Enhancing BIM implementation in Spanish public procurement: A framework approach.

The European Union supports the use of technology to improve public procurement, acknowledging Building Information Modeling (BIM) as a catalyst for cost-effective public works and innovation. The purpose of this paper is to evaluate BIM implementation practices in Spanish public procurement. The methodology used in this study is a mixed research method involving a questionnaire survey and semi-structured expert interviews. The findings reveal market maturity as a barrier to BIM adoption in Spanish public procurement throughout the asset lifecycle. Selecting the right instrument for implementation proves challenging for public organizations. This study has contributed to the development of more practical and effective strategies to ensure full adoption of BIM within the public procurement sector of Spain. It proposes a framework approach for the pre-contractual phase, helping contracting authorities to make the optimal instrument selection. Two criteria are considered: the maturity of the public client and sector, and the economic value classification of tenders.

"MiR-7 controls cholesterol biosynthesis through posttranscriptional regulation of DHCR24 expression".

Dysregulation of cholesterol homeostasis is associated with several pathologies including cardiovascular diseases and neurological disorders such as Alzheimer's disease (AD). MicroRNAs (miRNAs) have emerged as key post-transcriptional regulators of cholesterol metabolism. We previously established the role of miR-7 in regulating insulin resistance and amyloidosis, which represents a common pathological feature between type 2 diabetes and AD. We show here an additional metabolic function of miR-7 in cholesterol biosynthesis. We found that miR-7 blocks the last steps of the cholesterol biosynthetic pathway in vitro by targeting relevant genes including DHCR24 and SC5D posttranscriptionally. Intracranial infusion of miR-7 on an adeno-associated viral vector reduced the expression of DHCR24 in the brain of wild-type mice, supporting in vivo miR-7 targeting. We also found that cholesterol regulates endogenous levels of miR-7 in vitro, correlating with transcriptional regulation through SREBP2 binding to its promoter region. In parallel to SREBP2 inhibition, the levels of miR-7 and hnRNPK (the host gene of miR-7) were concomitantly reduced in brain in a mouse model of Niemann Pick type C1 disease and in murine fatty liver, which are both characterized by intracellular cholesterol accumulation. Taken together, the results establish a novel regulatory feedback loop by which miR-7 modulates cholesterol homeostasis at the posttranscriptional level, an effect that could be exploited for therapeutic interventions against prevalent human diseases.

Eficacia de los tratamientos psicológicos para los comportamientos autolesivos suicidas y no suicidas en adolescentes / Efficacy of psychological treatments for suicidal and non-suicidal self-injurious behaviors in adolescents

Los comportamientos autolesivos y suicidas en adolescentes, por su creciente prevalencia como por sus fatales consecuencias, son en nuestrosdías un problema de salud pública que requiere de intervenciones eficaces. Con el objetivo de dilucidar los tratamientos psicológicos que, hasta lafecha, mejor funcionan para reducir o eliminar estos comportamientos, se realizó una revisión de estudios de metaanálisis y revisiones sistemáticasen las bases de datos PsycArticles, PsycInfo y MEDLINE. Los criterios de elegibilidad establecidos permitieron la selección final de 10 registros.Los resultados mostraron una elevada heterogeneidad y un alcance limitado de los tratamientos psicológicos con tamaños del efecto de pequeñosa moderados tanto para las conductas autolesivas, como para la ideación suicida, la suicidabilidad y especialmente para la intención suicida. Estosefectos tendían a debilitarse o desaparecer en el medio y largo plazo. La Terapia Dialéctica Conductual (TDC) resultó ser comparativamente la quemejores resultados obtuvo de las intervenciones analizadas. El alcance restringido de los resultados y las importantes limitaciones metodológicashalladas alertan de la necesidad de más investigación, así como del futuro diseño de intervenciones específicas para este tipo de problemas. (AU)

Self-injurious and suicidal behaviors in adolescents are nowadays a public health problem that requires effective interventions. In order to elucidate the psychological treatments that, to date,work best to reduce or eliminate these behaviors, a review of meta-analysis and systematic reviews was carried out in the PsycArticles, PsycInfo andMEDLINE databases. The established eligibility criteria allowed the final selection of 10 records. The results showed high heterogeneity and poorresults for the psychological treatments with small to moderate effect sizes for self-injurious behaviors, suicidal ideation, suicidality and especiallyfor suicidal attempts. These effects tended to weaken or disappear in the medium to long term. Dialectical Behavior Therapy (DBT) proved to becomparatively the best performing of the interventions analyzed. The limited scope of the results and the important methodological limitations alertus to the need for further research and the future design of specific interventions for this type of problems. (AU)

Role of miR-199a-5p in the post-transcriptional regulation of ABCA1 in response to hypoxia in peritoneal macrophages.

Hypoxia is a crucial factor contributing to maintenance of atherosclerotic lesions. The ability of ABCA1 to stimulate the efflux of cholesterol from cells in the periphery, particularly foam cells in atherosclerotic plaques, is an important anti-atherosclerotic mechanism. The posttranscriptional regulation by miRNAs represents a key regulatory mechanism of a number of signaling pathways involved in atherosclerosis. Previously, miR-199a-5p has been shown to be implicated in the endocytic and retrograde intracellular transport. Although the regulation of miR-199a-5p and ABCA1 by hypoxia has been already reported independently, the role of miR-199a-5p in macrophages and its possible role in atherogenic processes such us regulation of lipid homeostasis through ABCA1 has not been yet investigated. Here, we demonstrate that both ABCA1 and miR-199a-5p show an inverse regulation by hypoxia and Ac-LDL in primary macrophages. Moreover, we demonstrated that miR-199a-5p regulates ABCA1 mRNA and protein levels by directly binding to its 3'UTR. As a result, manipulation of cellular miR-199a-5p levels alters ABCA1 expression and cholesterol efflux in primary mouse macrophages. Taken together, these results indicate that the correlation between ABCA1-miR-199a-5p could be exploited to control macrophage cholesterol efflux during the onset of atherosclerosis, where cholesterol alterations and hypoxia play a pathogenic role.

New Insights on the Regulation of the Insulin-Degrading Enzyme: Role of microRNAs and RBPs.

The evident implication of the insulin-degrading enzyme (IDE) in Alzheimer's disease (AD) and type 2 diabetes mellitus (T2DM), among its capacity to degrade insulin and amyloid-ß peptide (Aß), suggests that IDE could be an essential link in the relation between hyperinsulinemia, insulin resistance and AD. However, little is known about the cellular and molecular regulation of IDE expression, and even less has been explored regarding the post-transcriptional regulation of IDE, although it represents a great molecular target of interest for therapeutic treatments. We recently described that miR-7, a novel candidate for linking AD and T2DM at the molecular level, regulates IDE and other key genes in both pathologies, including some key genes involved in the insulin signaling pathway. Here, we explored whether other miRNAs as well as other post-transcriptional regulators, such as RNA binding proteins (RBP), could potentially participate in the regulation of IDE expression in vitro. Our data showed that in addition to miR-7, miR-125, miR-490 and miR-199 regulate IDE expression at the post-transcriptional level. Moreover, we also found that IDE contains multiple potential binding sites for several RBPs, and a narrow-down prediction analysis led us to speculate on a novel regulation of IDE by RALY and HuD. Taken together, these results demonstrate the novel players controlling IDE expression that could represent potential therapeutical targets to treat several metabolic diseases with a high impact on human health, including AD and T2DM.

Functional Impairment in Borderline Personality Disorder: The Mediating Role of Perceived Social Support.

Borderline personality disorder (BPD) is characterized by instability in relationships, mood fluctuations, and erratic behavior. This study investigates the relationship between pathological personality traits and functional disability, the status of perceived social support in BPD, as well as its mediating role in this relationship. In this cross-sectional study, 192 Spanish women (BPD group, N = 97; healthy control group, N = 95) completed, through two online platforms, a battery of tests including: the Personality Inventory for DSM-5 Brief Form (PID-5-BF), the World Health Organization Disability Assessment Schedule 2.0 (WHODAS 2.0) and the Perceived Social Support subscale of the Quality of Life Questionnaire (QLQ). The results show that perceived social support was significantly lower in the BPD group, which also presented a significantly higher disability score than the control group. Pathological personality traits affected functionality both directly and indirectly through perceived social support, as this variable was a significant mediator in both groups. We conclude that perceived social support is impaired in BPD patients, and enhancing it as a complementary therapy to evidence-based treatments could help preserve the functionality of patients while pathological traits are regulated. This study also encourages future research to delve into the relevance of other psychosocial variables on the functionality of subjects with BPD, and the need of enhancing them in therapy.

A Common Variant at the 3'untranslated Region of the CCL7 Gene (rs17735770) Is Associated With Decreased Susceptibility to Coronary Heart Disease.

Monocytes participate in the development of atherosclerosis through the action of cytokines and other inflammatory mediators. Among them, CCR2 and its ligands, CCL2 and CCL7 play an important role, so the main objective of this work was to determine whether genetic variants affecting their activity were associated with cardiovascular disease. A cohort of 519 patients that have suffered coronary events was analyzed under a propensity score-matching protocol selecting a homogeneous set of cases and controls, according to age, sex, smoking status, dyslipidemia, arterial hypertension and type 2 diabetes as risk factors. While dyslipidemia and arterial hypertension were more prevalent among patients with angina pectoris, current smoking status and elevated inflammatory markers, including total leukocyte and monocyte counts, were more likely associated with acute coronary events. Propensity score matching analysis, performed to eliminate the influence of these risk factors and highlight genetic modifiers, revealed that a single nucleotide variant, rs17735770 at the 3'untranslated region of the CCL7 gene transcript, was associated with decreased cardiovascular risk in a group represented mostly by men, with an average age of 57, and without significant differences in traditional risk factors. Furthermore, the presence of this variant altered the local mRNA structure encompassing a binding site for miR-23ab, resulting in increased translation of a reporter gene in a miR23 independent fashion. The rs17735770 genetic variant led to increased expression of CCL7, a potential antagonist of CCR2 at inflammatory sites, where it could play a meaningful role during the evolution of atherosclerosis.

La intolerancia a la incertidumbre, la tendencia a experimentar preocupaciones y el pesimismo como factores intervinientes en la relación entre la dependencia emocional y la violencia / Intolerance to uncertainty, the tendency to experience worry and pessimism as factors in the relationship between emotional dependence and violence

Las personas con dependencia emocional presentan preocupaciones constantes sobre el posible abandono o traición por parte de la pareja. De esta forma, ponen en marcha un gran abanico de estrategias retentivas dirigidas a cubrir la gran necesidad de controlar la relación. Además, sienten insatisfacción y frustración en la relación ya que sienten que nunca conseguirán lo que quieren o esperan de ella. Por esto, los objetivos del estudio se dirigen a analizar la posible relación entre la dependencia emocional, la intolerancia a la incertidumbre, la tendencia a las preocupaciones y el pesimismo. Asimismo, se comprueba el papel mediador de estos factores en la relación entre la dependencia emocional y la permanencia en relaciones de pareja violentas debido a la elevada prevalencia de darse conjuntamente en una misma relación. Participaron 258 personas, 77.1% mujeres y 22.9% hombres con edades que oscilaron entre los 18 y 67 años (M = 32.63; DT = 11.66). Los resultados reflejaron relaciones positivas entre la dependencia emocional, la intolerancia a la incertidumbre, la tendencia a las preocupaciones y el pesimismo. Igualmente, se comprueba que la dependencia emocional conduce a la permanencia en relaciones violentas a través de los factores mencionados.(AU)

People with emotional dependence present constant concerns about possible abandonment or betrayal by their partner. In this way, they implement a wide range of retention strategies aimed at covering the great need for control in the relationship. In addition, they feel dissatisfaction and frustration in the relationship since they feel they will never get what they want or expect from it. Therefore, the objectives of the study are aimed at analyzing the possible relationship between emotional dependence, intolerance to uncertainty, tendency to worry and pessimism. Also, the mediating role of these factors in the relationship between emotional dependence and permanence in violent relationships is verified due to the high prevalence of occurring jointly in the same relationship. A total of 258 people participated, 77.1% women and 22.9% men with ages ranging from 18 to 67 (M= 32.63; SD= 11.66). The results reflected positive relationships between emotional dependence, intolerance to uncertainty, tendency to worry andpessimism. Likewise, it is proven that emotional dependence leads to permanence in violent relationships through the factors mentioned.(AU)

La dependencia emocional hacia la pareja agresora y su relación con la ansiedad social, el miedo a la evaluación negativa y el perfeccionismo disfuncional / [Emotional dependence on the aggressor partner and its relationship with social anxiety, fear of negative evaluation and dysfunctional perfectionism]

Los objetivos del estudio fueron evaluar la relación entre la dependencia emocional y la ansiedad social, el miedo a la evaluación negativa y el perfeccionismo disfuncional, así como su papel mediador en la relación entre las influencias paternas perfeccionistas y la violencia física recibida por parte de la pareja. Participaron 258 personas (77,1% mujeres) de entre 18 y 67 años (M= 32,63; DT= 11,66). Los hallazgos sugirieron que la dependencia emocional estaba positivamente asociada a la violencia de pareja recibida, ansiedad social, miedo a la evaluación negativa y perfeccionismo disfuncional. A su vez, la violencia de pareja recibida estaba positivamente asociada al perfeccionismo disfuncional. La dependencia emocional explicó el 16,55% de la relación entre las influencias paternas perfeccionistas, referidas a la percepción de las figuras parentales abiertamente críticas y con expectativas elevadas y la permanencia en relaciones de pareja violentas físicamente. En conclusión, se menciona la necesidad de investigar e incluir estos factores en las intervenciones psicológicas dirigidas a tratar ambas problemáticas como son la dependencia emocional y la permanencia en relaciones violentas.

The objectives of the study were to evaluate the relationship between emotional dependence and social anxiety, fear of negative evaluation and dysfunctional perfectionism, as well as its mediating role in the relationship between perfectionistic parental influences and physical violence received from the partner. A total of 258 people (77.1% female) aged 18-67 years (M= 32.63, SD= 11.66) participated. The findings suggested that emotional dependence was positively associated with intimate partner violence received, social anxiety, fear of negative evaluation, and dysfunctional perfectionism. In turn, intimate partner violence received was positively associated with dysfunctional perfectionism. Emotional dependence explained 16.55% of the relationship between perfectionist parental influences, referring to the perception of openly critical parental figures with high expectations, and the permanence in physically violent intimate partner relationships. In conclusion, the need to investigate and include these factors in psychological interventions aimed at treating both problems such as emotional dependence and permanence in violent relationships is mentioned.

Posttranscriptional Regulation of Insulin Resistance: Implications for Metabolic Diseases.

Insulin resistance defines an impairment in the biologic response to insulin action in target tissues, primarily the liver, muscle, adipose tissue, and brain. Insulin resistance affects physiology in many ways, causing hyperglycemia, hypertension, dyslipidemia, visceral adiposity, hyperinsulinemia, elevated inflammatory markers, and endothelial dysfunction, and its persistence leads to the development metabolic disease, including diabetes, obesity, cardiovascular disease, or nonalcoholic fatty liver disease (NAFLD), as well as neurological disorders such as Alzheimer's disease. In addition to classical transcriptional factors, posttranscriptional control of gene expression exerted by microRNAs and RNA-binding proteins constitutes a new level of regulation with important implications in metabolic homeostasis. In this review, we describe miRNAs and RBPs that control key genes involved in the insulin signaling pathway and related regulatory networks, and their impact on human metabolic diseases at the molecular level, as well as their potential use for diagnosis and future therapeutics.

Preventing Oxidative Stress in the Liver: An Opportunity for GLP-1 and/or PASK.

The liver's high metabolic activity and detoxification functions generate reactive oxygen species, mainly through oxidative phosphorylation in the mitochondria of hepatocytes. In contrast, it also has a potent antioxidant mechanism for counterbalancing the oxidant's effect and relieving oxidative stress. PAS kinase (PASK) is a serine/threonine kinase containing an N-terminal Per-Arnt-Sim (PAS) domain, able to detect redox state. During fasting/feeding changes, PASK regulates the expression and activation of critical liver proteins involved in carbohydrate and lipid metabolism and mitochondrial biogenesis. Interestingly, the functional inactivation of PASK prevents the development of a high-fat diet (HFD)-induced obesity and diabetes. In addition, PASK deficiency alters the activity of other nutrient sensors, such as the AMP-activated protein kinase (AMPK) and the mammalian target of rapamycin (mTOR). In addition to the expression and subcellular localization of nicotinamide-dependent histone deacetylases (SIRTs). This review focuses on the relationship between oxidative stress, PASK, and other nutrient sensors, updating the limited knowledge on the role of PASK in the antioxidant response. We also comment on glucagon-like peptide 1 (GLP-1) and its collaboration with PASK in preventing the damage associated with hepatic oxidative stress. The current knowledge would suggest that PASK inhibition and/or exendin-4 treatment, especially under fasting conditions, could ameliorate disorders associated with excess oxidative stress.

Storage and Utilization of Glycogen by Mouse Liver during Adaptation to Nutritional Changes Are GLP-1 and PASK Dependent.

Glucagon-like peptide 1 (GLP-1) and PAS kinase (PASK) control glucose and energy homeostasis according to nutritional status. Thus, both glucose availability and GLP-1 lead to hepatic glycogen synthesis or degradation. We used a murine model to discover whether PASK mediates the effect of exendin-4 (GLP-1 analogue) in the adaptation of hepatic glycogen metabolism to nutritional status. The results indicate that both exendin-4 and fasting block the Pask expression, and PASK deficiency disrupts the physiological levels of blood GLP1 and the expression of hepatic GLP1 receptors after fasting. Under a non-fasted state, exendin-4 treatment blocks AKT activation, whereby Glucokinase and Sterol Regulatory Element-Binding Protein-1c (Srebp1c) expressions were inhibited. Furthermore, the expression of certain lipogenic genes was impaired, while increasing Glucose Transporter 2 (GLUT2) and Glycogen Synthase (GYS). Moreover, exendin-4 treatment under fasted conditions avoided Glucose 6-Phosphatase (G6pase) expression, while maintaining high GYS and its activation state. These results lead to an abnormal glycogen accumulation in the liver under fasting, both in PASK-deficient mice and in exendin-4 treated wild-type mice. In short, exendin-4 and PASK both regulate glucose transport and glycogen storage, and some of the exendin-4 effects could therefore be due to the blocking of the Pask expression.

Crosstalk Between LXR and Caveolin-1 Signaling Supports Cholesterol Efflux and Anti-Inflammatory Pathways in Macrophages.

Macrophages are immune cells that play crucial roles in host defense against pathogens by triggering their exceptional phagocytic and inflammatory functions. Macrophages that reside in healthy tissues also accomplish important tasks to preserve organ homeostasis, including lipid uptake/efflux or apoptotic-cell clearance. Both homeostatic and inflammatory functions of macrophages require the precise stability of lipid-rich microdomains located at the cell membrane for the initiation of downstream signaling cascades. Caveolin-1 (Cav-1) is the main protein responsible for the biogenesis of caveolae and plays an important role in vascular inflammation and atherosclerosis. The Liver X receptors (LXRs) are key transcription factors for cholesterol efflux and inflammatory gene responses in macrophages. Although the role of Cav-1 in cellular cholesterol homeostasis and vascular inflammation has been reported, the connection between LXR transcriptional activity and Cav-1 expression and function in macrophages has not been investigated. Here, using gain and loss of function approaches, we demonstrate that LXR-dependent transcriptional pathways modulate Cav-1 expression and compartmentation within the membrane during macrophage activation. As a result, Cav-1 participates in LXR-dependent cholesterol efflux and the control of inflammatory responses. Together, our data show modulation of the LXR-Cav-1 axis could be exploited to control exacerbated inflammation and cholesterol overload in the macrophage during the pathogenesis of lipid and immune disorders, such as atherosclerosis.

El consumo de sustancias y su relación con la dependencia emocional, el apego y la regulación emocional en adolescentes / Substance abuse and its relationship to emotional dependence, attachment and emotional regulation in adolescents

El consumo de sustancias entre los adolescentes supone un grave problema social que podría relacionarse con la dependencia emocional, estilos de apego y dificultades en la regulación emocional. De esta forma, los objetivos del presente estudio fueron analizar la relación y el papel predictivo de la dependencia emocional, apego y regulación emocional sobre el consumo de sustancias el último mes. Además, se analizaron las diferencias en función del género y edad en todas las variables del estudio. Igualmente, se comprobó el papel mediador de la regulación emocional y los estilos de apego en la relación entre la dependencia emocional y el consumo de sustancias. La muestra empleada estaba compuesta por 1.533 adolescentes escolarizados, 826 hombres y 707 mujeres con edades entre los 13 y los 22 años (M = 15.76, DT = 1.25). Los resultados mostraron una relación positiva entre el consumo de sustancias y la dependencia emocional. Del mismo modo, las personas consumidoras de sustancias manifestaron relaciones positivas con las dificultades de regulación emocional, así como, con los estilos de apego de permisividad parental, autosuficiencia y rencor contra los padres y traumatismo infantil y relaciones negativas con la seguridad, preocupación familiar, interferencia parental y valor a la autoridad parental

Substance use among adolescents is a serious social problem that may be related to emotional dependence, attachment styles and diffi-culties in emotional regulation. Thus, the objectives of this study were to analyse the relationship and predictive role of emotional dependence, at-tachment and emotional regulation on substance use in the last month. In addition, gender and age differences were analysed in all variables of the study. The mediating role of emotional regulation and attachment styles in the relationship between emotional dependence and substance use was al-so verified. The sample used was made up of 1.533 schooled adolescents, 826 men and 707 women with ages ranging from 13 to 22 years (M = 15.76, SD = 1.25). The results showed a positive relationship between sub-stance use and emotional dependence. Similarly, substance users showed positive relationships with the difficulties of emotional regulation, as well as, with the attachment styles of parental permissiveness, self-sufficiency and resentment against parents and child trauma, and negative relation-ships with secure attachment, family concern, parental interference and value to parental authority

Estilos de afrontamiento, esquemas disfuncionales y síntomas psicopatológicos relacionados con la dependencia emocional hacia la pareja agresora / Coping styles, dysfunctional schemes and psychopathological symptoms related to emotional dependence on the aggressor partner

Los estilos de afrontamiento, los esquemas disfuncionales y la sintomatología psicopatológica podrían resultar factores de vulnerabilidad que incrementan la probabilidad de que una persona desarrolle dependencia emocional hacia la pareja agresora. Delimitar los factores de riesgo es fundamental para su prevención y tratamiento. Consecuentemente, el presente estudio tuvo como objetivos principales analizar la relación entre los factores señalados, así como el papel mediador de los tres primeros en la relación entre la dependencia emocional y la violencia recibida. La muestra estuvo conformada por 657 mujeres procedentes de población clínica y general, con edades que oscilaron entre los 18 y 66 años (M= 23,38; DT= 8,24). Los resultados reflejaron la predominancia del empleo de estilos de afrontamiento inadecuados, tales como pensamiento desiderativo, aislamiento social y autocrítica, síntomas psicopatológicos de depresión, ansiedad, sensibilidad interpersonal, obsesión compulsión e ideación paranoide, así como esquemas de abandono y subyugación. Asimismo, estos factores explicaron una parte de la relación entre la dependencia emocional y la permanencia en relaciones violentas

Coping styles, dysfunctional schemes and psychopathological symptoms could be factors of vulnerability that increase the probability that a person develops emotional dependence on an aggressive partner. Delimiting the risk factors is fundamental for its prevention and treatment. Consequently, the main objectives of the present study were to analyze the relationship between the above-mentioned factors, as well as the mediating role of the first three in the relationship between emotional dependence and received violence. The sample was made up of 657 women from the clinical and general population, with ages ranging from 18 to 66 years of age (M= 23.38, SD= 8.24). The results reflected the predominance of inadequate coping styles, such as desiderative thinking, Estudio financiado anxiety, interpersonal sensibility, obsession-compulsion and paranoid ideation, as well as abandonment and subjugation schemes. These factors also explained part of the relationship between emotional dependence and staying in violent relationships

La enfermería de quirófano ante la Hipertermia Maligna. ¿Estamos preparados? / Surgical Nurse on Malignant Hyperthermia. Are we prepared?

OBJETIVO PRINCIPAL: Evaluar el conocimiento de la Hipertermia Maligna (HM) entre los profesionales de Enfermería de quirófano. METODOLOGÍA: Estudio descriptivo transversal con 169 profesionales de Enfermería de quirófano. RESULTADOS PRINCIPALES: La puntuación total de los cuestionarios alcanzó un promedio de 4,8 (±2,18) puntos sobre 10 totales. Disponer de un protocolo para la asistencia de episodios de HM, se relacionó de forma significativa con un conocimiento adecuado. CONCLUSIÓN PRINCIPAL: Los profesionales de Enfermería de quirófano presentan unos inadecuados niveles de conocimiento sobre la HM y su manejo. Es necesaria una mejora en la preparación de las enfermeras para atender a un paciente en crisis de HM

MAIN OBJECTIVE: Assessing knowledge levels of the surgical nurses regarding Malignant Hyperthermia (MH) and its management. METHODOLOGY: Cross-sectional descriptive study among 169 surgical nurses. MAIN RESULTS: The total score of the questionnaires reached an average of 4.8 (± 2.18) points out of 10 total. Having an standart operating procedute for the assistance of MH episodes was significantly related to adequate knowledge. MAIN CONCLUSION: Surgical nurses have inadequate levels on managing MH and delivering inmediate care. It is necessary to provide specific trainning on MH for surgical nurses to prepare them to safely assist patients suffering MH crisis

PAS kinase deficiency reduces aging effects in mice.

Several signaling pathways may be affected during aging. All are regulated by nutrient levels leading to a decline in mitochondrial function and autophagy and to an increase in oxidative stress. PAS Domain Kinase (PASK) is a nutrient and bioenergetic sensor. We have previously found that PASK plays a role in the control of hepatic metabolic balance and mitochondrial homeostasis. To investigate PASK's role in hepatic oxidative stress during aging, we analyzed the mitochondrial function, glucose tolerance, insulin resistance, and lipid-related parameters in aged PASK-deficient mice. Hepatic Pask mRNA decreased in step with aging, being undetectable in aged wild-type (WT) mice. Aged PASK-deficient mice recorded lower levels of ROS/RNS compared to aged WT. The regulators of mitochondrial biogenesis, PGC1a, SIRT1 and NRF2, decreased in aged WT, while aged PASK-deficient mice recorded a higher expression of NRF2, GCLm and HO1 proteins and CS activity under fasted conditions. Additionally, aged PASK-deficient mice recorded an overexpression of the longevity gene FoxO3a, and maintained elevated PCNA protein, suggesting that hepatic cell repair mechanisms might be functional. PASK-deficient mice have better insulin sensitivity and no glucose intolerance, as confirmed by a normal HOMA-IR index. PASK may be a good target for reducing damage during aging.

PAS Kinase: A Nutrient and Energy Sensor "Master Key" in the Response to Fasting/Feeding Conditions.

The protein kinase with PAS domains (PASK) is a nutrient and energy sensor located in the cells of multiple organs. Many of the recent findings for understanding PASK functions in mammals have been reported in studies involving PASK-deficient mice. This minireview summarizes the PASK role in the control of fasting and feeding responses, focusing especially on the hypothalamus and liver. In 2013, PASK was identified in the hypothalamic areas involved in feeding behavior, and its expression was regulated under fasting/refeeding conditions. Furthermore, it plays a role in coordinating the activation/inactivation of the hypothalamic energy sensors AMPK and mTOR/S6K1 pathways in response to fasting. On the other hand, PASK deficiency prevents the development of obesity and non-alcoholic fatty liver in mice fed with a high-fat diet. This protection is explained by the re-establishment of several high-fat diet metabolic alterations produced in the expression of hepatic transcription factors and key enzymes that control the main metabolic pathways involved in maintaining metabolic homeostasis in fasting/feeding responses. This minireview covers the effects of PASK inactivation in the expression of certain transcription factors and target enzymes in several metabolic pathways under situations such as fasting and feeding with either a standard or a high-fat diet.

La dependencia emocional hacia la pareja agresora y su relación con los trastornos de la conducta alimentaria / Emotional dependence on the aggressive partner and its relationship to eating Disorders

Los trastornos de la conducta alimentaria (TCA) son frecuentes en las familias con dificultades para fomentar la autonomía de sus miembros, pudiendo resultar un factor de riesgo a la hora de establecer relaciones de pareja dependientes. La dependencia emocional y la violencia de pareja se dan conjuntamente con elevada frecuencia. El objetivo del estudio fue examinar la relación y el papel predictivo de los TCA en la dependencia emocional, violencia recibida y dependencia emocional hacia la pareja agresora. Participaron 712 personas, 545 mujeres y 167 hombres, de entre 18 y 30 años (M= 21,32; DT= 2,94). Se encontró que las personas que puntuaban alto en TCA eran un grupo de riesgo en el desarrollo de la dependencia emocional, permanencia en relaciones violentas y desarrollo de ambas conjuntamente en una misma relación de pareja. En cuanto a las características asociadas con los TCA, la impulsividad se asoció con la violencia recibida y el miedo a la madurez, ineficacia, perfeccionismo, ascetismo e impulsividad se asociaron con la dependencia emocional en general y hacia la pareja agresora

Eating disorders are common in families with difficulties in promoting the autonomy of their members and may be a risk factor when establishing dependent relationships. Emotional dependence and partner violence occur together with a high frequency. The aim of the study was to examine the relationship and predictive role of eating disorders in emotional dependence, violence received and emotional dependence on the aggressor partner. The participants were 712 subjects, 545 women and 167 men, between 18 and 30 years of age (M= 21.32, SD= 2.94). It was found that individuals who scored high on eating disorders were a risk group in the development of emotional dependence, permanence in violent relationships, and the development of both together in the same relationship. As for the characteristics associated with eating disorders, impulsivity was associated with the violence received and fear of maturity, ineffectiveness, perfectionism, asceticism and impulsivity were associated with emotional dependence in general and owards the aggressor partner

MicroRNA 7 Impairs Insulin Signaling and Regulates Aß Levels through Posttranscriptional Regulation of the Insulin Receptor Substrate 2, Insulin Receptor, Insulin-Degrading Enzyme, and Liver X Receptor Pathway.

Brain insulin resistance is a key pathological feature contributing to obesity, diabetes, and neurodegenerative disorders, including Alzheimer's disease (AD). Besides the classic transcriptional mechanism mediated by hormones, posttranscriptional regulation has recently been shown to regulate a number of signaling pathways that could lead to metabolic diseases. Here, we show that microRNA 7 (miR-7), an abundant microRNA in the brain, targets insulin receptor (INSR), insulin receptor substrate 2 (IRS-2), and insulin-degrading enzyme (IDE), key regulators of insulin homeostatic functions in the central nervous system (CNS) and the pathology of AD. In this study, we found that insulin and liver X receptor (LXR) activators promote the expression of the intronic miR-7-1 in vitro and in vivo, along with its host heterogeneous nuclear ribonucleoprotein K (HNRNPK) gene, encoding an RNA binding protein (RBP) that is involved in insulin action at the posttranscriptional level. Our data show that miR-7 expression is altered in the brains of diet-induced obese mice. Moreover, we found that the levels of miR-7 are also elevated in brains of AD patients; this inversely correlates with the expression of its target genes IRS-2 and IDE. Furthermore, overexpression of miR-7 increased the levels of extracellular Aß in neuronal cells and impaired the clearance of extracellular Aß by microglial cells. Taken together, these results represent a novel branch of insulin action through the HNRNPK-miR-7 axis and highlight the possible implication of these posttranscriptional regulators in a range of diseases underlying metabolic dysregulation in the brain, from diabetes to Alzheimer's disease.