RESUMEN
Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.
Asunto(s)
Anticuerpos Antivirales/sangre , Ensayo de Inmunoadsorción Enzimática/métodos , Tamizaje Masivo/métodos , Pruebas de Neutralización/métodos , Pruebas Serológicas/métodos , Infección por el Virus Zika/diagnóstico , Virus Zika/inmunología , Anticuerpos Neutralizantes/sangre , Efecto Citopatogénico Viral , Humanos , Inmunoglobulina G/sangre , Martinica/epidemiología , Microscopía , Sensibilidad y Especificidad , Estudios Seroepidemiológicos , Infección por el Virus Zika/epidemiologíaRESUMEN
We describe the spread of a dengue virus during an outbreak in Saint Martin island (French West Indies) during winter 2003-2004. Dengue type 3 viruses were isolated from 6 patients exhibiting clinical symptoms. This serotype had not been detected on the island during the preceding 3 years. Genome sequence determinations and analyses showed a common origin with dengue type 3 viruses isolated in Martinique 2 years earlier.
Asunto(s)
Dengue/epidemiología , Dengue/virología , Brotes de Enfermedades , Virus del Dengue/genética , Humanos , Filogenia , Factores de Tiempo , Indias Occidentales/epidemiologíaRESUMEN
Dengue type 3 viruses were isolated from patients in Martinique between 1999 and 2002. This serotype had not been detected on the island in the last 20 years. Genomic sequence determination and analysis showed great stability of the virus during the period studied.