Influence of triazines and lipopolysaccharide coexposure on inflammatory response and histopathological changes in the testis and liver of BalB/c mice.

Background: Triazines are environmental active chemicals that have been reported to alter the inflammatory status of the gonads. We tested the anti-inflammatory effect of the triazines (atrazine; ATZ, simazine; SMZ and cyanazine; CYZ) on the testis and compared it with the more classical liver model that has substantial populations of resident macrophages comparable to the testis. Methods: BalB/c mice were treated with 25 mg/kg ATZ, SMZ and CYZ for 30 days and injected with lipopolysaccharide (0.5 mg/kg i.p.) 6 h before sacrifice. Myeloperoxidase activity and nitric oxide level in the testis and liver homogenates were determined by spectrophotometry whereas tumor necrosis factor-alpha and interleukin-6 concentrations were evaluated by immunoassay. Haematoxylin and eosin stained sections of the tissues were observed using a light microscope. Results: Myeloperoxidase activity, nitric oxide, tumor necrosis factor-alpha, and interleukin-6 levels were decreased in the liver and testis of the triazines co-treated animals. SMZ has the most potent inhibitory effect and ATZ the least effect on inflammatory mediators in both tissues. Microscopic evaluation showed loss of inflammatory cells in the inter-tubular areas of the testis and few patchy masses of infiltrating inflammatory cells around the central vein of the liver. Conclusion: Triazines inhibit the levels of inflammatory mediators in the testis and liver of mice. The anti-inflammatory effect of triazines in a lipopolysaccharide-induced inflammation model was established in this study.

Effect of Fe and Cd Co-Exposure on Testicular Steroid Metabolism, Morphometry, and Spermatogenesis in Mice.

The mechanism of testicular toxicity of simultaneous multiple exposures to metals is poorly understood. Previous studies reported that the toxic effect of cadmium (Cd) is modified by tissue concentration of iron (Fe). Using the mice (Mus musculus) model in the present study, we demonstrated that combined Cd (25 mg kg-1 bw) and Fe (100 mg kg-1 bw) treatment increased both Cd and Fe testicular concentrations much more than separate exposures to either of the metals. Intratesticular Cd and Fe concentrations were inversely correlated (r = - 0.731, p < 0.05) on administration of Fe but not on combined exposure to both metals when they were positively correlated (versus Cd; r = 0.793, versus Fe; r = 0.779, p < 0.05). Additionally, Cd + Fe treatment increased testicular lipid peroxidation and depleted intratestesticular testosterone, cholesterol and glutathione concentrations much more than their separate treatment. This was also associated with decreased activity of the germ cell marker, testicular lactate dehydrogenase, and increased testicular myeloperoxidase activity. These changes resulted in decreased seminiferous epithelial height, tubular diameter, germ cell (spermatogonia, spermatocytes, and spermatids) numbers, and severe tissue damage. In conclusion, Cd + Fe intake have synergistic toxic effects on testicular steroid formation and spermatogenesis due to the high testicular concentrations of both metals.

Oral administration of cadmium depletes intratesticular and epididymal iron levels and inhibits lipid peroxidation in the testis and epididymis of adult rats.

Cadmium (Cd)-induced tissue injury depends on the accumulated Cd which differentially affects endogenous iron (Fe). To investigate this, adult rats were treated by oral gavage with Cd (50 mg/kg body wt.) once a week for 15, 30 and 60 days and sacrificed a day after last administration. After the 15th and 30th day of treatment, Cd had no effect on thiobarbituric acid reactive substances (TBARS) and endogenous Fe levels but exhibited anti-androgenic effects (p < 0.05) and caused histological damages. At day 60, Cd was accumulated by 156.30% and 364.77% above control values at concentrations that decreased endogenous Fe levels by 46.41% and 50.31% in the testis and epididymis respectively. The histological damages were characterized by decreased tubular diameter, damage to the epithelium leading to loss of tubular germ cells and absent of spermatozoa in the epididymal lumen. Although myeloperoxidase activities were increased, TBARS levels were found to decrease significantly at day 60 in the serum, testis and epididymis suggesting that the histological damages were not caused by lipid peroxidation. Furthermore, TBARS correlated negatively with Cd in the testis (r = -0.251, p < 0.05) and epididymis (r = -0.286, p < 0.05); Fe correlated positively with TBARS in the testis (r = +0.217, p < 0.05) and Cd correlated negatively with Fe in the testis (r = -0.461, p < 0.05) and epididymis (r = -0.109, p < 0.05). The antioxidant enzymes, superoxide dismutase and glutathione peroxidase were also decreased in the gonads after 60 days Cd treatment. Overall, anti-androgenic effects and histo-pathological changes are early indicators of direct effects of Cd and occur before decrease in TBARS which is secondarily related to the modifying of Fe contents.

Trace metal contents of selected seeds and vegetables from oil producing areas of Nigeria.

The concentrations of accumulated trace metals in selected seeds and vegetables collected in the oil producing Rivers State of Nigeria were investigated. The values were compared with those of seeds and vegetables cultivated in Owerri, a less industrialized area in Nigeria. The lead (Pb) and cadmium (Cd) contents of the seeds obtained from Rivers State ranged between 0.10 and 0.23 microg/g dry weight, while those of the seeds cultivated in Owerri fell below the detection limit of 0.01 microg/g dry weight. The highest manganese (Mn) level (902 microg/g dry weight) was found in Irvingia garbonesis seeds cultivated in Rivers State. Similarly, the highest nickel (Ni) value (199 microg/g dry weight) was also obtained in I. garbonesis, however, in the seeds sampled in Owerri. The highest copper (Cu), zinc (Zn), and iron (Fe) levels (16.8, 5.27, and 26.2 microg/g dry weight, resp.) were detected in seeds collected in Rivers State. With the exception of Talinum triangulae, Ocinum gratissimum, and Piper guineese, with Pb levels of 0.09, 0.10, and 0.11 microg/g dry weight, respectively, the Pb and Cd levels in the vegetables grown in Owerri fell below the detection limit of 0.01 microg/g dry weight. The trace metal with the highest levels in all the vegetables studied was Mn, followed by Fe. The highest concentrations of Ni and Cu occurred in vegetables collected from Rivers State, while the highest level of Zn was observed in Piper guineese collected in Owerri, with a value of 21.4 microg/g dry weight. Although the trace metal concentrations of the seeds and vegetables collected in Rivers State tended to be higher than those of the seeds and vegetables grown in Owerri, the average levels of trace metals obtained in this study fell far below the WHO specifications for metals in foods.