[Combination of pulmonary lymphangioleiomyomatosis with skin leiomyomatosis and uterine leiomyoma]. / Sochetanie limfangioleiomiomatoza legkikh, leiomiomatoza kozhi i leiomiomy matki.

Clinical observation with morphological verification of the above combination in a 48-year-old patient is presented. Large cysts, dystelectasia, fibrosis and smooth muscle proliferation were found in the lungs. The characteristic feature of smooth muscle cells was their differentiation in the direction of myofibroblasts and expression of antigen interacting with HMB-45 antibodies as well as protein products involved in catecholamine metabolism and estrogen receptors.

[Mutagenic properties of irradiated fresh fish in a chronic experiment]. / Izuchenie mutagennykh svoistv radurizovannoi svezhei ryby v khronicheskom éksperimente.

The mutagenic action of fresh fish irradiated in a dose of 200 kGy was studied in chronic experiments on 5 generations of rats. The mutagenic activity was assessed from the induction of dominant lethal mutations in sex cells of males and from the formation of chromosome aberrations (bridges and fragments) in bone marrow. The results of calculating the mutagenicity rate and cytogenetic analysis indicated no mutagenic action of irradiated fresh fish on sex cells and bone marrow of rats given irradiated fish for a long period of time.

[Macrophage migration inhibition test in mice with experimental amyloidosis]. / Reaktsiia tormozheniia migratsii makrofagov pri éksperimental'nom amiloidoze u myshei.

The migration inhibition factor (MIF) has been detected in sera of both amyloid patients and mice with casein-induced amyloidosis. This factor inhibits the migration of intact mouse splenocytes and peritoneal macrophages of the guinea pig. The same activity is also displayed by sera of guinea pig. The same activity is also displayed by sera of patients with chronic active hepatitis, liver cirrhosis and systemic lupus erythematosus. Spontaneous migration of splenocytes was studied in the course of casein-induced amyloidosis in mice. In the early stages of antigenic stimulation there was an increase in the cell migration activity, corresponding with the morphological picture of pyroninophilic and plasma cell proliferation. In the course of further antigenic stimulation the migration activity gradually decreases as the lymphoid tissue gets replaced by amyloid mass. MIF production during amyloidosis is discussed.

[Amyloid transfer from a syngenous transplant of amyloid spleen in intact and amyloid mice]. / Perenos amiloida iz singennogo transplantata amiloidnoi selezenki u intaktnykh i amiloidnykh myshei.

Investigations were carried out on male CBA mice with casein-induced amyloidosis. Fragments of spleens from the amyloid and intact donors were transplanted simultaneously to the opposite kidney poles, subcapsularly, to the intact and amyloid recipients. Amyloid deposits were found in 40 per cent of intact recipients both in their own spleens and in the grafts from intact donors. In amyloid recipients amyloid deposits developed in the grafts from intact donors only in 5 per cent of the animals (observation periods from 5 days to 6 months after the transplantation). It is amyloidogenic cells migration and the depression of this mechanism in amyloid mice.

[Cytogenetic activity of freshly irradiated potato]. / Izuchenie tsitogeneticheskoi aktivnosti svezheobluchennogo kartofelia.

The anaphasic method was employed in studying the frequency of chromosomal aberrations in the bone marrow cells of mice after feeding them on irradiated and non-irradiated potatoes. The latter were irradiated raw with gamma-rays in a dose of 10 krad and then fed to the animals for 5 days 24 hours upon irradiation in an amount of 3 g per animal to male albino mongrel mice (10 in each group), viz. in the 1st test series--raw and in the II--cooked. As controls in both series served raw or cooked non-irradiated potatoes. A total of 12 800 cells from 40 animals were counted for the presence of bridges and fragments. The results of the cytogenetic analysis bore proof to the absence of any statistically significant difference in the frequence of chromosomal aberrations in the bone marrow cells of mice fed on freshly-irradiated potatoes and in those of control animals.

[Influence of the storage and culinary processing of irradiated potatoes on the cytogenetic activity of extracts obtained from them]. / Vliianie khraneniia i kulinarnoi obrabotki obluchennogo kartofelia na tsitogeneticheskuiu aktivnost' vydelennykh iz nego ékstraktov

Male-rats (25--27 g) were given perorally extracts separated from potato subjected to gamma-radiation in a dose of 10 krads (test groups) and from nonirradiated tubers (controls). The extracts were introduced for a period of one week, daily in an amount of 1 ml. The male from the test groups (each numbering 8--10 animals) received extracts of the raw potato stored for 4 months after irradiation and of the potato subjected to thermal treatment (cooking) after 1 day, 1 and 4 months of its storage. The frequency of chromosomal aberrations in the bone marrow cells was determined by the anaphase method. Altogether about 34 thous. cells (500-600 from each animal) were counted. The results testified to a significantly reduced frequency of chromosomal aberrations (bridges and fragments) occurring in the bone marrow cells of the mice which received extracts from the raw stored irradiated potato and from thermally treated freshly irradiated tubers, as compared to extracts obtained from the raw freshly irradiated potatoes. The extracts of irradiated potato cooked after 1 and 4 month of storage did not display any mutagenic properties.

Toxicologic and hygienic investigation of potatoes irradiated with a beam of fast electrons and gamma-rays to control sprouting.

Potatoes exposed either to gamma-rays at a dose of 20 krad, or irradiated with a beam of fast electrons at a dose of 30 krad to inhibit the sprouting of tubers, were studied for mutagenic effects. Toxic action and influence on the fertility were also investigated. Extracts of irradiated potatoes (10 krad gamma-rays), stored and then boiled, were also studied for their mutagenic effects. The induction of dominant lethal mutations (DLM) was used as indicator of mutagenic action of potatoes in male rats, that of the extracts of potatoes in male mice. Experimental animals (white rats) were fed with irradiated potatoes. Neither a toxic, nor a mutagenic action was found. Oral treatment of male mice with extracts of irradiated potatoes also did not increase the frequency of DLM in male mice.