The presymptomatic treatment with 3HFWC nanosubstance decreased plaque load in 5XFAD mouse model of Alzheimer's disease.

INTRODUCTION: In the present study, we assessed the effects of the hyper-harmonized-hydroxylated fullerene-water complex (3HFWC) on Alzheimer's disease (AD) neuropathological hallmarks in 5XFAD mice, an AD animal model. METHODS: The 3-week-old 5XFAD mice were exposed to 3HFWC water solution ad libitum for 3 months in the presymptomatic phase of pathology. The functional effects of the treatment were confirmed through near-infrared spectroscopy (NIRS) analysis through machine learning (ML) using artificial neural networks (ANNs) to classify the control and 3HFWC-treated brain tissue samples. The effects of 3HFWC treatment on amyloid-ß (Aß) accumulation, plaque formation, gliosis, and synaptic plasticity in cortical and hippocampal tissue were assessed. RESULTS: The 3HFWC treatment significantly decreased the amyloid-ß plaque load in specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not induce the activation of glia (astrocytes and microglia) nor did it negatively affect synaptic protein markers (GAP-43, synaptophysin, and PSD-95). CONCLUSION: The obtained results point to the potential of 3HFWC, when applied in the presymptomatic phase of AD, to interfere with amyloid plaque formation without inducing AD-related pathological processes such as neuroinflammation, gliosis, and synaptic vulnerability.

Predicting anemia using NIR spectrum of spent dialysis fluid in hemodialysis patients.

Anemia is commonly present in hemodialysis (HD) patients and significantly affects their survival and quality of life. NIR spectroscopy and machine learning were used as a method to detect anemia in hemodialysis patients. The aim of this investigation has been to evaluate the near-infrared spectroscopy (NIRS) as a method for non-invasive on-line detection of anemia parameters from HD effluent by assessing the correlation between the spectrum of spent dialysate in the wavelength range of 700-1700 nm and the levels of hemoglobin (Hb), red blood cells (RBC), hematocrit (Hct), iron (Fe), total iron binding capacity (TIBC), ferritin (FER), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) in patient blood. The obtained correlation coefficient (R) for RBC was 0.93, for Hb 0.92, for Fe 0.94, for TIBC 0.96, for FER 0.91, for Hct 0.94, for MCV 0.92, for MCHC 0.92 and for MCH 0.93. The observed high correlations between the NIR spectrum of the dialysate fluid and the levels of the studied variables support the use of NIRS as a promising method for on-line monitoring of anemia and iron saturation parameters in HD patients.

Influence of hyper-harmonized fullerene water complex on collagen quality and skin function.

BACKGROUND: Fullerene water complex establishes the optimal order and function of biomolecules in natural, biophysical way by transducing the signal through water hydrogen bonds to biomolecules. OBJECTIVES: This paper considers the effects of the patented hyper-harmonized-hydroxylated fullerene water complex (3HFWC) on biophysical properties of the skin collagen molecules. METHOD: Optomagnetic imaging spectroscopy (OMIS) has been used for the analysis of the biophysical skin properties (diamagnetic/paramagnetic) after applying three groups of different cosmetic products. Tested cosmetic products were prepared by replacing the active ingredients with 3HFWC or with water in four commercial products. The original commercial creams and their vehiculums with water added served as control groups. Data were statistically analyzed using paired t test in R software. RESULTS: t Test gave statistically significant results for all of the products with 3HFWC, while within the control group, only body lotion and hand cream did show statistically significant results (P < 0.05). Significant improvements in abundance and quality of collagen in the dermis were achieved with body lotion with 3HFWC (p+ /p- ranged from 0.82 to 0.97). While body lotion vehiculum made collagen-water interaction more stable (p+ /p- ranged from 0.3 to 0.55), hand cream with 3HFWC made it more dynamic (p+ /p- ranged from 0.63 to 0.49). Body lotion vehiculum improved the compactness of the dermis (p+ /p- ranged from 0.2 to 1.03), as well as commercial hand cream (p+ /p- ranged from 0.28 to 0.85). CONCLUSION: Compared to the control groups, cosmetic products with 3HFWC demonstrated positive effects on the biophysical properties of the skin. Increased paramagnetic properties are linked to more unpaired electrons, their faster movement, and, finally, better signal transduction. Thus, products with 3HFWC could enable faster regeneration of collagen and prompt skin reaction to the negative environmental influences.

Aquaphotomics approach for monitoring different steps of purification process in water treatment systems.

Currently, the water quality assessment is based on determination of a range of physico-chemical, and microbiological parameters, which is costly, time-consuming and requires multiple tests. Aquaphotomics strategy for water monitoring is based on the principle that the spectra of water are sensitive to all the chemical and physical perturbations, and as such reflect the state of water. In this study, aquaphotomics approach was applied on the near infrared spectra of water samples which underwent different filtration treatments performed by a commercially available water treatment system intended for the household use. The applicability of this approach was demonstrated using difference spectra, multivariate classification analysis and by visualizing the water spectral pattern in aquagrams. The results consistently showed that water samples produced by different filtration treatments have distinctive spectral patterns which characterize the state of water molecular network, and can be used for identification of the respective treatment, tracking efficiency of the treatment and monitoring the performance of water treatment systems.