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1.
Environ Toxicol Pharmacol ; 90: 103813, 2022 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-35033683

RESUMEN

Phthalates are endocrine disrupting compounds that have been found in outdoor and indoor air. However, little is known about their inhalatory absorption. Although measurement of urinary metabolites is the current standard, complex and convergent metabolism of phthalates poses the necessity for alternative methodologies such as the quantitation of parental compounds in plasma. We determined the inhalatory absorption of Diisobutyl phthalate (DiBP) using a novel method based on a thermal desorption probe (TSP)-gas chromatography-mass spectrometry developed for the detection and quantitation of nine phthalate diesters in blood plasma, which fulfilled the acceptance criteria suggested by FDA guidelines regarding specificity, matrix effect, recovery, linearity, sensitivity, accuracy, and precision. After inhalation, plasma concentration of DiBP exhibited two peaks, suggesting a first, rapid absorption event, followed by a second, delayed one and a first order elimination stage. Half-life was calculated as 62 min and bioavailability, compared to IV route, was 15%.


Asunto(s)
Dibutil Ftalato/análogos & derivados , Exposición por Inhalación/análisis , Ácidos Ftálicos/análisis , Animales , Análisis Químico de la Sangre/métodos , Dibutil Ftalato/toxicidad , Cromatografía de Gases y Espectrometría de Masas , Masculino , Ratas , Ratas Wistar , Toxicocinética
2.
J Helminthol ; 93(5): 539-547, 2019 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-30149822

RESUMEN

Androgens have been shown to exert a cysticidal effect upon Taenia crassiceps, an experimental model of cysticercosis. To further inquire into this matter, the Taenia crassiceps model was used to evaluate the expression of several proteins after testosterone (T4) and dihydrotestosterone (DHT) in vitro treatment. Under 2-D proteomic maps, parasite extracts were resolved into approximately 130 proteins distributed in a molecular weight range of 10-250 kDa and isoelectrical point range of 3-10. The resultant proteomic pattern was analysed, and significant changes were observed in response to T4 and DHT. Based on our experience with electrophoretic patterns and proteomic maps of cytoskeletal proteins, alteration in the expression of isoforms of actin, tubulin and paramyosin and of other proteins was assessed. Considering that androgens may exert their biological activity in taeniids through the non-specific progesterone receptor membrane component (PGRMC), we harnessed bioinformatics to propose the identity of androgen-regulated proteins and establish their hypothetical physiological role in the parasites. These analyses yield a possible explanation of how androgens exert their cysticidal effects through changes in the expression of proteins involved in cytoskeletal rearrangement, dynamic vesicular traffic and transduction of intracellular signals.


Asunto(s)
Andrógenos/farmacología , Muerte Celular , Proteoma , Taenia/efectos de los fármacos , Taenia/fisiología , Actinas/genética , Animales , Biología Computacional , Cisticercosis/patología , Cysticercus/efectos de los fármacos , Cysticercus/fisiología , Citoesqueleto/efectos de los fármacos , Citoesqueleto/genética , Dihidrotestosterona/farmacología , Femenino , Ratones , Ratones Endogámicos BALB C , Receptores de Progesterona/genética , Testosterona/farmacología , Tropomiosina/genética , Tubulina (Proteína)/genética
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