RESUMEN
We studied the molecular mechanisms of cross-adaptation to ionizing radiation (1 Gy) of lymphocytes isolated from rats subjected to emotional stress. The effects of chronic (CES; various types of stress exposure) and acute (AES; forced swimming) emotional stress in rats on indicators of oxidative stress, cell death, and levels of NRF2 and NOX4 proteins involved in the development of the adaptive response were analyzed in isolated lymphocytes. It was found that stress induced an adaptive response in rat lymphocytes and triggered processes similar to the adaptive response induced by low doses of ionizing radiation: an increase in the level of oxidized DNA and cell death, as well as an increase in the content of NOX4 and NRF2 proteins. In animals subjected to emotional stress, suppressed DNA oxidation in response to irradiation, reduced levels of protective factor NRF2, as well as lymphocyte death were observed.
Asunto(s)
Linfocitos , Factor 2 Relacionado con NF-E2 , Estrés Oxidativo , Radiación Ionizante , Estrés Psicológico , Animales , Linfocitos/efectos de la radiación , Linfocitos/metabolismo , Ratas , Factor 2 Relacionado con NF-E2/metabolismo , Factor 2 Relacionado con NF-E2/genética , Estrés Psicológico/metabolismo , Masculino , Estrés Oxidativo/efectos de la radiación , Ratas Wistar , Adaptación Fisiológica/efectos de la radiación , NADPH Oxidasa 4/metabolismo , NADPH Oxidasa 4/genética , Daño del ADN/efectos de la radiaciónRESUMEN
The authors presented results of hygienic evaluation of various techonologies connected with use of fipronil-based insecticides. Findings are allowable risk of fipronil exposure in workers, if technologic regulations and safety rules met. Exceeded allowable risk in some technologies is due to technologic process features, outdated equipment and poor efficiency of individual protective means - that requires management decisions.