Analysis of the correlation between skin barrier function and age in rosacea patients in Qinghai region.

OBJECTIVE: This study aims at investigating the difference in facial skin barrier function between rosacea patients and the healthy population of different ages in the Qinghai region and its correlation with age, providing a basis for clinical nursing and treatment. METHODS: The data of 216 rosacea patients were collected, and 211 healthy testers were selected as the control group. The skin barrier characteristics of rosacea patients of different ages were evaluated by comparing transepidermal water loss (TEWL), stratum corneum hydration (SCH), sebum content (SC), and pH values between the two groups. Then, the correlation between skin barrier function and age in rosacea patients and the healthy population was analyzed. RESULTS: In all four age groups, the TEWL of the rosacea group was higher than that of the healthy control group, and the epidermal hydration was lower than the healthy control group. In the population aged over 40, the sebum content in rosacea was lower than that in the healthy control group. In the age group of 40-59, the pH value of the rosacea group was higher than the healthy control group. In both the rosacea and the control groups, TEWL was positively correlated with age, and epidermal hydration was negatively correlated with age. The sebum content in rosacea group was negatively correlated with age (p < 0.05). CONCLUSION: As age increases, the facial epidermal function of both rosacea patients and the healthy population declines. Therefore, with increasing age, attention should be paid to enhance the epidermal function to slow down the skin aging process.

Overexpression and kinetic analysis of Ideonella sakaiensis PETase for polyethylene terephthalate (PET) degradation.

Ideonella sakaiensis PET hydrolase (IsPETase) is a well-characterized enzyme for effective PET biodegradation. However, the low soluble expression level of the enzyme hampers its practical implementation in the biodegradation of PET. Herein, the expression of IsPETaseMut, one of the most active mutants of IsPETase obtained so far, was systematically explored in E. coli by adopting a series of strategies. A notable improvement of soluble IsPETaseMut was observed by using chaperon co-expression and fusion expression systems. Under the optimized conditions, GroEL/ES co-expression system yielded 75 ± 3.4 mg·L-1 purified soluble IsPETaseMut (GroEL/ES), and NusA fusion expression system yielded 80 ± 3.7 mg·L-1 purified soluble NusA-IsPETaseMut, which are 12.5- and 4.6-fold, respectively, higher than its commonly expression in E. coli. The two purified enzymes were further characterized. The results showed that IsPETaseMut (GroEL/ES) displayed the same catalytic behavior as IsPETaseMut, while the fusion of NusA conferred new enzymatic properties to IsPETaseMut. Although NusA-IsPETaseMut displayed a lower initial hydrolysis capacity than IsPETaseMut, it showed a 1.4-fold higher adsorption constant toward PET. Moreover, the product inhibition effect of terephthalic acid (TPA) on IsPETase was reduced with NusA-IsPETaseMut. Taken together, the latter two catalytic properties of NusA-IsPETaseMut are more likely to contribute to the enhanced product release by NusA-IsPETaseMut PET degradation for two weeks.

An optimization model for water resources allocation in Dongjiang River Basin of Guangdong-Hong Kong-Macao Greater Bay Area under multiple complexities.

In this research, an interval two-stage stochastic fuzzy-interval credibility constraint programming (ISFICP) method was developed for water resources allocation among multiple water users under complexities and uncertainties. The method could reflect the multiple complexities of water resources management, also trade-offs between the system benefits and violation risks. Dongjiang River (DJR) Basin, which supplies water to several core cities in south China such as Guangzhou, Shenzhen, and Hong Kong, was applied as the real demonstrative case. The water resources system of DJR Basin is particularly complex due to it is the primary source water for Guangdong-Hong Kong-Macao Greater Bay Area (GBA). Through considering multiple complexities and uncertainties of the water resources system, such as natural, economic, and social conditions, ISFICP was developed to obtain potential water-allocation schemes. Probabilistic distribution, fuzzy-interval sets (FIS), and discrete intervals were introduced to represent the multiple uncertainties associated with the multiple complexities. The results indicated that the model could provide practical schemes for local decision-makers under multiple scenarios such as flow levels, credibility levels, and recycling rates.

Considering economic-environmental joint benefits of water-land resources allocation for supporting sustainable agricultural system development in northeastern China.

Water and land are crucial natural resources for agricultural development. It is necessary to allocate water and land resources effectively in order to achieve the maximum economic profits and the minimum environmental costs. In this research, an inexact two-stage fractional programming model was developed for the allocation of water and land resources, which is integrated interval-parameters (IPP), two-stage stochastic programming (TSP), fractional programming (FP). This model could optimally allocate water and land resources at the same time under the scenario of the maximum economic profit and the minimum environmental cost; it was proved to be beneficial in (1) dealing with the conflicts between economic development and environmental protection and give insights in trade-off among the agricultural system; (2) allocating water and land resources for five crops under multiple flow level simultaneously; and (3) describing the uncertain inputs as interval-parameters to reduce model uncertainties. The developed model was applied to the northeast region of China. The optimal allocation schemes of water and land resources, the maximum economic profits, and the minimum environmental costs were obtained. The results showed that economic profits in the agricultural system in the northeast region of China would not definitely be connected with the allocation of water and land resources, and solid waste pollution would bear the largest environmental cost. The developed model could help decision-makers to get a deeper understanding of the agricultural system and manage water and land resources in an efficient and environment-friendly way.

Communicating emotional distress experienced by adolescents between adolescents and their mothers: Patterns and links with adolescents' emotional distress.

This research proposes the construct of Communication Patterns of adolescents' emotional distress (CPAED) between adolescents and their parents, describing situations in which open or defective dialog about adolescents' emotions following distressful events are formed between adolescents and their parents. By addressing adolescents' perceptions of CPAED in adolescents and their mothers, we developed a valid and reliable scale to assess the hypothesized CPAED theoretical framework. Factor analyses in Study 1 (EFA; N = 257, Mage = 15.66 years old, SD = 0.35) and Study 2 (CFA; initial N = 684; Mage = 14.08, SD = 0.45) suggested three factors: a) Active and Reactive Emotion Sharing (ARES), referring to adolescents' active sharing of emotion or reactive sharing in the face of maternal inquiry; b) Lack of Solicitation by mothers (LS), tracking mothers' avoidance of communication; and c) Lack of Response by adolescents (LR), describing adolescents' avoidance of communication even when asked by mothers. Moreover, Study 2 documented that CPAED were linked to maternal emotion socialization practices (i.e., emotion expressivity and responses to adolescents' negative emotions) and adolescents' negative emotion expression. Study 2 found that ARES predicted decreased emotional distress in adolescents, whereas LS and LR predicted increased emotional distress accordingly; and the three factors of CPAED incrementally predicted adolescents' emotional distress over and beyond other theoretically relevant predictors.

The Function and Mechanism of Enterovirus 71 (EV71) 3C Protease.

Enterovirus 71 (EV71) is the main pathogen of the hand, foot, and mouth disease. It was firstly isolated from sputum specimens of infants with central nervous system diseases in California in 1969, and has been repeatedly reported in various parts of the world, especially in the Asia-Pacific region. EV71 3C protein is a 183 amino acid cysteine protease that can cleave most structural and non-structural proteins of EV71. Based on the analysis and understanding of EV71 3C protease, it is helpful to study and treat diseases caused by EV71 virus infection. The EV71 3C protease promotes virus replication by cleaving EV71 synthesis or host proteins. Moreover, EV71 3C protease inhibits the innate immune system and causes apoptosis. At present, in order to deal with the damage caused by the EV71, it is urgent to develop antiviral drugs targeting 3C protease. This review will focus on the structure, function, and mechanism of EV71 3C protease.