[Inhibition of the stellate cells using lisinopril and lovastatin for prophylaxis of pancreatic stump fibrosis after performance of distal resection in a model of chronic alcoholic pancreatitis].

Complex application of lisinopril (inhibitor of angiotensin-converting enzyme) and lovastatin (inhibitor of HMG-CoA reductase) inhibits pancreatic fibrosis in the rats, having alcoholic chronic pancreatitis after distal pancreatic resection (DPR). Lisinopril and lovastatin were injected to the rats after DPR in dose 10 mg/kg during 3 weeks. Immunohistochemical markets, such as alpha-smooth-muscle actin (alpha-SMA), desmin, glial fibrillary acidic protein (GFAP), vimentin, and expression of matrix metalloproteinase-1 (MMP-1) as well as inhibitor of matrix metalloproteinase-2 (TIMP-2) were detected for estimation of therapeutic impact on activity and quantity of stellate pancreatic cells. Under the influence of lisinopril and lovastatin there were observed lowering in the stellate pancreatic cells activity and in expression of SMA, desmin, GFAP, vimentin and TIMP-2, the MMP-1 and TIMP-2 ratio have had increased significantly and severity of fibrotic pancreatic affection had reduced trustworthy in comparison w such, occurring in a control group.

[Inhibition of pancreatic stellate cell activation by lisinopril for prevention fibrogenesis in experimental chronic alcoholic pancreatitis].

Lisinopril (angiotensin-converting enzyme inhibitor) attenuates fibrotic changes in pancreas after distal pancreatectomy in rats with experimental alcohol induced chronic pancreatitis. Lisinopril was administered after distal pancreatectomy in rats with experimental alcohol induced chronic pancreatitis. The animals were treated with lisinopril at the dose of 10 mg/kg body weight per day for 21 days after operation. To estimate the efficacy of the treatment on activity and number of pancreatic stellate cells the immunohistochemical investigation was made with alpha-smooth muscle actin (alpha-SMA), desmin, vimentin, glial fibrillary acidic protein (GFAP), matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase-2 (TIMP-2) using. The treatment of rats after operation with lisinopril inhibite activity of pancreatic stellate cells and characterized by significant decrease of the alpha-SMA, desmin, GFAP, vimentin and TIMP-2 expression. The ratio of MMP-1/TIMP-2 was greater in the group with treatment then in the control group. This therapy had a trend to alleviate the fibrotic changes in pancreas.

[The "tourniquet" ischemia-reoxygenation of the extremities as the method of nonmedicinal immunocorrection].

There were adduced the results of studying of cellular and humoral factors of the immunity in a brief-lasting ischemia-reoxygenation of the extremities in 56 patients with surgical diseases and in 31 pupils, in whom the organism reactivity lowering was revealed. It was established, that changes in the ischemia-reoxygenation of the extremities are multifactorial and they are connected primarily with the immunocompetent cells activation. Nonmedicinal method of the "tourniquet" immunocorrection was proposed, which is recommended as a concurrent one - for the recovery acceleration or solely - in the treatment of inflammatory diseases. It may be prescribed as a health-improving method in a rapid fatigue, insomnia, weakness, loss of appetite and other signs of the organism reactivity lowering. Application of the method secures the morbidity lowering in frequently ill pupils.

[Main principles of antibiotic prophylaxis and antibacterial therapy in abdominal surgery].

The principles of antibioticoprophylaxis and antibacterial therapy in abdominal surgery were expounded. There are adduced the most frequently revealed pathogenic microorganisms, inducing intraabdominal infection, the ways of lowering of the antibacterial therapy expenses, new approaches to application of immunocorrection and directed transport of antibiotics in surgery as well as empirical and etiotropic treatment, basing on the taking account of the spread and antibioticosensitivity of pathogenic microorganisms of nosocomial infections. There was shown, that concentration of interleucin-1beta and tumor necrosis factor-alpha in the blood serum is connected with the severity of patient state. Significant rising of this cytokines concentration constitutes the trustworthy criterion of the ineffective therapy and mortality risk. The influence of microorganisms and some antibiotics on induction of interferons was studied. Amoxicyllin, polymixin, vancomycin, cephalosporins and fluconazol are the most active inductors of gamma-interferon. Maximal efficacy of antibacterial therapy is possible only if an adequate surgical intervention was performed.

[Effect of spore-free anaerobic microorganisms of pancreatic tissue in experimental acute pancreatitis]. / Vozdeistvie nesporoobrazuiushchikh anaérobnykh mikroorganizmov na tkan' podzheludochnoi zhelezy pri éksperimental'nom ostrom pankreatite.

The simulation model of an acute pancreatitis (AP), using translocation of microorganisms from intestine to ductal system and pancreatic tissue, was created in experiment on dogs. Interrelationships between pancreatic tissue and anaerobic microorganism in an AP were studied, using electron microscopy. Possibility of the microorganisms migration to pancreas from ductal system in early stage of AP due to enhanced reproduction of anaerobic microorganisms in duodenum was established. It is impossible to exclude the possibility of their invasion via the lymph and the blood flow into destructively changed pancreatic tissue in late terms of the disease.

[Chronic venous insufficiency of the lower extremities in patients with post-thrombophlebitic syndrome]. / Kronicheskaia venoznaia nedostatochnost' nizhnikh konechnostei u bol'nykh s posttromboflebiticheskim sindromom.

The results of examination and treatment of 734 patients with postthrombophlebitic syndrome of lower extremities were adduced. The pathogenesis diagram for chronic venous insufficiency was proposed. The possibilities of the regional blood flow correction using reconstructive operations on veins were shown. In 73% of patients good result in immediate postoperative period was noted and in late follow-up period--in 64%.

[The cytological aspects of chronic pancreatitis]. / Tsitologicheskie aspekty khronicheskogo pankreatita.

Biopsy material of pancreatic tissue using light-optical radioautography and electron microscopy, and the method of high-precise radioautography also, was investigated to study up the pancreatic gland (PG) pathology in chronic pancreatitis (CHP). It was established that CHP is a chronic proliferative-destructive process causing focal generalized fibrosis of PG. All the structural components of organ, and the duct epithelium first of all, are affected in CHP. It gives reason to regard a duct system as a complex independent morphofunctional unit of PG.

[Antiviral action of the macromolecular derivative of the platinum with deoxyribonucleic acid in the treatment of patient with malignant liver neoplasm]. / Protivovirusnoe deistvie makromolekuliarnogo proizvodnogo platiny s dezoksiribonukleinovoi kislotoi u bolnykh so zlokachestvennoi opukholíu pecheni.

Results of the action of a preparation consisting of cis-dichlorodiammineplatinum and deoxyribonucleic acid combined in the saline balanced solution by means of the original technique on cytomegalovirus infection of patients with cancer of the liver are presented. Clearly pronounced antivirus activity of the preparation developed is noted.

[The effect of polysorb on the course of the wound process]. / Vliianie polisorba na techenie ranevogo protsessa.

The peculiarities of a contact of the sorbent particles of a polysorb for medical purposes with the somatic cells have been studied. The destructive action of the preparation its capability to block the functionally active sites of a cellular membrane were revealed. The recommendations on clinical use of a polysorb have been developed.

[Assessment of the membrane properties of blood leukocytes in patients with purulent septic complications]. / Otsenka membrannykh svoistv leikotsitov krovi u bolnykh s gnoino-septicheskimi oslozhneniiami.

Osmotic resistance of leukocytic membranes was studied in 36 patients with purulent-septic complications and in 43 donors. As a damaging medium, 0.45% solution of sodium chloride was used. The cells were incubated in it for 30 min at a temperature of 37 degrees C. It was established that in purulent-septic complications, the structural-functional properties of leukocytes changed. This resulted in decreased resistance of their membranes.

[The cytological diagnosis of liver cancer]. / O tsitologicheskoi diagnostike raka pecheni.

A study is presented of 96 patients with primary cancer of the liver. Hepatocellular cancer was diagnosed in 54 patients, cholangiocellular--in 36, low-differentiated--in 6 patients. Hepatocellular cancer was characterized by an increased size of the cells while they maintained likeness with intact liver cells, were characterized by significant anisocytosis, hyperchromia, reticular cytoplasm. Cholangiocellular forms of cancer are characterized by the presence of large nucleoli, cytoplasm vacuolization. Nondifferentiated cancer was distinguished by markedly pronounced polymorphism, presence of "nude" nuclei, disorders of the nucleus/cytoplasm ratio in favour of the nucleus, presence of syncytial relations between the cells.

[Ultrastructural changes in neutrophils and anaerobic microorganisms during their interactions in experimental acute intestinal obstruction]. / Ul'trastrukturnye izmeneniia neitrofil'nykh granulotsitov i anaérobnykh mikroorganizmov pri ikh vzaimodeistvii v usloviiakh éksperimental'noi ostroi neprokhodimosti kishechnika.

The authors established that 12 h after development of acute obstructive ileus in inactive neutrophil granulocytes (NG), the anaerobic microorganisms were intact, or at the process of division; in the active NG, they were destroyed, or when forming "halo", might preserve and migrate in the matrix of NG. In interaction of NG with microorganisms, the numerous oval and roundish bacterial bodies with a diameter of 0.2-0.5 micron capable to penetrate into the non-phagocytic cells are formed. A function of the formations revealed is not studied.