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The epitranscriptomic mark N 6-methyladenosine (m6A) is the most common type of messenger RNA (mRNA) post-transcriptional modification in eukaryotes. With the discovery of the demethylase FTO (FAT MASS AND OBESITY-ASSOCIATED PROTEIN) in Homo Sapiens, this modification has been proven to be dynamically reversible. With technological advances, research on m6A modification in plants also rapidly developed. m6A modification is widely distributed in plants, which is usually enriched near the stop codons and 3'-UTRs, and has conserved modification sequences. The related proteins of m6A modification mainly consist of three components: methyltransferases (writers), demethylases (erasers), and reading proteins (readers). m6A modification mainly regulates the growth and development of plants by modulating the RNA metabolic processes and playing an important role in their responses to environmental signals. In this review, we briefly outline the development of m6A modification detection techniques; comparatively analyze the distribution characteristics of m6A in plants; summarize the methyltransferases, demethylases, and binding proteins related to m6A; elaborate on how m6A modification functions in plant growth, development, and response to environmental signals; and provide a summary and outlook on the research of m6A in plants.
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Background: The antibiotic resistance in various bacteria is consistently increasing and is posing a serious threat to human health, prompting the need for the discovery of novel structurally featured natural products with promising biological activities in drug research and development. Endolichenic microbes have been proven to be a fertile source to produce various chemical components, and therefore these microbes have been on a prime focus for exploring natural products. In this study, to explore potential biological resources and antibacterial natural products, the secondary metabolites of an endolichenic fungus have been investigated. Methods: The antimicrobial products were isolated from the endolichenic fungus using various chromatographic methods, and the antibacterial and antifungal activities of the compounds were evaluated by the broth microdilution method under in vitro conditions. The antimicrobial mechanism has been discussed with measuring the dissolution of nucleic acid and protein, as well as the activity of alkaline phosphatase (AKP) in preliminary manner. Chemical synthesis of the active product compound 5 was also performed, starting from commercially available 2,6-dihydroxybenzaldehyde through a sequence of transformations that included methylation, the addition of propylmagnesium bromide on formyl group, the oxidation of secondary alcohol, and the deprotection of methyl ether motif. Results: Among the 19 secondary metabolites of the endolichenic fungus, Daldinia childiae (compound 5) showed attractive antimicrobial activities on 10 of the 15 tested pathogenic strains, including Gram-positive bacteria, Gram-negative bacteria, and fungus. The Minimum Inhibitory Concentration (MIC) of compound 5 for Candida albicans 10213, Micrococcus luteus 261, Proteus vulgaris Z12, Shigella sonnet, and Staphylococcus aureus 6538 was identified as 16 µg/ml, whereas the Minimum Bactericidal Concentration (MBC) of other strains was identified as 64 µg/ml. Compound 5 could dramatically inhibit the growth of S. aureus 6538, P. vulgaris Z12, and C. albicans 10213 at the MBC, likely affecting the permeability of the cell wall and cell membrane. These results enriched the library of active strains and metabolites resources of endolichenic microorganisms. The chemical synthesis of the active compound was also performed in four steps, providing an alternative pathway to explore antimicrobial agents.
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Although nitrogen (N) fertilizer application plays an essential role in improving crop productivity, an inappropriate management can result in negative impacts on environment and human health. To break this dilemma, a 12-year field experiment (2008-2019) with five N application rates was conducted on the North China Plain (NCP) to evaluate the integrated impacts of optimizing N management (Opt. N, 160 kg N ha-1 on average) on agronomic, environmental, health, and economic performances of summer maize production. Over the 12-year study, the Opt. N treatment achieved the maximal average grain yield (10.6 Mg ha-1) and grain protein yield (793 kg ha-1) among five N treatments. The life cycle assessment methodology was applied to determine the negative impacts on environmental and human health, and both of them increased with the N rate. Compared with the farmers' conventional N rate (250 kg N ha-1), the Opt. N treatment reduced acidification, eutrophication, global warming, and energy depletion potentials by 29%, 42%, 35%, and 18%, respectively, and reduced the health impact by 32% per Mg of grain yield or grain protein yield produced. Both the Opt. N and Opt. N*50-70% treatments resulted in high private profitability (2038 USD ha-1), ecosystem economic benefit (1811 USD ha-1), and integrated compensation benefit (17,548 USD ha-1). This study demonstrates the potential benefits of long-term optimizing of N management to maintain high maize yields and grain quality, to reduce various environmental impacts and health impacts, and to enhance economic benefits. These benefits can be further enhanced when Opt. N was combined with advanced agronomic management practices. The results also suggest that reducing the optimal N rate from 160 to 145 kg N ha-1 is achievable to further reduce the negative impacts while maintaining high crop productivity. In conclusion, optimizing the N management is essential to promote sustainable summer maize production on the NCP.
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Toxoplasma gondii is a worldwide zoonotic protozoan. Donkeys are often susceptible to many pathological agents, acting as carriers of pathogens for other animal species and humans. However, data on the prevalence of T. gondii in donkeys during lactation and on the status of antibodies against T. gondii in donkey milk are lacking. A cross-sectional study evaluated the variation of the anti-T. gondii antibodies in the blood and milk of domestic donkeys during lactation. A total of 418 domestic donkeys were randomly selected from the Shandong province, eastern China from January 2019 to March 2020. The anti-T. gondii antibodies were found in 11.72% (49/418) serum and 9.81% (41/418) milk samples using a commercial ELISA kit, respectively. There was a very high consistency between the serum and milk (Spearman's coefficient = 0.858, p-value < 0.0001 and Kendall's tau = 0.688, p-value < 0.0001), particularly at the 45th to 60th day of lactation. The present results of the statistical analysis showed that the history of abortion (p = 0.026; adjusted OR = 2.20; 95% CI: 1.15-4.20) and cat in the house (p = 0.008; adjusted OR = 2.36; 95% CI: 1.26-4.44) were significantly associated with T. gondii infection in the domestic donkeys. This is the first report to detect antibodies against T. gondii in donkey milk in China. These results indicate a potential risk of humans contracting the infection through the consumption of raw milk from the naturally infected donkeys.
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Toxoplasma , Toxoplasmosis Animal , Animales , Anticuerpos Antiprotozoarios , China/epidemiología , Estudios Transversales , Equidae , Femenino , Inmunoglobulina G , Lactancia , Leche , Embarazo , Salud Pública , Toxoplasmosis Animal/epidemiologíaRESUMEN
Three new species of Russulasection Ingratae, found in Guizhou and Jiangsu Provinces, southern China, are proposed: R.straminella, R.subpectinatoides and R.succinea. Photographs, line drawings and detailed morphological descriptions for these species are provided with comparisons against closely-related taxa. Phylogenetic analysis of the internal transcribed spacer (ITS) region supported the recognition of these specimens as new species. Additionally, R.indocatillus is reported for the first time from China and morphological and phylogenetic data are provided for the Chinese specimens.
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One of the best ways to control COVID-19 is vaccination. Among the various SARS-CoV-2 vaccines, inactivated virus vaccines have been widely applied in China and many other countries. To understand the underlying protective mechanism of these vaccines, it is necessary to systematically analyze the humoral responses that are triggered. By utilizing a SARS-CoV-2 microarray with 21 proteins and 197 peptides that fully cover the spike protein, antibody response profiles of 59 serum samples collected from 32 volunteers immunized with the inactivated virus vaccine BBIBP-CorV were generated. For this set of samples, the microarray results correlated with the neutralization titers of the authentic virus, and two peptides (S1-5 and S2-22) were identified as potential biomarkers for assessing the effectiveness of vaccination. Moreover, by comparing immunized volunteers to convalescent and hospitalized COVID-19 patients, the N protein, NSP7, and S2-78 were identified as potential biomarkers for differentiating COVID-19 patients from individuals vaccinated with the inactivated SARS-CoV-2 vaccine. The comprehensive profile of humoral responses against the inactivated SARS-CoV-2 vaccine will facilitate a deeper understanding of the vaccine and provide potential biomarkers for inactivated virus vaccine-related applications.
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Anticuerpos Antivirales/inmunología , COVID-19/inmunología , Epítopos/química , Inmunoglobulina G/inmunología , SARS-CoV-2/inmunología , Glicoproteína de la Espiga del Coronavirus/química , Glicoproteína de la Espiga del Coronavirus/inmunología , Aminoácidos/química , Epítopos/genética , Humanos , Análisis por Matrices de Proteínas , Glicoproteína de la Espiga del Coronavirus/genéticaRESUMEN
The implementation of precursor management can improve safety performance of construction projects through effectively managing the correlations between construction accidents and their precursors. However, a system of comprehensive knowledge about what precursors mean within the context of construction safety is still lacking. This study aims to capture the nature of precursors in the construction industry and explore the process of a precursor event evolving into a construction accident to fill this gap. Based on 135 construction accident reports in China, this study adopts grounded theory to identify different types of accident precursors and explore their interactions with the development of the accident. An indicator system of precursors for construction accidents was developed, which included two major categories of precursors: behavioral factors and physical factors and five minor categories of precursors: individual behavior factors, organizational driving factors, objective physical factors, construction environmental factors, mechanical equipment factors. In addition, a precursor management strategy that includes the three stages of identification, response and effectiveness testing was established. The results of the study reveal the correlations between precursors and construction accidents, which can promote construction professionals' better understanding about precursors and improve their capabilities of managing precursors in practice.
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Industria de la Construcción , Administración de la Seguridad , Accidentes , Accidentes de Trabajo , China , Teoría FundamentadaRESUMEN
Antibodies play essential roles in both diagnostics and therapeutics. Epitope mapping is essential to understand how an antibody works and to protect intellectual property. Given the millions of antibodies for which epitope information is lacking, there is a need for high-throughput epitope mapping. To address this, we developed a strategy, Antibody binding epitope Mapping (AbMap), by combining a phage displayed peptide library with next-generation sequencing. Using AbMap, profiles of the peptides bound by 202 antibodies were determined in a single test, and linear epitopes were identified for >50% of the antibodies. Using spike protein (S1 and S2)-enriched antibodies from the convalescent serum of one COVID-19 patient as the input, both linear and potentially conformational epitopes of spike protein specific antibodies were identified. We defined peptide-binding profile of an antibody as the binding capacity (BiC). Conceptually, the BiC could serve as a systematic and functional descriptor of any antibody. Requiring at least one order of magnitude less time and money to map linear epitopes than traditional technologies, AbMap allows for high-throughput epitope mapping and creates many possibilities.
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COVID-19/inmunología , Mapeo Epitopo/métodos , Glicoproteína de la Espiga del Coronavirus/inmunología , Anticuerpos Monoclonales/inmunología , Anticuerpos Monoclonales/metabolismo , Anticuerpos Antivirales/metabolismo , Ensayo de Inmunoadsorción Enzimática , Epítopos/metabolismo , Proteínas de Escherichia coli/inmunología , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Sueros Inmunes/sangre , Sueros Inmunes/inmunología , Biblioteca de PéptidosAsunto(s)
Anticuerpos Neutralizantes/química , Anticuerpos Antivirales/química , Linfocitos B/inmunología , Betacoronavirus/inmunología , Infecciones por Coronavirus/inmunología , Epítopos/química , Neumonía Viral/inmunología , Glicoproteína de la Espiga del Coronavirus/química , Anticuerpos Neutralizantes/genética , Anticuerpos Neutralizantes/inmunología , Anticuerpos Antivirales/genética , Anticuerpos Antivirales/inmunología , Linfocitos B/virología , Betacoronavirus/genética , Betacoronavirus/patogenicidad , Sitios de Unión , COVID-19 , Convalecencia , Infecciones por Coronavirus/virología , Mapeo Epitopo , Epítopos/genética , Epítopos/inmunología , Expresión Génica , Interacciones Huésped-Patógeno/genética , Interacciones Huésped-Patógeno/inmunología , Humanos , Sueros Inmunes/química , Inmunoconjugados/química , Inmunoconjugados/genética , Inmunoconjugados/inmunología , Modelos Moleculares , Pruebas de Neutralización , Pandemias , Neumonía Viral/virología , Análisis por Matrices de Proteínas , Unión Proteica , Conformación Proteica en Hélice alfa , Conformación Proteica en Lámina beta , Dominios y Motivos de Interacción de Proteínas , SARS-CoV-2 , Glicoproteína de la Espiga del Coronavirus/genética , Glicoproteína de la Espiga del Coronavirus/inmunologíaAsunto(s)
Betacoronavirus/genética , Interacciones Huésped-Patógeno/genética , Interferón beta/genética , Proteínas de Transporte de Membrana Mitocondrial/genética , Proteínas Virales/genética , Secuencia de Aminoácidos , Betacoronavirus/metabolismo , Betacoronavirus/patogenicidad , Regulación de la Expresión Génica , Células HEK293 , Interacciones Huésped-Patógeno/inmunología , Humanos , Interferón beta/inmunología , Mitocondrias/metabolismo , Mitocondrias/virología , Proteínas de Transporte de Membrana Mitocondrial/inmunología , Proteínas del Complejo de Importación de Proteínas Precursoras Mitocondriales , Sistemas de Lectura Abierta , Regiones Promotoras Genéticas , Unión Proteica , Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo/genética , Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo/metabolismo , Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo/patogenicidad , SARS-CoV-2 , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Transducción de Señal , Proteínas Virales/metabolismoRESUMEN
BACKGROUND: Ginseng is one of the well-known medicinal plants, exhibiting diverse medicinal effects. Its roots possess anticancer and antiaging properties and are being used in the medical systems of East Asian countries. It is grown in low-light and low-temperature conditions, and its growth is strongly inhibited at temperatures above 25°C. However, the molecular responses of ginseng to heat stress are currently poorly understood, especially at the protein level. METHODS: We used a shotgun proteomics approach to investigate the effect of heat stress on ginseng leaves. We monitored their photosynthetic efficiency to confirm physiological responses to a high-temperature stress. RESULTS: The results showed a reduction in photosynthetic efficiency on heat treatment (35°C) starting at 48 h. Label-free quantitative proteome analysis led to the identification of 3,332 proteins, of which 847 were differentially modulated in response to heat stress. The MapMan analysis showed that the proteins with increased abundance were mainly associated with antioxidant and translation-regulating activities, whereas the proteins related to the receptor and structural-binding activities exhibited decreased abundance. Several other proteins including chaperones, G-proteins, calcium-signaling proteins, transcription factors, and transfer/carrier proteins were specifically downregulated. CONCLUSION: These results increase our understanding of heat stress responses in the leaves of ginseng at the protein level, for the first time providing a resource for the scientific community.
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Biotransformation of Echinocystic acid (EA,1) using G. roseum CGMCC 3.3657 has been investigated, which leads to the isolation and identification of two novel Echinocystic acid derivatives, 4, 16α-dihydroxy-3,4-seco-olean-12-en-3,28-dioic acid (2) and 16α-hydroxy, A-homo-3α-oxa-olean-12-en-3-one-28-oic acid (3). Their structures have been elucidated by analysis of spectroscopic data. This biocatalysis could serve as an efficient tool complementary to classical chemical methods for the transformation of EA.
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Biotransformación , Gliocladium/metabolismo , Ácido Oleanólico/análogos & derivados , Catálisis , Estructura Molecular , Ácido Oleanólico/química , Análisis EspectralRESUMEN
Donkeys (Equus asinus) are widely distributed throughout China; they are used for their meat, as food, and certain donkey-derived items are also important for traditional Chinese medicinal purposes. However, only limited information is available on Toxoplasma gondii infection in donkeys in China, especially the eastern region, which is one of the largest production areas. Thus, the present study was conducted to detect specific anti-T. gondii antibodies using a commercially available indirect hemagglutination test (IHA) kit and to evaluate the risk factors that are associated with seroprevalence in the Shandong province of eastern China. A total of 213/1278 (17%) donkeys tested from Shandong province were positive for T. gondii antibodies. Statistical analysis revealed that gender and feeding habits of the animal are associated with T. gondii infection. These results provide information for the prevention and control of toxoplasmosis in donkeys, other animals, and humans in this region and elsewhere.
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Equidae/parasitología , Toxoplasma/inmunología , Toxoplasmosis Animal/epidemiología , Animales , Anticuerpos Antiprotozoarios/sangre , China/epidemiología , Equidae/inmunología , Femenino , Pruebas de Hemaglutinación/veterinaria , Humanos , Masculino , Carne/parasitología , Factores de Riesgo , Estudios Seroepidemiológicos , Toxoplasmosis Animal/inmunología , Toxoplasmosis Animal/transmisiónRESUMEN
Ungulate bocaparvoviruses (UBoV) 2-5 are recently discovered porcine bocaparvoviruses belonging to the family Parvoviridae, and are considered to be a potentially major cause of swine diseases. In order to detect local UBoV2 epidemics in China, we developed a TaqMan-based real-time PCR (qPCR) assay targeting the UBoV2 VP1 gene and compared the results of qPCR with conventional PCR (cPCR). The qPCR reproducibly detected a recombinant DNA plasmid containing the VP1 gene over a range of eight orders of magnitude, from 9.97 × 10-1-106 copies/µL, with a lower limit of detection of 9.97 copies/µL, compared with approximately 9.97 × 102 copies/µL for cPCR. The qPCR assay showed no cross-reactivity with other UBoVs or other porcine viruses. This qPCR assay detected UBoV2 in 18.1% (84/463) of pig samples collected from Chinese swine herds, with the highest infection rate of 35.3% (53/150) in loose stools. UBoV2 was not detected in liver samples. The TaqMan-based qPCR assay established in this study was highly sensitive and specific for the diagnosis and quantification of UBoV2. The results of this study will further our understanding of the etiology, epidemiology, and pathogenesis of UBoV2 infection.
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Bocavirus/aislamiento & purificación , Infecciones por Parvoviridae/veterinaria , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Enfermedades de los Porcinos/diagnóstico , Animales , Bocavirus/genética , China , Heces/virología , Infecciones por Parvoviridae/virología , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Porcinos , Enfermedades de los Porcinos/virología , Carga Viral/métodosRESUMEN
BACKGROUND: Pet ownership in China has been steadily increasing over recent years. However, the risk of pet-associated zoonotic infection with the protozoan parasite Toxoplasma gondii remains poorly defined. METHODS: In a cross-sectional survey, we have determined the seroprevalence of T. gondii infection in pet dogs and cats, and pet owners. Serum samples were collected from 360 pets and 460 corresponding pet owners between March 2016 to June 2017, from Shandong province, eastern China. Sera from the animals were tested for anti-T. gondii antibodies using an indirect haemagglutination assay (IHA) and from the pet owners using an enzyme-linked immunosorbent assay (ELISA). RESULTS: Antibodies against T. gondii were detected in 67 of 360 (18.61%) pets. Seroprevalence of T. gondii in pet cats and dogs was 21.67% and 15.56%, respectively. IgG and IgM antibodies were detected in 79 (17.17%) and 4 (0.87%) of pet owners, respectively; with a total of 83 of 460 (18.04%) pet owners testing seropositive for T. gondii. Our seroprevalence data also suggest that cat owners in general and female pet owners in particular could face a higher risk of acquiring T. gondii infection. CONCLUSIONS: Significant levels of anti-T. gondii antibodies were detected in the pets and their owners in Shandong province, eastern China, indicating a potential zoonotic risk. Prophylactic measures should be implemented to reduce the risk of pet owner's exposure to T. gondii infection.
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Anticuerpos Antiprotozoarios/sangre , Mascotas/sangre , Toxoplasma/inmunología , Toxoplasmosis Animal/sangre , Toxoplasmosis Animal/epidemiología , Toxoplasmosis/sangre , Toxoplasmosis/epidemiología , Adulto , Anciano , Anciano de 80 o más Años , Animales , Gatos/sangre , Gatos/inmunología , China/epidemiología , Estudios Transversales , Perros/sangre , Perros/inmunología , Perros/parasitología , Ensayo de Inmunoadsorción Enzimática , Femenino , Vínculo Humano-Animal , Humanos , Masculino , Persona de Mediana Edad , Mascotas/inmunología , Mascotas/parasitología , Prevalencia , Estudios Seroepidemiológicos , Toxoplasmosis/inmunología , Toxoplasmosis Animal/inmunología , Adulto Joven , Zoonosis/sangre , Zoonosis/epidemiología , Zoonosis/inmunologíaRESUMEN
Microsporidiosis is an emerging and opportunistic disease, and Enterocytozoon bieneusi is the main cause of this disease in humans. Little information is available on prevalence and genotyping of E. bieneusi in minks. We collected 559 feces samples of minks from Heilongjiang and Jilin provinces in 2017, and studied E. bieneusi prevalence by nested PCR. A total of 23 out of 559 minks (4.1%) were detected as E. bieneusi-positive, and were raised in five of the seven investigated farms. Age was the only risk factor associated with E. bieneusi prevalence in investigated minks through logistic regression analysis. Sequence analysis of the ITS gene revealed that five E. bieneusi ITS genotypes, including Peru11, EbpC, and three novel genotypes (HLJM-1, HLJM-2 and JLM-1) were present, suggesting minks may be a potential source of human microsporidiosis.
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Enterocytozoon/genética , Genotipo , Microsporidiosis/veterinaria , Visón/microbiología , Factores de Edad , Animales , China/epidemiología , ADN de Hongos/genética , ADN Espaciador Ribosómico/genética , Enterocytozoon/clasificación , Heces/microbiología , Femenino , Masculino , Microsporidiosis/epidemiología , Microsporidiosis/microbiología , Filogenia , Reacción en Cadena de la Polimerasa , Prevalencia , Análisis de Secuencia de ADNRESUMEN
Currently, there is no information available on the detection of Toxoplasma gondii and Neospora caninum in the tissues of Tolai hares in China. This study aimed to investigate the prevalence of these protozoan parasites in Tolai hares obtained from Shandong province, eastern China, between January 2016 and June 2017. Serum and brain tissue samples of 358 Tolai hares were obtained and detected for the presence of antibody and parasite DNAs by serodiagnosis and polymerase chain reaction (PCR), respectively. The seroprevalence of T. gondii and N. caninum infection in Tolai hares was 8.10% (29/358) and 0.84% (3/358), respectively. However, all the 358 tested Tolai hares were negative for N. caninum by PCR and T. gondii DNA was detected in 23 Tolai hares (6.42%, 23/358). The positive T. gondii DNA was genotyped at 11 genetic markers using multilocus PCR-restriction fragment length polymorphism technology. Of the 23 positive samples, only 2 of them produced complete genotyping results, and were identified as ToxoDB Genotype #9. This is the first report to detect T. gondii in the tissues of Tolai hares from China and the first study to focus on N. caninum in Tolai hares from China.
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Liebres/parasitología , Neospora/genética , Toxoplasma/genética , Toxoplasmosis Animal/epidemiología , Animales , Anticuerpos Antiprotozoarios/sangre , China/epidemiología , Coccidiosis/parasitología , ADN Protozoario/análisis , Femenino , Inocuidad de los Alimentos , Genotipo , Liebres/sangre , Masculino , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de RestricciónRESUMEN
This study was conducted to detect specific anti-Neospora antibodies using a commercial competitive-inhibition ELISA kit, and to evaluate the risk factors for Neospora spp. infection. Out of a total of 2,228 donkey sera collected in three provinces in China, 211 (9.5%) were found to be positive for anti-Neospora antibodies. Statistical analysis revealed that age (p = 0.019, OR = 1.62, 95%CI: 1.08-2.44), feeding status (p < 0.001, OR = 3.79, 95%CI: 2.65-5.43), miscarriage history (p = 0.006, OR = 2.56, 95%CI: 1.27-4.01), and contact with dogs (p < 0.001, OR = 2.69, 95%CI: 1.86-3.88) were significant risk factors for Neospora spp. infection. This is the first evidence of Neospora infection in donkeys in China.
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Anticuerpos Antiprotozoarios/sangre , Coccidiosis/veterinaria , Equidae/parasitología , Neospora/inmunología , Aborto Veterinario/epidemiología , Aborto Veterinario/parasitología , Factores de Edad , Animales , China/epidemiología , Coccidiosis/epidemiología , Perros , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Modelos Logísticos , Masculino , Análisis Multivariante , Juego de Reactivos para Diagnóstico/veterinaria , Factores de Riesgo , Estudios SeroepidemiológicosRESUMEN
Until now, limited information on the molecular epidemiology and genotypes of Toxoplasma gondii infection in donkeys is available in China. Thus, the present study was conducted to characterize T. gondii genotypes in donkeys, intended for human consumption in Shandong province, eastern China. A total of 618 muscle tissue samples of donkeys was collected in Shandong province from January 2016 to August 2017 and were used to detect the T. gondii B1 gene by a semi-nested PCR, and the positive samples were genotyped at 10 nuclear loci (i.e., SAG1, alternative SAG2, 5'-and 3'-SAG2, SAG3, L358, BTUB, c22-8, GRA6, c29-2, PK1) and an apicoplast locus Apico by multi-locus PCR-RFLP method. Fifty-seven (9.22%) samples out of 618 donkey meat samples were examined T. gondii B1 gene positive. In this study, no risk factor was found to be associated with T. gondii infection in donkeys. Moreover, two genotypes (ToxoDB#9 and ToxoDB#1) were identified. This is the first genetic characterization of T. gondii isolated from donkey meat that would intend for human consumption in Shandong province, eastern China and also the first report of genotype ToxoDB#1 found in donkeys in China, which may be useful for preventing and controlling T. gondii infection in donkeys, other animals and humans.