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Oncogene ; 35(33): 4368-78, 2016 08 18.
Article En | MEDLINE | ID: mdl-26804170

Bruton's tyrosine kinase (BTK) is essential for B-cell proliferation/differentiation and it is generally believed that its expression and function are limited to bone marrow-derived cells. Here, we report the identification and characterization of p65BTK, a novel isoform abundantly expressed in colon carcinoma cell lines and tumour tissue samples. p65BTK protein is expressed, through heterogeneous nuclear ribonucleoprotein K (hnRNPK)-dependent and internal ribosome entry site-driven translation, from a transcript containing an alternative first exon in the 5'-untranslated region, and is post-transcriptionally regulated, via hnRNPK, by the mitogen-activated protein kinase (MAPK) pathway. p65BTK is endowed with strong transforming activity that depends on active signal-regulated protein kinases-1/2 (ERK1/2) and its inhibition abolishes RAS transforming activity. Accordingly, p65BTK overexpression in colon cancer tissues correlates with ERK1/2 activation. Moreover, p65BTK inhibition affects growth and survival of colon cancer cells. Our data reveal that BTK, via p65BTK expression, is a novel and powerful oncogene acting downstream of the RAS/MAPK pathway and suggest that its targeting may be a promising therapeutic approach.


Cell Transformation, Neoplastic , Colonic Neoplasms/pathology , Protein-Tyrosine Kinases/physiology , ras Proteins/physiology , 5' Untranslated Regions/physiology , Agammaglobulinaemia Tyrosine Kinase , Cell Line, Tumor , Colonic Neoplasms/enzymology , Heterogeneous-Nuclear Ribonucleoprotein K/physiology , Humans , MAP Kinase Signaling System/physiology , Protein Isoforms/genetics , Protein Isoforms/physiology , Protein-Tyrosine Kinases/analysis , Protein-Tyrosine Kinases/genetics
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