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J Clin Microbiol ; 45(7): 2309-11, 2007 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-17507516

RESUMEN

A total of 586 serum samples were used to evaluate the performance of type-specific herpes simplex virus type 2 (HSV-2) commercial enzyme-linked immunosorbent assays (ELISAs) by using the monoclonal antibody-blocking enzyme immunoassay (MAb-EIA) and a clinicovirological panel as reference standards. The Kalon and HerpeSelect ELISAs had similar sensitivities (93.5% and 93.8% compared with the results obtained by MAb-EIA, respectively, and 100% for both ELISAs compared with the results obtained with a clinicovirological panel). The Kalon ELISA had a higher specificity (96.5% and 96.8% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively) than the HerpeSelect ELISA (86.9% and 94% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively). A higher cutoff significantly improved the specificity of the HerpeSelect ELISA.


Asunto(s)
Anticuerpos Monoclonales/inmunología , Anticuerpos Antivirales/sangre , Ensayo de Inmunoadsorción Enzimática/métodos , Herpesvirus Humano 2/inmunología , Anticuerpos Antivirales/inmunología , Brasil/epidemiología , Herpes Genital/diagnóstico , Herpes Genital/epidemiología , Herpes Genital/virología , Humanos , Juego de Reactivos para Diagnóstico , Sensibilidad y Especificidad , Pruebas Serológicas/métodos
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