Error mitigation enables PET radiomic cancer characterization on quantum computers.

BACKGROUND: Cancer is a leading cause of death worldwide. While routine diagnosis of cancer is performed mainly with biopsy sampling, it is suboptimal to accurately characterize tumor heterogeneity. Positron emission tomography (PET)-driven radiomic research has demonstrated promising results when predicting clinical endpoints. This study aimed to investigate the added value of quantum machine learning both in simulator and in real quantum computers utilizing error mitigation techniques to predict clinical endpoints in various PET cancer patients. METHODS: Previously published PET radiomics datasets including 11C-MET PET glioma, 68GA-PSMA-11 PET prostate and lung 18F-FDG PET with 3-year survival, low-vs-high Gleason risk and 2-year survival as clinical endpoints respectively were utilized in this study. Redundancy reduction with 0.7, 0.8, and 0.9 Spearman rank thresholds (SRT), followed by selecting 8 and 16 features from all cohorts, was performed, resulting in 18 dataset variants. Quantum advantage was estimated by Geometric Difference (GDQ) score in each dataset variant. Five classic machine learning (CML) and their quantum versions (QML) were trained and tested in simulator environments across the dataset variants. Quantum circuit optimization and error mitigation were performed, followed by training and testing selected QML methods on the 21-qubit IonQ Aria quantum computer. Predictive performances were estimated by test balanced accuracy (BACC) values. RESULTS: On average, QML outperformed CML in simulator environments with 16-features (BACC 70% and 69%, respectively), while with 8-features, CML outperformed QML with + 1%. The highest average QML advantage was + 4%. The GDQ scores were ≤ 1.0 in all the 8-feature cases, while they were > 1.0 when QML outperformed CML in 9 out of 11 cases. The test BACC of selected QML methods and datasets in the IonQ device without error mitigation (EM) were 69.94% BACC, while EM increased test BACC to 75.66% (76.77% in noiseless simulators). CONCLUSIONS: We demonstrated that with error mitigation, quantum advantage can be achieved in real existing quantum computers when predicting clinical endpoints in clinically relevant PET cancer cohorts. Quantum advantage can already be achieved in simulator environments in these cohorts when relying on QML.

Efficacy of propolis and triple antibiotic paste as intra-canal medicaments for revascularisation of immature teeth in dogs: a comparative study.

AIM: Revascularisation is a therapeutic approach for treatment of immature teeth with necrotic pulp. The conventional protocol includes application of triple antibiotic paste (TAP). The present study aimed to compare the efficacy of propolis and TAP as intra-canal medicaments for revascularisation of immature teeth in dogs. METHODOLOGY: This study was conducted on 20 immature (open-apex) canine teeth in mixed breaded dogs. First, the teeth were exposed to oral environment, followed by intra-canal cleaning and shaping 2 weeks later.. The teeth were divided into two groups. The TAP group received paste comprising of ciprofloxacin, metronidazole, and minocycline (at a concentration of 100 µg/mL), while propolis (15% w/v) was used in the other group. The revascularisation procedure was done by means of sodium hypochlorite, EDTA and distilled water as final irrigant. After dehumidifying and induction of bleeding, mineral trioxide aggregate (MTA) was applied At 6-month follow-up, radiographs were evaluated by two observers regarding root length and thickness, intra-canal calcification, periapical lesions, and apex formation. Data were analysed by the Chi-square and Fisher's exact tests. RESULTS: There was no significant difference between the TAP and propolis groups in root length or root thickness increase, calcification, related lesions, or apex formation (P > 0.05). CONCLUSION: Findings of the present study in experimental animals revealed that the efficacy of propolis as an intra-canal medicament is comparable to that of triple antibiotic paste for revascularisation therapy.

The Association between Dietary Inflammatory Index and Aging Biomarkers/Conditions: A Systematic Review and Dose-response Meta-analysis.

OBJECTIVES: We performed a current study to examine the association between dietary inflammatory index (DII) score and older age-related muscle conditions, including sarcopenia, low muscle mass, low muscle strength, frailty, and/or disability. DESIGN: Systematic review and dose-response meta-analysis. SETTING: A systematic literature search was performed using Scopus, PubMed/MEDLINE, and ISI Web of Science without limitation until October 04, 2022. Relative risk (RR) and 95% confidence interval (CI) were pooled by applying a random-effects model, while validated methods examined assess quality and publication bias via Newcastle-Ottawa Scale, Egger's regression asymmetry, and Begg's rank correlation tests respectively. A dose-response meta-analysis was conducted to estimate the RRs per 1-unit increment in DII scores. PARTICIPANTS: Adults (≥18 years). MEASURES: The risk of older age-related muscle conditions (sarcopenia, low muscle mass, low muscle strength, frailty, and/or disability). RESULTS: Data were available from 19 studies with 68079 participants. Results revealed that a higher DII score was significantly related to an increased risk of sarcopenia (RR=1.50; 95% CI: 1.26, 1.79; I2=53.3%; p<0.001; n=10; sample size =43097), low muscle strength (RR=1.47; 95% CI: 1.24, 1.74; I2=6.6%; p<0.001; n=4; sample size =9339), frailty (RR=1.61; 95% CI: 1.41, 1.84; I2=0.0%; p<0.001; study=5; participant=3882) and disability (RR=1.41; 95% CI: 1.16, 1.72; I2=58.4%; p=0.001; n=5; sample size =13760), but not low muscle mass (RR=1.24; 95% CI: 0.98, 1.56; I2=49.3%; p=0.069; n=4; sample size =11222). Additionally, results of the linear dose-response indicated that an increase of one point in the DII score was related to a 14% higher risk of sarcopenia, 6% higher risk of low muscle mass, 7% higher risk of low muscle strength, and a 7% higher risk of disability in adults. Non-linear dose-response relationships also revealed a positive linear association between the DII score and the risk of sarcopenia (Pnonlinearity = 0.097, Pdose-response<0.001), frailty (Pnonlinearity = 0.844, Pdose-response=0.010) and disability (Pnonlinearity = 0.596, Pdose-response=0.007). CONCLUSION: Adherence to a pro-inflammatory diet was significantly associated with a higher risk of sarcopenia and other age-associated adverse effects such as low muscle strength, disability, and frailty. These results indicate a necessity to prioritize the reduction of pro-inflammatory diets to help promote overall older age-related muscle conditions.

Monensin supplementation downregulated the expression signature of genes involved in cholesterol synthesis in the ruminal epithelium and adipose tissue of lambs.

To understand the metabolic mechanisms regulating lipid metabolism by monensin, Afshari male lambs (n = 16) with 41.0 ± 2.4 kg body weight (BW, mean ± SD) at approximately 180 days of age were randomly assigned equally to two dietary treatments. After a 21-day pre-adaptation period, all animals in two groups continued to receive the basal diet, but one group received no monensin supplementation (control) while the other group received 30 mg/day of monensin per animal. Individual BW was recorded weekly to determine the average daily body weight gain (ADG). At the end of the 56-day experimental period, lambs were weighed and slaughtered. Monensin supplementation did not affect BW, ADG, and rumen fermentation characteristics. However, monensin significantly downregulated the sterol regulatory element-binding protein (SREBP)-2 gene expression in all sample tissues (p < 0.05). Also, monensin downregulated expressions of SREBP-1c and peroxisome proliferator-activated receptor (PPAR)-γ in back fat tissues. Monensin increased the expression of 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS)-2, but it decreased the mRNA abundance of HMGCS-1 in the rumen epithelial tissues (p < 0.05). Our data suggest that monensin downregulates cholesterol synthesis via inhibition of HMGCS-1 and impairment of the SREBP pathway, probably due to a crosstalk among different tissues to control energy metabolism.

[From surgery for esophageal atresia in the infancy to late complications of ib fusion and scoliosis]. / L'image du mois. D'une chirurgie pour atrésie de l'oesophage dans l'enfance à des complications tardives de type fusion costale et scoliose.

Following surgey for esophageal atresia, orthopedic complications can sometimes appear late in childhood, hence the importance of regular monitoring. These complications include rib fusion secondary to thoracic surgery and possible infectious complications. Undiagnosed, this condition can lead to severe scoliosis in adolescence.

Après une chirurgie thoracique pour une atrésie de l'œsophage, les complications orthopédiques peuvent apparaître parfois tardivement durant l'enfance, d'où l'importance d'un suivi régulier. Parmi ces complications figure la fusion costale secondaire à l'intervention chirurgicale et aux éventuelles complications infectieuses. Non diagnostiquée et traitée, cette complication peut être responsable d'une scoliose sévère à l'adolescence.

Clinical data classification with noisy intermediate scale quantum computers.

Quantum machine learning has experienced significant progress in both software and hardware development in the recent years and has emerged as an applicable area of near-term quantum computers. In this work, we investigate the feasibility of utilizing quantum machine learning (QML) on real clinical datasets. We propose two QML algorithms for data classification on IBM quantum hardware: a quantum distance classifier (qDS) and a simplified quantum-kernel support vector machine (sqKSVM). We utilize these different methods using the linear time quantum data encoding technique ([Formula: see text]) for embedding classical data into quantum states and estimating the inner product on the 15-qubit IBMQ Melbourne quantum computer. We match the predictive performance of our QML approaches with prior QML methods and with their classical counterpart algorithms for three open-access clinical datasets. Our results imply that the qDS in small sample and feature count datasets outperforms kernel-based methods. In contrast, quantum kernel approaches outperform qDS in high sample and feature count datasets. We demonstrate that the [Formula: see text] encoding increases predictive performance with up to + 2% area under the receiver operator characteristics curve across all quantum machine learning approaches, thus, making it ideal for machine learning tasks executed in Noisy Intermediate Scale Quantum computers.

[Knee pain in a child : watch out for the hip !] / Gonalgie chez l'enfant : vérifier la hanche !

This is a case report of a 13-year old patient presenting with knee pain after a bike accident. Multiple investigations and medical examinations focused on the knee. They did not show any abnormality. About one year later, the patient comes back with increased hip pain, limited internal rotation and shortening of the left limb. Hip X-Ray exams reveal a bilateral slipped capital femoral epiphysis requiring already an osteotomy. Slipped capital femoral epiphysis is a disorder of the proximal femoral epiphysis. The femoral neck is displaced anteriorly and rotates externally while the epiphysis remains in the acetabulum. This pathology is more common in preadolescent boys and can be associated with hormonal disorders. Patients present with an externally rotated gait, a limited internal rotation, associated hip and knee pain. Diagnosis might be missed when patients present with knee pain and can lead to delayed treatment. When facing knee pain, it is essential to perform a clinical examination of the hip. Radiography is the first medical imaging. Surgical treatment by in situ fixation is recommended and prevents further slip with a high rate of success. When delayed, a much more invasive approach is required. A delay in diagnosis adversely affects long-term outcomes.

Il s'agit d'un patient de 13 ans présentant des gonalgies comme principale plainte. Vu l'apparition des douleurs suite à une chute, les investigations se sont focalisées sur le genou. De multiples examens complémentaires ont été réalisés sans mise en évidence de lésion. Après un an d'évolution, le patient se présente en consultation d'orthopédie en chaise roulante avec une impotence fonctionnelle majeure et des douleurs importantes au niveau des membres inférieurs. On note une boiterie, une inégalité des membres inférieurs et une raideur des hanches. Une radiographie du bassin démontre une épiphysiolyse fémorale supérieure bilatérale. Il s'agit d'un glissement de l'épiphyse par rapport au col fémoral, retrouvé plus fréquemment chez le garçon entre 10 et 15 ans. Le tableau clinique peut être caractérisé par des gonalgies, entrainant des retards diagnostiques. En cas de diagnostic précoce, un vissage in situ, peu invasif, empêche la progression de la déformation. Si le diagnostic est retardé, un traitement plus lourd et plus invasif comportant plus de risques et de moins bons résultats à long terme doit être pratiqué. En cas de mauvaise évolution, une arthroplastie précoce doit être réalisée. Une prise en charge rapide permet d'éviter cette escalade thérapeutique.

Teaching Minimally Invasive Interventions in Paediatric Dentistry: A Cross-Sectional Survey of Dental Schools in Iran.

BACKGROUND: Dental caries is a significant public health problem in Iran. Teaching  minimally invasive interventions in paediatric dentistry may facilitate the provision of treatment for untreated dental caries in children. We evaluated the teaching of such interventions in both undergraduate dental curriculum and Paediatric Dentistry Specialty Training Programme (PDSTP) in Iran. METHODS: This was a cross-sectional questionnaire-based survey. Participants in this study were the directors of 40 undergraduate programmes and 15 PDSTPs in all Iranian dental schools (response rate = 100%). Descriptive statistics were reported. RESULTS: The most commonly taught methods were preventive fissure sealant and preventive resin restoration (PRR), which were taught 'both didactically and clinically' in all undergraduate dental programmes. The least commonly taught methods were silver diamine fluoride (SDF), the Hall technique and resin infiltration, which were taught 'both didactically and clinically' in less than 5% of dental schools. The same three methods were the least commonly approaches taught in PDSTP, further, they were less often perceived to be 'essential'. CONCLUSIONS: There was a notable variation in the teaching of the management of dental caries in Iran's dental education. Some minimally invasive approaches including SDF, the Hall technique and resin infiltration are not being commonly taught in Iranian dental schools despite the evidence base for these techniques.

Exposure to heavy metals and the risk of osteopenia or osteoporosis: a systematic review and meta-analysis.

The relationship between heavy metal exposure and risk of osteopenia or osteoporosis has biological plausibility, yet it remains inconclusive; therefore, we conducted a systematic review and meta-analysis to evaluate the associations between exposure to heavy metals (i.e., cadmium, lead, and mercury) and the risk of osteopenia or osteoporosis. Databases of MEDLINE, Embase, Scopus, and Web of Science were searched through November 2019, to identify studies that evaluated the relationship between exposure to cadmium, lead, and mercury and risk of osteopenia or osteoporosis in adults. Fourteen eligible studies were included. Effect sizes expressed as pooled odds ratios (OR) and 95% confidence intervals (CI) were estimated using weighted random-effect models. Exposure to cadmium (OR = 1.35; 95% CI: 1.17 to 1.56; P ≤ 0.001) and lead (OR = 1.15; 95% CI: 1.00 to 1.32; P = 0.05) was associated with an increased risk of osteopenia or osteoporosis, unlike mercury. Subgroup analyses showed cadmium exposure increased the risk of osteopenia or osteoporosis in older (> 65 yrs.; OR = 1.43; 95%CI: 1.08 to 1.88, P = 0.01) compared with younger (18-65 yrs.; OR = 1.24; 95% CI: 1.02 to 1.52, P = 0.03) adults. Also, lead exposure increased the risk in men (OR = 1.55; 95% CI: 1.15 to 2.09, P = 0.007) unlike in women. By contrast to urinary levels, blood (OR = 1.26; 95% CI: 1.08 to 1.47, P = 0.003) and dietary (OR = 1.46; 95% CI: 1.28 to 1.67, P < 0.001) levels of cadmium were associated with an increased risk of osteopenia or osteoporosis. Exposure to cadmium and lead may be associated with an increased risk of osteopenia or osteoporosis, although high heterogeneity was detected.

Greater child dental health inequality in England compared to Wales and Northern Ireland, despite lower average disease levels.

INTRODUCTION: Dental caries and inequalities in dental health are major public health concerns. AIM: To report variation in dental caries experience across deprivation quintiles and the magnitude of inequalities between countries. DESIGN: Secondary analyses of cross-sectional data from the 2013 Child Dental Health Survey (CDHS) in England, Wales, and Northern Ireland. MATERIALS AND METHODS: Distribution of dental caries across deprivation quintiles were estimated using as proportions and means. The magnitude of inequalities was calculated using the Relative Index of Inequality (RII). MAIN OUTCOMES: Dental caries experience as indicated by the prevalence (%dmft/DMFT>0) and severity (dmft/DMFT) of 'obvious' and 'clinical' decay experience in both primary and permanent dentitions. RESULTS: Children from more deprived quintiles showed higher prevalence and severity of dental caries. RIIs for dental caries were greater in England than Wales or Northern Ireland, indicating greater relative inequalities despite lower average dental caries experience. The prevalence and severity of dental caries among the most deprived children in England were 1.7 to 3.7 times greater than those of the least deprived. CONCLUSION: There is a deprivation gradient in child dental caries in all three countries, with England showing the greatest inequalities.

Role of components of microRNA machinery in carcinogenesis.

MicroRNAs (miRNAs) are a broad class of non-coding RNAs nearly 21 nucleotides length, which play crucial functions in post-transcriptional gene regulation. These molecules are associated with many developmental and cellular processes in eukaryotic organisms. Current investigation has reported major factors contributing to miRNA biogenesis and has constituted basic principles of miRNA function. More recently, it was confirmed that various miRNAs are clearly implicated in human malignancies, such as lung, breast, ovarian, bladder, colon cancer and other kinds of carcinoma. In addition, dysregulation in the miRNA machinery elements such as Dicer, Drosha, DGCR8, Argonaut, and TRBP could be involved in the progress of many tumor types. The purpose of the current review was to compile growing information besides how miRNA biogenesis and gene silencing are modified to develop cancer.

Association between serum 25(OH) vitamin D and metabolic disturbances in polycystic ovary syndrome.

BACKGROUND: To assess the relationship between serum 25(OH) vitamin D levels and metabolic parameters together with androgenic hormone levels in women with polycystic ovary syndrome (PCOS). METHODS: This is a single-centre study from the Endocrinology Institute at Firouzgar Hospital in Iran. Seventy-three women aged 15-45 years were recruited from May 2013 to December 2013. Serum 25(OH) vitamin D level, other laboratory biomarkers and anthropometric indexes were measured. Data were analysed with statistical software SPSS version 16.0 for windows and we used specific analytical tests to assess data. RESULTS: Serum 25(OH) vitamin D levels were < 20 ng/ml in 64 patients (79%). Nine patients (12.3%) were diagnosed with metabolic syndrome. A significant difference was found between the women with and without severe vitamin D deficiency with regard to waist circumference, fasting insulin level and HOMA-IR, and abnormal systolic and diastolic blood pressures. The serum 25(OH) vitamin D levels had a reverse but weak correlation with systolic and diastolic blood pressures. CONCLUSION: This study showed an association between serum 25(OH) vitamin D levels and some metabolic parameters; however, there was no significant linear correlation between serum 25(OH) vitamin D levels and metabolic variables, except for systolic and diastolic blood pressure.

Adipokines may mediate the relationship between resting metabolic rates and bone mineral densities in obese women.

The researchers sought to test the possible link between resting metabolic rate and bone mineral density through four adipokines. Participants with lower resting metabolic rate (RMR) per kilogram demonstrated higher total bone mineral density (BMD), total T-score, and total Z-score. Omentin-1 had a mediatory effect on the relationship between RMR/kg of body weight and bone parameters. INTRODUCTION: The previous results of studies regarding the links between obesity and bone health are controversial. For this reason, the researchers sought to test the possible link between RMR and BMD through the following four adipokines: vaspin, retinol binding protein 4, angiopoietin-like 6 (ANGPL6), and omentin-1. METHODS: We enrolled 312 obese Iranian women (30 ≤ body mass index <40) in this cross-sectional study. In order to examine the association of serum adipokine levels with RMR and BMD, the participants were grouped based on RMR per body weight. Body composition, dietary intake, bone mineral density, and resting metabolic rate were assessed in all participants. Serum adipokine levels were quantified by the enzyme-linked immunosorbent assay (ELISA) method. RESULTS: Low levels of RMR/kg were strongly associated with higher weight, body mass index, fat mass, and visceral fat levels. In fact, participants with an RMR/kg of body weight <20 kcal/24 h/kg were more obese (p < 0.05). Another noteworthy finding was that participants with lower RMR/kg demonstrated higher total BMD, total T-score, and total Z-score. Our results showed that omentin-1 had a mediatory effect on the relationship between RMR per kilogram of body weight and bone parameters (p < 0.05). Nevertheless, other adipokines such as vaspin, retinol-binding protein 4 (RBP4), and ANGPL6 did not affect the relationship between RMR and BMD (p > 0.05). CONCLUSIONS: The inhibitory effect of omentin-1 on TNF-alpha seems to be able to reduce the amount of circulating leptin as adipokine, affecting energy expenditure and improving bone loss induced by estrogen deficiency and controlled effect of RMR on BMD.

Is Stress Hyperglycemia a Predicting Factor of Developing Diabetes in Future?

OBJECTIVE: Stress hyperglycemia is a transient increase in blood glucose during an acute physiological stress in the absence of diabetes. Stress hyperglycemia can be occurred in certain clinical conditions such as trauma, burns, sepsis and strokes in adults. In this study we aimed to evaluate the incidence of stress hyperglycemia among patients admitted to the Firouzgar hospital. METHOD: In this analytical cross sectional study, all patients who referred to emergency department of Firouzgar hospital due to one of the causes of trauma, myocardial infarction (MI), subarachnoid hemorrhage (SAH), head trauma, sepsis, CVA, and abdominal surgery and had stress hyperglycemia during 2012-2014 were evaluated. Blood glucose test including random blood sugar (BS) and glycosylated hemoglobin (HbA1C) was performed for patients at admission. Patients with BS>180 mgmg/dl and no previous history of diabetes were enrolled in this study. Patients were referred to endocrinology clinic after 3 months of the first test and were re-evaluated for diabetes mellitus. Fasting Blood Sugar (FBS), HbA1C and 2 h post glucose load test blood sugar were measured. Diabetes was confirmed if FBS was above 126 mg/dl or 2 h post glucose load BS was over 200 mg/dl. RESULT: A total of 98 (67 males & 31 women) patients enrolled in the study. No significant statistical relation found between mean of HBA1C at 3 months after admission and the background event (P=0.138). No statistical relation found between BMI and developing diabetes (P=0.352). 26 and 8 percent of the participants developed diabetes, and 25.8% were in pre-diabetic state. There was a statistical relation between gender and developing diabetes (P=0.027) and men developed diabetes more than women. Based on logistic regression the Odds Ratio (OR) was 1.017. CONCLUSION: Our study demonstrates a significant relationship between stress hyperglycemia and development of diabetes. Stress hyperglycemia could be a predicting factor of development of diabetes.

Differential expression of RFamide-related peptide, a mammalian gonadotrophin-inhibitory hormone orthologue, and kisspeptin in the hypothalamus of Abadeh ecotype does during breeding and anoestrous seasons.

Gonadotrophin-inhibitory hormone (GnIH) is a novel hypothalamic neuropeptide that was discovered in birds as an inhibitory factor for gonadotrophin release. RFamide-related peptide (RFRP) is a mammalian GnIH orthologue that inhibits gonadotrophin synthesis and release in mammals through actions on gonadotrophin-releasing hormone (GnRH) neurones and gonadotrophs, mediated via the GnIH receptor (GnIH-R), GPR147. On the other hand, hypothalamic kisspeptin provokes the release of GnRH from the hypothalamus. The present study aimed to compare the expression of RFRP in the dorsomedial hypothalamus and paraventricular nucleus (DMH/PVN) and that of kisspeptin in the arcuate nucleus (ARC) of the female goat hypothalamus during anoestrous and breeding seasons. Mature female Abadeh does were used during anoestrus, as well as the follicular and luteal phases of the cycle. The number of RFRP-immunoreactive (-IR) neurones in the follicular phase was lower than in the luteal and anoestrous stages. Irrespective of the ovarian stage, the number of RFRP-IR neurones in the rostral and middle regions of the DMH/PVN was higher than in the caudal region. By contrast, the number of kisspeptin-IR neurones in the follicular stage was greater than in the luteal stage and during the anoestrous stage. Irrespective of the stage of the ovarian cycle, the number of kisspeptin-IR neurones in the caudal region of the ARC was greater than in the middle and rostral regions. In conclusion, RFRP-IR cells were more abundant in the rostral region of the DMH/PVN nuclei of the hypothalamus, with a greater number being found during the luteal and anoestrous stages compared to the follicular stage. On the other hand, kisspeptin-IR neurones were more abundant in the caudal part of the ARC, with a greater number recorded in the follicular stage compared to the luteal and anoestrous stages.

Master file size and apical transportation in severely curved root canals.

OBJECTIVES: A curvature leads to asymmetrical removal of root dentine, which results in an increase in the incidence of canal transportation. The aim of this study was to evaluate the effect of master apical file (MAF) size on the apical transportation (AT) of teeth with severe canal curvatures. METHODS: Thirty-six mesial roots of human mandibular first molars were included. Inclusion criteria consisted of mature roots with closed apices, absence of carious lesions and resorptions, and root canal curvatures (CC) of 45 degrees < CC < 60 degrees. The root canals were prepared using a crown-down pressureless technique. The samples were equally divided into groups A, B, and C based on MAF size: group A: MAF size equivalent to #20; group B: MAF size equivalent to #25, and group C: MAF size equivalent to #30. Cone beam computed tomography was used to evaluate the AT. RESULTS: There were no statistically significant differences in the AT between the canals with different curvature angles up to MAF size #30 (P = 0.55). CONCLUSION: Using flexible hand files for canal preparation, an increase in MAF size up to #30 does not significantly influence AT in severely curved canals.

The effects of aqueous extract of Boswellia Serrata on hippocampal region CA1 and learning deficit in kindled rats.

Temporal lobe epilepsy (TLE) is a disorder of the central nervous system in which hippocampus is mostly involved and causes memory impairment. Kindling is a model of inducing epilepsy which is created through pentylenetetrazol (PTZ) administration. This study examines the role of the aqueous extract of Boswellia on the learning and development of brain (formation of dendritic branches and axons) of the PTZ-induced kindled rats. The study is conducted on sixty-four male rats divided into 8 groups. Kindling seizures are induced by three injections of 25 mg/kg of PTZ every 15 min. The aqueous extracts (0, 0.1, 0.5, 1 g/kg, i.p) are administrated to all animals for three consecutive days. Passive avoidance learning of animals is examined using shuttle box apparatus and step-through latency (STL) method. Rats are anesthetized and their brains are fixed by transcardial perfusion method and are analyzed by morphometric methods after applying Golgi and Cresyl violet staining methods. PTZ-induced kindling indicates a significant decrease in the number of pyramidal neurons and dendritic spines in hippocampal region cornu ammonis (CA1). The STL of the kindled rats is significantly reduced compared with control ones. Also, Boswellia extract dramatically increased the number of neuronal processes in CA1 region and improves passive-avoidance learning ability in both control and PTZ-kindled animals in 1 g/kg dose administration of Boswellia extract, especially at high doses can eliminate adverse effects of seizures on cognitive function in hippocampal area CA1 in rats.

The effect of increasing feeding frequency on performance, plasma hormones and metabolites, and hepatic lipid metabolism of broiler breeder hens.

An experiment was conducted to study the effects of feeding regimens on reproductive performance, plasma hormone and metabolite levels, and hepatic lipid metabolism of Cobb 500 broiler breeder hens from 26 to 38 wk of age. Seventy-two birds were used in a completely randomized design with 3 treatments, each replicated 4 times. Treatments were as follows: 1) once a day feeding, in which birds were fed once a day at 0615 h (control), 2) twice a day feeding, in which daily allocated feed was fed in 2 equal meals at 0615 and 1215 h, and 3) thrice a day feeding in which daily allocated feed was offered in 3 equal meals at 0615, 1215, and 1815 h. Through 38 wk of age, total hen-day egg production in the hens fed twice and thrice a day was greater (67.1 and 67.2 vs. 62.2 eggs/hen, P < 0.01). Similarly, egg weight was higher (P < 0.01) in birds fed more than once a day. Multi-meal-fed birds had significantly lower plasma triiodothyronine and glucose at 32 wk and also lower glucose and cholesterol, and higher 17ß-estradiol levels at 38 wk than those fed once a day (P ≤ 0.05). Hepatic expression of malic enzyme, fatty acid synthase, acetyl-CoA carboxylase, and ATP citrate lyase relative to ß-actin decreased (P < 0.05) in birds fed twice and thrice a day compared with birds fed once a day at peak egg production (32 wk). In contrast, feeding regimens did not affect the hepatic gene expression of lipogenic enzymes after peak egg production at 38 wk. Stearoyl-CoA desaturase 1 (SCD1) gene expression was constant over dietary regimens. There was no difference in malic enzyme activity in multi-meal-fed birds at 38 wk. In summary, feeding broiler breeder hens 2 or 3 meals per day improved the reproductive performance during the early lay cycle. Implementing twice or thrice a day feeding regimens altered hepatic lipogenic gene expression in broiler breeder hens only at peak egg production, which indicated a short-term effect of increasing feeding frequency on hepatic lipid metabolism.

Trends in cardiovascular disease risk factors in children and adolescents: Tehran lipid and glucose study.

Studies have shown that cardiovascular risk factors, such as obesity, blood lipid levels, start early in childhood and some are on the rise in children. Data on trends are important in order to identify if there is a problem. This study, part of the Tehran Lipid and Glucose Study, determined and compared the prevalence of overweight and its associated risk factors in 2555, 1 329 and 1158 Tehran children and adolescents in 2000, 2003 and 2006 respectively. The participants were categorized into age groups 3-6, 7-12 and 13-17 years. Body mass index measurements were taken and blood pressure, fasting blood glucose, cholesterol and triglycerides measured. Overall the prevalence of obesity in Tehran children and adolescents increased significantly from 2000 to 2006 while blood pressure and serum lipid concentrations decreased. The causes for the decreased blood pressure and serum lipid concentrations should be evaluated. The increased prevalence of obesity in Tehran children and adolescents is of concern and requires monitoring.

Trends in cardiovascular disease risk factors in children and adolescents: Tehran Lipid and Glucose Study

Studies have shown that cardiovascular risk factors, such as obesity, blood lipid levels, start early in childhood and some are on the rise in children. Data on trends are important in order to identify if there is a problem. This study, part of the Tehran Lipid and Glucose Study, determined and compared the prevalence of overweight and its associated risk factors in 2 555, 1 329 and 1 158 Tehran children and adolescents in 2000, 2003 and 2006 respectively. The participants were categorized into age groups 3-6, 7-12 and 13-17 years. Body mass index measurements were taken and blood pressure, fasting blood glucose, cholesterol and triglycerides measured. Overall the prevalence of obesity in Tehran children and adolescents increased significantly from 2000 to 2006 while blood pressure and serum lipid concentrations decreased. The causes for the decreased blood pressure and serum lipid concentrations should be evaluated. The increased prevalence of obesity in Tehran children and adolescents is of concern and requires monitoring