Long-Period Gratings and Microcavity In-Line Mach Zehnder Interferometers as Highly Sensitive Optical Fiber Platforms for Bacteria Sensing.

Selected optical fiber sensors offer extraordinary sensitivity to changes in external refractive (RI), which make them promising for label-free biosensing. In this work the most sensitive ones, namely long-period gratings working at (DTP-LPG) and micro-cavity in-line Mach-Zehnder interferometers (µIMZI) are discussed for application in bacteria sensing. We describe their working principles and RI sensitivity when operating in water environments, which is as high as 20,000 nm/RIU (Refractive index unit) for DTP-LPGs and 27,000 nm/RIU for µIMZIs. Special attention is paid to the methods to enhance the sensitivity by etching and nano-coatings. While the DTP-LPGs offer a greater interaction length and sensitivity to changes taking place at their surface, the µIMZIs are best suited for investigations of sub-nanoliter and picoliter volumes. The capabilities of both the platforms for bacteria sensing are presented and compared for strains of Escherichia coli, lipopolysaccharide E. coli, outer membrane proteins of E. coli, and Staphylococcus aureus. While DTP-LPGs have been more explored for bacteria detection in 102-106 Colony Forming Unit (CFU)/mL for S. aureus and 103-109 CFU/mL for E. coli, the µIMZIs reached 102-108 CFU/mL for E. coli and have a potential for becoming picoliter bacteria sensors.

Dataset of MAPLE Parameters for Hemoglobin Deposition upon long period gratings.

Matrix-assisted pulsed laser evaporation (MAPLE) is an alternative and complimentary method to pulsed laser deposition. MAPLE has been demonstrated to be a less harmful approach for transporting and depositing delicate, highly sensitive molecules. Metalloproteins are considered sensitive molecules since their bioactivity is determined not only by their chemical structure but also by conformational changes that can be altered by deposition methods. Here we report a dataset of MAPLE deposition parameters of haemoglobin (Hb) that ensures the retention of its bioactivity. Methods for parameters optimization are also described. The data and analysis should be valuable for researchers interested in application of MAPLE techniques for metalloprotein immobilization since it provides a unique opportunity for direct immobilization. The data presents the results of previously conducted experiments on the basis of which is based the research article entitled "A Highly Efficient Biosensor based on MAPLE Deposited Hemoglobin on LPGs Around Phase Matching Turning Point" [1].

Combined Long-Period Fiber Grating and Microcavity In-Line Mach-Zehnder Interferometer for Refractive Index Measurements with Limited Cross-Sensitivity.

This work discusses sensing properties of a long-period grating (LPG) and microcavity in-line Mach-Zehnder interferometer (µIMZI) when both are induced in the same single-mode optical fiber. LPGs were either etched or nanocoated with aluminum oxide (Al2O3) to increase its refractive index (RI) sensitivity up to ≈2000 and 9000 nm/RIU, respectively. The µIMZI was machined using a femtosecond laser as a cylindrical cavity (d = 60 µm) in the center of the LPG. In transmission measurements for various RI in the cavity and around the LPG we observed two effects coming from the two independently working sensors. This dual operation had no significant impact on either of the devices in terms of their functional properties, especially in a lower RI range. Moreover, due to the properties of combined sensors two major effects can be distinguished-sensitivity to the RI of the volume and sensitivity to the RI at the surface. Considering also the negligible temperature sensitivity of the µIMZI, it makes the combination of LPG and µIMZI sensors a promising approach to limit cross-sensitivity or tackle simultaneous measurements of multiple effects with high efficiency and reliability.

Immunosensor Based on Long-Period Fiber Gratings for Detection of Viruses Causing Gastroenteritis.

Since the norovirus is the main cause of acute gastroenteritis all over the world, its fast detection is crucial in medical diagnostics. In this work, a rapid, sensitive, and selective optical fiber biosensor for the detection of norovirus virus-like particles (VLPs) is reported. The sensor is based on highly sensitive long-period fiber gratings (LPFGs) coated with antibodies against the main coat protein of the norovirus. Several modification methods were verified to obtain reliable immobilization of protein receptors on the LPFG surface. We were able to detect 1 ng/mL norovirus VLPs in a 40-min assay in a label-free manner. Thanks to the application of an optical fiber as the sensor, there is a possibility to increase the user's safety by separating the measurement point from the signal processing setup. Moreover, our sensor is small and light, and the proposed assay is straightforward. The designed LPFG-based biosensor could be applied in both fast norovirus detection and in vaccine testing.

Label-free cocaine aptasensor based on a long-period fiber grating.

In this Letter, we combined a promising bioreceptor, a cocaine aptamer MN6, with an ultrasensitive optical platform long-period fiber grating (LPFG) to create a new cocaine biosensor. The cocaine induces a conformational rearrangement of the aptamer which changes the refractive index around the LPFG producing a measurable shift of the transmission spectrum. We were able to track subtle interaction between the receptor and cocaine molecules over a concentration range of 25 to 100 µM. The presented biosensor does not require labeling or signal enhancement, resulting in a simple user-friendly device.

Ambient Refractive-Index Measurement with Simultaneous Temperature Monitoring Based on a Dual-Resonance Long-Period Grating Inside a Fiber Loop Mirror Structure.

In this work, we report the experimental results on optimizing the optical structure for ambient refractive index measuring with temperature changes monitoring. The presented optical structure is based on a dual-resonance long-period grating embedded inside a fiber loop mirror, where the long-period grating acts as the head of the refractive-index sensor, whereas the section of polarization maintaining fiber in the loop mirror ensures suitable temperature sensing. The optimization process was comprised of tuning the resonance and interferometric peaks by changing the state of polarization of propagating beams. Experimental results establish that the response of the proposed sensor structure is linear and goes in opposite directions: an increase in the ambient refractive index reduces the signal response, whereas a temperature increase produces an increased response. This enables us to distinguish between the signals from changes in the refractive index and temperature. Due to the filtering properties of the interferometric structure, it is possible to monitor variation in these physical parameters by observing optical power changes instead of wavelength shifts. Hence, the refractive index sensitivity has been established up to 2375.8 dB/RIU in the narrow RI range (1.333⁻1.341 RIU) and temperature sensitivities up to 1.1 dBm/°C in the range of 23⁻41 °C. The proposed sensor is dedicated to advanced chemical and biological sensor applications.

Compact and cost-effective temperature-insensitive bio-sensor based on long-period fiber gratings for accurate detection of E. coli bacteria in water.

We propose and demonstrate a novel temperature-insensitive bio-sensor for accurate and quantitative detection of Escherichia coli (E. coli) bacteria in water. Surface sensitivity is maximized by operating the long-period fiber grating (LPFG) closest to its turnaround wavelength, and the temperature insensitivity is achieved by selectively exciting a pair of cladding modes with opposite dispersion characteristics. Our sensor shows a nominal temperature sensitivity of ∼1.25 pm/°C, which can be further reduced by properly adjusting the LPFG lengths, while maintaining a high refractive index sensitivity of 1929 nm/RIU. The overall length of the sensor is ∼3.6 cm, making it ideally suitable for bio-sensing applications. As an example, we also show the sensor's capability for reliable, quantitative detection of E. coli bacteria in water over a temperature fluctuation of room temperature to 40°C.

Towards refractive index sensitivity of long-period gratings at level of tens of µm per refractive index unit: fiber cladding etching and nano-coating deposition.

In this work we report experimental results on optimizing the refractive index (RI) sensitivity of long-period gratings (LPGs) by fiber cladding etching and thin aluminum oxide (Al2O3) overlay deposition. The presented LPG takes advantage of work in the dispersion turning point (DTP) regime as well as the mode transition (MT) effect for higher-order cladding modes (LP09 and LP010). The MT was obtained by depositing Al2O3 overlays with single-nanometer precision using the Atomic Layer Deposition method (ALD). Etching of both the overlay and the fiber cladding was performed using hydrofluoric acid (HF). For shallow etching of the cladding, i.e., DTP observed at next = 1.429 and 1.439 RIU for an LPG with no overlay, followed by deposition of an overlay of up to 167 nm in thickness, HF etching allowed for post-deposition fine-tuning of the overlay thickness resulting in a significant increase in RI sensitivity mainly at the DTP of the LP09 cladding mode. However, at an external RI (next) above 1.39 RIU, the DTP of LP010 was noticed, and its RI sensitivity exceeded 9,000 nm/RIU. Deeper etching of the cladding, i.e., DTP observed for next above 1.45 RIU, followed by the deposition of thicker overlays (up to 201 nm in thickness) allowed the sensitivity to reach values of over 40,000 nm/RIU in a narrow RI range. Sensitivity exceeding 20,000 nm/RIU was obtained in an RI range suitable for label-free biosensing applications.

Improving the electric field sensing capabilities of the long-period fiber grating coated with a liquid crystal layer.

The hybrid liquid crystal long-period fiber grating structure presented here uses the 1702 liquid crystal as a thin layer on the bare long-period fiber grating. To achieve the highest long-period fiber grating sensitivity to the liquid crystal layer presence, a UV-induced host grating, with a relatively short period of 226.8 µm, was chosen. This design makes it possible to couple light from the propagating core mode to a cladding mode at a wavelength near the phase-matching turning point. This phenomenon is exploited here for the first time to measure the thermal and electric field responses of the liquid crystal long-period fiber grating structure. All experimental results achieved in this work are supported by theoretical analysis.

Label-free sensitivity of long-period gratings enhanced by atomic layer deposited TiO(2) nano-overlays.

In this paper, we discuss an impact of thin titanium dioxide (TiO(2)) coatings on refractive index (RI) sensitivity and biofunctionalization of long-period gratings (LPGs). The TiO(2) overlays on the LPG surfaces have been obtained using atomic layer deposition (ALD) method. This method allows for a deposition of conformal, thickness-controlled, with well-defined optical properties, and high-RI thin films which are highly desired for optical fiber sensors. It has been found that for LPGs working at a dispersion turning point of higher order cladding modes only tens of nanometers of TiO(2) overlay thickness allow to obtain cladding mode transition effect, and thus significant improvement of RI sensitivity. When the TiO(2) overlay thickness reaches 70 nm, it is possible to obtain RI sensitivity exceeding 6200 nm/RIU in RI range where label-free sensors operate. Moreover, LPGs with TiO(2)-enhanced RI sensitivity have shown improved sensitivity to bacteria endotoxin (E. coli B lipopolysaccharide) detection, when TiO(2) surface is functionalized with endotoxin binding protein (adhesin) of T4 bacteriophage.

Measurements of reactive ion etching process effect using long-period fiber gratings.

The paper presents for the first time a study of long-period fiber gratings (LPFGs) applied for the measurements of reactive ion etching (RIE) process effect in various places of a plasma reactor. For the purposes of the experiment a number of highly sensitive LPFGs working at the dispersion turning point was fabricated using electric arc discharges. We show that the LPFGs allow for monitoring of the phenomena taking place in the reactor, especially those resulting in reduction of the LPFG diameter. Results of the measurements supported by simulations have shown that etching rate significantly decreases with elevation of the sample up to 3.6 mm over the electrode in the reactor, and stays constant above this height.

Temperature insensitive high-precision refractive-index sensor using two concatenated dual-resonance long-period gratings.

In this Letter we report on fabricating and analyzing a temperature insensitive refractometer based on two concatenated dual-resonance long-period gratings (LPGs) with an appropriate inter-grating space (IGS) in between. The IGS provides a temperature-dependent extra phase difference between the core and cladding modes, making the refractometer similar to a Mach-Zehnder interferometer with its arms phase shifted. We demonstrate that an appropriate IGS can produce temperature-insensitive resonance wavelengths. The interferometer is highly stable over a wide range of temperature (20°C-100°C). The measured refractive index sensitivity for aqueous solutions (1.333-1.343) is ~2583 nm/RIU, which is the highest reported so far for biological samples. The interferometer can be used for various other temperature-immune sensing applications also.

Long period grating based biosensor for the detection of Escherichia coli bacteria.

In this paper we report a stable, label-free, bacteriophage-based detection of Escherichia coli (E. coli) using ultra sensitive long-period fiber gratings (LPFGs). Bacteriophage T4 was covalently immobilized on optical fiber surface and the E. coli binding was investigated using the highly accurate spectral interrogation mechanism. In contrast to the widely used surface plasmon resonance (SPR) based sensors, no moving part or metal deposition is required in our sensor, making the present sensor extremely accurate, very compact and cost effective. We demonstrated that our detection mechanism is capable of reliable detection of E. coli concentrations as low as 10(3)cfu/ml with an experimental accuracy greater than 99%.

Detection of bacteria using bacteriophages as recognition elements immobilized on long-period fiber gratings.

The paper presents for the first time a study of long-period gratings (LPGs) applied for label-free detection of specific bacteria using physically adsorbed bacteriophages. For the purposes of the experiment a number of highly sensitive LPGs working at the turning point of phase matching curve was fabricated in SMF28 fiber using UV exposure. We show that the device allows for real-time monitoring of phenomena taking place on the sensor's surface, including phage-bacteria interactions. For the applied conditions a resonance wavelength shift of ~1.3 nm induced by bacteria binding was observed.

Pressure sensing in high-refractive-index liquids using long-period gratings nanocoated with silicon nitride.

The paper presents a novel pressure sensor based on a silicon nitride (SiNx) nanocoated long-period grating (LPG). The high-temperature, radio-frequency plasma-enhanced chemical-vapor-deposited (RF PECVD) SiNx nanocoating was applied to tune the sensitivity of the LPG to the external refractive index. The technique allows for deposition of good quality, hard and wear-resistant nanofilms as required for optical sensors. Thanks to the SiNx nanocoating it is possible to overcome a limitation of working in the external-refractive-index range, which for a bare fiber cannot be close to that of the cladding. The nanocoated LPG-based sensing structure we developed is functional in high-refractive-index liquids (nD>1.46) such as oil or gasoline, with pressure sensitivity as high as when water is used as a working liquid. The nanocoating developed for this experiment not only has the highest refractive index ever achieved in LPGs (n>2.2 at λ=1,550 nm), but is also the thinnest (<100 nm) able to tune the external-refractive-index sensitivity of the gratings. To the best of our knowledge, this is the first time a nanocoating has been applied on LPGs that is able to simultaneously tune the refractive-index sensitivity and to enable measurements of other parameters.