RESUMEN
Analysis of convalescent plasma derived from individuals has shown that IgG3 has the most important role in binding to SARS-CoV-2 antigens; however, this has not yet been confirmed in large studies, and the link between binding and neutralization has not been confirmed. By analyzing plasma pools consisting of 247-567 individual convalescent donors, we demonstrated the binding of IgG3 and IgM to Spike-1 protein and the receptor-binding domain correlates strongly with viral neutralization in vitro. Furthermore, despite accounting for only approximately 12% of total immunoglobulin mass, collectively IgG3 and IgM account for approximately 80% of the total neutralization. This may have important implications for the development of potent therapies for COVID-19, as it indicates that hyperimmune globulins or convalescent plasma donations with high IgG3 concentrations may be a highly efficacious therapy.
Asunto(s)
Anticuerpos Neutralizantes/inmunología , Anticuerpos Antivirales/inmunología , COVID-19/sangre , COVID-19/inmunología , Convalecencia , Inmunoglobulina G/inmunología , Animales , Anticuerpos Neutralizantes/sangre , Anticuerpos Antivirales/sangre , Chlorocebus aethiops , Ensayo de Inmunoadsorción Enzimática , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Inmunoglobulina M/inmunología , SARS-CoV-2/fisiología , Células VeroRESUMEN
Fluorescent probes that change their spectral properties upon binding to small biomolecules, ions, or changes in the membrane potential (Vm) are invaluable tools to study cellular signaling pathways. Here, we introduce a novel technique for simultaneous recording of multiple probes at millisecond time resolution: frequency- and spectrally-tuned multiplexing (FASTM). Different from present multiplexing approaches, FASTM uses phase-sensitive signal detection, which renders various combinations of common probes for Vm and ions accessible for multiplexing. Using kinetic stopped-flow fluorimetry, we show that FASTM allows simultaneous recording of rapid changes in Ca2+, pH, Na+, and Vm with high sensitivity and minimal crosstalk. FASTM is also suited for multiplexing using single-cell microscopy and genetically encoded FRET biosensors. Moreover, FASTM is compatible with optochemical tools to study signaling using light. Finally, we show that the exceptional time resolution of FASTM also allows resolving rapid chemical reactions. Altogether, FASTM opens new opportunities for interrogating cellular signaling.
Asunto(s)
Arbacia/fisiología , Técnicas Biosensibles/métodos , Colorantes Fluorescentes/química , Espermatozoides/fisiología , Animales , MasculinoRESUMEN
Enzymerhodopsins represent a recently discovered class of rhodopsins which includes histidine kinase rhodopsin, rhodopsin phosphodiesterases, and rhodopsin guanylyl cyclases (RGCs). The regulatory influence of the rhodopsin domain on the enzyme activity is only partially understood and holds the key for a deeper understanding of intra-molecular signaling pathways. Here, we present a UV-Vis and FTIR study about the light-induced dynamics of a RGC from the fungus Catenaria anguillulae, which provides insights into the catalytic process. After the spectroscopic characterization of the late rhodopsin photoproducts, we analyzed truncated variants and revealed the involvement of the cytosolic N-terminus in the structural rearrangements upon photo-activation of the protein. We tracked the catalytic reaction of RGC and the free GC domain independently by UV-light induced release of GTP from the photolabile NPE-GTP substrate. Our results show substrate binding to the dark-adapted RGC and GC alike and reveal differences between the constructs attributable to the regulatory influence of the rhodopsin on the conformation of the binding pocket. By monitoring the phosphate rearrangement during cGMP and pyrophosphate formation in light-activated RGC, we were able to confirm the M state as the active state of the protein. The described setup and experimental design enable real-time monitoring of substrate turnover in light-activated enzymes on a molecular scale, thus opening the pathway to a deeper understanding of enzyme activity and protein-protein interactions.
Asunto(s)
Blastocladiomycota/genética , GMP Cíclico/genética , Proteínas Fúngicas/genética , Guanilato Ciclasa/genética , Rodopsina/genética , Blastocladiomycota/metabolismo , GMP Cíclico/metabolismo , Proteínas Fúngicas/metabolismo , Guanilato Ciclasa/metabolismo , Rodopsina/metabolismo , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
The function of proteins is linked to their conformations that can be resolved with several high-resolution methods. However, only a few methods can provide the temporal order of intermediates and conformational changes, with each having its limitations. Here, we combine pulsed electron-electron double resonance spectroscopy with a microsecond freeze-hyperquenching setup to achieve spatiotemporal resolution in the angstrom range and lower microsecond time scale. We show that the conformational change of the Cα-helix in the cyclic nucleotide-binding domain of the Mesorhizobium loti potassium channel occurs within about 150 µs and can be resolved with angstrom precision. Thus, this approach holds great promise for obtaining 4D landscapes of conformational changes in biomolecules.
Asunto(s)
Electrones , Congelación , Mesorhizobium/química , Canales de Potasio/metabolismo , Modelos Moleculares , Canales de Potasio/química , Conformación Proteica , Análisis Espectral , Factores de TiempoRESUMEN
Enzymerhodopsins are a recently discovered class of natural rhodopsin-based photoreceptors with light-regulated enzyme activity. Currently, three different types of these fusion proteins with an N-terminal type-1 rhodopsin and a C-terminal enzyme domain have been identified, but their physiological relevance is mostly unknown. Among these, histidine kinase rhodopsins (HKR) are photo-regulated two-component-like signaling systems that trigger a phosphorylation cascade, whereas rhodopsin phosphodiesterase (RhoPDE) or rhodopsin guanylyl cyclase (RhGC) show either light-activated hydrolysis or production of cyclic nucleotides. RhGC, the best characterized enzymerhodopsin, is involved in the phototaxis of fungal zoospores and allows for optically controlled production of cyclic nucleotides in different cell-types. These photoreceptors have great optogenetic potential and possess several advantages over the hitherto existing tools to manipulate cyclic-nucleotide dynamics in living cells.
Asunto(s)
Biocatálisis , Enzimas/metabolismo , Optogenética/métodos , Rodopsina/metabolismo , Rodopsina/químicaRESUMEN
The cyclic nucleotides cAMP and cGMP are important second messengers that orchestrate fundamental cellular responses. Here, we present the characterization of the rhodopsin-guanylyl cyclase from Catenaria anguillulae (CaRhGC), which produces cGMP in response to green light with a light to dark activity ratio >1000. After light excitation the putative signaling state forms with τ = 31 ms and decays with τ = 570 ms. Mutations (up to 6) within the nucleotide binding site generate rhodopsin-adenylyl cyclases (CaRhACs) of which the double mutated YFP-CaRhAC (E497K/C566D) is the most suitable for rapid cAMP production in neurons. Furthermore, the crystal structure of the ligand-bound AC domain (2.25 Å) reveals detailed information about the nucleotide binding mode within this recently discovered class of enzyme rhodopsin. Both YFP-CaRhGC and YFP-CaRhAC are favorable optogenetic tools for non-invasive, cell-selective, and spatio-temporally precise modulation of cAMP/cGMP with light.
Asunto(s)
Adenilil Ciclasas/química , Blastocladiomycota/enzimología , AMP Cíclico/química , GMP Cíclico/química , Proteínas Fúngicas/química , Guanilato Ciclasa/química , Rodopsina/química , Adenilil Ciclasas/genética , Adenilil Ciclasas/metabolismo , Animales , Sitios de Unión , Blastocladiomycota/química , Blastocladiomycota/genética , Cristalización , AMP Cíclico/metabolismo , GMP Cíclico/metabolismo , Proteínas Fúngicas/metabolismo , Guanilato Ciclasa/genética , Guanilato Ciclasa/metabolismo , Modelos Moleculares , Unión Proteica , Dominios Proteicos , Ratas , Rodopsina/metabolismoRESUMEN
The cellular messenger cAMP regulates multiple cellular functions, including signaling in cilia and flagella. The cAMP dynamics in these subcellular compartments are ill-defined. We introduce a novel FRET-based cAMP biosensor with nanomolar sensitivity that is out of reach for other sensors. To measure cAMP dynamics in the sperm flagellum, we generated transgenic mice and reveal that the hitherto methods determining total cAMP levels do not reflect changes in free cAMP levels. Moreover, cAMP dynamics in the midpiece and principal piece of the flagellum are distinctively different. The sole cAMP source in the flagellum is the soluble adenylate cyclase (SACY). Although bicarbonate-dependent SACY activity requires Ca(2+), basal SACY activity is suppressed by Ca(2+). Finally, we also applied the sensor to primary cilia. Our new cAMP biosensor features unique characteristics that allow gaining new insights into cAMP signaling and unravel the molecular mechanisms underlying ciliary function in vitro and in vivo.
Asunto(s)
Técnicas Biosensibles/métodos , Cilios/química , AMP Cíclico/análisis , Flagelos/química , Animales , Línea Celular , Transferencia Resonante de Energía de Fluorescencia , Humanos , Masculino , Ratones Transgénicos , Sensibilidad y Especificidad , Espermatozoides/químicaRESUMEN
Component analysis of blood is a key diagnostic step in the detection of diseases. The separation of plasma from blood cells is therefore critical for the accuracy of diagnostic tests because cellular fractions can create discrepancies in analysis. The conventional method for separating the cellular fraction from whole blood is by centrifugation, which requires a laboratory infrastructure. In the last decade, intensive research to scale down experimental processes has seen unprecedented advances in microfabrication and related techniques that have led to utilization of the micro-level phenomenon to accomplish a myriad of physicochemical separation processes. Salient features of these devices include small sample size, faster reaction times, precise control of reaction environments, and affordability. Various plasma-separation devices have also been designed based on microfluidic platforms. The challenges associated with these devices are manifold: particle clogging, necessity for sample preparation, flow-rate maintenance, low reproducibility, and optimization of output. Further, quality, reliability, and consistency remain a huge issue with micromedical devices. The present article reviews current developments in the field of plasma separation from blood implementing innovative microtechnologies to achieve high-throughput plasma separation.