RESUMEN
Kombucha is a beverage made by fermenting sweetened tea with a symbiotic culture of yeast and bacteria. Literature data indicate that the kombucha beverage shows many health-promoting properties such as detoxification, chemo-preventive, antioxidant, antimicrobial, antifungal, and general strengthening. The research conducted focuses on the analysis of polyphenolic compounds formed in the fermentation process using ultra-efficient liquid chromatography, as well as on checking the antimicrobial properties of kombucha against pathogenic bacteria and yeasts found in food. Analysis of the composition of the tea mushroom (SCOBY) microflora using the MALDI TOF MS Biotyper mass spectrometer showed 8 species of bacteria and 7 species of yeasts. In vitro studies confirm the bactericidal and bacteriostatic properties of fermented kombucha beverages, with white and green tea beverages showing the highest antibacterial activity. The bacteria Staphylococcus aureus and yeast Candida albicans were the most sensitive to the effects of kombucha tea beverages. UPLC chromatographic analysis confirmed the presence of 17 bioactive compounds in kombucha beverages that can affect human health. The analyses conducted were aimed at indicating the best recipe and conditions to prepare a kombucha beverage, which allowed the selection of the version with the best health-promoting properties. Fermented kombucha teas contain many elements such as aluminium, calcium, iron, potassium, magnesium, sodium, phosphorus, and sulphur.
RESUMEN
OBJECTIVE: Introduction: Under the Act on State Emergency Medicine System, an emergency medical team consists of at least two members, including a specialist system nurse or paramedic, who have the license to perform medical rescue activities. However, this does not exclude the possibility of expanding the team. In practice, however, it appears that in most cases a head of healthcare institution decides to have teams with a number of members limited to two persons in which a paramedic or nurse also fulfil the function of a driver. PATIENTS AND METHODS: Materials rial and methods: An originally designed questionnaire was used to collect data. The sample group included 138 employees working in two-person emergency medical teams at the Regional Ambulance Station in Poznan. RESULTS: Results: Over half of the subjects (N=90; 65,2%) rarely requests assistance from another emergency medical team as support in intervention, however, when transporting patients such teams are often assisted by patients' families (N=129; 93,5%). The patient's weight is one of the most frequently cited reasons for using such assistance. Every third respondent knows standards on weight limits for manual transport. 77,5 % of respondents believe that the Act on State Emergency Medicine should be amended by increasing the minimum number of members in primary care emergency medical teams from two to three. CONCLUSION: Conclusions: It may be concluded that formation of two-person teams constitutes an infringement of occupational health and safety regulations on manual transport, which increases the risk of injury occurrence.
