RESUMEN
An exopolysaccharide-producing bacterial strain GW4-15T, belonging to the genus Kaistella, was isolated from intertidal sediment from King George Island, Antarctic. The strain was Gram-stain-negative, aerobic, rod-shaped, non-motile and yellow-pigmented. The strain was able to grow in the presence of 0-2â% (w/v) NaCl (optimum, 0â%), at 4-30 °C (optimum, 20-28 °C) and at pH 5.0-10.0 (optimum, pH 8.0). A phylogenetic tree based on 16S rRNA gene sequences showed that strain GW4-15T formed a lineage within the genus Kaistella with the closest phylogenetic neighbours Kaistella carnis NCTC 13525T (98.3â%), Kaistella gelatinilytica G5-32T (97.7â%), Kaistella antarctica LMG 24720T (97.4â%) and Kaistella yonginensis HMD1043T (96.9â%). Digital DNA-DNA hybridization values of strain GW4-15T with K. carnis NCTC 13525T, K. antarctica LMG 24720T, K. gelatinilytica G5-32T and K. yonginensis HMD1043T were 22.8, 22.0, 21.7 and 21.6â%, respectively. The average nucleotide identity values between strain GW4-15T and K. carnis NCTC 13525T , K. antarctica LMG 24720T, K. gelatinilytica G5-32T and K. yonginensis HMD1043T were 79.3, 78.6, 77.5 and 77.2â%, respectively. The G+C content of the genome was 36.2âmol%. The major phospholipids were phosphatidylethanolamine and aminophospholipid. The predominant menaquinone was MK-6. The major fatty acids were anteiso-C15â:â0 (28.7â%), iso-C16â:â0 3-OH (15.7â%), iso-C16â:â0 H (10.0â%), iso-C16â:â0 (5.4â%), summed feature 9 (comprising iso-C17â:â1 ω9c and/or 10-methyl C16â:â0; 5.2â%) and iso-C15â:â0 (5.1â%). The monosaccharide composition of the new type of extracellular polymeric of GW4-15T was Glc, GalN, GlcN, Rha, Man and Gal with a molar ratio of 3.14â:â3.83â:â8.38â:â5.16â:â1â:â2.82. Based on phenotypic, phylogenetic and genotypic data, a novel species, Kaistella polysaccharea sp. nov., is proposed with the type strain GW4-15T (=CGMCC 1.19368T=KCTC 92753T).
Asunto(s)
Matriz Extracelular de Sustancias Poliméricas , Ácidos Grasos , Humanos , Masculino , Regiones Antárticas , Filogenia , ARN Ribosómico 16S/genética , Composición de Base , Ácidos Grasos/química , Análisis de Secuencia de ADN , ADN Bacteriano/genética , Técnicas de Tipificación BacterianaRESUMEN
A Gram-staining-positive, heterotrophic, non-spore-forming, non-motile, rod-shaped, strain ZS14-85T belonging to the genus Cryobacterium was isolated from soil in Antarctica. Growth was observed in the presence of 0-2% (w/v) NaCl, at pH 7.0-9.0 (optimum, pH 7.0) and 4-30 â (optimum, 20 â). Phylogenetic analysis showed that strain ZS14-85T formed a lineage in the genus Cryobacterium. The digital DNA-DNA hybridization (dDDH) values between strain ZS14-85T and its close relatives Cryobacterium psychrotolerans CGMCC 1.5382T, Cryobacterium soli MCCC 1K03549T and Cryobacterium breve NBRC 113800T were 22.5, 22.3 and 22.2%, respectively. Orthologous Average Nucleotide Identity (OrthoANI) scores between strain ZS14-85T and C. psychrotolerans CGMCC 1.5382T, C. breve NBRC 113800T and C. soli MCCC 1K03549T were 78.7, 78.1 and 77.7%, respectively. The polar lipids of strain ZS14-85T were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one unidentified glycolipid (GL) and two unidentified lipids (L). The major fatty acids were anteiso-C15:0 (60.7%), iso-C16:0 (17.0%) and anteiso-C17:0 (15.2%). MK-10, MK-11 and MK-9 were the predominant respiratory menaquinones. Based on phenotypic, phylogenetic and genotypic data, a novel species, Cryobacterium zhongshanensis sp. nov. is proposed. The type strain is ZS14-85T (= CCTCC AB 2019396T = KCTC 49384T).
Asunto(s)
Actinomycetales , Suelo , Regiones Antárticas , Filogenia , Análisis de Secuencia de ADN , Técnicas de Tipificación Bacteriana , ARN Ribosómico 16S/genética , Composición de Base , ADN Bacteriano/genética , Actinomycetales/genética , Ácidos Grasos/análisis , FosfolípidosRESUMEN
A Gram-staining-positive, non-spore-forming, non-flagellated, ellipsoidal, strain Z1-20 T belonging to the genus Arthrobacter was isolated from a soil sample collected from the Zhongshan station, Antarctic. Phylogenetic analysis of the 16S rRNA gene sequences and phylogenetic analysis revealed that strain Z1-20 T formed a unique single cluster in the genus Arthrobacter and shared high 16S rRNA sequence similarities of 97.1% and 96.9% with A. glacialis HLT2-12-2 T and A. psychrochitiniphilus GP3T, respectively. Values of Digital DNA-DNA hybridization (dDDH) between strain Z1-20 T against A. glacialis HLT2-12-2 T and A. psychrochitiniphilus GP3T were 20.3% and 13.8%, respectively. Average nucleotide identity (ANI) score between strain Z1-20 T against A. glacialis HLT2-12-2 T and A. psychrochitiniphilus GP3T were 72.5% and 72.1%, respectively. Genes for the synthesis of the osmoprotectant glycine betaine and higher copies of capA gene encoding cold shock protein were found in genome of Z1-20 T that may help Z1-20 T in cold-adaptation. Strain Z1-20 T comprised lysine as the diagnostic diamino acid. Based on the results of phylogenetic, phenotypic and chemotaxonomic features, strain Z1-20 T represents a novel species of a novel taxon of genus Arthrobacter, for which the name Arthrobacter terrae gen. nov., sp. nov. is proposed.
Asunto(s)
Actinobacteria , Arthrobacter , Actinobacteria/genética , Regiones Antárticas , Arthrobacter/metabolismo , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , ADN Bacteriano/metabolismo , Ácidos Grasos/metabolismo , Filogenia , ARN Ribosómico 16S/genética , ARN Ribosómico 16S/metabolismo , Análisis de Secuencia de ADN , SueloRESUMEN
A Gram-stain-negative, heterotrophic, aerobic, non-motile, rod-shaped bacterial strain (GW1-59T) belonging to the genus Lysobacter was isolated from coastal sediment collected from the Chinese Great Wall Station, Antarctica. The strain was identified using a polyphasic taxonomic approach. The strain grew well on Reasoner's 2A media and could grow in the presence of 0-4â% (w/v) NaCl (optimum, 1â%), at pH 9.0-11.0 and at 15-37 °C (optimum, 30 °C). Strain GW1-59T possessed ubiquinone-8 as the sole respiratory quinone. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were summed feature 9 (10-methyl C16â:â0 and/or iso-C17â:â1 ω9c), iso-C15â:â0, iso-C16â:â0, iso-C17â:â0, C16â:â0 and iso-C11â:â0 3-OH. DNA-DNA relatedness with Lysobacter concretionis Ko07T, the nearest phylogenetic relative (98.5â% 16S rRNA gene sequence similarity) was 23.4â% (21.1-25.9â%). The average nucleotide identity value between strain GW1-59T and L. concretionis Ko07T was 80.1â%. The physiological and biochemical results and low level of DNA-DNA relatedness suggested the phenotypic and genotypic differentiation of strain GW1-59T from other Lysobacter species. On the basis of phenotypic, phylogenetic and genotypic data, a novel species, Lysobacter antarcticus sp. nov., is proposed. The type strain is GW1-59T (=CCTCC AB 2019390T=KCTC 72831T).