[Efficacy of diagnostic plates with batumin in identification and indication of staphylococci]. / Efektyvnist' diahnostychnykh dyskiv z batuminom pry identyfikatsiï ta indykatsiï stafilokokiv.

In investigations designed to study sensitivity of 763 clinical strains of microorganisms that belonged to different taxonomic groups with respect to batumin, a new antibiotic recovered from bacteria of the genus Pseudomonas, all members of the genus Staphylococcus displayed high sensitivity to batumin irrespective of their sensitivity or resistance to other antibiotics. High selectivity of batumin in respect to representatives of the genus Staphylococcus has been used to create a novel diagnostic preparation diastaph--a batch of paper disks with batumin (5 mkg) spread on them. Results of the trial given to the preparation diastaph suggest to us a high specificity of this identification option for staphylococci of different species in mono- and mixed cultures, a possibility of our using them simultaneously with or instead of the Hew-Leuffson medium, and an expediency of employment of batumin disks for a prompt preindication of staphylococci in a clinical setting while studying a material notable for a high probability that there are bacterial associations involving staphylococci.

[The effect of the antibiotic AL-87 on the amino acid yield from Staphylococcus aureus 209P cells]. / Vliianie antibiotika AL-87 na vykhod aminokislot iz kletok Staphylococcus aureus 209P.

Antibiotic AL-87 has been studied for its effect on the composition of intracellular free amino acids and of amino acids in culture fluid of Staphylococcus aureus 209 P. It is established that the content of amino acids in the culture fluid of S. aureus 209 P is doubled due to antibiotics, while the content of intracellular free amino acids considerably decreases. Spectrum of free amino acids of S. aureus 209 P is presented by 17 basic amino acids. When there is a sub-bacteriostatic concentration of the antibiotic in the medium all free amino acids tend to leave the cells, the content of aspartic acid, serine, threonine and leucine in the medium being increased. Data obtained when studying the effect of antibiotic AL-87 on the composition of free amino acids of Staphylococcus agree well with the previously obtained results from the study of the fatty acid composition of cells. In the light of these data it may be supposed that an increase of the membrane permeability and as a result of it an outlet of amino acids into the medium is one of constituents of the mechanism of antibiotic AL-87 action on Staphylococcus cells.