A hybrid catalyst-triggered cascade reaction for cholesterol detection using a smartphone-based miniature fluorescent apparatus.

A new hybrid biological-chemical catalyst, magnetic nanoparticles functionalized with cholesterol oxidase (Fe3O4/APTES/ChOx), was developed for cholesterol detection. In the presence of cholesterol, the enzyme produced H2O2, which facilitated the generation of fluorescent molecules from the fluorogenic substrate with the assistance of Fe3O4 nanoparticles. A smartphone camera with a miniature fluorescent apparatus was used to assess fluorescence emission. Then, a smartphone application was employed to translate the fluorescence intensity to the red, green, and blue (RGB) domain. The developed approach achieved excellent selectivity and acceptable performances while supporting an onsite analysis approach. The practical operational range spanned from 5 to 100 nM, with a detection limit of 0.85 nM. Fe3O4/APTES/ChOx was applied for up to four replicates of reuse and demonstrated stability for at least 30 days. The applicability of the method was evaluated in milk samples, and the results were in accordance with the reference method.

Smartphone-based portable fluorescence sensor with gold nanoparticle mediation for selective detection of nitrite ions.

A simple, portable device for the detection of NO2- via a fluorescence method was developed. The proposed device consisted of a dark box containing a blue LED as a low-power excitation light source and a smartphone with a mobile application for RGB analysis as a light detector. Detection was mediated by using synthesized cetyltrimethylammonium bromide-stabilized gold nanoparticles (CTAB-AuNPs). The CTAB-AuNPs were etched with NO2- to yield Au3+, which catalyzes the oxidation of o-phenylenediamine (OPD) in the presence of H2O2 to generate 2,3-diaminophenazine (DAP). Triton X-100 (TX-100) micelles were introduced to improve the DAP fluorescence emission. The fluorescence intensity of DAP was recorded by the smartphone in terms of RGB intensity, which was correlated with the NO2- concentration. This method provided a wide linear working concentration range (0.5-100 µM), a limit of detection of 0.17 µM and excellent selectivity for NO2- over other anions.

A sensitive and selective on-line amperometric sulfite biosensor using sulfite oxidase immobilized on a magnetite-gold-folate nanocomposite modified carbon-paste electrode.

We describe a novel amperometric sulfite biosensor, comprising a carbon-paste electrode (Fe3O4@Au-Cys-FA/CPE) modified with immobilized sulfite oxidase (SOx) on a gold-coated magnetite nanoparticle core, encased within a conjugated folic acid (FA) cysteine (Cys) shell. The biosensor electrode was fabricated using a polydimethylsiloxane (PDMS) and mineral oil mixture as binder, which also enhances the physical stability and sensitivity of the electrode. The developed biosensor displays good electrocatalytic activity toward oxidation of H2O2, which occurs by an enzymatic reaction between SOx and sulfite. The Fe3O4@Au-Cys-FA electrode exhibits good electrocatalytic activity, and has good retention of chemisorbed SOx on the electrode because of its large surface area. Sulfite was quantified using amperometric measurements from the Fe3O4@Au-Cys-FA/CPE biosensor, and using an in-house assembled flow cell at +0.35V (vs. Ag/AgCl), with a phosphate buffer carrier (0.10M, pH 7.0) at a flow rate of 0.8mLmin(-1). The system detects sulfite over the range 0.1-200mgL(-1) (r(2)=0.998), with a detection limit of 10µgL(-1) (3σ of blank). The system exhibits acceptable precision (%R.S.D.=3.1%), rapid sample throughput (109samplesh(-1)), and good stability (2w). The developed biosensor shows satisfactory tolerance to potential interferences, such as sugars, anions, ascorbic acid, and ethanol. We applied the developed method to the determination of sulfite content in wines and pickled food extracts, and our results are in good agreement with those obtained by the standard iodometric method.