Role of collagen and immunostaining for TGF-ß in the clinical and microscopic findings of pyogenic granuloma and peripheral ossifying fibroma.

BACKGROUND: Collagen is a component of Pyogenic Granuloma (PG) and Peripheral Ossifying Fibroma (POF) and performs different functions in these lesions. The objective of this study is to evaluate the role of collagen and immunostaining for Transforming Growth Factor beta (TGF-ß) in the clinical and microscopic findings of PG and POF. MATERIAL AND METHODS: PG (n=20) and POF (n=20) were selected for clinical evaluation (sex, age, localization, size and evolution time) and microscopic analysis (picrosirius red staining for collagen analysis and immunohistochemistry for TGF-ß) performed in the superficial and deep areas of the two lesions. ANOVA/Bonferroni and t-test, Pearson correlation and χ2 were used to compare the sites and parameters analyzed (p<0.05, GraphPad Prism 5.0). RESULTS: The depth of PG presented the highest amount of collagen (p<0.001), and its surface showed the lowest amount of type 1 collagen (yellow-red strong birefringence). Type 1 collagen gradually increased in depth of PG, surface and depth of POF (p<0.001). The number of TGF-ß+ cells was lower on the surface of PG compared with the depth of PG and the two areas of POF (p<0.001). Sex and localization did not affect these parameters, but the profile of collagen and immunostaining for TGF-ß suffered from modifications by the time of evolution and the size of the lesion. CONCLUSIONS: Although PG and POF are reactive gingival lesions, the expression of TGF-ß and its role in collagen showed different biological behaviors in these lesions, suggesting different biological origins for its components.

90 anos da constituição dos marcos legais da educação profissional em Enfermagem no Brasil / 90 years since the constitution of the legal frameworks of professional education in Nursing in Brazil

O objetivo da discussão proposta à mesa será apresentar o paralelo entre a formação técnica de nível médio em Enfermagem no Brasil e os tempos: político, normativo-legal e a produção científica. Justifica-se devido à carência de investigações sobre a temática e a necessidade de aprofundar as reflexões que permeiam essa formação de impacto quanti-qualitativo na saúde da população. Origina-se de estudos exploratórios prévios, dos quais emergiram três unidades: o panorama sócio-político, o movimento normativo-legal sobre a formação técnica em Enfermagem e a produção científica na área. Constatou-se múltiplas possibilidades de reflexões com implicações positivas para esse nível formativo (AU)