicine, including the use of adipose derived stromal cells as a component of the stromal-vascular fraction (SVF), new possibilities for conservative treatment of this problem have emerged. This article presents a clinical case of conservative treatment of delayed union of a radial bone fracture using local SVF injections. In the fracture space, SVF with PRP creates a pool of cells that could differentiate towards surrounding tissue, releases various inducers of tissue growth and, via an indirect chemotactic effect on receptors, mobilizes the body's own resources and creates conditions for angiogenesis and trophism in the injured segment. In the patient with delayed consolidation after SFV-therapy, progress in clinical and radiological dynamics was noted with complete healing within 7 months. The positive clinical result provides a basis for further study and implementation in practice.

Biomechanical Substantiation of the Strength and Stiffness of a Hip Joint Capsule Defect Fixation with Polypropylene Mesh.

Formulation of the problem Dislocation of the femoral component of the endoprosthesis is one of the most frequent complications of total hip replacement. The best option for the "treatment" of dislocation of the hip endoprosthesis is to prevent the development of primary instability. There are cases in which even with the correct installation of the endoprosthesis components, dislocations arise due to the weakness or defect of the capsular-ligament apparatus. Currently, many methods have been developed to strengthen and restore the posterior structures of the capsule of the hip joint with the help of auto- and allomaterials, which differ in both the fixation technique and the characteristics of the materials themselves. In this paper, we propose a method for restoring and strengthening the posterior structures of the capsule of the hip joint using polypropylene-based graft implants. The purpose of this study is to, with the help of specialized software, build a model of the capsule of the hip joint after capsulotomy and to determine the stiffness capabilities of the defect covered with polypropylene mesh. Results The study was performed using a software package based on the finite-element method. As a result of the performed calculations, pictures of the distribution of the stress-strain state in the "head-capsule" system were obtained. To assess the effectiveness of the method of closing the capsule, from the viewpoint of rigidity, as the main characteristics, the values of the opening of the cut are selected. Conclusions Under the kinematic loading of the model, the smallest values of the opening of the section are obtained when it is closed by a grid. In the case of thread fixation, the values were higher by 8.5%. However, the values of equivalent stresses, both in the capsule and in the head, in the model with the grid turned out to be the largest. These stresses were higher by 23.8% in the capsule and by 60.4% in the head than the same values for the thread fixation model. The obtained results indicate that the model with a grid is more rigid in the considered fixation variants.