RESUMEN
PURPOSE: To measure the central corneal thickness (CCT) using anterior segment optical coherence tomography (AS-OCT) in older adults with and without pterygium from the Brazilian Amazon Region Eye Survey (BARES). METHODS: BARES is a population-based epidemiological cross-sectional study conducted in Parintins city. Participants were residents ≥45 years of age identified through a door-to-door interview. Eligible participants were invited for a comprehensive eye exam. Pterygium occurrence and severity were assessed by ophthalmologists through slit-lamp examination considering its location (nasal or/and temporal) and severity (lesion with extension <3â mm, ≥3â mm not reaching the pupillary margin or ≥3â mm reaching the pupillary margin). CCTs were obtained and measurements from the more severely affected eye were included. Images were analyzed offline by masked observers. RESULTS: A total of 671 subjects, 533 (79.4%) with pterygium in at least one eye and 138 (20.6%) without pterygium in either eye, were examined. The mean CCT evaluated by multiple linear regression and adjusted for demographic variables and pterygium severity was 521 ± 34â µm (median = 521; range = 304-665). Decreased CCT was significantly associated with age and pterygium severity. Individuals aged 65-74 years had CCT 7â µm thinner than those aged 45-54 years (p = 0.044), individuals aged 75 years and older had CCT 15â µm thinner than those aged 45-54 years (p = 0.001), and eyes with severe pterygium had CCT 33â µm thinner than eyes without pterygium (p < 0.001). CONCLUSIONS: The CCT analysis in this population-based sample shows that a thinner cornea is associated with pterygium severity and older age.
Asunto(s)
Pterigion , Humanos , Anciano , Persona de Mediana Edad , Pterigion/diagnóstico , Pterigion/patología , Tomografía de Coherencia Óptica/métodos , Estudios Transversales , Córnea/patología , Reproducibilidad de los Resultados , Paquimetría Corneal/métodosRESUMEN
PURPOSE: To investigate the expression of specific receptors, signal transducers and the effect of transforming growth factor-beta (TGF-beta) on retinal pigment epithelium (RPE) migration and proliferation. METHODS: Human RPE cell line D407 was used in all experiments. The effect of TGF-beta on migration and proliferation were studied using a wound healing model and [3H]-thymidine incorporation, respectively. The expression of RNA related to the TGF-beta superfamily receptors and SMAD1-4 were assayed by reverse transcriptase-polymerase chain reaction (RT-CPR). The effects of TGF-beta on the intracellular position of SMAD were studied by immunoperoxidase and immunofluorescence. RESULTS: Transforming growth factor-beta 4 nm and activin A 0.36 nm stimulated RPE migration. There was no effect on proliferation. RNA for TGF-beta receptors types 1 and 2, and SMAD1-4 were detected in RPE culture. Transforming growth factor-beta signal transducer SMAD2 but not SMAD1 moved from the cytoplasm to the nucleus after TGF-beta stimulation. CONCLUSION: Transforming growth factor-beta can regulate RPE cell migration through specific signal transduction pathways.
Asunto(s)
Activinas/farmacología , Movimiento Celular/efectos de los fármacos , Subunidades beta de Inhibinas/farmacología , Epitelio Pigmentado Ocular/citología , Factor de Crecimiento Transformador beta/farmacología , Receptores de Activinas Tipo I/genética , Receptores de Activinas Tipo I/metabolismo , División Celular/efectos de los fármacos , Línea Celular , Núcleo Celular/metabolismo , Citoplasma/metabolismo , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Técnica del Anticuerpo Fluorescente Indirecta , Humanos , Técnicas para Inmunoenzimas , Epitelio Pigmentado Ocular/metabolismo , Proteínas Serina-Treonina Quinasas , ARN Mensajero/metabolismo , Receptor Tipo I de Factor de Crecimiento Transformador beta , Receptor Tipo II de Factor de Crecimiento Transformador beta , Receptores de Factores de Crecimiento Transformadores beta/genética , Receptores de Factores de Crecimiento Transformadores beta/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Proteínas Smad , Proteína Smad1 , Proteína Smad2 , Proteína smad3 , Proteína Smad4 , Transactivadores/genética , Transactivadores/metabolismo , Cicatrización de Heridas/fisiologíaRESUMEN
OBJETIVO A superfamília TGF-b é composta por diferentes proteínas multifuncionais como TGF-b, activina, proteína morfogenética óssea (BMP) que podem modular as funçoes das células do epitélio pigmentado da retina (EPR). No presente estudo, a expressao de receptores específicos e transdutores de sinal e o efeito do TGF-b sobre a migraçao e a proliferaçao do EPR foram observadas a fim de investigar a açao de membros da superfamília TGF-b sobre o EPR. MÉTODOS Culturas de linhagem de células do EPR humano, 13407, foram usadas em todos os experimentos. Os efeitos sobre a migraçao e proliferaçao celulares foram investigados pelo modelo de cicatrizaçao de ferida in vitro e pela incorporaçao de timidina tritiada, respectivamente. A expressao de mRNA relacionado aos receptores tipos I e II de TGF-b e aos mensageiros intracelulares (SMAD 1 a 4) foi investigada através da técnica de reaçao em cadeia da polimerase com transcriptase reversa (RT-PCR). A seguir, a açao do TGF-b sobre mensageiros intracelulares da família SMAD foi estudada através de imunofluorescência e imunoperoxidase. RESULTADOS A proliferaçao celular nao foi afetada pelo TGF-b1, enquanto a migraçao celular foi estimulada pelo TGF-b a 4nM e pela activina A a o,36 nM. A linhagem de células D407 expressou mRNA referente à transcriçao de DNA para receptores para TGF-b, activina A e proteína morfogênica óssea 7(BMP7 ou proteína osteogênica 1, OP-1). As células D407 expressam a presença de mRNA para membros da família SMAD de mensageiros intracelulares. O mensageiro SMAD2, mas nao SMAD1, deslocou-se do citoplasma para o núcleo sob o efeito de TGF-b1. CONCLUSAO As proteínas da superfamília TGF-b atuam sobre o EPR através de mensageiros intracelulares da família SMAD por estímulo de receptores específicos
