Biomimetic Chemical Reactions with Natural Products Using Metalloporphyrins and Salen Complexes as Catalysts: A Brief Review.

The cytochrome P450 is a superfamily of hemoproteins mainly present in the liver and are versatile biocatalysts. They participate in the primary metabolism and biosynthesis of various secondary metabolites. Chemical catalysts are utilized to replicate the activities of enzymes. Metalloporphyrins and Salen complexes can contribute to the products' characterization and elucidate biotransformation processes, which are investigated during pre-clinical trials. These catalysts also help discover biologically active compounds and get better yields of products of industrial interest. This review aims to investigate which natural product classes are being investigated by biomimetic chemical models and the functionalities applied in the use of these catalysts. A limited number of studies were observed, with terpenes and alkaloids being the most investigated natural product classes. The research also revealed that Metalloporphyrins are still the most popular in the studies, and the identity and yield of the products obtained depend on the reaction system conditions.

Wastewater surveillance to assess cocaine and methylenedioxymethamphetamine use trends during a major music festival in Brazil.

Wastewater-based epidemiology was applied in northeastern Brazil during a dance festival, revealing that cocaine consumption doubled during the event days. The daily drug loads were 0.95 ± 0.03 to 11.4 ± 0.4 g/day for BE, 1.8 ± 0.4 to 7.6 ± 0.3 g/day for COC, 0.04 ± 0.02 to 0.19 ± 0.02 g/day for COE, and 0.08 ± 0.02 to 0.80 ± 0.02 g/day for MDMA.

This study employed the wastewater­based epidemiology (WBE) strategy to investigate cocaine (COC) and ecstasy (MDMA) usage during Carnatal festival in Rio Grande do Norte, Brazil. Wastewater samples were collected over the four festival days (December 12­15, 2019), as well as during corresponding days before and after the event. The samples underwent filtration, acidification, solid­phase extraction (SPE) for pre­concentration, and analysis using ultra­high­performance liquid chromatography­tandem mass spectrometry (UHPLC­MS/MS) with calibration using deuterated internal standards. The measured concentration ranges in the wastewater samples were as follows: benzoylecgonine (BE, a metabolite of COC use) ranged from 760 ± 30 to 8914 ± 252 ng/L, COC (non­metabolized or directly discharged) ranged from 1182 ± 25 to 6,760 ± 95 ng/L, cocaethylene (COE, a metabolite of alcohol­COC use) ranged from 21 ± 10 to 161 ± 10 ng/L, and MDMA (ecstasy) ranged from 18 ± 5 to 613 ± 15 ng/L. Daily wastewater loads were estimated as 0.81 ± 0.03 to 11.69 ± 0.33 g/day for BE, 1.42 ± 0.03 to 8.06 ± 0.11 g/day for COC, 0.02 ± 0.01 to 0.21 ± 0.01 g/day for COE, and 0.02 ± 0.01 to 0.80 ± 0.02 g/day for MDMA. Notably, COE and MDMA were only detected above the quantification limit during the festival days. The data enable estimating that COC consumption doubled during the event days. The COC/BE ratio indicated likely drug disposals on the event's last day and the subsequent week. The study's findings shed light on illicit substance consumption, assisting in public health programs and combating drug trafficking efforts.

Through screens and windows: remote work of university professors / Pela tela, pela janela: trabalho remoto de professores universitários / A través de pantallas y ventanas: trabajo remoto de profesores universitarios

Abstract The COVID-19 pandemic necessitated a shift to remote education, significantly impacting teachers and students. This study, the second phase of a longitudinal research project, examines the work context of university professors during the pandemic. Focusing on changes in their work environment, the study involved interviews with three professors, analyzed from a constructivist-interpretative perspective. Key findings indicate a merging of home and work spaces, overlapping professional and personal time, and new costs associated with remote work. The findings also showed increased administrative responsibilities and a lack of institutional support. Furthermore, the study observed reduced work-related interactions and rest periods, counterbalanced by improved flexibility and institutional communication. These insights deepen the understanding of teaching dynamics during the pandemic and its broader life impacts. However, limitations included the study's small participant number and their single-course affiliation. Future research could expand to diverse courses and a larger pool of participants. Investigating female teachers' reproductive work to address gender inequality in higher education is also an important goal.

Resumo A pandemia da COVID-19 exigiu uma mudança para o ensino remoto, impactando significativamente professores e alunos. Este estudo, segunda fase de um projeto de pesquisa longitudinal, examina o contexto de trabalho de professores universitários durante a pandemia. Com foco nas mudanças no ambiente de trabalho, o estudo envolveu entrevistas com três professores, analisadas sob uma perspectiva construtivo-interpretativa. As principais conclusões indicam uma fusão dos espaços de casa e de trabalho, sobreposição de tempo profissional e pessoal e novos custos associados ao trabalho remoto. As conclusões também mostraram maiores responsabilidades administrativas e falta de apoio institucional. Além disso, o estudo observou uma redução nas interações relacionadas ao trabalho e nos períodos de descanso, contrabalançada pela melhoria da flexibilidade e da comunicação institucional. Estas percepções aprofundam a compreensão da dinâmica do ensino durante a pandemia e dos seus impactos mais amplos na vida. No entanto, as limitações incluíram o pequeno número de participantes do estudo e sua afiliação a um único curso. Pesquisas futuras poderiam se expandir para diversos cursos e um grupo maior de participantes. Investigar o trabalho reprodutivo das professoras para abordar a desigualdade de gênero no ensino superior também é um objetivo importante.

Resumen La pandemia de COVID-19 llevó a un cambio a la educación a distancia, impactando a docentes y estudiantes. Este estudio es la segunda fase de una investigación longitudinal sobre el contexto laboral de los profesores universitarios en este periodo. Mediante entrevistas a tres profesores y un enfoque constructivo-interpretativo, se identificó la fusión de espacios de trabajo y hogar, la mezcla de tiempos profesionales y personales y costos adicionales del trabajo remoto. Se notó un aumento en tareas administrativas y falta de apoyo de las instituciones. Hubo una disminución en las interacciones laborales y descansos, aunque se ganó en flexibilidad y comunicación institucional. Esto aporta a entender la dinámica docente en pandemia y sus efectos en la vida. Las limitaciones del estudio incluyen el número reducido de participantes y su concentración en una sola área académica. Futuras investigaciones podrían diversificar cursos y participantes, y estudiar la carga de trabajo doméstico de las docentes para enfrentar la desigualdad de género en la educación superior.

Perfil de Egresos de Psicología de una Universidad del Interior Cearense / Perfil de egressos de psicologia de uma universidade do interior cearense / Profile of psychology graduates from a university in the countryside of the state of ceará

RESUMO O objetivo deste estudo foi analisar o perfil dos egressos do curso de Psicologia da Universidade Federal do Ceará, campus Sobral. Realizou-se um levantamento por meio de questionário autoaplicável (n = 157). A maioria dos participantes era do gênero feminino e tinha entre 20 e 30 anos. Os dados foram analisados com o programa IBM SPSS (versão 22). Foram produzidas estatísticas descritivas. Dentre os achados, viu-se que a maioria dos egressos nascera e residia na macrorregião de Sobral/Ibiapaba. Grande parte dos egressos trabalhava como psicólogo e a clínica foi a área de atuação mais citada. A principal dificuldade para a inserção no mercado de trabalho foi a reduzida experiência profissional. Depreende-se que a interiorização das universidades está relacionada à inclusão social ao possibilitar o acesso à educação e a fixação de profissionais no interior dos estados.

RESUMEN El propósito de la presente investigación fue examinar el perfil de los egresados de la carrera de Psicología en la Universidad Federal de Ceará, ubicada en el campus de Sobral. Para ello, se efectuó una recolección de datos mediante un cuestionario autoadministrado, el cual fue completado por un total de 157 participantes. La mayoría de estos eran mujeres, con edades comprendidas entre los 20 y 30 años. El análisis de los datos recabados se llevó a cabo utilizando el software IBM SPSS, versión 22, generando estadísticas descriptivas. Entre los hallazgos, la mayoría de los egresados son originarios y residentes de la macrorregión de Sobral/Ibiapaba. Se observó que un número considerable de los graduados se desempeña profesionalmente como psicólogos, siendo la práctica clínica el ámbito más frecuentemente señalado de actuación. Uno de los principales obstáculos identificados para la inserción en el mercado laboral fue la limitada experiencia profesional de los egresados. A partir de estos resultados, se infiere que la descentralización de las universidades cumple un papel fundamental en la inclusión social, al facilitar el acceso a la educación superior y promover la retención de profesionales en las áreas más alejadas de los centros urbanos.

ABSTRACT The purpose of this study was to examine the demographic and professional characteristics of Psychology graduates from the Federal University of Ceará's Sobral campus. Conducted via a self-administered questionnaire with 157 respondents, the survey found that a predominant number of participants were female, aged 20 to 30 years. Data analysis was performed using IBM SPSS software (version 22), generating descriptive statistics. Results indicated that most graduates originated from and resided within the Sobral/Ibiapaba macro-region, with a significant number employed as psychologists, particularly in clinical practice. The primary barrier to job market entry identified was limited professional experience. This study suggests that the decentralization of higher education contributes to social inclusion, promoting both access to education and the retention of professionals in less urbanized areas.

Lacticaseibacillus casei decrease long-chain fatty acids and most substances in an experimental model of intestinal mucositis.

PURPOSE: To evaluate the long-chain fatty acid and major compounds levels in the feces after prophylactic oral use of Lacticaseibacillus casei in an experimental model of intestinal mucositis. METHODS: Fifteen Swiss mice were randomly divided into three groups (n=5/group): The negative or positive control groups (n = 5) received saline orally for 18 days and an the intraperitoneal (i.p.) of saline or 5 Fluorouracil (450 mg/kg) in 15th day, respectability. L. casei group received oral concentration of L. casei (1x109 CFU/mL) for 18 days, the i.p. injection of 5-fluorouracil (450 mg/kg) in 15th days. Tissue samples from colon and each small intestine segment were collected for histopathological analysis. Stool samples were collected. Fecal composition of long-chain fatty acids and sterols were analysed by gas chromatography-mass spectrometry on the 15th and the 18th day. RESULTS: The mucosa layer of all small intestine segments of animals from L. casei showed well preserved epithelium and glands, without necrosis signs, but Goblet cells number decreased. Several long-chain fatty acids and sterols have been identified before and after in the groups. L. casei administration after 5-FU treatment reduced concentrations of linoleic acid (18:2) (p < 0.001) and oleic acid (18:1) (p < 0.001) in feces. CONCLUSIONS: L. casei prevented the mucosal damage associated with 5-FU-induced intestinal mucositis reduced long-chain fatty acid levels in the feces.

Abusive use of Zolpidem as a Result of COVID-19 and Perspectives of Continuity of the Problem in the Post-Pandemic Period.

Zolpidem is a non-benzodiazepine hypnotic drug that works as a positive modulator of Gamma-Amino Butyric Acid-A (GABA-A) receptors, with high selectivity for α1 subunits. Given this selective binding, the drug has a strong hypnotic activity. Social isolation during the SARS-CoV-2 pandemic has contributed to increased rates of anxiety, depression, and insomnia. As a result, studies have pointed to a possible increase in the indiscriminate use of drugs with sedative effects, such as Zolpidem, during the pandemic. The aim of this work was to present prospective evidence that warns of the possibility of the abusive use of Zolpidem even after the pandemic. High rates of addiction to this drug have been reported around the world after the emergence of the coronavirus. Data from the National Survey on Drug Use and Health and from Medicaid support the continuing growth in prescription and indiscriminate use of Zolpidem during the pandemic and afterward. Therefore, there is enough evidence to support the indiscriminate use of this drug since the beginning of the pandemic. Rates of indiscriminate use of sedatives may continue to increase in the post-pandemic period, especially if strict control measures are not taken by health authorities.

Mental health outcomes in frontline healthcare workers in Brazil during the COVID-19 epidemic: Results of an online survey in four regions using respondent-driven sampling (RDS).

BACKGROUND: The COVID-19 pandemic overwhelmed health facilities and presented healthcare workers (HCWs) with a new infectious disease threat. In addition to a sanitary crisis, Brazil still had to face major political, economic, and social challenges. This study aimed to investigate mental health outcomes in frontline HCWs in different regions of the country and at different epidemic times. We also sought to identify the main risk factors associated with these outcomes. METHODS: A cross-sectional online survey using respondent-driven sampling was conducted to recruit physicians (n = 584), nurses (n = 997), and nurse technicians (n = 524) in 4 regions of Brazil (North, Northeast, Southeast, and South) from August 2020 to July 2021. We used standardized instruments to screen for common mental disorders (CMD)(SRQ-20), alcohol misuse (AUDIT-C), depression (PHQ-9), anxiety (GAD-7), and post-traumatic stress disorder (PTSD)(PCL-5). Gile's successive sampling estimator was used to produce weighted estimates. We created a three-cluster data set for each HCW category and developed a hierarchical regression model with three levels: individual characteristics; workplace-related aspects; COVID-19 personal experience. The impact of the epidemic moment on the outcomes was also studied. RESULTS: The prevalence of probable CMD was 26.8-36.9%, alcohol misuse 8.7-13.6%, depression 16.4-21.2%, anxiety 10.8-14.2%, and PTSD 5.9-8.0%. We found a stronger association between mental health outcomes and the following factors: history of psychiatric disorders, female gender, and clinical comorbidities (level 1); work overload and family isolation (level 2); sick leave (level 3). Epidemic variables, such as the number of deaths and trend of deaths by COVID-19, had almost no impact on the outcomes. CONCLUSION: An alarmingly high prevalence of depression and anxiety was found in Brazilian frontline HCWs. Individual factors were the most strongly associated with mental health outcomes. These findings indicate the need to develop programs that provide emotional support, identify professionals at risk and refer them to specialized treatment when necessary.

Vitamin E and Its Molecular Effects in Experimental Models of Neurodegenerative Diseases.

With the advancement of in vivo studies and clinical trials, the pathogenesis of neurodegenerative diseases has been better understood. However, gaps still need to be better elucidated, which justifies the publication of reviews that explore the mechanisms related to the development of these diseases. Studies show that vitamin E supplementation can protect neurons from the damage caused by oxidative stress, with a positive impact on the prevention and progression of neurodegenerative diseases. Thus, this review aims to summarize the scientific evidence of the effects of vitamin E supplementation on neuroprotection and on neurodegeneration markers in experimental models. A search for studies published between 2000 and 2023 was carried out in the PubMed, Web of Science, Virtual Health Library (BVS), and Embase databases, in which the effects of vitamin E in experimental models of neurodegeneration were investigated. A total of 5669 potentially eligible studies were identified. After excluding the duplicates, 5373 remained, of which 5253 were excluded after checking the titles, 90 articles after reading the abstracts, and 11 after fully reviewing the manuscripts, leaving 19 publications to be included in this review. Experiments with in vivo models of neurodegenerative diseases demonstrated that vitamin E supplementation significantly improved memory, cognition, learning, motor function, and brain markers associated with neuroregeneration and neuroprotection. Vitamin E supplementation reduced beta-amyloid (Aß) deposition and toxicity in experimental models of Alzheimer's disease. In addition, it decreased tau-protein hyperphosphorylation and increased superoxide dismutase and brain-derived neurotrophic factor (BDNF) levels in rodents, which seems to indicate the potential use of vitamin E in preventing and delaying the progress of degenerative lesions in the central nervous system.

Neuroprotective Potential of Seed Extracts: Review of In Vitro and In Vivo Studies.

INTRODUCTION: Neurodegenerative diseases are characterized by neuronal dysfunction and death. Studies suggest that some seed extracts have a neuroprotective effect. Considering the increased incidence of these diseases and the need for new effective therapies with fewer side effects, this review aimed to assess the evidence of the efficacy and safety of seed extracts in experimental models of neurodegeneration. MATERIAL AND METHOD: The search was carried out through studies published between 2000 and 2021 in Science Direct, PubMed, Scientific Electronic Library Online (SciELO), and Latin American Literature in Health Sciences (LILACS) databases, in which the effects of seed extracts in in vitro and in vivo experimental models of neurodegeneration were investigated. Based on the eligibility criteria, 47 studies were selected for this review. RESULTS: In the in vitro models, the neuroprotection of the seed extracts was a result of their antioxidant, anti-inflammatory, and anti-apoptotic properties. In the in vivo models, neuroprotection resulted from the antioxidant and anti-inflammatory properties, a decrease in motor deficits, an improvement in learning and memory, as well as the increased release of neurotransmitters. The results show promise for the future of clinical research on new therapies for neurodegenerative diseases. However, the studies are still limited, which does not allow us to extrapolate the results to human beings with ND. CONCLUSIONS: Therefore, clinical trials are needed in order to prove the results of the in vitro and in vivo studies, as well as to assess the ideal, safe, and effective dose of these seed extracts in patients with neurodegenerative diseases.

Trabalho Docente no Contexto da Expansão Universitária / Teaching Work in the Context of University Expansion / Trabajo Docente en el Contexto de la Expansión Universitaria

As reformas neoliberais promoveram transformações no ensino superior. Nesse contexto, diversas instituições aderiram ao Programa de Apoio a Planos de Reestruturação e Expansão das Universidades Federais (REUNI). Isto, contudo, se deu sem as contrapartidas necessárias. O propósito da investigação, assim, foi compreender os impedimentos ao poder de agir de docentes que atuavam em um campus estruturado a partir do REUNI. Adotou-se o referencial da Clínica da Atividade. Participaram do estudo três docentes, duas mulheres e um homem, que atuavam em cursos de graduação e pós-graduação. Em uma perspectiva histórico-desenvolvimentista, foram realizadas entrevistas a partir de imagens que representavam elementos do contexto laboral. Utilizou-se análise construtivo-interpretativa. Dentre os resultados, viu-se intensificação do trabalho promovida pela atuação em cursos de pós-graduação e por atividades invisibilizadas; desprofissionalização associada ao desempenho de atividades alheias ao fazer docente; estrutura física inadequada; carga de trabalho elevada; pressões para o cumprimento de prazos; uso do espaço doméstico para o trabalho; e invasão do tempo livre. Os achados podem nortear ações de promoção da saúde docente e dar balizas para a discussão sobre as melhorias dos contextos de trabalho.

Neoliberal reforms promoted transformations in higher education. In this context, several institutions joined the Support Program for Restructuring and Expansion Plans of Federal Universities (REUNI). This happened without the necessary counterparts. The purpose of the investigation was to understand the impediments to the power to act of professors who worked in a campus structured from the REUNI. The reference of the Clinic of Activity was adopted. Three professors participated in the study, two women and one man, who worked in undergraduate and graduate courses. From a historical-developmental perspective, interviews were carried out based on images that represented elements of the work context. Constructive-interpretative analysis was used. Among the results, it was seen the intensification of work promoted by the performance in postgraduate courses and by invisible activities; de professionalization associated with the performance of activities outside the teaching profession; inadequate physical structure; high workload; pressure to meet deadlines; use of domestic space for work and invasion of free time. The findings can guide actions to promote teachers' health and provide guidelines for the discussion on improvements in work contexts.

Las reformas neoliberales impulsaron transformaciones en la educación superior. En ese contexto, varias instituciones se adhirieron al Programa de Apoyo a los Planes de Reestructuración y Expansión de las Universidades Federales (REUNI). Esto sucedió sin las contrapartes necesarias. La investigación tuvo como objetivo comprender los impedimentos al poder de actuar de los profesores que actuaban en un campus estructurado a partir de lo REUNI. Se adoptó la referencia de la Clínica de la Actividad. Participaron del estudio tres profesores, dos mujeres y un hombre, que actuaban en cursos de pregrado y posgrado. Desde una perspectiva histórica e de desarrollo, se realizaron entrevistas a partir de imágenes que representaban elementos del contexto de trabajo. Se utilizó el análisis constructivo-interpretativo. Entre los resultados, se vio la intensificación del trabajo promovida por la actuación en cursos de posgrado y por actividades invisibles; desprofesionalización asociada al desempeño de actividades ajenas a la docencia; estructura física inadecuada; alta carga de trabajo; presión para cumplir los plazos; uso del espacio doméstico para el trabajo e invasión del tiempo libre. Los hallazgos pueden orientar acciones para promover la salud de los docentes y proporcionar pautas para la discusión sobre mejoras en los contextos de trabajo.

A Framework for Human Corneal Endothelial Cell Culture and Preliminary Wound Model Experiments with a New Cell Tracking Approach.

Cell injection therapy is emerging as an alternative to treat corneal endothelial dysfunction (CED) and to avoid corneal scarring due to bullous keratopathy. However, establishing a standardized culture procedure that provides appropriate cell yield while retaining functional features remains a challenge. Here, we describe a detailed framework obtained from in vitro culture of human corneal endothelial cells (HCECs) and comparative in vivo experimental models for CED treatment with a new cell tracking approach. Two digestion methods were compared regarding HCEC morphology and adhesion. The effect of Y-27632 (ROCKi) supplementation on final cell yield was also assessed. Cell adhesion efficacy with two cell delivery systems (superparamagnetic embedding and cell suspension) was evaluated in an ex vivo human cornea model and in an in vivo rabbit CED model. The injection of supplemented culture medium or balanced salt solution (BSS) was used for the positive and negative controls, respectively. HCEC isolation with collagenase resulted in better morphology and adhesion of cultured HCEC when compared to EDTA. Y-27632 supplementation resulted in a 2.6-fold increase in final cell yield compared to the control. Ex vivo and in vivo adhesion with both cell delivery systems was confirmed by cell tracker fluorescence detection. Corneal edema and opacity improved in both animal groups treated with cultured HCEC. The corneas in the control groups remained opaque. Both HCEC delivery systems seemed comparable as treatments for CED and for the prevention of corneal scarring.

Correr atrás do vento: o trabalho dos mototaxistas de Sobral, Ceará / Chasing the wind: the work of motorcycle taxi drivers from Sobral, Ceará / Perseguir el viento: el trabajo de los mototaxis de Sobral, Ceará

A pesquisa teve como objetivo geral analisar a atividade e o ofício dos mototaxistas de Sobral, Ceará. O objetivo específico foi compreender: a) a atividade exercida e o conceito de real da atividade; b) as prescrições; c) as regras informais; e d) as relações profissionais. A investigação adotou o referencial teórico da Clínica da Atividade, e participaram oito mototaxistas. Inspiradas no método da Instrução ao Sósia, foram realizadas e gravadas entrevistas semiestruturadas. Seu áudio foi transcrito e o conteúdo passou por análise construtivo-interpretativa. Os resultados mostraram que a atividade era regulamentada pela prefeitura e dependia da manutenção dos instrumentos utilizados. O expediente era organizado por uma regra do coletivo de trabalho relacionada ao horário de funcionamento de escolas e universidades. A busca por passageiros variava, sendo possível ficar em pontos fixos ou circular pela cidade. As condições ambientais demandavam cuidados como o uso de protetor solar e hidratação. A autonomia da profissão permitia organizar o próprio expediente e o ganho mensal. Entretanto, houve queixas relativas à falta de direitos previdenciários e ao fato de que a flexibilidade geraria uma sobrecarga de trabalho que poderia trazer prejuízos à saúde. Riscos foram identificados em acidentes de trânsito e violência urbana. Os relatos acerca das relações com os colegas mostraram um ambiente amistoso, e as relações com os passageiros eram definidas por cada situação, abrangendo desde interações objetivas até conversas pessoais.(AU)

The research had as general objective to analyze the activity and the work of motorcycle taxi drivers in Sobral, Ceará. Specific objectives were to understand: (a) the activity performed and the real of activity; (b) the prescriptions; (c) informal rules and (d) professional relationships. The investigation adopted the theoretical framework of the Clinic of Activity. Eight motorcycle taxi drivers participated in the investigation. Inspired by the instruction to the double method, semi-structured interviews were conducted. The audio of the interviews was transcribed and underwent constructive-interpretive analysis. The results showed that the activity was regulated by the city and depended on the maintenance of the instruments used. The working hours were organized by a collective labor rule related to the opening hours of schools and universities. The looking-for for passengers also varied: it was possible to stay at fixed points or move around the city. Environmental conditions required care such as the use of sunscreen and hydration. The profession's autonomy made it possible to organize its own hours and monthly earnings. However, there were complaints regarding the lack of social security rights and that flexibility would create an overload of work that could harm health. Risks have been identified in traffic accidents and urban violence. Reports about relationships with colleagues showed a friendly atmosphere. Relations with passengers were defined by each situation, ranging from objective interactions to personal conversations.(AU)

La investigación tuvo como objetivo general analizar la actividad y el trabajo de los mototaxis en Sobral, Ceará. Los objetivos específicos eran comprender: (a) la actividad realizada y la real de la actividad; (b) las prescripciones; (c) reglas informales y (d) relaciones profesionales. La investigación adoptó el marco teórico de la Clínica de Actividad. En la investigación participaron ocho mototaxis. Inspirándose en el método de instrucción al doble, se realizaron entrevistas semiestructuradas. El audio de las entrevistas fue transcrito y sometido a un análisis constructivo-interpretativo. Los resultados mostraron que la actividad estaba regulada por la prefectura municipal y dependía del mantenimiento de los instrumentos utilizados. Los horarios de trabajo fueron organizados por una norma laboral colectiva relacionada con los horarios de apertura de escuelas y universidades. La búsqueda de pasajeros también variaba: era posible permanecer en puntos fijos o moverse por la ciudad. Las condiciones ambientales requerían cuidados como el uso de protector solar e hidratación. La autonomía de la profesión permitió organizar sus propias horas y ganancias mensuales. Sin embargo, hubo quejas sobre la falta de derechos de seguridad social y que la flexibilidad crearía una sobrecarga de trabajo que podría dañar la salud. Se han identificado riesgos en accidentes de tráfico y violencia urbana. Los informes sobre las relaciones con los colegas mostraron un ambiente agradable. Las relaciones con los pasajeros se definieron por cada situación, desde interacciones objetivas hasta conversaciones personales.(AU)

Lacticaseibacillus casei decrease long-chain fatty acids and most substances in an experimental model of intestinal mucositis

Purpose: To evaluate the long-chain fatty acid and major compounds levels in the feces after prophylactic oral use of Lacticaseibacillus casei in an experimental model of intestinal mucositis. Methods: Fifteen Swiss mice were randomly divided into three groups (n=5/group): The negative or positive control groups (n = 5) received saline orally for 18 days and an the intraperitoneal (i.p.) of saline or 5 Fluorouracil (450 mg/kg) in 15th day, respectability. L. casei group received oral concentration of L. casei (1x109 CFU/mL) for 18 days, the i.p. injection of 5-fluorouracil (450 mg/kg) in 15th days. Tissue samples from colon and each small intestine segment were collected for histopathological analysis. Stool samples were collected. Fecal composition of long-chain fatty acids and sterols were analysed by gas chromatography-mass spectrometry on the 15th and the 18th day. Results: The mucosa layer of all small intestine segments of animals from L. casei showed well preserved epithelium and glands, without necrosis signs, but Goblet cells number decreased. Several long-chain fatty acids and sterols have been identified before and after in the groups. L. casei administration after 5-FU treatment reduced concentrations of linoleic acid (18:2) (p < 0.001) and oleic acid (18:1) (p < 0.001) in feces. Conclusions: L. casei prevented the mucosal damage associated with 5-FU-induced intestinal mucositis reduced long-chain fatty acid levels in the feces.

Effects of school violence and work context on teachers' health / Efeitos da violência escolar e do contexto de trabalho na saúde de docentes / Efectos de la violencia escolar y del contexto laboral en la salud de los profesores

The presented study aimed to understand how school violence and work context affect teachers' health. The study included 744 teachers of public schools. The instruments used for data collection were: Victimization Questionnaire, Work Context Assessment Scale, and Work-Related Harm Scale. Multinomial logistic regression analyses were performed. The results indicate that a high percentage of the participants considered the psychological and social harms as bearable. More than a third of the teachers evaluated physical harm more negatively. Gender and employment status were associated with physical harm. Physical aggression affected psychological harm. Verbal harassment and work organization were predictors of all types of harm. We concluded that models of work structuring, management practices, and being a victim of verbal harassment have a central role in teachers' health

O presente estudo visou compreender como a violência escolar e o contexto de trabalho repercutem sobre a saúde de professores. Participaram da pesquisa 744 docentes de escolas públicas. Os instrumentos utilizados foram: Questionário de Vitimização, Escala de Avaliação do Contexto de Trabalho e Escala de Danos Relacionados ao Trabalho. Foram realizadas análises de regressão logística multinomial. Os resultados indicaram que um percentual alto de participantes considerou os danos psicológicos e sociais como suportáveis. Mais de um terço dos professores avaliaram os danos físicos de forma mais negativa. Gênero e vínculo empregatício se mostraram associados aos danos físicos. As agressões físicas incidiram sobre os danos psicológicos. Assédio verbal e organização do trabalho foram preditores de todos os tipos de danos. Conclui-se que os modelos de estruturação do trabalho, as práticas gerenciais e o assédio verbal desempenham um papel central para a saúde docente

La encuesta presentada tuvo como objetivo comprender cómo la violencia escolar y el contexto laboral impactan en la salud de los docentes. Participaron del estudio 744 docentes de escuelas públicas. Los instrumentos utilizados fueron: Cuestionario de Victimización, Escala de Evaluación del Contexto Laboral y Escala de Daños Relacionados con el Trabajo. Se realizaron análisis de regresión logística multinomial. Los resultados indicaron que un alto porcentaje de los participantes consideraba soportables los daños psicológicos y sociales. Más de un tercio de los docentes evaluó los daños físicos de manera más negativa. El género y la situación laboral se asociaron con los daños físicos. Las agresiones físicas afectaron a los daños psicológicos. El acoso verbal y la organización del trabajo fueron predictores de todos los daños. Se concluye que los modelos de estructuración del trabajo, prácticas gerenciales y acoso verbal juegan un papel central en la salud docente

Intensificação laboral e gênero profissional de professoras da educação infantil / Intensification of labor and professional gender of female teachers of early childhood education

As reformas neoliberais levaram à intensificação do trabalho, dada a exigência de maiores e melhores resultados em contextos laborais cujas condições eram insuficientes para realizá-lo. Este artigo visa compreender, a partir do referencial teórico da Clínica da Atividade, a relação entre intensificação do trabalho docente e gênero profissional. Trata-se de um estudo de caso derivado de uma intervenção com seis professoras da Educação Infantil que utilizou o método de instrução ao sósia. Os registros de áudio produzidos ao longo das instruções ao sósia foram transcritos e submetidos à análise construtivo-interpretativa. O número de alunos por sala, fator inerente à organização do trabalho, mostrou-se promotor dessa intensificação, uma vez que exigia mais dos profissionais, sem a contrapartida necessária das condições de trabalho. Nesse cenário, ocorreu a construção coletiva do que era chamado de "domínio de sala", norma de proceder não oficializada relativa às habilidades necessárias à atuação docente para lidar com a intensificação laboral. Desenvolveu-se, assim, uma cultura profissional que orientou a ação das docentes, de modo a viabilizar a atividade e preservar o poder de agir

Neoliberal reforms have led to the intensification of work, given the demand for greater and better results in labor contexts whose conditions were insufficient to realize it. This article aims to understand based on the theoretical framework of the Clinic of Activity the relationship between intensification of the teaching work and professional genre. This is a case study derived from an intervention with six children's education teachers that used the method of instruction to the look-alike. The audio recordings produced during the instruction to the look-alike were transcribed and submitted to constructive-interpretative analysis. The number of students per classroom, a factor inherent to the work organization, proved to be a promoter of this intensification, since it demanded more from professionals, without the necessary counterpart of working conditions. In this scenario there was a collective construction of what was called "classroom mastery," an unofficial rule of thumb related to the necessary skills for teaching to deal with work intensification. Thus, a professional culture was developed that guided the teachers' action to make the activity viable and preserve the power to act

Fertility decision-making during the Zika virus epidemic in Brazil: Where is the decision?

BACKGROUND: In 2016, a Public Health Emergency of International Concern (PHEIC) was declared in response to the rise of microcephaly cases among newborns in Northeastern Brazil. A common reactionary measure by public health authorities was to recommend women postpone pregnancy to avoid the possible perinatal transmission of Zika virus (ZIKV). METHODS: The purpose of this study was to assess how women in Fortaleza, Brazil conceptualize pregnancy; experience facilitators and barriers to pregnancy avoidance; perceive the authorities' recommendation to postpone pregnancy due to the ZIKV outbreak; and recall their experiences during the ZIKV epidemic. Qualitative methods, specifically a Rapid Anthropological Assessment (RAA), were utilized in this study. Data collection included semi-structured interviews, triangulated with observations and informal interviews with community members. RESULTS: The sample included 35 women (18-39 years old) who exclusively utilized the national public health care system. Findings indicated that all participants perceived the ZIKV pregnancy-postponement recommendation to be counter-cultural to Brazilian social norms. Overall women's self-perceived agency to prevent pregnancy was low due to social expectations and lack of trust for contraceptives. ZIKV prevention was not seen as a reason to utilize contraceptives. Interestingly, only women who self-perceived as more affluent were willing to attempt pregnancy prevention for educational, occupational, or financial opportunity. CONCLUSION: Pregnancy postponement as a response to a ZIKV epidemic ignores gaps in reproductive agency and defies social norms, making it unrealistic and counter-cultural. Future ZIKV health recommendations must be culturally aligned with the population, and address barriers and motivators for family planning.

Effects of Supplementation with Probiotics in Experimental Models of Alzheimer's Disease: A Systematic Review of Animal Experiments.

BACKGROUND: Conditions along the brain-gut-microbiota (BGM) axis can significantly contribute to the pathogenesis of Alzheimer's disease (AD). Evidence from animal studies indicates a role of probiotics in regulating mood, cognition, and stress response via the BGM axis. However, the effect of probiotics on AD needs to be better clarified in preclinical and clinical studies. METHODS: We prepared this systematic review according to PRISMA. PubMed, Web of Science, Embase, and Virtual Health Library (VHL) were searched for original articles concerning the effects of probiotics in experimental AD. RESULTS: Results were presented as a narrative synthesis according to the Synthesis Without Metaanalysis (SWiM) Guideline. Seventeen studies met the inclusion criteria. The results showed significant effects in the experimental models of AD treated with probiotics alone or in mixture due to expressive improvements in cognitive tests. CONCLUSION: Furthermore, in most of the included studies, it was possible to observe a reduction in inflammatory processes, an increase in the concentration of peptide hormones, insulin homeostasis in the brain, increased antioxidant enzymes, and a decrease in beta-amyloid deposition and tau hyperphosphorylation. Supplementation of probiotics seems to improve performance in cognitive tests and increase the concentration of substances capable of delaying the neurodegenerative process of AD in experimental models.

Surgery remember@: an innovation to reduce surgical cancellations.

The use of mobile phones has dramatically increased all over the world. Such revolution in the communication amongst individuals has a great impact in patient care, supporting their self-management and promoting shared responsibility with health services. Given that improved communication facilitates compliance with scheduled procedures and reduces surgical cancellations, the current work aims to develop a communication tool named Surgery Remember@ to mitigate surgical suspensions due to patient absenteeism. The present article is a study of technological production divided into four chapters: literature review; analysis of the hospital administrative profile; software development; and process mapping for software implementation. Taking into account that in the last three years the problem of absenteeism was the main cause of institutional surgical cancellations; the development of Surgery Remember@ endeavours to reduce surgery cancellations, improving efficiency and reducing costs. It is known that sending messages three days before the surgical procedure makes it possible to replace patients in the event of cancellations, optimizing the human and material resources in the operating room. The confirmation of the pre-aesthetic consultation is also positive, for it allows the verification of perioperative assistance improvement. Hence, besides being viable and easy to implement, the software developed allows the addition of other features based on user requirements, proving to be an asset to reduce surgery cancellations.

Leiomyoma of the Vulva: Case Report.

BACKGROUND: Leiomyoma of the vulva is rare, accounting for only 0.03% of all gynecological tumors, and it is seldom seen in teenagers. CASE: We describe a case of vulvar leiomyoma in a 14-year-old girl who presented a 10 × 10 cm solid tumor in the right hemivulva without other complaints. SUMMARY AND CONCLUSION: Differential diagnosis includes Bartholin cysts, abscesses, fibromas, and other solid lesions. Although rare, vaginal leiomyoma must be remembered and included as a differential diagnosis for solid lesions in the vagina.

Evaluation of efficacy of new lens nucleus fragmenting forceps in cataract surgery / Avaliação da eficácia de uma nova pinça fragmentadora de núcleo do cristalino para cirurgia de catarata

ABSTRACT Objective: To evaluate the efficacy of low-cost nucleus fragmenting forceps designed to reduce the use of ultrasound during phacoemulsification. Methods: A total of 60 pig eyes enucleated 10 hours before were placed in the microwave oven, at maximum power for 10 seconds, to form cataracts with hardness comparable to a grade IV nucleus in the Lens Opacities Classification System III. Cataract extraction was performed using the Centurion® phacoemulsifier (Alcon Laboratories, Geneve, Switzerland) and Leica M620 microscope. All eyes were submitted to the pre-chop technique dividing the nucleus into four parts. After the pre-chop, the quadrants in 30 eyes were phacoemulsified with the torsional mode and were fragmented in the remaining 30 eyes after the pre-chop was with the fragmentation forceps before torsional mode phacoemulsification. The device was calibrated for all eyes by applying the following parameters: 40% linear torsional phacoemulsification; intraocular pressure of 65 mmHg; the linear vacuum of 600 mmHg; aspiration flow of 40 ccs/minute. After each procedure, the following was recorded: cumulative dissipated energy; equivalent average torsional amplitude; equivalent average ultrasonic power; estimated aspirated fluid; ultrasound total time; and total aspiration time. Statistical analysis was performed using the Kruskal-Wallis test and the IBM Statistical Package for Social Sciences. The p-value <0.05 was considered statistically significant. Results: There was a statistically significant reduction favoring the use of the nucleus fragmenting forceps in all parameters, except for the average torsional amplitude. Conclusion: The use of the nucleus fragmenting forceps contributed to improving the efficacy of torsional phacoemulsification in enucleated pig eyes.

RESUMO Objetivo: Avaliar a eficácia de uma pinça fragmentadora de núcleo, de baixo custo, desenvolvida para reduzir o uso de ultrassom durante a emulsificação do núcleo. Métodos: Sessenta olhos de porco com 10 horas de enucleação foram colocados no forno de microondas, em potência máxima por 10 segundos, para a formação de catarata com dureza comparável à de um núcleo grau IV na Lens Opacities Classification System III. A extração da catarata foi realizada com o facoemulsificador Centurion® (Alcon Laboratories, Genebra, Suíça) e microscópio Leica M620. Todos os olhos foram submetidos a técnica de pre-chop, dividindo o núcleo em quatro partes. Em 30 olhos, após o pre-chop, foi feita a facoemulsificação dos quadrantes com o modo torsional e, nos outros 30 olhos, após o pre-chop, cada quadrante foi fragmentado com a pinça antes da facoemulsificação com o modo torsional. O aparelho foi calibrado para todos os olhos com os seguintes parâmetros: faco torsional linear 40%; pressão intraocular 65 mmHg; vácuo linear 600mmHg e fluxo de aspiração 40cc/minuto. Após cada procedimento, verificaram-se energia dissipada acumulada; média da amplitude do faco torsional; média equivalente do poder ultrassônico; líquido aspirado estimado; tempo total de ultrassom e tempo total de aspiração. A análise estatística foi realizada utilizando o teste de Kruskal-Wallis com o IBM Statistical Package for Social Sciences. Valor de p<0,05 foi considerado estatisticamente significante. Resultados: Houve redução estatisticamente significante em favor do uso da pinça fragmentadora de núcleo em todos os parâmetros, menos na média de amplitude do faco torsional. Conclusão: O uso da pinça fragmentadora de núcleo contribuiu para melhorar a eficácia do faco torsional em olhos de porco enucleados.