P27-A149†Liverpool research eye biobank-our exerience of expanding to collect from living donors.

PURPOSE: The Liverpool Research Eye Biobank (LREB) collects tissue for researchers who wish to study a wide range of ophthalmic conditions and develop new and more effective treatments. Historically the LREB has collected whole globes and conjunctiva from cadaveric donors but in 2021 we expanded to start collecting tissues from living donors who were undergoing ophthalmic surgery in the St Paul's Eye Unit in Liverpool. The aim was to provide tissue and fluid samples from patients with specific eye disease to research projects and create a bank of ophthalmic samples that can be provided to future research projects. Here we reflect on our experience after a year of collections. METHODS: The clinical team discuss donation with patients during the pre-op appointment. Consent is taken on the day of surgery using an electronic consent form available on PENS. Samples are taken according to the patient's consent preference and then stored appropriately within a fridge/freezer close to theatre. Samples are then transferred for processing to the University of Liverpool (UoL). Fluids such as aqueous and vitreous are preserved at -80°C. The majority of ocular tissue collected is preserved by fixing in 10% neutral buffered formalin then transferred to 70% ethanol for long term storage. On request samples have been preserved using alternative methods such as snap freezing in liquid nitrogen. All samples are logged using a laboratory information management system. RESULTS: Collections depend on the cooperation of the clinical teams and we have had very good engagement from them. The UoL works closely with St Pauls Eye Unit and the physical proximity between the two has been helpful. The location of the storage fridges close to theatre is important to limit extra effort for busy clinical teams. Regular training of consenters was key to ensure compliance with SOPs. In 11 months, we consented 419 donors and collected 673 samples including corneal tissue, iris, sclera, lens/capsule, retinal membranes, tenons, muscle, aqueous, vitreous, blood. CONCLUSION: After the success of collections from one site we plan to expand to collect from multiple sites including Aintree and Alder Hey Children's Hospital.

34†Decreased opportunities to approach next of kin for eye donation to the liverpool research eye bank due to COVID-19.

BACKGROUND: The Liverpool Research Eye Bank (LREB) specialises in collection and storage of ocular tissues for use in projects investigating ophthalmic diseases and potential treatments. In collaboration with the Liverpool Eye Donation Centre (LEDC), we collect whole eyes from cadaveric donors. The LEDC screens potential donors and approaches next-of-kin for consent on behalf of the LREB; however, there are factors which reduce the pool of donors such as transplant suitability, time constraints, medical contraindications and other complications. During the past 21 months, COVID-19 has been a big contraindication to donation. The study aimed to determine how much of an impact COVID-19 has had on donations to the LREB. METHODS: Between January 2020 and October 2021, the LEDC compiled a database detailing the results of decedent screen at The Royal Liverpool University Hospital Trust site. From these data the suitability of each decedent for transplant, research or unsuitable for both was extrapolated along with the number of decedents unsuitable specifically due to having COVID-19 at the time of death. Data on the number of families then approached regarding donation for research, the number who gave consent and the number of tissues collected were also included. RESULTS: The LREB did not collect any tissues from decedents who had COVID-19 listed on their death certificate during 2020 and 2021. The number of unsuitable donors for transplant or research increased considerably due to COVID-19 positivity, in particular, during the months of Oct 2020-Feb 2021. This led to decreased approaches being made to next of kin. Interestingly, COVID-19 did not appear to have directly affected the number of donations. The number of donors consented ranged from 0-4 per month throughout the 21 months, with no correlation to the months when COVID-19 deaths were at their highest. CONCLUSIONS: The lack of an association between COVID-19 cases on donor numbers suggests that the donation rates are influenced by other factors. Increasing awareness of the opportunity for donation for research may increase donation rates. Development of informational materials and organisation of outreach events will aid in this goal.