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1.
Intern Med ; 56(18): 2519-2522, 2017 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-28824075

RESUMEN

A 69-year-old woman was admitted to our hospital with the chief complaints of fever and fatigue. We initially treated the patient for a tick-borne disease after noticing a pustule on her leg; however, abdominal computed tomography (CT) showed multiple low-density areas in the liver and Chromobacterium violaceum was isolated from a blood culture. We diagnosed her with multiple liver abscesses secondary to Chromobacterium violaceum bacteremia. The patient was successfully treated with ciprofloxacin.


Asunto(s)
Bacteriemia/microbiología , Chromobacterium/aislamiento & purificación , Infecciones por Bacterias Gramnegativas/microbiología , Absceso Hepático/microbiología , Anciano , Antibacterianos/uso terapéutico , Bacteriemia/tratamiento farmacológico , Ciprofloxacina/uso terapéutico , Femenino , Humanos , Absceso Hepático/tratamiento farmacológico , Tomografía Computarizada por Rayos X
2.
Am J Physiol Cell Physiol ; 292(2): C740-8, 2007 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-16943246

RESUMEN

Sphingosine 1-phosphate (S1P) is a platelet-derived angiogenic lipid growth factor, modulating G-protein-coupled S1P(1) receptors (S1P(1)-R) to activate endothelial nitric oxide synthase (eNOS), as well as MAPK pathways in endothelial cells. We explored whether and how hydrogen peroxide (H(2)O(2)), a representative reactive oxygen species, alters S1P(1)-R expression and influences S1P signaling in cultured bovine aortic endothelial cells (BAECs). When BAECs are treated with pathophysiologically relevant concentrations of H(2)O(2) (150 microM for 30 min), S1P(1)-R protein expression levels are acutely augmented by approximately 30-fold in a dose-dependent fashion. When BAECs have been pretreated with H(2)O(2), subsequent S1P stimulation (100 nM) leads to a higher degree of eNOS enzyme activation (assessed as intracellular cGMP content, 1.7 +/- 0.2-fold vs. no H(2)O(2) pretreatment groups, P < 0.05), associated with a higher magnitude of phosphorylation responses of eNOS and MAPK ERK1/2. PP2, an inhibitor of Src-family tyrosine kinase, abolished the effects of H(2)O(2) on both S1P(1)-R protein upregulation and enhanced BAEC responses to S1P. H(2)O(2) does not augment S1P(1) mRNA expression, whereas VEGF under identical cultures leads to increases in S1P(1) mRNA signals. Whereas H(2)O(2) attenuates proliferation of BAECs, addition of S1P restores growth responses of these cells. These results demonstrate that extracellularly administered H(2)O(2) increases S1P(1)-R expression and promotes endothelial responses for subsequent S1P treatment. These results may identify potentially important points of cross-talk between reactive oxygen species and sphingolipid pathways in vascular responses.


Asunto(s)
Plaquetas/metabolismo , Células Endoteliales/fisiología , Endotelio Vascular/citología , Peróxido de Hidrógeno/farmacología , Lisofosfolípidos/fisiología , Óxido Nítrico Sintasa de Tipo III/metabolismo , Receptores de Lisoesfingolípidos/biosíntesis , Esfingosina/análogos & derivados , Animales , Bovinos , Células Cultivadas , Células Endoteliales/efectos de los fármacos , Activación Enzimática , Sistema de Señalización de MAP Quinasas/fisiología , Fosforilación , Transducción de Señal , Esfingosina/fisiología , Factor A de Crecimiento Endotelial Vascular/metabolismo
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