Using social cyberloafing to cope with workplace ostracism.

In this Journal Club, Natalie Mason describes a paper that finds a relationship between time spent on the internet for personal reasons and ostracism in the workplace.

Global opportunities and challenges for transboundary conservation.

Rapid biodiversity loss has prompted global action to prevent further declines, yet coordinated conservation action among nations remains elusive. As a result, species with ranges that span international borders-which include 53.8% of terrestrial birds, mammals and amphibians-are in increasing peril through uncoordinated management and artificial barriers to human movement, such as border fences. Transboundary conservation initiatives represent a unique opportunity to better protect species through coordinated management across national borders. Using metrics of governance, collaboration and human pressure, we provide an index of transboundary conservation feasibility to assess global opportunities and challenges for different nations. While the transboundary conservation potential of securing multinational threatened species varied substantially, there are distinct opportunities in South-East Asia, Northern Europe, North America and South America. But to successfully avert the loss of transboundary species, the global community must be prepared to invest in some regions facing greater implementation challenges, including the nations of Central Africa, where efforts may necessitate establishing rapid conservation interventions postconflict that align with local socio-cultural opportunities and constraints. Sanctioned and coordinated approaches towards managing transboundary species are now essential to prevent further declines of many endangered species, and global policy efforts must do more to produce and enact legitimate mechanisms for collaborative action in conservation.

Virtual Visits for Upper Respiratory Tract Infections in Adults Associated with Positive Outcome in a Cox Model.

There is no previous work on the relationship between a virtual visit for viral upper respiratory tract infection and improved outcome, even though there is data on the prevalence and other descriptors. We do not know if a virtual visit is an independent prognostic factor in community-based patients. With the exponential growth of this type of clinical visit, it is important for both clinical and planning considerations to evaluate this question. We analyzed a cohort of adult patients with newly diagnosed viral upper respiratory tract infection from a database of health plan patients seen virtually on telemedicine and in person at urgent cares in Las Vegas, Nevada between January 2014 and September 2014. Logistic regression, Kaplan-Meier survival analysis, and Cox proportional hazard model were used. Among the final 6,756 patients selected with upper respiratory tract infections (median age of 41.5), 6% had virtual visits, while the rest were seen in person at urgent cares. Patients who had virtual visits were more likely to be younger, but had no other firm demographic differences from those seen for upper respiratory tract infections in urgent care. Hazard ratio for 2-week follow-up (= failure), with no significant effect from covariates, was 0.55 (confidence interval 0.324-0.939, p < 0.05) in virtual patients. In this cohort of patients with upper respiratory tract infection, a virtual visit, compared to an in-person one at urgent care, is an independent prognostic factor for less follow-up within 2 weeks. Further research into other age groups, time periods, and different diagnoses using similar methodology is warranted.

MicroRNA-510 promotes cell and tumor growth by targeting peroxiredoxin1 in breast cancer.

INTRODUCTION: MicroRNAs are small non-coding RNAs that are involved in the post-transcriptional negative regulation of mRNAs. MicroRNA 510 (miR-510) was initially shown to have a potential oncogenic role in breast cancer by the observation of its elevated levels in human breast tumor samples when compared to matched non-tumor samples. Few targets have been identified for miR-510. However, as microRNAs function through the negative regulation of their direct targets, the identification of those targets is critical for the understanding of their functional role in breast cancer. METHODS: Breast cancer cell lines were transfected with pre-miR-510 or antisense miR-510 and western blotting and quantitative real time PCR were performed. Functional assays performed included cell growth, migration, invasion, colony formation, cytotoxicity and in vivo tumor growth. We performed a PCR assay to identify novel direct targets of miR-510. The study focused on peroxiredoxin 1 (PRDX1) as it was identified through our screen and was bioinformatically predicted to contain a miR-510 seed site in its 3' untranslated region (3'UTR). Luciferase reporter assays and site-directed mutagenesis were performed to confirm PRDX1 as a direct target. The Student's two-sided, paired t-test was used and a P-value less than 0.05 was considered significant. RESULTS: We show that miR-510 overexpression in non-transformed and breast cancer cells can increase their cell growth, migration, invasion and colony formation in vitro. We also observed increased tumor growth when miR-510 was overexpressed in vivo. We identified PRDX1 through a novel PCR screen and confirmed it as a direct target using luciferase reporter assays. The reintroduction of PRDX1 into breast cancer cell lines without its regulatory 3'UTR confirmed that miR-510 was mediating its migratory phenotype at least in part through the negative regulation of PRDX1. Furthermore, the PI3K/Akt pathway was identified as a positive regulator of miR-510 both in vitro and in vivo. CONCLUSIONS: In this study, we provide evidence to support a role for miR-510 as a novel oncomir. We show that miR-510 directly binds to the 3'UTR of PRDX1 and blocks its protein expression, thereby suppressing migration of human breast cancer cells. Taken together, these data support a pivotal role for miR-510 in breast cancer progression and suggest it as a potential therapeutic target in breast cancer patients.