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AbstractTemperate reptiles are often considered to be low-energy systems, with their discrete use of time and energy making them model systems for the study of time-energy budgets. However, the semifrequent replacement and sloughing of the epidermis is a ubiquitous feature of squamate reptiles that is often overlooked when accounting for time and energy budgets in these animals. We used open-flow respirometry to measure both the energetic effort of ecdysis and the duration of the associated metabolic upregulation (likely related to behavioral changes often reported for animals in shed) in wild-caught timber rattlesnakes (Crotalus horridus). We hypothesized that total effort of skin biosynthesis and physical removal would be related to body mass and expected the duration of the process to remain static across individuals at a fixed temperature (25°C). We provide both the first measurements of the cost of skin biosynthesis and physical removal in a reptile and the highest-resolution estimate of process duration recorded to date. We found that skin biosynthesis, but not the cost of physical removal of the epidermis, was related to body mass. Shed cycle duration was consistent across individuals, taking nearly 4 wk from process initiation to physical removal of the outermost epidermal layer. Total energetic effort of ecdysis was of sizeable magnitude, requiring â¼3% of the total annual energy budget of a timber rattlesnake. Energetic effort for a 500-g snake was equivalent to the amount of metabolizable energy acquired from the consumption of approximately two adult mice. Ecdysis is a significant part of the time-energy budgets of snakes, necessitating further attention in studies of reptilian energetics.
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Crotalus , Metabolismo Energético , Muda , Animales , Metabolismo Energético/fisiología , Crotalus/metabolismo , Muda/fisiología , Masculino , FemeninoRESUMEN
This research aimed to conduct a cost-benefit analysis of a volunteer program. The literature lacked empirical evidence on the cost efficiency of voluntary work in the public-sector context. Most previous studies have paid attention to the cases of nonprofit organizations, although there have been many volunteer programs in public organizations. In this research, we focused on the United States Coast Guard Auxiliary Boat Crew Program which may help distressed boaters in distress, patrol regattas and marine events, and assist in maritime observations. We discovered that approximately $1.39 million was invested in volunteers to run the boat crew program for 2019. We also estimated the total monetary benefit of volunteer contributions by using the replacement cost method. As a result, we found that 5369 volunteers contributed approximately 199,000 h to the boat crew program in 2019. It means that the program extended the equivalent of 104 full-time Coast Guard personnel in 2019. The total economic benefit attributed to the volunteer contributions of labor ranged from $4.8 million to $5.4 million. Therefore, for every dollar the Coast Guard invested in the volunteer program, they obtained an additional return of $3.42 to $3.89 from volunteer contributions.
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Personal Militar , Navíos , Análisis Costo-Beneficio , Gobierno , Humanos , Evaluación de Programas y Proyectos de Salud , Estados Unidos , VoluntariosRESUMEN
AbstractAccelerated extinction rates have prompted an increased focus on the interplay between environmental change and species response. The effects of environmental change on thermal opportunity are typically considered through a climate change context. However, habitat alteration can also have strong effects on the thermal environment. Additionally, habitat alteration is considered a leading factor of species extinction, yet few studies address the influence of habitat alteration on thermal opportunity and time-energy budgets in at-risk species. Here, we show the strong effects that habitat degradation can have on thermal opportunity, time-energy budgets, and life history demographics of local populations. In the Ozark Mountains of northern Arkansas, woody vegetation encroachment has resulted in a shift in life history traits that appears to play an important role in recent extirpations of eastern collared lizards (Crotaphytus collaris). Populations in degraded habitats experienced a decline in thermal opportunity and less time at body temperatures (time at Tb) suitable for digestion compared with those in intact habitats. We used our data to model the effect of reduced time at Tb on the net assimilated energy available for growth and reproduction. Our model predicts an â¼46% decline in the annual fecundity of individuals, which is similar to empirical observations of reproduction of C. collaris populations in degraded habitats (~49%). We conclude that C. collaris in degraded habitats experienced reduced growth and reproduction primarily as a result of constrained thermal opportunity leading to a decline in digestive processing rates. Our study applies an underappreciated approach to identify the biophysical and time-energy effects of habitat alteration.
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Ecosistema , Lagartos , Animales , Temperatura Corporal , Cambio Climático , Extinción Biológica , Humanos , TemperaturaRESUMEN
Negative drag coefficients are normally associated with a vessel outfitted with a sail to extract energy from the wind and propel the vehicle forward. Therefore, the notion of a heavy vehicle, that is, a semi truck, that generates negative aerodynamic drag without a sail or any external appendages may seem implausible, especially given the fact that these vehicles have some of the largest drag coefficients on the road today. However, using both wind tunnel measurements and computational fluid dynamics simulations, we demonstrate aerodynamically integrated vehicle shapes that generate negative body-axis drag in a crosswind as a result of large negative frontal pressures that effectively "pull" the vehicle forward against the wind, much like a sailboat. While negative body-axis drag exists only for wind yaw angles above a certain analytical threshold, the negative frontal pressures exist at smaller yaw angles and subsequently produce body-axis drag coefficients that are significantly less than those of modern heavy vehicles. The application of this aerodynamic phenomenon to the heavy vehicle industry would produce sizable reductions in petroleum use throughout the United States.
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We demonstrate an additive manufacturing approach to produce gradient refractive index glass optics. Using direct ink writing with an active inline micromixer, we three-dimensionally print multimaterial green bodies with compositional gradients, consisting primarily of silica nanoparticles and varying concentrations of titania as the index-modifying dopant. The green bodies are then consolidated into glass and polished, resulting in optics with tailored spatial profiles of the refractive index. We show that this approach can be used to achieve a variety of conventional and unconventional optical functions in a flat glass component with no surface curvature.
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Various types of embolization devices have been developed for the treatment of cerebral aneurysms. However, it is challenging to properly evaluate device performance and train medical personnel for device deployment without the aid of functionally relevant models. Currentin vitroaneurysm models suffer from a lack of key functional and morphological features of brain vasculature that limit their applicability for these purposes. These features include the physiologically relevant mechanical properties and the dynamic cellular environment of blood vessels subjected to constant fluid flow. Herein, we developed three-dimensionally (3D) printed aneurysm-bearing vascularized tissue structures using gelatin-fibrin hydrogel of which the inner vessel walls were seeded with human cerebral microvascular endothelial cells (hCMECs). The hCMECs readily exhibited cellular attachment, spreading, and confluency all around the vessel walls, including the aneurysm walls. Additionally, thein vitroplatform was directly amenable to flow measurements via particle image velocimetry, enabling the direct assessment of the vascular flow dynamics for comparison to a 3D computational fluid dynamics model. Detachable coils were delivered into the printed aneurysm sac through the vessel using a microcatheter and static blood plasma clotting was monitored inside the aneurysm sac and around the coils. This biomimeticin vitroaneurysm model is a promising method for examining the biocompatibility and hemostatic efficiency of embolization devices and for providing hemodynamic information which would aid in predicting aneurysm rupture or healing response after treatment.
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Bioimpresión , Embolización Terapéutica , Aneurisma Intracraneal , Prótesis Vascular , Células Endoteliales , Humanos , Aneurisma Intracraneal/terapiaRESUMEN
Lipins are eukaryotic proteins with functions in lipid synthesis and the homeostatic control of energy balance. They execute these functions by acting as phosphatidate phosphatase enzymes in the cytoplasm and by changing gene expression after translocation into the cell nucleus, in particular under fasting conditions. Here, we asked whether nuclear translocation and the enzymatic activity of Drosophila Lipin serve essential functions and how gene expression changes, under both fed and fasting conditions, when nuclear translocation is impaired. To address these questions, we created a Lipin null mutant, a mutant expressing Lipin lacking a nuclear localization signal (LipinΔNLS ), and a mutant expressing enzymatically dead Lipin. Our data support the conclusion that the enzymatic but not nuclear gene regulatory activity of Lipin is essential for survival. Notably, adult LipinΔNLS flies were not only viable but also exhibited improved life expectancy. In contrast, they were highly susceptible to starvation. Both the improved life expectancy in the fed state and the decreased survival in the fasting state correlated with changes in metabolic gene expression. Moreover, increased life expectancy of fed flies was associated with a decreased metabolic rate. Interestingly, in addition to metabolic genes, genes involved in feeding behavior and the immune response were misregulated in LipinΔNLS flies. Altogether, our data suggest that the nuclear activity of Lipin influences the genomic response to nutrient availability with effects on life expectancy and starvation resistance. Thus, nutritional or therapeutic approaches that aim at lowering nuclear translocation of lipins in humans may be worth exploring.
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Núcleo Celular/metabolismo , Drosophila , Ayuno , Regulación de la Expresión Génica , Transporte Activo de Núcleo Celular , Animales , Compuestos Orgánicos/metabolismo , Análisis de SupervivenciaRESUMEN
Information on bioenergetics can provide valuable insight into the ecology, life history and population dynamics of organisms. For ectothermic animals, thermal sensitivity of digestion is an important determinant of net assimilated energy budgets. A recent study in the Ozark Mountains indicated that eastern collared lizards (Crotaphytus collaris) restricted to encroached glades (characterized by woody vegetation encroachment) experience reduced environmental heat loads and have reduced age-specific growth and reproductive rates compared to populations in intact glades. To assess the potential impact of reduced body temperatures on assimilation rates of C. collaris in encroached glades, we conducted feeding trials across four temperature treatments (28, 31, 34 and 37°C). We tested for temperature effects on voluntary feeding rates, passage times, apparent assimilated energy (AE) and metabolizable energy (ME). Passage times decreased and voluntary feeding rates increased significantly with increasing temperature. Consumption explained the majority of variance in AE and ME, followed by the effect of temperature treatments. Using data on voluntary feeding rates, passage times and ME as a function of temperature, we estimated over a 10-fold increase in predicted daily assimilated energy across temperature treatments (28°C = 0.58 kJ/day, 31°C = 1.20 kJ/day, 34°C = 4.30 kJ/day, 37°C = 7.95 kJ/day). Thus, lower heat loads in encroached glades may cause reduced body temperature and result in restricted energy assimilation rates. Our study provides a novel approach to the integration of bioenergetics and conservation and shows the efficacy of using information on digestive performance to investigate underlying mechanisms in a conservation context.
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To prevent aneurysmal rupture, intracranial aneurysms are often treated with endovascular metal coils that fill the aneurysm sac and stimulate thrombus formation, thereby isolating the aneurysm from the arterial flow. Despite its widespread use, this method can result in suboptimal outcomes leading to aneurysm recurrence. Recently, shape memory polymer foam has been proposed as an alternative aneurysm filler. In this work, a computational thrombus model is used to predict the clotting response within idealized 2D aneurysms virtually treated with foam. The results are compared to previously reported clot formation predictions in identical 2D aneurysm geometries filled with simplified endovascular metal coil shapes. Each of the foam-filled aneurysms reached at least 94% thrombus occlusion regardless of foam pore size or orientation, whereas the final thrombus occlusion within the coil-filled aneurysms varied from 80.8 to 92.2% with many of the cases leaving large areas in the aneurysm neck unfilled. Based on the simulations presented here, shape memory polymer foams may be able to produce more predictable, uniform, and complete clotting results than bare metal coils, independent of foam geometry or orientation.
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Aneurisma/complicaciones , Simulación por Computador , Polímeros/farmacología , Trombosis/complicaciones , Trombosis/prevención & control , Polímeros/químicaRESUMEN
Cardiovascular implantable devices alter the biofluid dynamics and biochemistry of the blood in which they are placed. These perturbations can lead to thrombus formation which may or may not be desired, depending on the application. In this work, a computational model is developed that couples biofluid dynamics and biochemistry to predict the clotting response of blood to such devices. The model consists of 28 advection-diffusion-reaction partial differential equations to track proteins in the blood involved in clotting and utilizes boundary flux terms to model the initiation of the intrinsic clotting pathway at thrombogenic device surfaces. We use this model to simulate the transient clot growth within a 2D idealized bifurcation aneurysm filled with various distributions of bare metal coils with similar packing densities. The clot model predicts initial clot formation to occur in areas along coil surfaces where flow is minimal and where time-averaged shear rates are the smallest. Among the six coil-filled aneurysm cases simulated, maximum thrombus occlusion ranged between 80.8 and 92.2% of the post-treatment aneurysm volume and was achieved 325-450 s after treatment. With further refinement and validation, the computational clotting model will be a valuable engineering tool for evaluating and comparing the relative performance of cardiovascular implantable devices.
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Procedimientos Endovasculares/instrumentación , Aneurisma Intracraneal/fisiopatología , Aneurisma Intracraneal/cirugía , Metales/química , Trombosis/fisiopatología , Algoritmos , Animales , Coagulación Sanguínea , Velocidad del Flujo Sanguíneo , Simulación por Computador , Difusión , Humanos , Hidrodinámica , Cinética , Modelos Cardiovasculares , Prótesis e Implantes , Reproducibilidad de los Resultados , TromboemboliaRESUMEN
Here we report the first example of a class of additively manufactured carbon fiber reinforced composite (AMCFRC) materials which have been achieved through the use of a latent thermal cured aromatic thermoset resin system, through an adaptation of direct ink writing (DIW) 3D-printing technology. We have developed a means of printing high performance thermoset carbon fiber composites, which allow the fiber component of a resin and carbon fiber fluid to be aligned in three dimensions via controlled micro-extrusion and subsequently cured into complex geometries. Characterization of our composite systems clearly show that we achieved a high order of fiber alignment within the composite microstructure, which in turn allows these materials to outperform equivalently filled randomly oriented carbon fiber and polymer composites. Furthermore, our AM carbon fiber composite systems exhibit highly orthotropic mechanical and electrical responses as a direct result of the alignment of carbon fiber bundles in the microscale which we predict will ultimately lead to the design of truly tailorable carbon fiber/polymer hybrid materials having locally programmable complex electrical, thermal and mechanical response.
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Predominantly closed-cell low density shape memory polymer (SMP) foam was recently reported to be an effective aneurysm filling device in a porcine model (Rodriguez et al., Journal of Biomedical Materials Research Part A 2013: (http://dx.doi.org/10.1002/jbm.a.34782)). Because healing involves blood clotting and cell migration throughout the foam volume, a more open-cell structure may further enhance the healing response. This research sought to develop a non-destructive reticulation process for this SMP foam to disrupt the membranes between pore cells. Non-destructive mechanical reticulation was achieved using a gravity-driven floating nitinol pin array coupled with vibratory agitation of the foam and supplemental chemical etching. Reticulation resulted in a reduced elastic modulus and increased permeability, but did not impede the shape memory behavior. Reticulated foams were capable of achieving rapid vascular occlusion in an in vivo porcine model.
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Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Vasos Sanguíneos/efectos de los fármacos , Ensayo de Materiales , Fenómenos Mecánicos , Polímeros/química , Polímeros/farmacología , Aleaciones/química , Animales , Vasos Sanguíneos/citología , Vasos Sanguíneos/fisiología , Membrana Celular/efectos de los fármacos , Hemostasis/efectos de los fármacos , Permeabilidad , PorcinosRESUMEN
Snakes demonstrate a great deal of variation in the amount of venom injected in both predatory and defensive strikes. There is some evidence that snakes can adaptively meter venom dosage. An underlying assumption in the evolution of venom metering is that the production of venom is energetically costly. To date there has been very little research that has quantified the metabolic costs associated with venom production. We used open-flow respirometry to test for significant differences between Prairie Rattlesnakes (Crotalus v. viridis) that had venom extracted and control snakes that did not. Although previous studies demonstrated high metabolic costs for venom production, we found that snakes that had venom extracted did not have significantly higher metabolic rates than control snakes. The pattern of metabolic deviation from baseline measurements was similar for both treatments, and on average snakes that had venom extracted only exhibited a 1.1% increase over baseline compared to a 2.5% increase in control snakes. Our data suggest that venom is not energetically costly to produce and that perhaps other costs associated with venom can better explain the variability in venom expenditure.
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Venenos de Crotálidos/biosíntesis , Crotalus/metabolismo , Metabolismo Energético , Animales , EspirometríaRESUMEN
BACKGROUND: Shape memory polymer (SMP) foams are being investigated as an alternative aneurysm treatment method to embolic coils. The goal of both techniques is the reduction of blood flow into the aneurysm and the subsequent formation of a stable thrombus, which prevents future aneurysm rupture. The purpose of this study is to experimentally determine the parameters, permeability and form factor, which are related to the flow resistance imposed by both media when subjected to a pressure gradient. METHODS: The porous media properties-permeability and form factor-of SMP foams and mock embolic coils (MECs) were measured with a pressure gradient method by means of an in vitro closed flow loop. We implemented the Forchheimer-Hazen-Dupuit-Darcy equation to calculate these properties. Mechanically-reticulated SMP foams were fabricated with average cell sizes of 0.7E-3 and 1.1E-3 m, while the MECs were arranged with volumetric packing densities of 11-28%. RESULTS: The permeability of the SMP foams was an order of magnitude lower than that of the MECs. The form factor differed by up to two orders of magnitude and was higher for the SMP foams in all cases. The maximum flow rate of all samples tested was within the inertial laminar flow regime, with Reynolds numbers ranging between 1 and 35. CONCLUSIONS: The SMP foams impose a greater resistance to fluid flow compared to MECs, which is a result of increased viscous and inertial losses. These results suggest that aneurysms treated with SMP foam will have flow conditions more favorable for blood stasis than those treated with embolic coils having packing densities ≤ 28%.
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Procedimientos Endovasculares/instrumentación , Polímeros , Hidrodinámica , Aneurisma Intracraneal/cirugía , Permeabilidad , PorosidadRESUMEN
A deployable, shape memory polymer adapter is investigated for reducing the hemodynamic stress caused by dialysis needle flow impingement within an arteriovenous graft. Computational fluid dynamics simulations of dialysis sessions with and without the adapter demonstrate that the adapter provides a significant decrease in the wall shear stress. Preliminary in vitro flow visualization measurements are made within a graft model following delivery and actuation of a prototype shape memory polymer adapter. Both the simulations and the qualitative flow visualization measurements demonstrate that the adapter reduces the severity of the dialysis needle flow impingement on the vascular access graft.
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Anastomosis Quirúrgica/instrumentación , Prótesis Vascular , Diálisis/instrumentación , Agujas , Polímeros/química , Materiales Biocompatibles/química , Presión Sanguínea , Diálisis/métodos , Elasticidad , Diseño de Equipo , Análisis de Falla de Equipo , Estrés MecánicoRESUMEN
The vascular dynamics of a shape memory polymer foam aneurysm treatment technique are assessed through the simulated treatment of a generic basilar aneurysm using coupled fluid dynamics and heat transfer calculations. The shape memory polymer foam, which expands to fill the aneurysm when heated, is modeled at three discrete stages of the treatment procedure. To estimate an upper bound for the maximum amount of thermal damage due to foam heating, a steady velocity is specified through the basilar artery, corresponding to a minimum physiological flow velocity over a cardiac cycle. During expansion, the foam alters the flow patterns within the aneurysm by shielding the aneurysm dome from a confined jet that issues from the basilar artery. The time scales for thermal damage to the artery walls and surrounding blood flow are computed from the temperature field. The flow through the post-treatment bifurcation is comprised of two counter-rotating vortex tubes that are located beneath the aneurysm neck and extend downstream into the outlet arteries. Beneath the aneurysm neck, a marked increase in the wall shear stress is observed due to the close proximity of the counter-rotating vortex tubes to the artery wall.
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Aneurisma Intracraneal/terapia , Polímeros/química , Polímeros/uso terapéutico , Arteria Basilar/fisiopatología , Velocidad del Flujo Sanguíneo , Simulación por Computador , Humanos , Aneurisma Intracraneal/fisiopatología , Modelos Cardiovasculares , Estrés Mecánico , TemperaturaRESUMEN
Conventional embolization of cerebral aneurysms using detachable coils is time-consuming and often requires retreatment. These drawbacks have prompted the development of new methods of aneurysm occlusion. We present the fabrication and laser deployment of a shape memory (SMP) polymer expanding foam device. Data acquired in an in vitro basilar aneurysm model with and without flow showed successful treatment, with the flow rate affecting foam expansion and the temperature at the aneurysm wall.