In "Tone" with dogs: exploring canine musicality.

This study investigates the musical perception skills of dogs through playback experiments. Dogs were trained to distinguish between two different target locations based on a sequence of four ascending or descending notes. A total of 16 dogs of different breeds, age, and sex, but all of them with at least basic training, were recruited for the study. Dogs received training from their respective owners in a suitable environment within their familiar home settings. The training sequence consisted of notes [Do-Mi-Sol#-Do (C7-E7-G7#-C8; Hz frequency: 2093, 2639, 3322, 4186)] digitally generated as pure sinusoidal tones. The training protocol comprised 3 sequential training levels, with each level consisting of 4 sessions with a minimum of 10 trials per session. In the test phase, the sequence was transposed to evaluate whether dogs used relative pitch when identifying the sequences. A correct response by the dog was recorded as 1, while an incorrect response, occurring when the dog chose the opposite zone of the bowl, was marked as 0. Statistical analyses were performed using a binomial test. Among 16 dogs, only two consistently performed above the chance level, demonstrating the ability to recognize relative pitch, even with transposed sequences. This study suggests that dogs may have the ability to attend to relative pitch, a critical aspect of human musicality.

Human social buffer in goats and dogs.

The primary goal of this study was to explore the social buffering effect that humans offer to goats and dogs with limited exposure to human socialization, particularly in situations involving interactions with unfamiliar humans. A total of 13 dogs and 14 goats were selected for the study, all of which had limited prior socialization with humans. Each animal was placed in a testing room with unfamiliar humans for 15 min. Three experimenters aimed to establish a comfortable environment, encouraging social interaction by offering food to the animals and assessing the animals' willingness to accept food and their response to being approached and petted. If both conditions were satisfied, the animals were classified as "social". If one or none of the conditions were met, the animals were classified as "not social". Cortisol levels were measured by collecting blood samples before and after the test. Non-parametric tests together with a GzLM showed that the effect of human social buffering in goats was different in comparison to dogs: goats exhibited higher cortisol levels after the test, while dogs did not show a significant change. Further analysis demonstrated that "social" goats had a lower likelihood of experiencing significant changes in cortisol levels than dogs. Thus, once human interactions are accepted, both species could benefit from social buffering. In summary, this study enhances our understanding of how dogs and goats respond to social interactions with humans in the social buffering effect.

A Comparative Study of Dogs and Goats with Limited Human Socialization in the Impossible Task Paradigm.

The study aimed to explore how limited human socialization affects the socio-cognitive abilities and interactions with unfamiliar individuals of a selected group of domesticated dogs and goats. These animals were raised and kept under conditions characterized by limited human socialization, and their behavior was assessed using the "impossible task" paradigm. The study found that dogs, with a history of cooperative interactions and human companionship, exhibited more frequent social engagement with human experimenters in the experimental setting than goats, traditionally domesticated for utilitarian purposes. However, differences in interaction duration and latency were not significant, highlighting the complexity of these interactions. The results suggest that domestication history and behavioral ecology play significant roles in shaping animals' willingness to engage with humans. However, this study acknowledges limitations, such as the specific population studied, and calls for further research with larger and more diverse samples to generalize these findings. Understanding the interplay between domestication history, behavioral ecology, and human socialization could provide insights into the complex factors influencing animal-human interactions and cognitive behaviors, with implications for animal welfare and human-animal relationships.

Home Sweet Home: The Impact of Lifestyle on a Cat's Approach to Impossible Tasks in the Home Environment.

Cat welfare is a topic of growing interest in the scientific literature. Although previous studies have focused on the effects of living style (i.e., indoor/outdoor) on cat welfare, there has been a noticeable dearth of analysis regarding the impact of lifestyle on cats' inclination and mode of communication with humans. Our research aimed to analyze the possible effect of lifestyle (e.g., living indoors only or indoor/outdoor) on cat-human communication. The cats were tested using the impossible task paradigm test, which consists of some solvable trials in which the subject learns to obtain a reward from an apparatus, followed by an impossible trial through blocking the apparatus. This procedure triggers a violation of expectations and is considered a useful tool for assessing both the decision-making process and the tendency to engage in social behaviors towards humans. A specific ethogram was followed to record the behavioral responses of the cats during the unsolvable trial. Our results show the effects of lifestyle and age on domestic cats, providing valuable insights into the factors that influence their social behaviors. Cats that can roam freely outdoors spent less time interacting with the apparatus compared to indoor-only cats. Additionally, roaming cats showed stress behaviors sooner following the expectancy of violation compared to indoor cats. The lifestyle of cats can influence their problem-solving approach while not affecting their willingness to interact with humans or their overall welfare. Future studies on this topic can be useful for improving the welfare of domestic cats.

A novel telomerase activity and microRNA-21 upregulation identified in a family with palmoplantar keratoderma.

Palmoplantar keratoderma is a set of skin diseases with hyperkeratotic thickening of palms and soles which are characteristic of these heterogeneous group of keratinization disorders. Various genetic mutations, autosomal dominant or recessive, have been identified which may triggerpalmoplantar keratoderma, as KRT9 (Keratin 9), KRT1 (Keratin1), AQP5 (Aquaporin), SERPINB 7 (serine protease inhibitor). The identification of causal mutations is extremely important for the correct diagnosis. Here, we report the case of a family affected from Palmoplantar keratoderma caused by autosomal dominant KRT1 mutations (Unna-Thost disease). Telomerase activation and hTERT expression take a part in the process of cell proliferation and inflammation and microRNAs, as microRNA-21, are emerging as drivers in the regulation of telomerase activity. Here, the patients underwent KRT1 analysis genetic sequence, telomerase activity and miR-21 expression. Beside histopathology assay was performed. The patients presented thickening of the skin on soles of the feet and the palms of the hands, KRT1mutations and showed high expression levels of hTERT and hTR, the gene encoding for the telomeric subunits, and miR-21 (fold change > 1.5 and p value = 0.043), explicating the aberrant proliferation of epidermal layer and the inflammatory state characterizing palmoplantar keratoderma.

When are puppies receptive to emotion-induced human chemosignals? The cases of fear and happiness.

We report an observational, double-blind, experimental study that examines the effects of human emotional odors on puppies between 3 and 6 months and adult dogs (one year and upwards). Both groups were exposed to control, human fear, and happiness odors in a between subjects' design. The duration of all behaviors directed to the apparatus, the door, the owner, a stranger, and stress behaviors was recorded. A discriminant analysis showed that the fear odor activates consistent behavior patterns for both puppies and adult dogs. However, no behavioral differences between the control and happiness odor conditions were found in the case of puppies. In contrast, adult dogs reveal distinctive patterns for all three odor conditions. We argue that responses to human fear chemosignals systematically influence the behaviors displayed by puppies and adult dogs, which could be genetically prefigured. In contrast, the effects of happiness odors constitute cues that require learning during early socialization processes, which yield consistent patterns only in adulthood.

Serum Oxytocin, Cortisol and Social Behavior in Calves: A Study in the Impossible Task Paradigm.

In this study, we explored the correlations between circulating levels of oxytocin, cortisol, and different social behaviors toward humans in 26 Italian Red Pied calves (all females, with an average age of 174 ± 24 days) using the impossible task paradigm. This paradigm has proved fruitful in highlighting the effect of socialization on the willingness to interact with humans in several domesticated species. The test consists of the violation of an expectation (recovering food from an experimental apparatus) while a caregiver and a stranger are present. Immediately after the end of the test (less than one minute), blood was collected from the coccygeal vein. Statistics were performed by the Spearman's rank correlation; significant differences were adjusted according to Bonferroni's correction. Cortisol correlates positively (ρ = 0.565; p < 0.05) with the latency of behaviors directed at the caregiver, and the duration of behaviors directed at the apparatus correlates negatively with both the caregiver (ρ = -0.654; p < 0.05) and a stranger (ρ = -0.644; p < 0.05). Contrary to what is reported in the literature on cows, no correlations were found between oxytocin levels and direct behaviors toward the caregiver. This highlights a different behavioral strategy between calves and cows when placed in front of an impossible task.

Puppies in the problem-solving paradigm: quick males and social females.

We report an observational, double-blind study that examined puppies' behaviors while engaged in solving an experimental food retrieval task (food retrieval task instrument: FRTI). The experimental setting included passive social distractors (i.e., the dog's owner and a stranger). The focus was on how the social and physical environment shapes puppies' behaviors according to sex. The dependent variables were the number of tasks solved on an apparatus (Performance Index) and the time required to solve the first task (Speed). Sex and Stress were set as explanatory factors, and Social Interest, FRTI interactions, other behavior, and age as covariates. The main findings were that male puppies solved the first task faster than females. On the other hand, females displayed significantly more social interest and did so more rapidly than males. Males showed delayed task resolution. This study demonstrates sex differences in a problem-solving task in dog puppies for the first time, thus highlighting that sexually dimorphic behavioral differences in problem-solving strategies develop early on during ontogenesis.

Comparative insights of the neuroanatomical distribution of the gonadotropin-inhibitory hormone (GnIH) in fish and amphibians.

This paper intends to apprise the reader regarding the existing knowledge on the neuroanatomical distribution of GnIH-like peptides in in fish and amphibians in both the adult stage and during ontogenesis. The neuroanatomical distribution of GnIH-like neuropeptides appears quite different in the studied species, irrespective of the evolutionary closeness. The topology of the olfactory bulbs can affect the distribution of neurons producing the GnIH-like peptides, with a tendency to show a more extended distribution into the brains with pedunculate olfactory bulbs. Therefore, the variability of the GnIH-like system could also reflect specific adaptations rather than evolutionary patterns. The onset of GnIH expression was detected very early during development suggesting its precocious roles, and the neuroanatomical distribution of GnIH-like elements showed a generally increasing trend. This review highlights some critical technical aspects and the need to increase the number of species to be studied to obtain a complete neuroanatomical picture of the GnIH-like system.

Serum Oxytocin in Cows Is Positively Correlated with Caregiver Interactions in the Impossible Task Paradigm.

This study explored a possible relationship between the circulating oxytocin, cortisol, and the willingness of dairy cows to engage in social behaviors with humans in an experimental context. The behaviors of twenty-nine cows were recorded during the impossible task paradigm, a procedure aimed at creating a violation of expectancy, in the presence of the caregiver and a stranger. The results showed that serum oxytocin levels were positively correlated with duration and negatively correlated with the latency of the cows' social interactions with the caregiver. This research provides a clear correlation between circulating oxytocin and a willingness to engage in social contact with the caregiver, excluding the possible effect of different cortisol levels on such behavior.

Is this love? Sex differences in dog-owner attachment behavior suggest similarities with adult human bonds.

Sex differences in the behavioral responses of Labrador Retriever dogs in the Strange Situation Test were explored. Behaviors expressed by dogs during seven 3-min episodes were analyzed through a Principal Component Analysis (PCA). The scores of factors obtained were analyzed with a Generalized Linear Mixed Model to reveal the effects of the dog's sex and age and the owner's sex. In Episode 1 (dog and owner) and 5 (dog alone), the PCA identified three and two factors, respectively, which overall explained 68.7% and 59.8% of the variance, with no effect of sex. In Episodes 2 (dog, owner, and stranger), 3 and 6 (dog and stranger), and 4 and 7 (dog and owner), the PCA identified four factors, which overall explained 51.0% of the variance. Effects of sex were found on: Factor 1 (distress), with lower scores obtained by females in Episode 2 and higher in Episode 3; Factor 2 (sociability), which was overall higher in females; Factor 3 (separation-distress), with females, but not males, obtaining higher scores when left with the stranger than when with the owner. Therefore, females were overall more social but seemed more affected than males by the owner's absence. Parallels can be traced between our results and sex differences found in adult human romantic attachment, suggesting that the dog-owner bond has characteristics that are not found in the infant-mother relationship.

Research Trends in Octopus Biological Studies.

Octopuses represent interesting model studies for different fields of scientific inquiry. The present study provides a bibliometric analysis on research trends in octopuses biological studies. The analysis was executed from January 1985 to December 2020 including scientific products reported in the Web of Science database. The period of study was split into two blocks ("earlier period" (EP): 1985-2010; "recent period" (RP): 2011-2020) to analyze the evolution of the research topics over time. All publications of interest were identified by using the following query: ((AK = octopus) OR (AB = octopus) OR (TI = octopus)). Data information was converted into an R-data frame using bibliometrix. Octopuses studies appeared in 360 different sources in EP, while they increased to 408 in RP. Sixty countries contributed to the octopuses studies in the EP, while they were 78 in the RP. The number of affiliations also increased between EP and RP, with 835 research centers involved in the EP and 1399 in the RP. In the EP 5 clusters (i.e., "growth and nutrition", "pollution impact", "morphology", "neurobiology", "biochemistry") were represented in a thematic map, according to their centrality and density ranking. In the RP the analysis identified 4 clusters (i.e., "growth and nutrition", "ecology", "pollution impact", "genes, behavior, and brain evolution"). The UK with Ireland, and the USA with Canada shared the highest number of publications in the EP, while in the RP, Spain and Portugal were the leading countries. The current data provide significant insight into the evolving trends in octopuses studies.

Sex differences in the behavioral responses of dogs exposed to human chemosignals of fear and happiness.

This research focuses on sex differences in the behavioral patterns of dogs when they are exposed to human chemosignals (sweat) produced in happy and fear contexts. No age, breed or apparatus-directed behavior differences were found. However, when exposed to fear chemosignals, dogs' behavior towards their owners, and their stress signals lasted longer when compared to being exposed to happiness as well as control chemosignals. In the happy odor condition, females, in contrast to males, displayed a significantly higher interest to the stranger compared to their owner. In the fear condition, dogs spent more time with their owner compared to the stranger. Behaviors directed towards the door, indicative of exit interest, had a longer duration in the fear condition than the other two conditions. Female dogs revealed a significantly longer door-directed behavior in the fear condition compared to the control condition. Overall the data shows that the effect of exposure to human emotional chemosignals is not sex dependent for behaviors related to the apparatus, the owner or the stress behaviors; however, in the happiness condition, females showed a stronger tendency to interact with the stranger.

The scholar's best friend: research trends in dog cognitive and behavioral studies.

In recent decades, cognitive and behavioral knowledge in dogs seems to have developed considerably, as deduced from the published peer-reviewed articles. However, to date, the worldwide trend of scientific research on dog cognition and behavior has never been explored using a bibliometric approach, while the evaluation of scientific research has increasingly become important in recent years. In this review, we compared the publication trend of the articles in the last 34 years on dogs' cognitive and behavioral science with those in the general category "Behavioral Science". We found that, after 2005, there has been a sharp increase in scientific publications on dogs. Therefore, the year 2005 has been used as "starting point" to perform an in-depth bibliometric analysis of the scientific activity in dog cognitive and behavioral studies. The period between 2006 and 2018 is taken as the study period, and a backward analysis was also carried out. The data analysis was performed using "bibliometrix", a new R-tool used for comprehensive science mapping analysis. We analyzed all information related to sources, countries, affiliations, co-occurrence network, thematic maps, collaboration network, and world map. The results scientifically support the common perception that dogs are attracting the interest of scholars much more now than before and more than the general trend in cognitive and behavioral studies. Both, the changes in research themes and new research themes, contributed to the increase in the scientific production on the cognitive and behavioral aspects of dogs. Our investigation may benefit the researchers interested in the field of cognitive and behavioral science in dogs, thus favoring future research work and promoting interdisciplinary collaborations.

Celebrating 50+ years of research on the reproductive biology and endocrinology of the green frog: An overview.

This issue is dedicated to the late Professor Giovanni Chieffi, and this article is an overview of the research on Comparative Endocrinology of reproduction using Rana esculenta (alias Pelophylax esculentus) as a model system. Starting from the early 1970s till today, a large quantity of work have been conducted both in the fields of experimental endocrinology and in the definition of the diffuse neuroendocrine system, with a major focus on the increasing role of regulatory peptides. The various aspects investigated concerned the histological descriptions of principal endocrine glands of the hypothalamic-pituitary-gonadal (HPG) axis, the localization and distribution in the HPG of several different substances (i.e. neurosteroids, hypothalamic peptide hormones, pituitary gonadotropins, gonadal sex steroids, and other molecules), the determination of sex hormone concentrations in both serum and tissues, the hormone manipulations, as well as the gene and protein expression of steroidogenic enzymes and their respective receptors. All together these researches, often conducted considering different periods of the annual reproductive cycle of the green frog, allowed to understand the mechanism of cascade control/regulation of the HPG axis of R. esculenta, characterizing the role of different hormones in the two sexes, and testing the hypotheses about the function of single hormones in different target organs. It becomes evident from the review that, in their simplest form, several features of this species are specular as compared to those of other vertebrate species and that reproduction in this frog species is either under endogenous multi-hormonal control or by a wide array of different factors. Our excursus of this research, spanning almost five decades, shows that R. esculenta has been intensively and successfully used as an animal model in reproductive endocrinology as well as several field studies such as those involving environmental concerns that focus on the effects of endocrine disruptors and other environmental contaminants.

Multimodal signaling in the visuo-acoustic mismatch paradigm: similarities between dogs and children in the communicative approach.

In this review, we have analyzed the studies on the "mismatch paradigm" or "contrasting paradigm", in which the word indicates an intent that is opposite to the gesture in dogs and children. The studies on children highlighted the importance of the type of gestural messages that, when delivered in a non-ostensive manner, assume less value than the verbal indication; whereas, when more emphasis is given to the gestures, it produces opposite results. Word-trained dogs appear to rely more on words, but in the absence of such specific training, dogs rely more on gestures either in transitive or intransitive actions. Moreover, gestural communication appears easier to generalize, since dogs respond equally well to the gestural messages of familiar persons and strangers, whereas their performance lowers when a stranger provides a vocal message. Visual signals trigger faster responses than auditory signals, whereas verbal indications can at most equal the gestural latencies, but never overcome them. Female dogs appeared to be more proficient in the interpretation of gestural commands, while males performed better in the case of verbal commands. Based on a PRISMA analyses from the Web of Science database, three papers on children and four on dogs were retrieved. Our analyses revealed that gestures are more reliable reference points than words for dogs and children. Future studies should focus on choices related to objects of different values for the subjects. Moreover, the choices of dogs should be compared using known and unknown objects, which might help clarify how familiarity with the objects could differently influence their responses.

Distribution of gonadotropin-inhibitory hormone (GnIH)-like immunoreactivity in the brain and pituitary of the frog (Pelophylax esculentus) during development.

Gonadotropin-inhibitory hormone (GnIH) is a newly discovered hypothalamic RFamide peptide that influences reproduction by regulating brain and pituitary neuroendocrine functions in vertebrates. We report here for the first time, the ontogenetic description of GnIH-like immunoreactivity in the brain, olfactory system, and pituitary of the frog, Pelophylax esculentus. GnIH-like immunoreactive (GnIH-ir) elements were first observed in larvae at stage 24 in the olfactory mucosa, ventral telencephalon, and diencephalon. GnIH-ir-positive staining progressively increased in frequency and intensity during larval growth and other ir perikarya appeared in the medial septum, anterior commissure, dorsal hypothalamus, and posterior tuberculum. A decline in GnIH-ir neurons was seen along the olfactory/vomeronasal/terminal nerve complex in the stages following the pre- and prometamorphosis, while other GnIH-ir neurons showed positivity in the ventromedial surface of the olfactory bulbs and into the habenular nuclei, but the latter are no longer observed in the following stages of development. The anterior-posterior axis in several brain areas, along with the median eminence and pars intermedia of the hypophysis had the appearance of GnIH-ir fibers from early stages, with a progressive increase in the number till metamorphosis in all major subdivisions of the brain. After premetamorphosis, GnIH-ir fibers arising from labeled neurons in the suprachiasmatic nucleus could be seen contacting the ventricular lumen. The transient appearance of GnIH-ir elements in the olfactory system may hint at an olfactory placode origin in the extracranial region. The distribution of GnIH in several brain regions throughout development suggests important involvement of GnIH in multiple brain functions during development.

Effects of chemical pollutants on reproductive and developmental processes in Italian amphibians.

It is a widely held belief that environmental contaminants contribute to the decline of amphibian populations. By spending most of their early life in water and later stages on the land, amphibians face a constant risk of exposure to pesticides and other chemical pollutants in both aquatic and terrestrial environments. This review presents an overview of the studies carried out in Italian amphibians to highlight hazardous effects of bioaccumulation of chemical pollutants in juveniles and adults in various contaminated environments. Further, the studies in the laboratory setting assessing the effects of chemical pollutants on reproductive and developmental processes are reported. These studies and their relative references have been summarized in a tabular form. Three prominent contaminant groups were identified: herbicides, insecticides, and fungicides; and only a few works reported the effects of other chemical pollutants. Each pollutant group has been delegated to a section. All through the literature survey, it is seen that interest in this topic in Italy is very recent and sparse, where only a few anuran and caudata species and only some chemical pollutants have been studied.