[The effect of local anesthetics on the electric properties of black flat bilayers]. / Vliianie mestnykh anestetikov na élektricheskie svoistva ploskikh chernykh bisloev.

The effect of some local anesthetics on conductance of bilayer lipid membranes made of egg yolk lecithin has been studied. Low concentrations of anesthetics were shown to gradually increase the integral conductance of bilayers. This effect is connected with the protonophore activity of the tested anesthetics and it caused the stabilization of the bilayers. High concentrations of anesthetics caused destabilization of membranes and formed zones of nonselective increased conductance.

[Thermotropic behavior of liposomes from egg yolk lecithin, hydrogenized egg yolk lecithin and their mixtures]. / Termotropnoe povedenie liposom iz iaichnogo letsitina i gidrogenizirovannogo iaichnogo letsitina i ikh smesei.

The thermotropic properties of multilamellar liposomes from egg yolk lecithin, hydrogenized egg yolk lecithin and several mixtures of these two lipids were studied with the application of excimer--forming optical probe pyrene and microcalorimetry. It was discovered that when the proportion of the egg yolk lecithin in the lipid mixture was raised the temperature of the main phase transition reduced. For all this, independent of the lipid mixture composition when the temperature was raised, apparently, polarity of pyrene microenvironment in the liposomes bilayers decreased. On the basis of the analysis of solidus and liquidus curves obtained from calorimetric studies of the lipid mixtures and bend points of Arrhenius anamorphose obtained during the pyrene excimer formation measurements some conclusions were made about the role of unmodified and hydrogenized egg yolk lecithin cluster formation in the determination of thermotropic properties of the liposomes from the above two lipids mixtures. High temperature phase transition discovered for the egg yolk lecithin while measuring the pyrene excimer formation is proposed to be closely connected with temperature-dependent changes in the organization of phospholipid heads on the interphase bilayer/H2O solution.

[The action of gramicidin S on the ionic permeability of bilayer lipid membranes]. / Deistvie gramitsidina S na ionnuiu pronitsaemost' bisloinykh lipidnykh membran.

The effect of cyclic decapeptide of gramicidin S on electrical conductivity of bilayer lipid membranes has been studied. The integral conductivity of bilayer has been shown to increase with the growth of antibiotic concentration. The integral conductivity increase occurs as series of conductivity discrete leaps, differing in amplitude from fluctuations of conductivity caused by linear gramicidins. In the series of selectivity of bilayer membranes for cations of alkaline metals the rubidium ion is before the cesium ion. This is the only difference between this series and the series of relative ionic mobility series of cations of alkaline metals in water solutions.

[Effect of the C35 analog of dolichol on the electrical properties of planar black bilayers]. / Vliianie C35-analoga dolikhola na élektricheskie svoistva ploskikh chernykh sloev.

The effect of C35-dolichol on black planar bilayers from mixture of azolectin and cholesterol has been studied. C35-dolichol in concentration of about 10(-6) g/ml has been found to raise the bilayer conductivity, inducing current jumps with unclearly expressed conductivity level of 0.4-0.5 nSm. According to the data of the first and third harmonic during the several minutes after C35-dolichol addition along one side of the bilayer the capacity of the membranes and transversal module Young increase. It is supposed that dolichols induce the nonbilayer structure in membranes that is the basis for their transfer of oligosaccharides blocks across the membranes of Golgi apparatus.

[Current fluctuations across the bilayer membrane in the presence of prostaglandin F2 alpha]. / Tokovye fluktuatsii cherez bisloinuiu membranu v prisutstvii prostaglandina F2 alpha.

The prostaglandin F2 alpha (in concentration 1,4 or 14 microM) has been shown to induce the current fluctuations across the bilayers under the action on the planar bilayer membranes from the soya total phospholipids. The current fluctuations depend on the potential, their number expresses indistinctly and corresponds to the conductivity jumps across the bilayer about 5 pSm.

[Thermotropic changes in the conductivity of black bilayers from egg phosphatidylethanolamine]. / Termotropnye izmeneniia provodimosti chernykh bisloev iz iaichnogo fosfatidilétanolamina.

Current fluctuations in black bilayers from phosphatidyl ethanolamine obtained from egg lecithin were registered in the temperature region of the main phase transition of this phospholipid and the bilayer--hexagonal phase transition about 35 degrees; they correspond to the conductivity changes of hundreds of pSm. This transition takes place in the same temperature region as shown by 31P-NMR and depolarization of light-scattering method in phosphatidyl ethanolamine multilamellar liposomes. The scheme of bilayer transformation into hexagonal phase in the temperature region of lipid polymorphic transition is discussed.

[Use of bromthymol blue for studying the thermotropic properties of the liposomes from egg lecithin]. / Primenenie bromtimolovogo sinego dlia issledovaniia termotropnykh svoistv liposom iz iachnnogo letsitina.

The dependence of the turbidity changes at 615 nm monobilayers liposomes from egg yolk lecithin in the presence of bromothymol blue on temperature and storage conditions has been investigated. It is established that the thermotropic properties of liposomes change irregularly and depend on the storage conditions. Sharp release of the bound dye at temperature above 35-37 degrees C is associated with thermotropic change in liposomes and the detected effects, with the change of orientation of the phosphorylcholine group.