Retrospective Evaluation of Clinical Signs and Gross Pathologic Findings in Birds Infected With Mycobacterium genavense.

Mycobacterium genavense is regarded as the primary cause of mycobacteriosis in passerine and psittacine birds kept in captivity. Mycobacterium genavense is a potential zoonotic pathogen; therefore, early antemortem detection in birds is needed. In humans, infections with M genavense are found predominantly in immunocompromised people. To investigate clinical signs and pathologic lesions and to determine the prevalence of coinfections in birds infected with M genavense, we reviewed records of 83 birds in which DNA from M genavense had been detected via real-time polymerase chain reaction. To evaluate clinical signs in birds presented as patients, results of standardized examinations of 60 birds and radiographic results from 37 birds were investigated. Necropsy results of 82 of the 83 birds were evaluated, including results of additional parasitologic, bacteriologic, and virologic examinations. Birds included in the study comprised 15 species in the orders Passeriformes, Psittaciformes, Coliiformes, Columbiformes, Coraciiformes, and Ciconiiformes. A wide range of clinical manifestations were documented, including neurologic disorders, ocular manifestations, and gastrointestinal signs. Of the 60 birds examined clinically, 15% showed no clinical signs. Coinfections with a wide range of pathogens were detected in 52% (43 of 83) of the tested birds. Coinfections included Macrorhabdus ornithogaster, circovirus, polyomavirus, avian bornavirus, adenovirus, Mycobacterium avium ssp. avium/ silvaticum, Mycoplasma species, Salmonella species, Escherichia coli, Aspergillus species, and various parasites. The high number of coinfections may reflect an impaired immune status in the birds examined. These results also suggest a broad host range for M genavense, and the existence of various clinical signs that may be strongly associated with coinfections with other pathogens.

[Three-dimensional ultrasonographic examination of the avian eye: technique and physiological findings]. / Die dreidimensionale sonographische Untersuchung des Vogelauges.

OBJECTIVE: The paper describes the possibilities and the clinical utility of three-dimensional (3D) ultrasonography in the avian eye. MATERIAL AND METHODS: The healthy eyes of 44 patients (six various raptor, three psittacine bird and four other bird species) were examined using the Voluson i ultrasound unit (GE Healthcare, Austria) in combination with a high-resolution 18 MHz linear probe. Physiological findings should be demonstrated to obtain a clinical basis for the evaluation of pathological ocular findings. Additionally, the blood flow of the pecten oculi was assessed using 3D colour Doppler ultrasonography. RESULTS: By means of 3D ultrasonography, the physiological structures in the avian eye could be demonstrated in their spatial context for the first time. In addition, the 3D appearance of the blood flow of the pecten oculi was shown. CONCLUSION AND CLINICAL RELEVANCE: 3D ultrasonography is a valuable diagnostic addition to classical optical-based ophthalmological examination of the avian eye and becomes essential when the posterior segment of the eye is obscured, as, for example, in opacities of the anterior dioptric apparatus or in patients with haemorrhage in the anterior eye chamber. The method may significantly enhance ophthalmological diagnostics in birds, particularly in situations with frequently occurring posttrauma intraocular haemorrhage as well as retinal detachment or lesions of the pecten oculi.

Identification and genetic characterization of polyomaviruses in estrildid and fringillid finches.

Polyomavirus infections were detected in 40 companion bird individuals belonging to a broad species range of estrildid and fringillid finches and originating from 21 different bird aviaries. Based on partial virus protein 1 (VP1) sequences, the viruses were identified as Serinus canaria polyomavirus 1 and Pyrrhula pyrrhula polyomavirus 1. Serinus canaria polyomavirus 1 was found in 18 birds belonging to one estrildid and four fringillid species. Pyrrhula pyrrhula polyomavirus 1 was detected in 22 birds of six estrildid and three fringillid species. There was a large overlap in host range. Increased mortality was frequently found in the affected bird aviaries while clinical signs were diverse. Co-infections with other viruses, bacteria or fungal pathogens were common and might have influenced the clinical signs. Sequence analyses, including partial VP1 sequences of the 40 virus strains, and full genome sequences of selected strains revealed a high genetic heterogeneity among virus subgroups of Serinus canaria polyomavirus 1 and Pyrrhula pyrrhula polyomavirus 1, indicating the existence of two virus variants for both virus species. For Pyrrhula pyrrhula polyomavirus 1, two genotypes were found that associated with the family of the finches, Estrildidae or Fringillidae.