A new genus and new species of tangle-veined flies of the tribe Hirmoneurini (Nemestrinidae), Bernardia xenomorpha gen. et sp. nov., is described from the highland savannas of Brazil. This represents the first record of this tribe from the Southern Hemisphere. The new taxon is compared with the related genera and species of Hirmoneurinae. Bernardia xenomorpha gen. et sp. nov. fits perfectly in the diagnosis of the subfamily, especially by the reduction of the hypandrium and by having the proboscis reduced and, in the tribe Hirmoneurini, by having the frons projected forward and covered by dense pilosity. Bernardia xenomorpha gen. et sp. nov. is distinguished from the other species of Hirmoneurinae, mainly by the length of the proboscis, which reaches the first pair of coxae, while in the other species the proboscis does not exceed the length of the head and, by the U-shaped genital fork, with simple (not bifurcate) arms. An identification key for the American subfamilies, tribes, and genera of Nemestrinidae is also included.
Diptera , Euphorbiaceae , Animals , Animal Distribution , Body Size , Organ Size
ABSTRACT The SISBIOTA-BRASIL was a three-year multimillion-dollar research program of the Brazilian government to document plants and animals in endangered/understudied areas and biomes in Brazil. Distributional patterns and the historical events that generated them are extensively unknown regarding Brazilian fauna and flora. This deficiency hinders the development of conservation policies and the understanding of evolutionary processes. Conservation decisions depend on precise knowledge of the taxonomy and geographic distribution of species. Given such a premise, we proposed to research the diversity of Diptera of the Brazilian western arc of Amazon, Cerrado, and Pantanal in the states of Mato Grosso, Mato Grosso do Sul, and Rondônia. Three important biomes of the South American continent characterize these Brazilian states: Amazon forest, Cerrado (Brazilian Savannah), and Pantanal. Besides their ecological relevance, these biomes historically lack intensive entomological surveys. Therefore, they are much underrepresented in the Brazilian natural history collections and in the scientific literature, which is further aggravated by the fact that these areas are being exponentially and rapidly converted to commercial lands. Our project involved over 90 collaborators from 24 different Brazilian institutions and one from Colombia among researchers, postdocs, graduate and undergraduate students, and technicians. We processed and analyzed nearly 300,000 specimens from ~60 families of Diptera collected with a large variety of methods in the sampled areas. Here, we provide a detailed overview of the genera and species diversity of 41 families treated. Our results point to a total of 2,130 species and 514 genera compiled and identified for the three states altogether, with an increase of 41% and 29% in the numbers of species and genera known for the three states combined, respectively. Overall, the 10 most species-rich families were Tachinidae, Cecidomyiidae, Tabanidae, Psychodidae, Sarcophagidae, Stratiomyidae, Bombyliidae, Syrphidae, Tephritidae, and Asilidae. The 10 most diverse in the number of genera were Tachinidae, Stratiomyidae, Asilidae, Mycetophilidae, Syrphidae, Tabanidae, Muscidae, Dolichopodidae, Sarcophagidae, and Chloropidae. So far, 111 scientific papers were published regarding taxonomic, phylogenetic, and biogeographical aspects of the studied families, with the description of 101 new species and three new genera. We expect that additional publications will result from this investigation because several specimens are now curated and being researched by specialists.
ABSTRACT The complex taxonomy of Albardia furcata van der Weele is totally related to the unique characters of the adults, which hinder a morphological comparison with other owlfly genera. The behavior and life history of the immature stages of A. furcata are known, but the larval morphology remains undescribed. Herein, we present a morphological description of the A. furcata larva, the most iconic Neotropical owlfly (Myrmeleontidae: Ascalaphinae), a Brazilian species endemic to Cerrado, Caatinga, and Atlantic Forest biomes. The morphological description of the larva was based on three third instar larvae collected in areas of Cerrado and reared at laboratory conditions. We provide high-resolution images from the life cycle, including immature and adults, as well as an updated distributional map. Comments are done on the similarities and differences of the larva regarding other owlflies species and also on biogeography in Neotropical region.
Ants are one of the first insects to find an exposed cadaver and can be present during all stages of decomposition. Although these organisms are not commonly used in postmortem interval estimates, they are to be taken into account on criminal investigations involving human corpses, since they can leave bite marks that can be mistaken for antemortem or perimortem injuries, which could be misleading when ascertaining the occurrence of abuse or physical altercation during a crime. A few studies report the action of ants on human cadavers and even though leaf-cutting ants of the genus Atta are frequently encountered in succession studies that use animal carcasses, there are no records of these fungus-growing species on human corpses. Atta is a genus restricted to the New World, ranging from northern Argentina to southern United States and acts as one of the most conspicuous neotropical herbivores. In this study, we report three cases of violent death that illustrate the impact of ants, especially those of the genus Atta, in a forensic setting. We compare the patterns displayed by postmortem bite injuries caused by leaf-cutter ants and other common species with less robust mandibles. We also present the capability of Atta ants to create artifacts by cutting victim's clothes in a crime scene, contributing to the knowledge of ant-mediated confounding factors in crime scene investigation.
Ants , Artifacts , Bites and Stings/pathology , Feeding Behavior , Postmortem Changes , Adult , Animals , Cadaver , Child , Clothing , Female , Forensic Entomology , Humans , Male
Five Rachicerus species are recorded in Brazil: Rachicerus lanei Carrera, 1940, R. oliverioi Carrera, 1940, R. lopesi Carrera, 1940, R. marcusi Carrera, 1940 and, R. shannoni Carrera, 1945. Here I describe a new species Rachicerus carrerai sp. nov. based on three male specimens from Brazil (Amazonia and Mato Grosso do Sul) and Colombia (Chocó). The new species is distinguished from the other known species by the antenna with bipectinate flagellomeres, a character only known from fossils. The conditions of the Messias Carrera's type specimens are discussed and a key for the Brazilian species of Rachicerus is provided.
Bivalvia , Diptera , Animals , Brazil , Colombia , Fossils , Male
Oxelytrum discicolle is a carrion beetle commonly found in the Neotropical region, mainly associated with cadavers and carcasses. Information on behavior related to the likelihood of cadaver colonization is scarce. We performed two field experiments in peri-urban forest in Brazil in order to strengthen our knowledge on O. discicolle behavior. In the first experiment, we assessed the preference for piglet carcasses at different stages of decomposition offered simultaneously in two seasons, dry and rainy, while in the second experiment we investigated the diel activity of adults. A total of 493 adults were collected in the experiments. A significantly higher abundance was detected in the rainy season. There was a marked preference for carcasses at advanced stages. Adults exhibited a typical nocturnal behavior, the number trapped at night was 17 times greater than those collected during the day. Oxelytrum discicolle is nocturnal species that prefers carcasses in advanced stage of decomposition.
Coleoptera/physiology , Feeding Behavior/physiology , Postmortem Changes , Swine , Animals , Brazil , Coleoptera/anatomy & histology , Entomology , Forensic Pathology , Humans , Humidity , Models, Animal , Rain , Seasons , Temperature , Time Factors
ABSTRACT: Natural biological control is one of the major causes responsible for reduction of pest population in agricultural ecosystem. However, natural biological control importance is usually minimized by not being estimated. This study reports the occurrence of Campoletis sonorensis (Cameron, 1886) (Hymenoptera: Ichneumonidae), Archytas marmoratus (Townsend, 1915) and Archytas incertus (Macquart, 1851) (Diptera: Tachinidae) associated with Helicoverpa armigera (Hübner, 1809) (Lepidoptera: Noctuidae). The rate of larval parasitism was up to 41% in structured refuge areas of cotton, without chemical control with insecticides. This study strengthens our knowledge relating to parasitoids associated with natural control of H. armigera in the American continent. In addition, it documents the rule that structured refuge areas could play as a source of natural enemies, besides their contribution to production of non-selective populations to Bt technology.
RESUMO: O controle biológico natural é um dos principais componentes responsáveis pela redução populacional de pragas em agroecossistemas. Entretanto, a importância do controle biológico natural, usualmente, é minimizada devido não ser estimada. Este estudo documenta a ocorrência de Campoletis sonorensis (Cameron, 1886) (Hymenoptera: Ichneumonidae), Archytas marmoratus (Townsend, 1915) e Archytas incertus (Macquart, 1851) (Diptera: Tachinidae) associados a Helicoverpa armigera (Hübner, 1809) (Lepidoptera: Noctuidae). A taxa de parasitismo larval foi superior a 41% em áreas de refúgio estruturado de algodão, sem uso de inseticidas químicos. Este estudo fortalece o conhecimento relacionado com os parasitoides associados ao controle natural de H. armigera no Continente Americano. Adicionalmente, documenta o efeito que áreas de refúgio estruturado podem desempenhar como fonte de inimigos naturais, além da sua contribuição para produzir insetos não expostos à tecnologia Bt.
The puparium of Hermetia pulchra is described, based on a single specimen reared in laboratory under controlled conditions until the emergence of an adult female. The puparium of H. pulchra was compared with puparium of H. illucens and some differential features were indicated. A checklist of the Brazilian species of the genus Hermetia is included, with new distributional records for H. pulchra in the Brazilian States of Bahia and Distrito Federal. Hermetia sphecodes is recorded for the first time for Brazil (State of Amazonas).
Diptera/anatomy & histology , Diptera/classification , Animal Distribution , Animal Structures/anatomy & histology , Animal Structures/growth & development , Animals , Body Size , Brazil , Diptera/growth & development , Ecosystem , Female , Male , Organ Size
ABSTRACT The intra-puparial development of the blowflies Cochliomyia macellaria (n = 310) and Lucilia cuprina (n = 470), was studied under controlled conditions in laboratory. The 3rd instar larvae were reared until they stopped feeding, and the pre-pupae were separated according to the size in larval length and degree of pigmentation and of the cuticle. We observe a set of five continuous events or phases: (1) pupariation, (2) larva-pupa apolysis, (3) cryptocephalic pupa, (4) phanerocephalic pupa and (5) pharate adult. The total time of the intra-puparial development, larva-pupa apolysis to pharate adult, lasted for 120 h (5 days) to C. macellaria and 210 h (8.75 days) to L. cuprina.
The puparium of Chorophthalmyia brevicornis is described for the first time, based on five puparia. Larvae of the species were reared in the laboratory under semi-controlled conditions until the emergence of the adults. In addition, to the description of the puparium we provide a redescription of the imagoes of C. brevicornis.
Diptera/anatomy & histology , Diptera/classification , Animals , Brazil , Female , Larva , Male , Pupa
Little is known regarding the scavenger fauna associated with buried human corpses, particularly in clandestine burials. We report the presence of 20 shells of the terrestrial snail Allopeas micra, within hollow bones of human remains buried for 5 years, during the process of collecting DNA material. The fact that a large number of shells of A. micra had been found in the corpse and in the crime scene supports the assumption that there was no attempt to remove the corpse from the area where the crime occurred. Despite this, our observations cannot be used to estimate the postmortem interval because there is no precise knowledge about the development of this species. This is the first record of a terrestrial snail associated with a human corpse and its role in this forensic medicine case.
Bone and Bones/pathology , Burial , Snails , Adult , Animals , Brazil , Forensic Anthropology , Humans , Male , Microscopy, Electron, Scanning
The intra-puparial development of the black soldier-fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), was studied based on 125 pupae under controlled conditions in laboratory. The 6(th) instar larvae were reared until they stopped feeding, and the prepupae were separated according to the reduction in larval length and degree of pigmentation and sclerotization of the cuticle. The pupal stage lasted eight days (192 hours). The process of pupation (larva/pupa apolysis) occurred in the first six hours, extroversion of the head and thoracic appendages took place between the ninth and 21(st) hours, and the pharate appeared 21 hours after completing pupation. After pupariation, four morphological phases of intra-puparial development were observed and described.
Diptera/growth & development , Animals , Pupa/growth & development , Respiratory System/growth & development
The stilt-legged fly genus Micropeza contains 82 valid species. Currently 10 species are recorded in Brazil. Here we describe a new species, M. dactyloptera sp. nov., based on 108 specimens (55 males and 53 females) from the States of Goiás, Paraná, and Distrito Federal, Brazil.
Diptera/classification , Animal Distribution , Animal Structures/anatomy & histology , Animal Structures/growth & development , Animals , Body Size , Brazil , Diptera/anatomy & histology , Diptera/growth & development , Female , Male
Origin of samples of Cannabis sativa through insect fragments associated with compacted hemp drug in South America. Insects associated with a seizure of Cannabis sativa L. may indicate the origin of the illicit drug. Nevertheless, no work regarding this subject has been previously published for South America. In the present investigation, seven kilograms of vegetal material (C. sativa) were inspected for insect fragments. Three species were identified and used to test the origin of the seizure of cannabis plant material: Euschistus heros (Fabricius, 1794), Thyanta perditor (Fabricius, 1794) (Heteroptera, Pentatomidae), and Cephalotes pusillus (Klug, 1824) (Hymenoptera, Formicidae). These insect species restricted the geographic origin of the drug to the Neotropical region, and their distribution patterns showed an overlap of the State of Mato Grosso (Brazil), Argentina, and Paraguay. Based on this information, two of the three major C. sativa growing areas in South America were excluded: (1) the Colombian territory and (2) northeastern Brazil.
A new species of Giovanella Bonatto (Diptera, Calliphoridae, Mesembrinellinae) from Colombia. The Mesembrinellinae are exclusively Neotropical, with 33 nominal species distributed in nine genera. Only the genera Mesembrinella, Eumesembrinella, Huascaromusca and Laneella had until now been recorded in Colombia. In this work we present a new species of Giovanella Bonatto, 2005, genus with only one species, G. bolivar Bonatto, differing from the new species G. carvalhoi sp. nov., in the characters: legs entirely dark chestnut; thorax with dorsocentrals setae 1:2; abdomen with T5 with discal bristles poorly differentiated; T6 symmetric and paraphallus not extended and with denticules. Giovanella carvalhoi sp. nov. is from Cordillera Oriental, from the Departments of Caquetá (Amazonian foothills) and Santander, Colombia, collected between 22002400 m., and associated with decomposing organic matter. A key for the identification of males and females of the two species of Giovanella, illustrations of the genitalia and photographs of male and female of new species are also presented.
Sarcophagidae and Calliphoridae related to Rhinella schneideri (Anura, Bufonidae), Bothrops moojeni (Reptilia, Serpentes) and Mabuya frenata (Reptilia, Lacertilia) carcasses in Brasília, Brazil. This paper presents a list of necrophagous insects associated with small size carrions of two reptiles and one amphibian, found in areas of riparian forests and Cerrado sensu stricto physiognomies in a Conservation Unit located in Brasilia, Distrito Federal. We found seven species of insects related to these carcasses, being five Sarcophagidae, one Calliphoridae and one Braconidae parasitoid wasp. Lucilia eximia and Peckia (Pattonella) intermutans were the most abundant species in the study, corroborating with other studies that suggests that these species have specializations for colonization of small size animal carcasses.
Sarcophagidae e Calliphoridae associados às carcaças de Rhinella schneideri (Anura, Bufonidae), Bothrops moojeni (Reptilia, Serpentes) e Mabuya frenata (Reptilia, Lacertilia) em Brasília, Distrito Federal, Brasil. Este trabalho apresenta uma lista de insetos decompositores associados a carcaças de pequeno porte de dois répteis e de um anfíbio, encontrados em áreas de matas de galeria e de cerrado sensu stricto em unidades de conservação do Distrito Federal. Foram encontradas sete espécies de insetos associados a essas carcaças, sendo cinco sarcofagídeos, um califorídeo e uma vespa parasitóide Braconidae. Lucilia eximia e Peckia (Pattonella) intermutans foram as espécies mais abundantes, corroborando com outros estudos que sugerem que estas espécies apresentam especializações para a colonização de carcaças menores.
Intra-puparial development of the females of Chrysomya albiceps (Wiedemann) (Diptera, Calliphoridae). The chronology and morphological changes that take place during intra-puparial development of Chrysomya albiceps is described based on 254 specimens reared in the laboratory. Larvae were obtained from the eggs laid by a single female. The pre-pupae were separated according to the reduction of larval length and the degree of pigmentation and sclerotization of the cuticle. After pupation, 10 individuals were fixed in Carnoy's solution and preserved in 70% ethanol, 10 individuals were fixed every 3 hours up to complete the first 24 hours (n = 80), the remaining individuals were fixed every six hours up to the 90th hour (n = 110) when 54 females emerged. The pupae were immersed in 5% formic acid for 48 hours and maintained in 70% ethanol, and then dissected and analyzed. C. albiceps shows four intra-puparial stages, each of which were described and compared with those described for Musca domestica, Calliphora erythrocephala, Sarcophaga bullata, Cuterebra tenebrosa, Oestrus ovis and Dermatobia hominis. Four developmental stages may be described: (1) the larva-pupa apolysis, after three hours; (2) the criptocephalic pupa, after six hours; (3) the phanerocephalic pupa, after nine hours; (4) the pharate pupa, after nine hours. The pharate adult is completely formed after 81 hours.
Desenvolvimento intra-pupal de fêmeas de Chrysomya albiceps (Wiedemann) (Diptera, Calliphoridae) A cronologia e as mudanças morfológicas que ocorrem durante o desenvolvimento intra-pupal de Chrysomya albiceps são descritos com base em 254 espécimes criados em laboratório. As larvas foram obtidas a partir os ovos postos por uma única fêmea. As pré-pupas foram separadas de acordo com a redução do comprimento larval, o grau de pigmentação e esclerotização da cutícula, depois da formação das pupas, 10 indivíduos foram fixados em solução de Carnoy e conservados em etanol 70%, e 10 indivíduos foram fixados a cada 3 horas, até completar as primeiras 24 horas (n = 80), os demais indivíduos foram fixados a cada seis horas até a hora 90 (n = 110), quando 54 fêmeas haviam emergido. As pupas foram imersas em de ácido fórmico a 5% por 48 horas e mantidos em etanol 70%, e então dissecados e analisados. C. albiceps mostrou quatro estágios intra-pupais, que foram descritos e comparados com aqueles descritos para Musca domestica, Calliphora erythrocephala, Sarcophaga bullata, Cuterebra tenebrosa, Oestrus ovis e Dermatobia hominis.Quatro estágios de desenvolvimento podem ser descritos: (1) apólise da larva-pupa, depois de três horas; (2) a pupa criptocefálica, depois de seis horas, (3) a pupa fanerocefálica, depois de nove horas, e a pupa farada após nove horas. O adulto farado está completamente formado após 81 horas.
Grasshoppers (Orthoptera, Acridoidea) in native savanna and crop areas in Chapada dos Parecis, Mato Grosso State, Brazil. We determined the composition and abundance of grasshoppers using sweep net sampling during three years at the Parecis Plateau, State of Mato Grosso, Brazil. The survey was done in areas with crops and native vegetation (savanna) with, respectively, 56 and 59 sites available in each environment. 3.031 individuals of grasshoppers were collected from 64 species distributed among the following families and subfamilies: Acrididae (49): Gomphocerinae (21), Ommatolampinae (10), Melanoplinae (6), Acridinae (4) Leptysminae (3), Copiocerinae (3), Proctolabinae (1) and Cyrtacanthacridinae (1); Romaleidae (1): Romaleinae (13) and Ommexechidae (1): Ommexechinae (2) and 1550 nymphs. The differences were significant between the number of species found in the savannas (61) and in cultived areas (16). However, the abundance of Acridoidea was significantly higher in crops than in savannas areas which was influenced mainly by two Acrididae's species: Baeacris punctulatus (Thunberg, 1824) and Orphulella punctata (De Geer, 1773) which accounted for 49.5% of the total individuals collected across the Parecis Plateau and, together, they account for 78.8% of recorded abundance in the cultived areas, both species being potential pests.
Gafanhotos (Orthoptera, Acridoidea) em áreas de cerrados e lavouras na Chapada dos Parecis, Estado de Mato Grosso, Brasil. Foi determinada a composição e abundância de espécies de gafanhotos usando amostragem com rede entomológica durante 3 anos de estudo na Chapada dos Parecis, estado de Mato Grosso. O levantamento foi feito em áreas de lavouras e com vegetação ainda nativa (cerrados) com, respectivamente, 56 e 59 locais inventariados em cada ambiente. Foram coletados 3.031 indivíduos de gafanhotos de 64 espécies distribuídas entre as famílias e subfamílias: Acrididae (49): Gomphocerinae (21), Ommatolampinae (10), Melanoplinae (6), Acridinae (4) Leptysminae (3), Copiocerinae (3), Proctolabinae (1) e Cyrtacanthacridinae (1); Romaleidae (1): Romaleinae (13) e Ommexechidae (1): Ommexechinae (2), além de 1550 ninfas. A diversidade de espécies foi maior no cerrado (61) do que nas lavouras (16), ocorrendo o inverso com relação à abundância onde as espécies Baeacris punctulatus (Thunberg, 1824) e Orphulella punctata (De Geer, 1773) predominaram representando 49,5% do total de indivíduos coletados em toda a Chapada dos Parecis e, juntas, somam 78,8% da abundância registrada nas áreas de lavouras e tem potencial de se tornarem pragas.
