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1.
Clin Teach ; 21(2): e13671, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-37840475

RESUMEN

INTRODUCTION: Breaking bad news is one of the hardest tasks performed by doctors. The news can significantly impact on the patient's life; however, the process also generates stress for the doctor. The aim of this study was to explore the lived experiences of breaking bad news for obstetrics trainees in Ireland. METHODS: A qualitative study using interpretative phenomenological analysis (IPA) was performed to capture the experience of breaking bad news for the trainees. Semi-structured interviews were conducted with trainees over Zoom. The transcripts were analysed in line with the IPA framework with the assistance of NVivo software. RESULTS: Seven trainees were interviewed, five at Basic Specialist Training (BST) level and two at Higher Specialist Training (HST) level. Four superordinate themes were identified from the analysis: "the reality of working in obstetrics," "the role of the doctor," "development of communication skills" and "the importance of the patient experience." The trainees described breaking bad news in a variety of clinical contexts. This task could be emotionally draining; however, only two trainees mentioned ways of coping with this. Their formal training was limited with the majority of learning occurring "on the job." CONCLUSIONS: This study provides an insight into factors that influence trainees experiences of breaking bad news. The results complemented existing literature and raised questions about how to better support trainees through increased training and psychological support.


Asunto(s)
Médicos , Revelación de la Verdad , Humanos , Médicos/psicología , Escolaridad , Investigación Cualitativa , Comunicación , Relaciones Médico-Paciente
2.
Ir J Psychol Med ; 40(3): 326-329, 2023 09.
Artículo en Inglés | MEDLINE | ID: mdl-36519310

RESUMEN

Reflective practice is increasingly being recognized as an important component of doctors' professional development. Balint group practice is centered on the doctor-patient relationship: what it means, how it may be used to benefit patients, and why it commonly fails owing to a lack of understanding between doctor and patient. The COVID-19 pandemic led to unprecedented disruption to postgraduate medical training programs, including the mandatory Balint groups for psychiatric trainees. This editorial reports on the experience of online Balint groups in the North West of Ireland during the COVID-19 pandemic, and furthermore provides guidance for online Balint group practice into the future.


Asunto(s)
COVID-19 , Educación Médica , Médicos , Humanos , Relaciones Médico-Paciente , Pandemias
3.
Ir J Psychol Med ; 40(3): 369-377, 2023 09.
Artículo en Inglés | MEDLINE | ID: mdl-33455597

RESUMEN

OBJECTIVES: Delirium, which is associated with adverse health outcomes, is poorly detected in hospital settings. This study aimed to determine delirium occurrence among older medical inpatients and to capture associated risk factors. METHODS: This prospective cohort study was performed at an Irish University Hospital. Medical inpatients 70 years and over were included. Baseline assessments within 72 hours of admission included delirium status and severity as determined by the Revised Delirium Rating Scale (DRS-R-98), cognition, physical illness severity and physical functioning. Pre-existing cognitive impairment was determined with Short Informant Questionnaire on Cognitive Decline (IQCODE). Serial assessment of delirium status, cognition and the physical illness severity were undertaken every 3 (±1) days during participants' hospital admission. RESULTS: Of 198 study participants, 92 (46.5%) were women and mean age was 80.6 years (s.d. 6.81; range 70-97). Using DRS-R-98, 17.7% (n = 35) had delirium on admission and 11.6% (n = 23) had new-onset delirium during admission. In regression analysis, older age, impaired cognition and lower functional ability at admission were associated with a significant likelihood of delirium. CONCLUSIONS: In this study, almost one-third of older medical inpatients in an acute hospital had delirium during admission. Findings that increasing age, impaired cognition and lower functional ability at admission were associated with increased delirium risk suggest target groups for enhanced delirium detection and prevention strategies. This may improve clinical outcomes.


Asunto(s)
Delirio , Humanos , Femenino , Adulto , Persona de Mediana Edad , Anciano , Anciano de 80 o más Años , Masculino , Estudios Prospectivos , Delirio/diagnóstico , Delirio/epidemiología , Hospitalización , Pacientes Internos , Hospitales
4.
Ir J Psychol Med ; : 1-7, 2022 May 16.
Artículo en Inglés | MEDLINE | ID: mdl-35575056

RESUMEN

INTRODUCTION AND AIMS: Objective structured clinical examinations (OSCEs) play a pivotal role in medical education assessment. The Advanced Clinical Skills (ACS) OSCE examines clinical skills in psychiatry, general practice, obstetrics and gynaecology and paediatrics. This study examined if the 2020 ACS OSCE for fourth year medical students attending the National University of Ireland, Galway, was associated with any significant result differences compared to the equivalent 2019 OSCE. Additionally, we assessed students' satisfaction and explored any organisational difficulties in conducting a face-to-face OSCE during the COVID-19 pandemic. MATERIALS AND METHODS: This study compared anonymised data between the 2019 and 2020 ACS OSCEs and analysed anonymised student feedback pertaining to the modified 2020 OSCE. RESULTS: The mean total ACS OSCE result achieved in 2020 was statistically higher compared to the 2019 OSCE [62.95% (SD = 6.21) v. 59.35% (SD = 5.54), t = 6.092, p < 0.01], with higher marks noted in psychiatry (p = 0.001), paediatrics (p = 0.001) and general practice (p < 0.001) with more students attaining honours grades (χ2 = 27.257, df = 3, p < 0.001). No difference in failure rates were found. Students reported feeling safe performing the 2020 OSCE (89.2%), but some expressed face-mask wearing impeded their communication skills (47.8%). CONCLUSION: This study demonstrates that conducting a face-to-face OSCE during the pandemic is feasible and associated with positive student feedback. Exam validity has been demonstrated as there was no difference in the overall pass rate.

5.
Ir Med J ; 115(1): 525, 2022 01 20.
Artículo en Inglés | MEDLINE | ID: mdl-35279059

RESUMEN

Presentation A 63-year-old man developed polyarthritis two months post recovery from COVID-19 infection. Diagnosis We concluded that the diagnosis was rheumatoid arthritis based upon raised inflammatory markers, positive rheumatoid factor and anti-cyclic citrullinated peptide antibodies. Treatment His symptoms improved with naproxen, corticosteroids, and methotrexate. Discussion We describe a patient with late onset rheumatoid arthritis possibly triggered or unmasked by COVID-19.


Asunto(s)
Artritis Reumatoide , COVID-19 , Artritis Reumatoide/complicaciones , Artritis Reumatoide/tratamiento farmacológico , Autoanticuerpos , Humanos , Masculino , Persona de Mediana Edad , Péptidos Cíclicos , Factor Reumatoide
7.
Ir J Psychol Med ; 39(4): 409-413, 2022 12.
Artículo en Inglés | MEDLINE | ID: mdl-32912356

RESUMEN

The Coronavirus Disease 2019 (COVID-19) has accounted for more than 25 000 cases in Ireland with approximately 28% of the clusters in nursing homes as of June 2020. The older population is the most vulnerable to serious complications from this illness and over 90% of deaths due to COVID-19 to date have been in patients over the age of 65. Continuing to provide routine care within nursing homes in these challenging times is an essential part of ensuring that presentations to hospitals for non-essential reasons are minimized. In this article, we describe a project being undertaken by a rural Psychiatry of Old Age Service in the northwest of Ireland. We aim to provide ordinary care in extraordinary times by using mobile tablets within the nursing homes and long-stay facilities in our region for remote video consultations during the COVID-19 crisis.


Asunto(s)
COVID-19 , Psiquiatría , Humanos , SARS-CoV-2 , Casas de Salud , Irlanda/epidemiología
8.
Brain Behav Immun ; 89: 423-432, 2020 10.
Artículo en Inglés | MEDLINE | ID: mdl-32726684

RESUMEN

Overactivation of neuroimmune signaling has been linked to excessive ethanol consumption. Toll-like receptors (TLRs) are a major component of innate immune signaling and initiate anti- and pro-inflammatory responses via intracellular signal transduction cascades. TLR7 is upregulated in post-mortem brain tissue from humans with alcohol use disorder (AUD) and animals with prior exposure to ethanol. Despite this evidence, the role of TLR7 in the regulation of voluntary ethanol consumption has not been studied. We test the hypothesis that TLR7 activation regulates voluntary ethanol drinking behavior by administering a TLR7 agonist (R848) during an intermittent access drinking procedure in mice. Acute activation of TLR7 reduced ethanol intake, preference, and total fluid intake due, at least in part, to an acute sickness response. However, chronic pre-treatment with R848 resulted in tolerance to the adverse effects of the drug and a subsequent increase in ethanol consumption. To determine the molecular machinery that mediates these behavioral changes, we evaluated gene expression after acute and chronic TLR7 activation. We found that acute TLR7 activation produces brain region specific changes in expression of immune pathway genes, whereas chronic TLR7 activation causes downregulation of TLRs and blunted cytokine induction, suggesting molecular tolerance. Our results demonstrate a novel role for TLR7 signaling in regulating voluntary ethanol consumption. Taken together, our findings suggest TLR7 may be a viable target for development of therapies to treat AUD.


Asunto(s)
Alcoholismo , Receptor Toll-Like 7 , Consumo de Bebidas Alcohólicas , Animales , Etanol , Ratones , Ratones Endogámicos C57BL , Receptores Toll-Like
11.
Osteoarthritis Cartilage ; 28(5): 603-612, 2020 05.
Artículo en Inglés | MEDLINE | ID: mdl-31730805

RESUMEN

OBJECTIVE: A number of studies have demonstrated that molecules called 'alarmins' or danger-associated molecular patterns (DAMPs), contribute to inflammatory processes in the OA joint. Metabolic reprogramming of immune cells, including macrophages, is emerging as a prominent player in determining immune cell phenotype and function. The aim of this study was to investigate if basic calcium phosphate (BCP) crystals which are OA-associated DAMPs, impact on macrophage phenotype and metabolism. METHODS: Human monocyte derived macrophages were treated with BCP crystals and expression of M1 (CXCL9, CXCL10) and M2 (MRC1, CCL13)-associated markers was assessed by real-time PCR while surface maturation marker (CD40, CD80 & CD86) expression was assessed by flow cytometry. BCP induced metabolic changes were assessed by Seahorse analysis and glycolytic marker expression (hexokinase 2(HK2), Glut1 and HIF1α) was examined using real-time PCR and immunoblotting. RESULTS: Treatment with BCP crystals upregulated mRNA levels of CXCL9 and CXCL10 while concomitantly downregulating expression of CCL13 and MRC1. Furthermore, BCP-treated macrophages enhanced surface expression of the maturation makers, CD40, CD80 and CD86. BCP-treated cells also exhibited a shift towards glycolysis as evidenced by an increased ECAR/OCR ratio and enhanced expression of the glycolytic markers, HK2, Glut1 and HIF1α. Finally, BCP-induced macrophage activation and alarmin expression was reduced in the presence of the glycolytic inhibitor, 2-DG. CONCLUSIONS: This study not only provides further insight into how OA-associated DAMPs impact on immune cell function, but also highlights metabolic reprogramming as a potential therapeutic target for calcium crystal-related arthropathies.


Asunto(s)
Fosfatos de Calcio/farmacología , Citocinas/efectos de los fármacos , Glucólisis/efectos de los fármacos , Macrófagos/efectos de los fármacos , Osteoartritis/inmunología , Antígeno B7-1/metabolismo , Antígeno B7-2/metabolismo , Antígenos CD40/metabolismo , Quimiocina CXCL10/efectos de los fármacos , Quimiocina CXCL10/genética , Quimiocina CXCL10/inmunología , Quimiocina CXCL9/efectos de los fármacos , Quimiocina CXCL9/genética , Quimiocina CXCL9/inmunología , Citocinas/genética , Regulación hacia Abajo , Transportador de Glucosa de Tipo 1/efectos de los fármacos , Transportador de Glucosa de Tipo 1/genética , Transportador de Glucosa de Tipo 1/metabolismo , Glucólisis/genética , Hexoquinasa/efectos de los fármacos , Hexoquinasa/genética , Hexoquinasa/metabolismo , Humanos , Subunidad alfa del Factor 1 Inducible por Hipoxia/efectos de los fármacos , Subunidad alfa del Factor 1 Inducible por Hipoxia/genética , Subunidad alfa del Factor 1 Inducible por Hipoxia/metabolismo , Activación de Macrófagos , Macrófagos/inmunología , Macrófagos/metabolismo , Glicoproteínas de Membrana/efectos de los fármacos , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/inmunología , Proteínas Quimioatrayentes de Monocitos/efectos de los fármacos , Proteínas Quimioatrayentes de Monocitos/genética , Proteínas Quimioatrayentes de Monocitos/inmunología , Osteoartritis/genética , Fenotipo , ARN Mensajero/efectos de los fármacos , ARN Mensajero/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Receptores Inmunológicos/efectos de los fármacos , Receptores Inmunológicos/genética , Receptores Inmunológicos/inmunología , Regulación hacia Arriba
12.
Am J Mens Health ; 13(1): 1557988319832121, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31068064

RESUMEN

This research sought to establish the impact of a 10-week program combining mixed martial arts (MMA) and one-to-one psychotherapy on young males' mental health and determine factors that predict help-seeking behavior in at-risk males. Preparticipation and post-participation interviews were employed as the method of data collection. Seven males (20-35 years; M = 24.57) completed preparticipation interviews and five completed follow-up interviews. Thematic analysis of preparticipation revealed that help-seeking behavior in at-risk males is impeded by the presence of male gender stereotypes, the absence of positive role models, as well as difficulty navigating challenging social landscapes. Post-participation interviews revealed that the sport provided structure and fitness for at-risk males, while the counseling was pivotal for personal growth. Improved relationships, work life, and self-esteem were also observed. The sporting element of the program helped to reduce stigma associated with engaging in psychotherapy, and positive male relationships were noted as particularly impactful. Findings support previous research indicating that combining sports and psychotherapy positively impacts young males' mental health. Sport provides an acceptable doorway to psychotherapy, providing space to explore personal issues.


Asunto(s)
Artes Marciales , Salud del Hombre , Salud Mental , Psicoterapia/métodos , Adulto , Conducta de Búsqueda de Ayuda , Humanos , Masculino , Estigma Social , Estereotipo
13.
Parasitology ; 146(6): 746-752, 2019 May.
Artículo en Inglés | MEDLINE | ID: mdl-30561289

RESUMEN

To date, there are no recent studies identifying the prevalence of parasites of human and veterinary importance in dogs and cats in Ireland. The interaction between pets and wildlife species in the environment is an important source of parasite exposure to canids and felines, and one likely to be heightened in the stray animal population. This study aimed to establish the prevalence of endoparasites in unowned dogs and cats in County Dublin, Ireland. Feces from stray dogs (n = 627) and cats (n = 289) entering a rehoming centre were collected immediately after defecation. The main parasitic agents detected were ascarids (15.52 and 30.26%), Cystoisospora (3.27 and 3.69%), Giardia spp. (6.02 and 1.84%) and lungworms (0.64 and 2.08%), in dogs and cats respectively. Animals younger than 3 months of age were more likely to be infected with ascarids (P < 0.001) and Cystoisospora spp. (P = 0.008 and P = 0.014) than older animals. All lungworms were morphologically identified and dogs were infected with Angiostrongylus vasorum (0.48%) and Crenosoma vulpis (0.16%) whereas cats were only infected with Aelurostrongylus abstrusus (2.08%). This represents the first prevalence study of stray animals in Ireland. Data collected will inform the treatment and in addition, the future monitoring and control studies of parasite populations.

14.
Ir J Psychol Med ; 35(3): 193-201, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-30124183

RESUMEN

OBJECTIVES: This study estimates the symptomatology of attention deficit-hyperactivity disorder (ADHD) in adult mental health services (AMHS) outpatient clinics. METHODS: All consecutive patients attending any of the outpatients' clinics in Sligo/Leitrim AMHS were invited to participate. Participants completed the Adult ADHD Self-Report Scale (ASRS) and the Wender Utah Rating Scale (WURS) self-report. Clinical notes were reviewed to identify those with a pre-existing ADHD diagnosis. RESULTS: From 822 attending the clinics, 62 did not meet inclusion criteria, 97 declined to participate and 29 had incomplete data in either of the screening scales, leaving 634 (77%) eligible for full study analysis. Mean age was 40.38 (s.d.: 12.85), and 326 (51.4%) were females. In total, 215 (33.9%) screened positive on the WURS for childhood onset ADHD and 219 (34.5%) participants scored positive on the ASRS. Applying a more stringent criteria of scoring above cut-offs on both scales, suggested 131 (20.7%) screened positive on both. Only three (2.3%) had a prior clinical diagnosis. CONCLUSIONS: This preliminary study suggests the possibility of relatively higher rates of ADHD in a general AMHS than previously thought, however, given the possibility of overlapping symptoms with other major psychiatric disorders in adulthood and recall bias further research is needed before drawing firm conclusions.


Asunto(s)
Trastorno por Déficit de Atención con Hiperactividad/epidemiología , Adulto , Instituciones de Atención Ambulatoria/estadística & datos numéricos , Trastorno por Déficit de Atención con Hiperactividad/diagnóstico , Femenino , Humanos , Masculino , Persona de Mediana Edad , Escalas de Valoración Psiquiátrica , Autoinforme , Índice de Severidad de la Enfermedad
15.
Ir J Psychol Med ; 35(1): 43-51, 2018 03.
Artículo en Inglés | MEDLINE | ID: mdl-30115202

RESUMEN

OBJECTIVES: Providing care for a person with dementia or other chronic illness at home often places stress on the primary caregiver. In an Irish population, ~67% of carers reported experiencing extreme physical or mental tiredness. This study aimed to identify factors that influence carer burden and identify the sub-populations of carers who are most susceptible to burden. METHODS: Consecutive carers referred to a local carers' support organisation completed the following measurements: the Neuropsychiatric Inventory, Zarit Burden Interview, Social Network Index, General Health Questionnaire, Short Form Survey, Hamilton Depression Rating Scale, Brown's Locus of Control scale and provided demographic data on themselves and their patient. RESULTS: The sample consisted 53 carers, mean age: 64.5±11.7, of whom 43 (81.1%) were females. A linear regression model found significant independent (p<0.05) factors for carer burden were: increased behavioural problems of the patient, carer characteristics including female gender, younger age, high number of contacts, lower physical functioning and emotional problems, while protective factors were marriage and higher number of embedded networks. CONCLUSIONS: The ability to predict which carers are more susceptible to burden allows service providers to more quickly and accurately identify 'higher risk' carers, facilitating routine check-ups by physicians and carer support services.


Asunto(s)
Adaptación Psicológica , Cuidadores/psicología , Resiliencia Psicológica , Apoyo Social , Factores de Edad , Estudios Transversales , Femenino , Humanos , Irlanda , Masculino , Persona de Mediana Edad , Escalas de Valoración Psiquiátrica , Calidad de Vida , Factores de Riesgo , Factores Sexuales
16.
Ann Rheum Dis ; 76(2): 318-328, 2017 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-27377815

RESUMEN

OBJECTIVE: The original European League Against Rheumatism recommendations for managing fibromyalgia assessed evidence up to 2005. The paucity of studies meant that most recommendations were 'expert opinion'. METHODS: A multidisciplinary group from 12 countries assessed evidence with a focus on systematic reviews and meta-analyses concerned with pharmacological/non-pharmacological management for fibromyalgia. A review, in May 2015, identified eligible publications and key outcomes assessed were pain, fatigue, sleep and daily functioning. The Grading of Recommendations Assessment, Development and Evaluation system was used for making recommendations. RESULTS: 2979 titles were identified: from these 275 full papers were selected for review and 107 reviews (and/or meta-analyses) evaluated as eligible. Based on meta-analyses, the only 'strong for' therapy-based recommendation in the guidelines was exercise. Based on expert opinion, a graduated approach, the following four main stages are suggested underpinned by shared decision-making with patients. Initial management should involve patient education and focus on non-pharmacological therapies. In case of non-response, further therapies (all of which were evaluated as 'weak for' based on meta-analyses) should be tailored to the specific needs of the individual and may involve psychological therapies (for mood disorders and unhelpful coping strategies), pharmacotherapy (for severe pain or sleep disturbance) and/or a multimodal rehabilitation programme (for severe disability). CONCLUSIONS: These recommendations are underpinned by high-quality reviews and meta-analyses. The size of effect for most treatments is relatively modest. We propose research priorities clarifying who will benefit from specific interventions, their effect in combination and organisation of healthcare systems to optimise outcome.


Asunto(s)
Actividades Cotidianas , Fatiga/terapia , Fibromialgia/terapia , Guías de Práctica Clínica como Asunto , Sueño , Terapia por Acupuntura , Amitriptilina/análogos & derivados , Amitriptilina/uso terapéutico , Antiinflamatorios no Esteroideos/uso terapéutico , Anticonvulsivantes/uso terapéutico , Antidepresivos Tricíclicos/uso terapéutico , Biorretroalimentación Psicológica , Capsaicina/uso terapéutico , Terapia Cognitivo-Conductual , Europa (Continente) , Medicina Basada en la Evidencia , Terapia por Ejercicio , Fatiga/fisiopatología , Fibromialgia/fisiopatología , Hormona de Crecimiento Humana/uso terapéutico , Humanos , Hidroterapia , Hipnosis , Manipulación Quiropráctica , Masaje , Terapias Mente-Cuerpo , Atención Plena , Inhibidores de la Monoaminooxidasa/uso terapéutico , Dolor/fisiopatología , S-Adenosilmetionina/uso terapéutico , Fármacos del Sistema Sensorial/uso terapéutico , Inhibidores Selectivos de la Recaptación de Serotonina/uso terapéutico , Inhibidores de Captación de Serotonina y Norepinefrina/uso terapéutico , Sociedades Médicas , Oxibato de Sodio/uso terapéutico , Resultado del Tratamiento
17.
Osteoarthritis Cartilage ; 24(12): 2141-2152, 2016 12.
Artículo en Inglés | MEDLINE | ID: mdl-27426968

RESUMEN

OBJECTIVE: Basic calcium phosphate (BCP) and monosodium urate (MSU) crystals are particulates with potent pro-inflammatory effects, associated with osteoarthritis (OA) and gout, respectively. Bone erosion, due to increased osteoclastogenesis, is a hallmark of both arthropathies and results in severe joint destruction. The aim of this study was to investigate the effect of these endogenous particulates on anti-osteoclastogenic cytokine signalling. METHODS: Human osteoclast precursors (OcP) were treated with BCP and MSU crystals prior to stimulation with Interleukin (IL-6) or Interferon (IFN-γ) and the effect on Signal Transducer and Activator of Transcription (STAT)-3 and STAT-1 activation in addition to Mitogen Activated Protein Kinase (MAPK) activation was examined by immunoblotting. Crystal-induced suppressor of cytokine signalling (SOCS) protein and SH-2 containing tyrosine phosphatase (SHP) expression was assessed by real-time polymerase chain reaction (PCR) in the presence and absence of MAPK inhibitors. RESULTS: Pre-treatment with BCP or MSU crystals for 1 h inhibited IL-6-induced STAT-3 activation in human OcP, while pre-treatment for 3 h inhibited IFN-γ-induced STAT-1 activation. Both crystals activated p38 and extracellular signal-regulated (ERK) MAPKs with BCP crystals also activating c-Jun N-terminal kinase (JNK). Inhibition of p38 counteracted the inhibitory effect of BCP and MSU crystals and restored STAT-3 phosphorylation. In contrast, STAT-1 phosphorylation was not restored by MAPK inhibition. Finally, both crystals potently induced the expression of SOCS-3 in a MAPK dependent manner, while BCP crystals also induced expression of SHP-1 and SHP-2. CONCLUSION: This study provides further insight into the pathogenic effects of endogenous particulates in joint arthropathies and demonstrates how they may contribute to bone erosion via the inhibition of anti-osteoclastogenic cytokine signalling. Potential targets to overcome these effects include p38 MAPK, SOCS-3 and SHP phosphatases.


Asunto(s)
Transducción de Señal , Fosfatos de Calcio , Humanos , Interleucina-6 , Proteínas Quinasas JNK Activadas por Mitógenos , Proteínas Quinasas Activadas por Mitógenos , Ácido Úrico
18.
Vet Parasitol ; 220: 54-8, 2016 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-26995721

RESUMEN

Angiostrongylosis is potentially fatal parasitic nematode infection affecting dogs which can be difficult to diagnose. In recent years several microscopical, serological and molecular detection methods have been developed, however there are few studies that have compared the relative performance of these methods. Screening necropsy material from an opportunistic sample of 140 foxes (82 of which were considered to be infected with Angiostrongylus vasorum), indicated sensitivities of 84.1% for dissection and visual examination of plucks, 69.5% for nested PCR of an 18S rRNA fragment and 76.8% for a canine A. vasorum antigen detection test (IDEXX Angio Detect) of tissue fluid samples respectively. Agreement between the tests ranged from 45.6 to 79.7%. A novel nested PCR-RFLP for the detection and identification of canid lungworm spp. is described.


Asunto(s)
Disección/normas , Ensayo de Inmunoadsorción Enzimática/normas , Zorros/parasitología , Técnicas de Diagnóstico Molecular/normas , Parasitología/métodos , Infecciones por Strongylida/diagnóstico , Angiostrongylus/genética , Angiostrongylus/aislamiento & purificación , Animales , Antígenos Helmínticos/análisis , ARN Ribosómico 18S/genética , Reproducibilidad de los Resultados , Sensibilidad y Especificidad
19.
Parasitology ; 143(5): 588-93, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-26940534

RESUMEN

The reported incidence of the metastrongylid nematode Angiostrongylus vasorum, that infects dogs and other canids, is increasing worldwide outside recognized endemic foci. This apparent expansion of the parasite's range is causing concern to veterinary clinicians as the disease caused in dogs can be life threatening and its treatment is not straightforward. The red fox is thought to be a reservoir host for dogs. To investigate the spatial distribution of infection in foxes in Ireland, the hearts and lungs of 542 foxes from all over Ireland were examined. The incidence of infection was found to be 39·9% [95% confidence interval (CI) 35·7-44·1] with positive samples occurring in each of the country's 26 counties. This report confirms that the parasite is endemic in Ireland and the overall prevalence is the second highest in Europe. This is the first survey of A. vasorum infection in Irish foxes and highlights the potential exposure of the Irish dog population to high risk of cross-infection. Additionally, Crenosoma vulpis was found in seven of the foxes, a parasite not previously reported in the Irish fox.


Asunto(s)
Angiostrongylus/aislamiento & purificación , Zorros/parasitología , Infecciones por Strongylida/veterinaria , Animales , Enfermedades Endémicas/veterinaria , Corazón/parasitología , Irlanda/epidemiología , Pulmón/parasitología , Prevalencia , Infecciones por Strongylida/epidemiología , Infecciones por Strongylida/parasitología , Tráquea/parasitología
20.
Sci Total Environ ; 514: 140-6, 2015 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-25659312

RESUMEN

The objective was to investigate the removal of Salmonella and enteric indicator bacteria from the liquid fraction of raw and anaerobically digested (AD) pig manure in woodchip biofilters over a 14 week (98 day) period. Antibiotic susceptible Salmonella Infantis was detected in one influent material (liquid fraction of raw manure) on two occasions but was not found in the effluent at any time point. Furthermore, mean coliform reductions of 56% were observed in the biofilters treating the liquid fraction of raw manure. However, a mean increase of 228% was found in those treating the liquid from AD manure, despite the fact that the microbial challenge to these biofilters was lower. In addition, relatively high coliform counts were still present in the effluent from both biofilter treatments, especially in the systems treating the liquid fraction of AD manure. However, findings for Escherichia coli and Enterococcus were more promising, with reductions observed for both treatments (10 and 18.5% for E. coli and 71 and 87% for Enterococcus). Moreover, E. coli and Enterococcus were at, or just above, the limit of detection in the final effluents. Overall, although, there are no microbial limits for discharge or washwaters, the woodchip filter effluent would appear safe for discharge to waterways or use on-farm as regards Salmonella, E. coli and Enterococcus but not coliform. In conclusion, woodchip biofilters offer potential as a low-cost sustainable novel treatment option for the removal of pathogens from the liquid fraction of pig manure.


Asunto(s)
Filtración/métodos , Estiércol/microbiología , Salmonella/aislamiento & purificación , Eliminación de Residuos Líquidos/métodos , Anaerobiosis , Animales , Enterobacteriaceae , Porcinos
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