Calcium-modified clinoptilolite as a recovery medium of phosphate and potassium from anaerobically digested olive mill wastewater.

Olive mill wastewater (OMW) is characterized as a high-strength effluent due to the high organic load, low biodegradability, and presence of phytotoxic compounds. Most of the OMW treatment methods proposed, including adsorption, focus mainly on the reduction of chemical oxygen demand and recovery of polyphenols. Adsorption studies aiming at nutrient removal from OMW are very limited. In the present work, Ca(OH)2-treated zeolite (CaT-Z) in a granular form was used for simultaneous recovery of phosphate (PO43-) and potassium (K+) ions from two samples of anaerobically digested OMW. Nutrient adsorption was investigated as a function of contact time, pH and dilution of OMW with deionized water. The lower removal efficiency of phosphorus (P) by CaT-Z was observed at higher dilution ratios consisted of 3.125-6.25% OMW-1 and 5% OMW-2. The maximum P removal was 73.9% in 25% OMW-1 and 85.9% in 10% OMW-2. Potassium removal, as the predominant cation of OMW samples, increased from 17.3 to 46.1% in OMW-1 and from 15.1 to 57.7% in OMW-2 with increasing dilution. The maximum experimental adsorption capacities were 15.8 mg K and 2.14 mg P per gram of CaT-Z. Five sequential treatments of 50% OMW-2 with fresh CaT-Z at each stage ensured a cumulative removal of 87.5% for P and 74.9% for K. Adsorption kinetics were faster for K than for P. The plant-available P was found to be the predominant fraction on the loaded CaT-Z. Electron Probe Micro-analysis confirmed the enhanced content of K and P on the loaded CaT-Z, whereas X-ray mapping revealed the co-distribution of Ca and P. This study demonstrates the potential usage of CaT-Z as an immobilization medium of P and K from anaerobically treated OMW.

Changes in sugar metabolism associated to stem bark thickening partially assist young tissues of Eriobotrya japonica seedlings under boron stress.

Boron (B) toxicity frequently affects plant performances and productivity, especially in arid and semi-arid environments. In this experiment, loquat seedlings were subjected to 25 µM (control) or 400 µM B (B excess) to test the hypothesis that (i) B alters sugar/polyol metabolism in polyol-producing tree species as loquat and (ii) changes of leaf and stem anatomy assist young tissues against toxic effect of B. Gas exchange was monitored from the beginning of the experiment (FBE) till one week after the first visible symptoms of B toxicity appeared in the upper part of the stems (147 d FBE). At 147 FBE, plant biometric parameters and pattern of B accumulation, leaf and stem anatomy, chlorophyll a fluorescence kinetics as well as biochemical measurements were assessed in top (asymptomatic) leaves and upper stem bark. Boron accumulated principally (in the row) in top leaves > top bark > top wood in B-stressed plants, but no changes in allocation pattern were found between controls and B-stressed plants. Excess B promoted the increase in the spongy layer of top leaves and caused the development of cork and numerous collenchyma cells with increased cell wall thickness. This mechanism, which has never been described before, can be considered an attempt to store excessive B in tissues where B ions are less harmful. The accumulation of sorbitol (B-complexing polyol) in top leaves and stem bark can be considered as a further attempt to detoxify B excess. However, B toxicity drastically affects the photosynthetic rate of top leaves, mainly due to non-stomatal limitations, i.e., reduction of ambient CO2 use efficiency and of photosystem II (PSII) efficiency, modification of the partitioning excess energy dissipation in PSII, thus leading to an increased level of lipid peroxidation. Our results suggest that changes in sugar metabolism associated with leaf and stem bark thickening partially assist (but not totally preserve) young tissues of loquat plants under B stress.