Isolation of rfk-2 UV , a mutation that blocks spore killing by Neurospora Spore killer-3.

Neurospora Spore killer-3 ( Sk-3 ) is a selfish genetic element that kills spores to achieve gene drive. Here, we describe the isolation and mapping of rfk-2 UV , a mutation that disrupts spore killing. The rfk-2 UV mutation is located 15.6 cM from mus-52 on Chromosome III. The significance of this discovery with respect to Sk-3 evolution is discussed.