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The presence of antimicrobials in water has grown into a major global health concern. This study thus focused on the presence, ecological implications, and potential health risks associated with nine antimicrobials: five antibiotics (ampicillin, chloramphenicol, ciprofloxacin, metronidazole, and tetracycline) and four parabens (methylparaben, ethylparaben, propylparaben, and butylparaben) in surface water and groundwater samples collected from three Southwestern States in Nigeria (Osun, Oyo, and Lagos States). These antimicrobials were widely detected across the three States with ciprofloxacin being the most dominant having maximum average concentrations of 189 µg L-1 and 319 µg L-1 in surface water and groundwater respectively. The range of average concentrations of antibiotics in surface water are 47.3-235 µg L-1 (Osun), 27.9-166 µg L-1 (Oyo) and 52.1-159 µg L-1 (Lagos). For groundwater, it is 35.3-180 µg L-1 (Osun), 26.5-181 µg L-1 (Oyo) and 32.3-319 µg L-1 (Lagos). The average concentrations of all parabens were 32.4-153 µg L-1, 53.4-80.1 µg L-1, and 83.2-132 µg L-1 for surface water and 46.7-55.7 µg L-1, 53-117 µg L-1, and 62.4-118 µg L-1 for groundwater in Osun, Oyo, and Lagos States respectively. Methylparaben was most frequently detected paraben with average concentrations of 153 µg L-1 and 117 µg L-1 in surface water and groundwater respectively. The measured environmental concentrations of these antimicrobials pose a significant ecological risk while those of ciprofloxacin and ampicillin pose a high health risk to all population groups studied. The average concentrations of antibiotics investigated in this study exceeded their threshold values for Predicted No-Effect Concentrations (PNEC) associated with resistance selection, except for tetracycline.
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Agua Subterránea , Contaminantes Químicos del Agua , Parabenos/análisis , Agua , Nigeria , Medición de Riesgo , Antibacterianos/análisis , Tetraciclina , Ciprofloxacina , Ampicilina , Contaminantes Químicos del Agua/análisis , Monitoreo del AmbienteRESUMEN
This study provides, for the first time, data on the distribution and toxicity of catechol (CAT) and hydroquinone (HQ) in drinking water sources from Africa. Groundwater (boreholes and hand-dug wells) and surface water in three Southwestern States in Nigeria served as sampling sites. The concentrations of CAT and HQ in groundwater and surface water were determined throughout a period of 12 months, evaluating the effects of seasonal variation (rainy and dry seasons). Mean concentrations of CAT in water samples were higher than those of HQ. In this study, CAT was more frequently detected, with its mean concentration in groundwater samples higher in the rainy season (430 µg L-1) than in the dry season (175 µg L-1). Multivariate analysis using the Principal Component Analysis Software suggests that in most sample sites, CAT and HQ in water samples were from entirely different anthropogenic sources. The most impacted population groups were the toddlers and infants. Similarly, maximum and median concentrations of CAT in water samples pose serious risks to Daphnia at both acute and chronic levels. The results from this study suggest the need for further control of these dihydroxybenzenes through regular monitoring and removal from drinking water during treatment.
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This study reports the occurrence and risk assessment of 2,4-dinitrophenol (2,4-DNP), phenol (PHE), and 2,4,6-trichlorophenol (2,4,6-TCP) in drinking water sources in three south-western States in Nigeria (Osun, Oyo, and Lagos). Groundwater (GW) and surface water (SW) were collected during dry and rainy seasons of a year. The detection frequency of the phenolic compounds followed the trend Phenol > 2,4-DNP > 2,4,6-TCP. The mean concentrations of 2,4-DNP, Phenol, and 2,4,6-TCP in GW/SW samples from Osun State were 639/553 µg L-1, 261/262 µg L-1, and 169/131 µg L-1 during the rainy season and 154/7 µg L-1, 78/37 µg L-1, and 123/15 µg L-1 during the dry season, respectively. In Oyo State, the mean concentrations were 165/391 µg L-1 for 2,4-DNP and 71/231 µg L-1 for Phenol in GW/SW samples, respectively, during the rainy season. Generally, in the dry season, these values decreased. In any case, these concentrations are higher than those previously reported in water from other countries. The concentration of 2,4-DNP in water posed serious ecological risks to Daphnia on the acute scale while it was algae on the chronic scale. Estimated daily intake and hazard quotient calculations suggest that 2,4-DNP and 2,4,6-TCP in water pose serious toxicity concerns to humans. Additionally, the concentration of 2,4,6-TCP in water from Osun State in both seasons of the year and in both groundwater and surface water poses significant carcinogenic risks to persons ingesting water from these sources in the State. Every exposure group studied were at risk from ingesting these phenolic compounds in water. However, this risk decreased with increasing age of the exposure group. Results from the principal component analysis indicate that 2,4-DNP in water samples is from an anthropogenic source different from that for Phenol and 2,4,6-TCP. There is a strong need to treat water from GW and SW systems in these States before ingesting while assessing their quality regularly.
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Agua Potable , Agua Subterránea , Fenoles , Contaminantes Químicos del Agua , Humanos , 2,4-Dinitrofenol/análisis , Agua Potable/análisis , Monitoreo del Ambiente , Nigeria , Fenol/análisis , Fenoles/análisis , Medición de Riesgo , Contaminantes Químicos del Agua/análisisRESUMEN
The aquatic environment is a hotspot for the transfer of antibiotic resistance to humans and animals. Several reviews have put together research efforts on the presence and distribution of antibiotic resistant bacteria (ARB), antibiotic resistance genes (ARGs), and antibiotic chemical residue (ACRs) in food, hospital wastewater, and even in other aquatic environments. However, these reports are largely focused on data from developed countries, while data from developing countries and especially those in Africa, are only marginally discussed. This review is the first effort that distills information on the presence and distribution of ARGs and ACRs in the African aquatic environments (2012-2021). This review provides critical information on efforts put into the study of ARB, ARGs, and ACRs in aquatic environments in Africa through the lens of the different sub-regions in the continent. The picture provided is compared with those from some other continents in the world. It turns out that the large economies in Africa (South Africa, Nigeria, Tunisia, Kenya) all have a few reports of ARB and ARGs in their aquatic environment while smaller economies in the continent could barely provide reports of these in their aquatic environment (in most cases no report was found) even though they have some reports on resistomes from clinical studies. Interestingly, the frequency of these reports of ARB and ARGs in aquatic environments in Africa suggests that the continent is ahead of the South American continent but behind Europe and Asia in relation to providing information on these contaminants. Common ARGs found in African aquatic environment encode resistance to sulfonamide, tetracycline, ß-lactam, and macrolide classes of antibiotics. The efforts and studies from African scientists in eliminating ARB and ARGs from the aquatic environment in Africa are also highlighted. Overall, this document is a ready source of credible information for scientists, policy makers, governments, and regional bodies on ARB, ARGs, and ACRs in aquatic environments in Africa. Hopefully, the information provided in this review will inspire some necessary responses from all stakeholders in the water quality sector in Africa to put in more effort into providing more scientific evidence of the presence of ARB, ARGs, and ACRs in their aquatic environment and seek more efficient ways to handle them to curtail the spread of antibiotic resistance among the population in the continent. This will in turn, put the continent on the right path to meeting the United Nations Sustainable Development Goals #3 and #6, which at the moment, appears to be largely missed by most countries in the continent.
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Genes Bacterianos , Aguas Residuales , Antagonistas de Receptores de Angiotensina , Inhibidores de la Enzima Convertidora de Angiotensina , Animales , Antibacterianos , Bacterias/genética , Humanos , Macrólidos , Nigeria , Sulfonamidas , Tetraciclinas , Aguas Residuales/análisis , beta-LactamasRESUMEN
Parabens are chemicals extensively used in pharmaceuticals, cosmetics, personal hygiene and food products as preservatives. They are classified as emerging contaminants with endocrine-disrupting capability. In this study, the concentrations of Methylparaben (MeP), Ethylparaben (EtP), Propylparaben (PrP) and Butylparaben (BuP) were obtained from groundwater, surface-water and packaged water samples collected from urban and rural areas of Osun State, Nigeria using HPLC-UV equipment. Data obtained were subjected to descriptive (Mean ± SD), inferential (Kruskal-Wallis test) and multivariate analyses. MeP had the highest average concentration of 163 and 68 µg L-1 in surface water and groundwater respectively while concentrations of MeP, EtP, PrP and BuP were higher than previously reported in other countries. Methylparaben had the highest detection frequencies (88.0 and 50.0%) followed by BuP (69.0 and 50.0%) in surface water and groundwater respectively. No significant difference was observed for concentrations of parabens in groundwater samples in urban and rural sampling sites, suggesting that people living around these sites are equally exposed to any health implications from the use of paraben-polluted potable water. Principal Component Analysis (PCA) data suggest that the pairs MeP & EtP, PrP & BuP (in surface water samples) and MeP, EtP, & PrP (in groundwater samples) are from similar pollution sources. Ecological risk assessment using Algae, Fish, and Daphnia suggests Daphnia as the most sensitive organism while BuP and PrP show the highest health risk. Human exposure assessment showed that higher overall median estimated daily intake (EDI) values for groundwater were observed in infants (1.71 µg kg-1 bw day-1, ∑PBs) compared to toddlers (1.03 µg kg-1 bw day-1, ∑PBs), children (0.64 µg kg-1 bw day-1, ∑PBs), teenagers (0.51 µg kg-1 bw day-1, ∑PBs) and adults (0.62 µg kg-1 bw day-1, ∑PBs). Although these values are below limits set in a few countries, potential bioaccumulation could lead to severe health consequences.
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Cosméticos , Parabenos , Adolescente , Animales , Exposición a Riesgos Ambientales/análisis , Humanos , Nigeria , Parabenos/análisis , Conservadores Farmacéuticos , AguaRESUMEN
Parabens are biocides used as preservatives in food, cosmetics and pharmaceuticals. They possess antibacterial and antifungal activity due to their ability to disrupt cell membrane and intracellular proteins, and cause changes in enzymatic activity of microbial cells. Water, one of our most valuable natural resource, has become a huge reservoir for parabens. Halogenated parabens from chlorination/ozonation of water contaminated with parabens have shown to be even more persistent in water than other types of parabens. Unfortunately, there is dearth of data on their (halogenated parabens) presence and fate in groundwater which serves as a major source of drinking water for a huge population in developing countries. An attempt to neglect the presence of parabens in water will expose man to it through ingestion of contaminated food and water. Although there are reviews on the occurrence, fate and behaviour of parabens in the environment, they largely omit toxicity and removal aspects. This review therefore, presents recent reports on the acute and chronic toxicity of parabens, their estrogenic agonistic and antagonistic activity and also their relationship with antimicrobial resistance. This article further X-rays several techniques that have been employed for the removal of parabens in water and their drawbacks including adsorption, biodegradation, membrane technology and advanced oxidation processes (AOPs). The heterogeneous photocatalytic process (one of the AOPs) appears to be more favoured for removal of parabens due to its ability to mineralize parabens in water. However, more work is needed to improve this ability of heterogeneous photocatalysts. Perspectives that will be relevant for future scientific studies and which will drive policy shift towards the presence of parabens in our drinking waters are also offered. It is hoped that this review will elicit some spontaneous actions from water professionals, scientists and policy makers alike that will provide more data, effective technologies, and adaptive policies that will address the growing threat of the presence of parabens in our environment with respect to human health.
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Cosméticos , Purificación del Agua , Humanos , Parabenos/toxicidad , Conservadores Farmacéuticos , AguaRESUMEN
Studies on earthworms using molecular markers are rare in Africa except a handful from South Africa. Reports on Libyodrilus violaceous, an earthworm found in West Africa are available including their metal tolerance and bioaccumulation capacity but their molecular characterization and ecotoxicology studies are scarce. In this study, triplicate L. violaceous specimens were collected from four locations within a petroleum polluted site and one in a control site, ≃1Km away from point of spill. DNA was extracted and 18S rRNA and 16S rRNA genes were amplified and sequenced. DNA methylation of their 18S rRNA gene was determined using Methylation specific PCR (MSP) method. Phylogenetic trees generated for 18S rRNA and 16S rRNA genes grouped L. violaceous within the Eudrilidae family concurrent with its conventional grouping and MSP results indicate no methylation in L. violaceous population from this site.
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OBJECTIVE: To investigate the effects of different extracts of Anthocleista djalonensis on the testis and epididymal sperms of rats. METHODS: Fifty male Wistar rats were randomly divided into 10 groups (n=5 in each group) and orally treated with 50, 100 and 200 mg/kg body weight each of methanol, aqueous ethanol (H-EtOH) and chloroform extracts of A. djalonensis. Corn oil was used as vehicle (2 mL/kg). After 60 days of treatment, testosterone (T) and cholesterol (CHOL) concentrations, catalase (CAT), lactate dehydrogenase (LDH), 3ß-hydroxysteroid dehydrogenase (3ß-HSD) and superoxide dismutase (SOD) activities in the testes along with myeloperoxidase (MPO) activities and nitrite concentrations (NO) in the serum and testes as well as sperm quality were measured. RESULTS: T and CHOL concentrations along with 3ß-HSD activity were significantly higher in the animals treated with the low dose than in those treated with the high dose of the chloroform extract (P<0.05). Furthermore, the chloroform extract was more effective than the methanol extract that had the most marginal effect on T level at the high dose and the H-EtOH extract that was only effective at the medium dose. LDH activity was dose-dependently increased by the extracts in all groups. The CAT-SOD antioxidant system was increased in the treated animals at all doses compared to the control values, but the increase in glutathione level reached significant level in those treated with the low dose H-EtOH aqueous ethanol extract (P<0.05). Only the high dose of chloroform extract had significant inhibitory effects on MPO activity (P<0.05). Serum NO concentration was decreased at all doses of the extracts. The inhibitory effects of the extracts on testicular NO concentrations follow this order, chloroform extract > H-EtOH > methanol. Although all extracts at all doses showed excellent stimulatory effects on sperm quality (count, motility and morphology), the methanol extract at the high dose was the most effective on sperm count (P<0.05). CONCLUSION: The chloroform extract of A. djalonensis has better androgen-like and anti-inflammatory effects whereas the methanol extract has the best effect on sperm count.
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Antiinflamatorios/farmacología , Antioxidantes/farmacología , Extractos Vegetales/farmacología , Espermatozoides/efectos de los fármacos , Testículo/efectos de los fármacos , Animales , Masculino , Nigeria , Raíces de Plantas , Ratas , Ratas Wistar , Recuento de Espermatozoides , Testosterona/metabolismoRESUMEN
This study reports a new class of photocatalytic hybrid clay nanocomposites prepared from low-cost sources (kaolinite clay and Carica papaya seeds) doped with Zn and Cu salts via a solvothermal process. X-ray diffraction analysis suggests that Cu-doping and Cu/Zn-doping introduce new phases into the crystalline structure of Kaolinite clay, which is linked to the reduced band gap of kaolinite from typically between 4.9 and 8.2 eV to 2.69 eV for Cu-doped and 1.5 eV for Cu/Zn hybrid clay nanocomposites (Nisar, J.; Århammar, C.; Jämstorp, E.; Ahuja, R. Phys. Rev. B 2011, 84, 075120). In the presence of solar light irradiation, Cu- and Cu/Zn-doped nanocomposites facilitate the electron-hole pair separation. This promotes the generation of singlet oxygen which in turn improves the water disinfection efficiencies of these novel nanocomposite materials. The nanocomposite materials were further characterized using high-resolution scanning electron microscopy, fluorimetry, thermogravimetric analysis, and Raman spectroscopy. The breakthrough times of the nanocomposites for a fixed bed mode of disinfection of water contaminated with 2.32 × 107 cfu/mL E. coli ATCC 25922 under solar light irradiation are 25 h for Zn-doped, 30 h for Cu-doped, and 35 h for Cu/Zn-doped nanocomposites. In the presence of multidrug and multimetal resistant strains of E. coli, the breakthrough time decreases significantly. Zn-only doped nanocomposites are not photocatalytically active. In the absence of light, the nanocomposites are still effective in decontaminating water, although less efficient than under solar light irradiation. Electrostatic interaction, metal toxicity, and release of singlet oxygen (only in the Cu-doped and Cu/Zn-doped nanocomposites) are the three disinfection mechanisms by which these nanocomposites disinfect water. A regrowth study indicates the absence of any living E. coli cells in treated water even after 4 days. These data and the long hydraulic times (under gravity) exhibited by these nanocomposites during photodisinfection of water indicate an unusually high potential of these nanocomposites as efficient, affordable, and sustainable point-of-use systems for the disinfection of water in developing countries.
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Petroleum contamination is a significant contributor of elevated level of toxic heavy metals, which are of great concern to human health, due to their non-biodegradable nature. Agaye community has experienced frequent gasoline spills due to pipeline vandalisation, resulting in the contamination of soil and water sources. The concentrations of metals (Cd, Cr, Cu, Mn, Ni, Pb, V and Zn) in groundwater, surface-water and soil were determined from a total of 216 samples acquired bi-monthly for two years by Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) to evaluate the impact of oil spills. Multivariate analyses using principal component analysis (PCA) and cluster analysis (CA) were also used to study the interactions between metals and identify the possible sources of contamination. The concentrations of heavy metals in soil and water samples (surface and groundwater) were in decreasing order of Mnâ¯>â¯Niâ¯>â¯Znâ¯>â¯Cuâ¯>â¯Vâ¯>â¯Crâ¯>â¯Pbâ¯>â¯Cd and Niâ¯>â¯Znâ¯>â¯Vâ¯>â¯Cuâ¯>â¯Mnâ¯>â¯Pbâ¯>â¯Crâ¯>â¯Cd respectively. Ni concentration ranged from 0.42-8.05â¯mgâ¯kg-1 and 0.10-2.85â¯mgâ¯L-1 for soil and groundwater respectively. Ni and V were more enhanced (Pâ¯<â¯0.05) in soil samples. This study showed that there was significant relationship between elevated levels of Cr, Cu, Ni and Zn and oil spillage, due to petroleum spills and that residents were vulnerable to and at greater risk of non-carcinogenic hazards if they consumed groundwater. Multivariate analyses showed significant anthropogenic intrusions of two diagnostic heavy metals (Ni and V) for petroleum contamination in the soils and water sources.
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Landfill sites near human settlements are known to have adverse health effects. Here, we investigated the effect of different concentrations of leachates from the Redemption Camp landfill (RCLL, 10%, 30%, 50%) on the liver of adult female rats after 21 days of exposure in their drinking water. The physicochemical and metal analyses showed that biochemical oxygen and chemical oxygen demand, zinc and magnesium levels were significantly high, whereas copper level was low in RCLL when compared to water samples from residential areas close to the landfill site, and were higher than the acceptable limits (pâ¯<â¯0.05). The predominant bacteria isolates recovered from the leachate and drinking water samples were Escherichia coli, Salmonella spp and Shigella spp. At the end of the 21-day exposure, RCLL increased the weight of the liver. Malondialdehyde concentrations were increased and glutathione levels were decreased significantly in the liver of treated animals at all concentrations of leachates tested. Furthermore, the activities of serum alanine amino transferase, aspartate amino transferase, gamma glutamyl transferase and cholesterol concentrations were increased whereas bilirubin and albumin levels were decreased dose-dependently. Histological examination of the liver was characterized by accumulation of inflammatory cells around hepatocytes, and extended sinusoids. The histo-pathological alterations and oxidative damage observed in the liver of treated rats and occurrence of pathogenic species and metals in the RCLL may suggest possible impaired hepatic health in subjects with occupational or environmental exposure.
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Enfermedad Hepática Inducida por Sustancias y Drogas/etiología , Metales Pesados/análisis , Contaminantes Químicos del Agua/análisis , Animales , Agua Potable/análisis , Agua Potable/microbiología , Femenino , Glutatión/análisis , Inflamación , Hígado/química , Hígado/enzimología , Hígado/patología , Malondialdehído/análisis , Nigeria , Estrés Oxidativo , Ratas , Transaminasas/análisis , Instalaciones de Eliminación de Residuos , Contaminantes Químicos del Agua/toxicidadRESUMEN
Xylopia aethiopica (Annonaceae) is used in some folk medicines and widely consumed as a spice in some parts of Nigeria. Its efficacy as an anti-androgenic substance has warranted the attention of African scholars. This study evaluated the enzymatic activity of lactate dehydrogenase (LDH), γ-glutamyl transferase (γ-GT), sperm quality (motility, count, morphology), testosterone level and histo-pathological changes of the testis of rats chronically treated with ethanolic extract of the pods (without seeds), seeds, and fruits (pods + seeds) of Xylopia aethiopica. Male Wistar (224-246 g) rats were treated with the extract of the pods, seeds, and fruits of Xylopia aethiopica at the dose of 0, 50, 100, and 200 mg/kg body wt. for 60 days. Serum biochemistry, sperm quality and histo-pathological examination of the testis were assessed for any treatment-related adverse effects. After treatment with Xylopia aethiopica, testosterone level was decreased dose-dependently in the animals treated with the seed extract compared to all other groups. The enzymatic activities of LDH and γ-GT were higher in rats treated with the seed and fruit extracts compared with those treated with the pods. The numbers of motile sperm, and counts were decreased while the numbers of sperm with morphological defects were higher in rats treated with the seed and fruit extracts compared to the control. Histopathological changes of the testis were also more severe in rats treated with the highest dose of the seed extract. We conclude that the compounds related to the anti-infertility effects of Xylopia aethiopica are present in the seeds.