A HD-Zip I transcription factor from physic nut, JcHDZ21, confers sensitive to salinity in transgenic Arabidopsis.

HD-Zip is a plant-specific transcription factor that plays an important regulatory role in plant growth and stress response. However, there have been few reports on the functions of members of the physic nut HD-Zip gene family. In this study, we cloned a HD-Zip I family gene from physic nut by RT-PCR, and named JcHDZ21. Expression pattern analysis showed that JcHDZ21 gene had the highest expression in physic nut seeds, and salt stress inhibited the expression of JcHDZ21 gene. Subcellular localization and transcriptional activity analysis showed that JcHDZ21 protein is localized in the nucleus and has transcriptional activation activity. Salt stress results indicated that JcHDZ21 transgenic plants were smaller and had more severe leaf yellowing compared to those of the wild type. Physiological indicators showed that transgenic plants had higher electrical conductivity and MDA content, and lower proline and betaine content compared with wild-type plants under salt stress. In addition, the expression of abiotic stress-related genes in JcHDZ21 transgenic plants was significantly lower than that in wild type under salt stress. Our results showed that ectopic expression of JcHDZ21 increased the sensitivity of transgenic Arabidopsis to salt stress. This study provides a theoretical basis for the future application of JcHDZ21 gene in the breeding of physic nut stress-tolerant varieties.

Cause Analysis and Preventive Measures of Guizhou D2809 Train Derailment Accident in Guizhou, China on 4 June 2022.

This paper summarizes the cause of the debris flow impact train accident by investigating the local geological condition, meteorological data and field investigation that happened in Guizhou, China on 4 June 2022. The result showed that the major reason is the continuous heavy rain in the surrounding area, which led to a small landslide at the upper right of the tunnel entrance. Besides, the construction of the Jianrong Expressway in the upper reaches increased the catchment area, which makes the water content of the upper soil increase while the shear strength decreases. Such large-scale catastrophic accidents significantly threaten the local environment and public safety. Therefore, it is urgent to pay special attention to the changes in geological conditions along the line, especially the adverse effects of construction, to improve the early risk warning and post-accident treatment ability.

A Brief Report on the Collapse of Self-Built Houses on 29 April 2022, in Changsha, China.

A self-built house collapsed in Changsha City, Hunan Province, on 29 April 2022, causing 53 people to be trapped and 39 people to go missing. The tragedy caused a huge loss of life, so the stability of self-built houses has a non-negligible impaction on public safety. This report presents causes of the incident, rescue operations, cause analysis, and the analytic hierarchy process (AHP). The main conclusions about the reasons for the collapse include: (a) reckless heightening, (b) unreasonable structure, (c) adverse geological conditions, (d) architectural vulnerabilities, (e) negligence of competent authorities, and (f) lack of security awareness. This paper provides a reference for avoiding similar security incidents in the future.