Invitro Activity of Four Commonly Available Antifungal Drugs against Dermatophytes Species Isolated From Clinical Samples Using Disk Diffusion Method in Mymensingh.

Dermatophytosis is an infection with fungi related to the skin, hair and/or nails. Fungal infections' treatment orally in dermatology has become a superior modality for the management of these conditions. In spite of increasing the number of antifungal drugs, treatment failure has been noticed against some cases as well as some relapse cases. To eradicate dermatophyte it is necessary to perform in vitro antifungal susceptibility testing of dermatophyte. Simple disk diffusion method in vitro assay may be used to assess antifungal propensity in dermatophytes. This cross sectional study was aimed to evaluate the antifungal activity of four antifungal drugs against several fresh clinical dermatophyte isolates by disk diffusion method at the out patient department with the collaboration of Microbiology department, Mymensingh Medical College, Mymensingh, Bangladesh from July 2013 to September 2014. Fifty three clinical dermatophytes species were isolated from patients suspected of having active dermatophytosis. Terbinafine, griseofulvin, fluconazole and itraconazole containing paper disks were used in disk diffusion method for evaluation in vitro activity of antifungal agents by measuring the mean diameter of inhibition around the disks. The isolates belong to three genera and three species as: Trichophyton Rubrum 44(83.04%), T. mentagrophytes 5(9.43%), Epidermophyton floccosum 4(7.55%). All isolates were significant to terbinafine. Terbinafine, itraconazole and griseofulvin were exposed in this study as the most ideal antifungal drugs for the treatment of dermatophytosis. For the detection of antifungal susceptibility of dermatophytes disk diffusion method is not only a simple but also a valuable method.

Detection of Human Papilloma virus by Molecular method from Patients Attending at Colposcopy Clinic of Mymensingh Medical College Hospital, Mymensingh.

Cervical cancer is the second most common cancer in women worldwide. Human papillomavirus (HPV) is considered as the main cause of invasive cervical cancer and cervical intra-epithelial neoplasia. High risk HPV DNA has been shown to be present in 99.7% of cervical cancers. So HPV DNA testing for screening of cervical cancers may play a potential role in early detection and management of cervical cancer. With above background a cross sectional study was undertaken to estimate the prevalence and to identify the associated risk factors of human Papillomavirus infection among Visual Inspection with Acetic acid (VIA) positive women attending at colposcopy clinic of Mymensingh Medical College Hospital (MMCH), Mymensingh, Bangladesh from July 2013 to December 2014. One hundred and forty three cervical swabs for nested PCR were collected from the patients attending colposcopy clinic of MMCH, for detecting target gene of L1 region of the HPV genome. Among the 143 VIA positive patient nested PCR showed 49.6% (71/143) positive. Biopsy of 54 colposcopy positive women revealed that 16 (29.6%) cases were chronic cervicitis, 33 (61.1%) cases were mild dysplasia (C1NI), 01 (1.9%) were having moderate dysplasia (C1NII) and 04(7.4%) patients were diagnosed as invasive squamous cell carcinoma. So, high grade cervical lesions were 100% positive by nested PCR for HPV.

Diagnosis of Dermatophytosis by Conventional Methods and Comparatative analysis of Sabouraud Dextrose Agar and Dermatophyte Test Medium for Isolation of Dermatophytes.

Dermatophytosis is a fungal disease which ranked as one of the most common cutaneous condition in all over the world. The aim of this cross sectional observational study is to identify the etiological agent of dermatophytosis and comparison of Sabouraud dextrose agar (SDA) and Dermatophyte test medium (DTM) for the primary isolation of dermatophytes among the clinically suspected cases of dermatophytosis from the outpatient Department of Dermatology and Venereology, Mymensingh Medical College Hospital from July 2013 to December 2015. Two hundred thirty clinically suspected cases of dermatophytosis were subjected in this study. Sixty three cases (27.4%) were positive for fungus in direct microscopy while 53(23%) were culture positive. Trichophyton rubrum (83%) was found common etiological dermatophyte species followed by Trichophyton mentagrophytes 5(9.4%) and Epidermophyton floccosum 4(7.5%). There was no statistically significant difference between the SDA and DTM (p<0.01) in primary isolation of dermatophytes.

Rotavirus is Predominent Enteropathogen in Acute Childhood Diarrhea in Mymensingh.

The study was carried out from July 2011 to June 2012 in the Department of Microbiology, Mymensingh Medical College. A total of 200 clinically diagnosed diarrheal paediatrics patients were included in this study. Stool specimens were examined by standard laboratory methods for identification of enteropathogens. Diarrheagenic Escherichia coli strains were detected by Multiplex PCR following standard methods and Rotavirus genome was detected by Polyacrylamide Gel Electrophoresis (PAGE). Rotavirus was the most common enteropathogen detected in 69(34.5%) cases, followed by Diarrheagenic Escherichia coli (DEC) and Campylobacter species in 34(17.0%) and 23(11.5%) cases respectively. Rotavirus detection rate was high in upto 1 year age group which was 51(25.5%).

Pattern of Dermatophytes in Patients Attend in Mymensingh Medical College Hospital.

Dermatophytosis is a common fungal disease which involves the keratinized tissue. This is an attempt to observe the spectrum of dermatophytes among the clinically suspected cases of dermatophytosis attending the outpatient department of Dermatology and Venereology, Mymensingh Medical College Hospital, Mymensingh during the period of July 2013 to December 2014. Two hundred thirty (230) clinically suspected cases of dermatophytosis were subjected in this study. Sixty three cases (27.39%) were positive for fungus in direct microscopy while 53(23.04%) were culture positive. Among the clinical types tinea unguium was the most common followed by tinea corporis. Dermatophytosis was more common in the age group of 21-30 years. The male to female ratio was 1.53:1. Trichophyton rubrum 44(83.04%) was found common etiological dermatophyte species followed by Trichophyton mentagrophytes 5(9.43%) and Epidermophyton floccosum 4(7.55%).