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The study of antioxidants is of pivotal importance in biomedicine as these molecules could be involved in biological pathways associated with disease. The identification of new antioxidants together with the acquisition of a deeper knowledge on their biology, could lead to the use of these compounds as drugs for innovative treatments. Plants are an important reservoir of phytodrugs that in many cases can be isolated with good extraction yields directly from the vegetal source and are often endowed with a low toxicity profile. Georgia, a country situated on the Black Sea coast in the Caucasus region at the intersection of Western Asia and Eastern Europe, is renowned for its unique woodland habitats and immense biological diversity due to the great variety of climate zones and landscapes. Many wild plants in the area are used as remedies for a number of illnesses in the local traditional medicine. However, the scientific knowledge of these sources of natural drugs and of their molecular components is still far from exhaustive. Therefore, with the present work we reviewed the scientific literature on some of the main Georgian medicinal plants and found that several species are a valuable source of hydrophilic and hydrophobic antioxidants, endowed in some cases with a high ROS-scavenging ability. The analysis of the literature also demonstrated that most of the medicinal extracts and compounds isolated from these plants are beneficial in suppressing multiple diseases in vitro. This review will provide information for scientists looking to develop secure plant-based pharmaceuticals as well as a rationale for using Georgian medicinal plants for the treatment of a range of diseases.
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Antioxidantes , Plantas Medicinales , Antioxidantes/química , Antioxidantes/farmacología , Antioxidantes/aislamiento & purificación , Plantas Medicinales/química , Humanos , Extractos Vegetales/química , Extractos Vegetales/farmacología , Extractos Vegetales/uso terapéutico , Georgia (República)RESUMEN
We investigate a quasi-2D suspension of Brownian particles in an optical speckle field produced by holographic manipulation of a laser wavefront. This system was developed to study, in a systematic and controllable way, a distinctive instance of diffusion, called Fickian yet Non Gaussian diffusion (FnGD), observed, during the last decade, for colloidal particles in a variety of complex and biological fluids. Our setup generates an optical speckle field that behaves like a disordered set of optical traps. First, we describe the experimental setup and the dynamics of the particles, focusing on mean square displacements, displacement distributions and kurtosis. Then, we present Brownian Dynamics simulations of point-like particles in a complex energy landscape, mimicking that generated by the optical speckle field. We show that our simulations can capture the salient features of the experimental results, including the emergence of FnGD, also covering times longer than the ones so far achieved in experiments. Some deviations are observed at long time only, with the Gaussian restoring being slower in simulations than in experiments. Overall, the introduced numerical model might be exploited to guide the design of upcoming experiments targeted, for example, to fully monitor the recovery of Gaussianity.
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Recent works show that glass-forming liquids display Fickian non-Gaussian Diffusion, with non-Gaussian displacement distributions persisting even at very long times, when linearity in the mean square displacement (Fickianity) has already been attained. Such non-Gaussian deviations temporarily exhibit distinctive exponential tails, with a decay length λ growing in time as a power-law. We herein carefully examine data from four different glass-forming systems with isotropic interactions, both in two and three dimensions, namely, three numerical models of molecular liquids and one experimentally investigated colloidal suspension. Drawing on the identification of a proper time range for reliable exponential fits, we find that a scaling law λ(t)âtα, with α≃1/3, holds for all considered systems, independently from dimensionality. We further show that, for each system, data at different temperatures/concentration can be collapsed onto a master-curve, identifying a characteristic time for the disappearance of exponential tails and the recovery of Gaussianity. We find that such characteristic time is always related through a power-law to the onset time of Fickianity. The present findings suggest that FnGD in glass-formers may be characterized by a "universal" evolution of the distribution tails, independent from system dimensionality, at least for liquids with isotropic potential.
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Vidrio , Difusión , Distribución NormalRESUMEN
Fickian yet non-Gaussian diffusion (FnGD), a most intriguing open issue in soft matter, is generically associated with some dynamical and/or structural heterogeneity of the environment. Here we investigate the features of FnGD in glass-forming liquids, the epitome of dynamical heterogeneity, drawing on experiments on hard-sphere colloidal suspensions and simulations of a simple model of molecular liquid. We demonstrate that FnGD strengthens on approaching the glass transition, by identifying distinct timescales for Fickianity, τ_{F}, and for restoring of Gaussianity, τ_{G}>τ_{F}, as well as their associated length scales, ξ_{F} and ξ_{G}. We find τ_{G}âτ_{F}^{γ} with γ≃1.8 for both systems. In the deep FnGD regime, the displacement distributions display exponential tails. We show that, in simulations, the time-dependent decay lengths l(t) at different temperatures all collapse onto a power-law master curve [l(t)/(ξ_{G})]â(t/τ_{G})^{α}, with α=0.33. A similar collapse, if less sharp, is also found in experiments, seemingly with the same exponent α. We further discuss the connections of the timescales and length scales characterizing FnGD with structural relaxation and dynamic heterogeneity.
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On approaching the glass transition, a dense colloid exhibits a dramatic slowdown with minute structural changes. Most microscopy experiments directly follow the motion of individual particles in real space, whereas scattering experiments typically probe the collective dynamics in reciprocal space at variable wavevector q. Multiscale studies of glassy dynamics are experimentally demanding and, thus, seldom performed. By using two-dimensional hard-sphere colloids at various area fractions Ï, we show here that Differential Dynamic Microscopy (DDM) can be effectively used to measure the collective dynamics of a glassy colloid in a range of q within a single experiment. As Ï is increased, the single decay of the intermediate scattering functions is progressively replaced by a more complex relaxation that we fit to a sum of two stretched-exponential decays. The slowest process, corresponding to the long-time particle escapes from caging, has a characteristic time τs = 1/(DLq2) with diffusion coefficient DLâ¼(Ïc-Ï)2.8, and Ïc ≃ 0.81. The fast process exhibits, instead, a non-Brownian scaling of the characteristic time τf(q) and a relative amplitude a(q) that monotonically increases with q. Despite the non-Brownian nature of τf(q), we succeed in estimating the short-time diffusion coefficient Dcage, whose Ï-dependence is practically negligible compared to the one of DL. Finally, we extend DDM to measure the q-dependent dynamical susceptibility χ4(q, t), a powerful yet hard-to-access multiscale indicator of dynamical heterogeneities. Our results show that DDM is a convenient tool to study the dynamics of colloidal glasses over a broad range of time and length scales.
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Glass transition is a most intriguing and long-standing open issue in the field of molecular liquids. From a macroscopic perspective, glass-forming systems display a dramatic slowing-down of the dynamics, with the inverse diffusion coefficient and the structural relaxation times increasing by orders of magnitude upon even modest supercooling. At the microscopic level, single-molecule motion becomes strongly intermittent, and can be conveniently described in terms of "cage-jump" events. In this work, we investigate a paradigmatic glass-forming liquid, the Kob-Andersen Lennard-Jones model, by means of Molecular Dynamics simulations, and compare the macroscopic and microscopic descriptions of its dynamics on approaching the glass-transition. We find that clear changes in the relations between macroscopic timescales and cage-jump quantities occur at the crossover temperature where Mode Coupling-like description starts failing. In fact, Continuous Time Random Walk and lattice model predictions based on cage-jump statistics are also violated below the crossover temperature, suggesting the onset of a qualitative change in cage-jump motion. Interestingly, we show that a fully microscopic relation linking cage-jump time- and length-scales instead holds throughout the investigated temperature range.
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Vidrio , Simulación de Dinámica Molecular , Vidrio/química , Movimiento (Física) , TemperaturaRESUMEN
Fickian yet non-Gaussian Diffusion (FnGD), widely observed for colloidal particles in a variety of complex and biological fluids, emerges as a most intriguing open issue in Soft Matter. To fully monitor FnGD and advance its understanding, recording many trajectories over a large time range is crucial, which makes experiments challenging. Here we exploit a recently introduced experimental model of finely tunable FnGD: a quasi-2d system of Brownian beads in water moving in a heterogeneous energy landscape generated by a static and spatially random optical force field (speckle pattern). By performing experiments at different optical power, we succeed in monitoring the evolution as well as the precursors of FnGD. Fickian scaling of the mean square displacement is always attained after a subdiffusive regime while the displacement distributions keep on being non-Gaussian, which allows for measuring a characteristic length- and time-scale for the onset of FnGD, ξf and tf. We find that ξf stays constant, whereas tf grows as the inverse of the long-time diffusion coefficient tf â D-1 for increasing the optical power. Deviations from the standard Gaussian shape of the displacement distribution are neatly characterized on a broad range of times, focusing on the excess probability at small displacements and on the decay-length of the distinctive exponential tails. Such deviations are fully built in the subdiffusive regime and, at the FnGD onset, grow with the optical power. As time goes on, the small-displacement probability narrows and the exponential tails progressively break up, with a tendency to recover the Gaussian behaviour. Overall, both subdiffusion and FnGD become more marked and persistent on increasing the optical power, suggesting a strict relation between these two regimes. As clearly demonstrated by our results, the adopted model-system represents a privileged stage for in-depth study of FnGD and opens the way to unveil the nature of this phenomenon through finely tuned and well-controlled experiments.
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Anomalous aging in soft glassy materials has generated a great deal of interest because of some intriguing features of the underlying relaxation process, including the emergence of "ultra-long-range" dynamical correlations. An intriguing possibility is that such a huge correlation length is reflected in detectable ensemble fluctuations of the macroscopic material properties. We tackle this issue by performing replicated mechanical and dynamic light scattering (DLS) experiments on alginate gels, which recently emerged as a good model-system of anomalous aging. Here we show that some of the monitored quantities display wide variability, including large fluctuations in the stress relaxation and the occasional presence of two-step decay in the DLS decorrelation functions. By quantifying elastic fluctuation through the standard deviation of the elastic modulus and dynamic heterogeneities through the dynamic susceptibility, we find that both quantities do increase with the gel age over a comparable range. Our results suggest that large elastic fluctuations are closely related to ultra-long-range dynamical correlation, and therefore may be a general feature of anomalous aging in gels.
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The breakdown of the Stokes-Einstein relation in supercooled liquids, which is the increase in the ratio τατD between the two macroscopic times for structural relaxation and diffusion on decreasing the temperature, is commonly ascribed to dynamic heterogeneities, but a clear-cut microscopic interpretation is still lacking. Here, we tackle this issue exploiting the single-particle cage-jump framework to analyze molecular dynamics simulations of soft disk assemblies and supercooled water. We find that τατDâ⟨tp⟩⟨tc⟩, where ⟨tp⟩ and ⟨tc⟩ are the cage-jump times characterizing slow and fast particles, respectively. We further clarify that this scaling does not arise from a simple term-by-term proportionality; rather, the relations ταâ⟨tp⟩⟨ΔrJ 2⟩ and τDâ⟨tc⟩⟨ΔrJ 2⟩ effectively connect the macroscopic and microscopic timescales, with the mean square jump length ⟨ΔrJ 2⟩ shrinking on cooling. Our work provides a microscopic perspective on the Stokes-Einstein breakdown and generalizes previous results on lattice models to the case of more realistic glass-formers.
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Crowding has a major impact on the dynamics of many material and biological systems, inducing effects as diverse as glassy dynamics and swarming. While this issue has been deeply investigated for a variety of living organisms, more research remains to be done on the effect of crowding on the behaviour of copepods, the most abundant metazoans on Earth. To this aim, we experimentally investigate the swimming behaviour, used as a dynamic proxy of animal adaptations, of males and females of the calanoid copepod Centropages typicus at different densities of individuals (10, 50 and 100 ind. l-1) by performing three-dimensional single-organism tracking. We find that the C. typicus motion is surprisingly unaffected by crowding over the investigated density range. Indeed, the mean square displacements as a function of time always show a crossover from ballistic to Fickian regime, with poor variations of the diffusion constant on increasing the density. Close to the crossover, the displacement distributions display exponential tails with a nearly density-independent decay length. The trajectory fractal dimension, D3D â 1.5, and the recently proposed 'ecological temperature' also remain stable on increasing the individual density. This suggests that, at least over the range of animal densities used, crowding does not impact on the characteristics of C. typicus swimming motion, and that a homeostatic mechanism preserves the stability of its swimming performance.
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Copépodos , Animales , Difusión , Femenino , Masculino , Natación , ZooplanctonRESUMEN
Chitosan (CS) is largely employed in environmental applications as an adsorbent of anionic dyes, due to the presence in its chemical structure of amine groups that, if protonated, act as adsorbing sites for negatively charged molecules. Efficient adsorption of both cationic and anionic dyes is thus not achievable with a pristine chitosan adsorbent, but it requires the combination of two or more components. Here, we show that simultaneous adsorption of cationic and anionic dyes can be obtained by embedding Linde Type A (LTA) zeolite particles in a crosslinked CS-based aerogel. In order to optimize dye removal ability of the hybrid aerogel, we target the crosslinker concentration so that crosslinking is mainly activated during the thermal treatment after the fast freezing of the CS/LTA mixture. The adsorption of isotherms is obtained for different CS/LTA weight ratios and for different types of anionic and cationic dyes. Irrespective of the formulation, the Langmuir model was found to accurately describe the adsorption isotherms. The optimal tradeoff in the adsorption behavior was obtained with the CS/LTA aerogel (1:1 weight ratio), for which the maximum uptake of indigo carmine (anionic dye) and rhodamine 6G (cationic dye) is 103 and 43 mg g-1, respectively. The behavior observed for the adsorption capacity and energy cannot be rationalized as a pure superposition of the two components, but suggests that reciprocal steric effects, chemical heterogeneity, and molecular interactions between CS and LTA zeolite particles play an important role.
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Aniones/química , Cationes/química , Quitosano/química , Colorantes/química , Geles/química , Purificación del Agua/métodos , Zeolitas/química , Adsorción , Reactivos de Enlaces Cruzados , Geles/síntesis química , Concentración de Iones de Hidrógeno , Cinética , Microscopía Electrónica de Rastreo , Modelos Químicos , Agua/química , Contaminantes Químicos del Agua/químicaRESUMEN
The recently discovered Fickian yet non-Gaussian diffusion (FnGD) is here finely tuned and investigated over a wide range of probabilities and timescales using a quasi-2D suspension of colloidal beads under the action of a static and spatially random optical force field. This experimental model allows one to demonstrate that a "rapid" FnGD regime with a diffusivity close to that of free suspension can originate from earlier subdiffusion. We show that these two regimes are strictly tangled: as subdiffusion deepens upon increasing the optical force, deviations from Gaussianity in the FnGD regime become larger and more persistent in time. In addition, the distinctive exponential tails of FnGD are quickly built up in the subdiffusive regime. Our results shed new light on previous experimental observations and suggest that FnGD may generally be a memory effect of earlier subdiffusive processes.
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We investigate via molecular dynamics simulations the behaviour of a polymer melt confined between surfaces with increasing spatial correlation (patchiness) of weakly and strongly interacting sites. Beyond a critical patchiness, we find a dramatic dynamic decoupling, characterized by a steep growth of the longest relaxation time and a constant diffusion coefficient. This arises from dynamic heterogeneities induced by the walls in the adjacent polymer layers, leading to the coexistence of fast and slow chain populations. Structural variations are also present, but they are not easy to detect. Our work opens the way to a better understanding of adhesion, friction, rubber reinforcement by fillers, and many other open issues involving the dynamics of polymeric materials on rough, chemically heterogeneous and possibly "dirty" surfaces.
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Suspensions of small planktonic copepods represent a special category in the realm of active matter, as their size falls within the range of colloids, while their motion is so complex that it cannot be rationalized according to basic models of self-propelled particles. Indeed, the wide range of individual variability and swimming patterns resemble the behaviour of much larger animals. By analysing hundreds of three-dimensional trajectories of the planktonic copepod Clausocalanus furcatus, we investigate the possibility of detecting how the motion of this species is affected by different external conditions, such as the presence of food and the effect of gravity. While this goal is hardly achievable by direct inspection of single organism trajectories, we show that this is possible by focussing on simple average metrics commonly used to characterize colloidal suspensions, such as the mean square displacement and the dynamic correlation functions. We find that the presence of food leads to the onset of a clear localization that separates a short-time ballistic from a long-time diffusive regime. Such a benchmark reflects the tendency of C. furcatus to remain temporally feeding in a limited space and disappears when food is absent. Localization is clearly evident in the horizontal plane, but is negligible in the vertical direction, due to the effect of gravity. Our results suggest that simple average descriptors may provide concise and useful information on the swimming properties of planktonic copepods, even though single organism behaviour is strongly heterogeneous.
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Copépodos/fisiología , Ecosistema , Natación , Zooplancton/fisiología , Animales , Fenómenos Biomecánicos , Coloides/química , Difusión , Movimiento (Física)RESUMEN
The dispersion of solid nanoparticles within polymeric materials is widely used to enhance their performance. Many scientific and technological aspects of the resulting polymer nanocomposites have been studied, but the role of the structural and chemical heterogeneity of the nanoparticles has just started to be appreciated. For example, simulations of polymer films on planar heterogeneous surfaces revealed unexpected, non-monotonic activation energy to diffusion on varying the surface composition. Motivated by these intriguing results, here we simulate via molecular dynamics a different, fully three-dimensional system, in which the heterogeneous nanoparticles are incorporated in a polymer melt. The nanoparticles are roughly spherical assemblies of strongly and weakly attractive sites, in fractions of f and 1 - f, respectively. We show that the polymer diffusion is still characterized by a non-monotonic dependence of the activation energy on f. The comparison with the case of homogeneous nanoparticles clarifies that the effect of the heterogeneity increases on approaching the polymer glass transition.
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Many amorphous materials show spatially heterogenous dynamics, as different regions of the same system relax at different rates. Such a signature, known as Dynamic Heterogeneity, has been crucial to understand the nature of the jamming transition in simple model systems and is currently considered very promising to characterize more complex fluids of industrial and biological relevance. Unfortunately, measurements of dynamic heterogeneities typically require sophisticated experimental set-ups and are performed by few specialized groups. It is now possible to quantitatively characterize the relaxation process and the emergence of dynamic heterogeneities using a straightforward method, here validated on video microscopy data of hard-sphere colloidal glasses. We call this method Differential Variance Analysis (DVA), since it focuses on the variance of the differential frames, obtained subtracting images at different time-lags. Moreover, direct visualization of dynamic heterogeneities naturally appears in the differential frames, when the time-lag is set to the one corresponding to the maximum dynamic susceptibility. This approach opens the way to effectively characterize and tailor a wide variety of soft materials, from complex formulated products to biological tissues.
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Glassy dynamics is intermittent, as particles suddenly jump out of the cage formed by their neighbors, and heterogeneous, as these jumps are not uniformly distributed across the system. Relating these features of the dynamics to the diverse local environments explored by the particles is essential to rationalize the relaxation process. Here we investigate this issue characterizing the local environment of a particle with the amplitude of its short time vibrational motion, as determined by segmenting in cages and jumps the particle trajectories. Both simulations of supercooled liquids and experiments on colloidal suspensions show that particles in large cages are likely to jump after a small time-lag, and that, on average, the cage enlarges shortly before the particle jumps. At large time-lags, the cage has essentially a constant size, which is smaller for longer-lasting cages. Finally, we clarify how this coupling between cage size and duration controls the average behavior and opens the way to a better understanding of the relaxation process in glass-forming liquids.
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Particles in structural glasses rattle around temporary equilibrium positions, that seldom change through a process which is much faster than the relaxation time, known as particle jump. Since the relaxation of the system is due to the accumulation of many such jumps, it could be possible to connect the single particle short time motion to the macroscopic relaxation by understanding the features of the jump dynamics. Here we review recent results in this research direction, clarifying the features of particle jumps that have been understood and those that are still under investigation, and examining the role of particle jumps in different theories of the glass transition.