A multiplex recombinase polymerase amplification assay combined with CRISPR/Cas12a for the detection of respiratory syncytial virus and respiratory adenovirus.

OBJECTIVE: Respiratory syncytial virus (RSV) and respiratory adenovirus (ADV) are two common pathogens that cause acute respiratory tract infections in children. We aimed to develop a rapid method for detecting both pathogens simultaneously. METHODS: The recombinase polymerase isothermal amplification (RPA) method was combined with the CRISPR/Cas detection system. The assay's specificity and sensitivity were explored by designing RPA primers and CRISPR RNAs (crRNAs) through multi-sequence comparisons, optimizing the reaction conditions, and using a fluorescent reading device. The consistency of the test results of 160 clinical pharyngeal swab samples was studied using quantitative polymerase chain reaction (qPCR) results as a comparative control. RESULTS: RSV and ADV could be detected at levels as low as 104 copies/mL and 103 copies/mL, respectively, within 50 minutes with no cross-reactivity with other similar pathogens. For the clinical samples, compared with the qPCR method, the sensitivities for RSV and ADV were 98.1% and 91.4%, respectively, and the detection specificities were both 100%. The Kappa values were greater than 0.95, suggesting a high degree of consistency. CONCLUSION: This method for detecting RSV and ADV is rapid, sensitive, and specific. It can accurately detect mixed infections in a timely manner, making it suitable for use in areas with scarce healthcare resources.

Research progress of micro-RNA in intervertebral disc degeneration / 中华骨科杂志

Low back pain is becoming an important factor affecting people's quality of life, while the age of its onset is getting younger and younger, and the social and economic losses caused by low back pain are huge every year. Intervertebral disc degeneration (IDD) is an important cause of low back pain. Due to multiple factors, biomechanical and structural changes occur in intervertebral disc tissue, including rupture of annulus fibrosus, protrusion of nucleus pulposus, which cause compression of spinal cord and nerve root, and lower back pain. Micro-RNA (miRNA) is a kind of single-stranded non-coding small molecule RNA, with 18-24 nucleotides in length, which exists widely in eukaryotes. As one of the important regulatory molecules of gene expression, it has been proved to play a key role in the initiation and progression of many diseases, and it may also play an important role in intervertebral disc degeneration. At present, the clinical treatment for IDD is mainly surgical treatment to alleviate clinical symptoms. Even if surgical treatment can achieve good results, it will bring great physical trauma and economic burden to patients. The role of miRNA in IDD is one of the hotspots in the current academic research. Studies have shown that miRNA has abnormal expression patterns in degenerative intervertebral disc tissues and participates in a variety of pathological processes of IDD. At present, some miRNAs have been proved to be related to a variety of pathological processes in IDD, including nucleus pulposus cell apoptosis and proliferation, extracellular matrix degradation, autophagy, inflammation and cartilage endplate degeneration. The comparative study of gene chip showed that there were significant differences in the expression of some miRNAs between degenerative and normal nucleus pulposus cells. These differentially expressed miRNAs may be involved in the process of nucleus pulposus cell degeneration by regulating their respective upstream or downstream pathways. Most of the regulatory pathways are crossed and parallel, thus constructing a huge miRNA regulatory network. Understanding the target genes and mechanisms of miRNA in the pathogenic process can provide an important reference for the origin, development and prognosis of IDD. In this article, the important role of miRNA in IDD and the potential significance of clinical treatment are reviewed. With the in-depth study of miRNA and the molecular biological mechanism can provide new ideas for the diagnosis and treatment of IDD, which is likely to become a new strategy for biological treatment of IDD.

Research progress of animal model of intervertebral disc degeneration / 中华骨科杂志

Intervertebral disc degeneration (IDD) refers to the biomechanical and structural changes of intervertebral disc tissues due to the effects of a variety of factors. Theses physical or chemical factors lead to the rupture of the annulus fibrous, protrusion of the nucleus pulposus tissue, compression of the spinal cord and nerve roots and causing the patient's back and leg pain ultimately. Degeneration of intervertebral disc is a common condition in clinical practice, which affects working ability and daily living quality of patients seriously. Due to the change of living habits, the population with IDD tend to be younger recently. The etiology, pathogenesis and diagnosis and interventions of IDD have always been hot topics in spinal surgery. Thus, animal models of IDD close to the human body has a of great clinical significance for exploring the etiology, pathological mechanism and non-surgical treatment of IDD. At present, the establishment of IDD model mainly includes two following aspects, in vitro model and in vivo model. There are two main in vitro models, cell culture and tissue or organ culture. There are seven kinds of in vivo models, which can be divided into two categories, namely spontaneous and induced model. Among them, the spontaneous degeneration model is also regarded as age-related degeneration, while the induced model refers to the construction of the animal model of IDD by injuring the structure of the intervertebral disc, changing the biomechanical structure of the vertebral body, development spinal instability caused by surgery or constructing nerve root compression and gene knockout. Although there are many methods of animal modeling and literature reports, each method has its own advantages and disadvantages. The advantages and disadvantages should be weighed when choosing the animal models.

Scanning electron microscopical observation on external morphology of Aleuroglyphus ovatus at different developmental stages / 中国血吸虫病防治杂志

Objective To observe the morphological changes of the live Aleuroglyphus ovatus and the ultrastructure under scanning electron microscope (SEM) at different developmental stages. Methods The mites were cleaned with distilled water, then fixed with 2.5% glutaraldehyde solution and washed again by alcohol. At last the mites were dried by critical-point drying. The disposed mites were settled on the conductive double sided tape and scaned under SEM. Results The larvae had three pairs of legs and the genital area was under-developed. The male was similar with the female. There was a Grandjean’s organ in the front of basipodite of foot Ⅰ. Foot Ⅳ existed a tarsus sucker. Penis was like straight tube and the end of it was fork. There is a pair of suckers on both sides of anus. Three pairs of postanal seta almost aligned in the same line. The female adult mite slightly larger and had two pairs of postanal setae. Conclusion The description of the morphology and ultrastructure of A. ovatus mites provide important information for the taxonomy and further study of its life history.

[Scanning electron microscopical observation on external morphology of Aleuroglyphus ovatus at different developmental stages].

OBJECTIVE: To observe the morphological changes of the live Aleuroglyphus ovatus and the ultrastructure under scanning electron microscope (SEM) at different developmental stages. METHODS: The mites were cleaned with distilled water, then fixed with 2.5% glutaraldehyde solution and washed again by alcohol. At last the mites were dried by critical-point drying. The disposed mites were settled on the conductive double sided tape and scaned under SEM. RESULTS: The larvae had three pairs of legs and the genital area was under-developed. The male was similar with the female. There was a Grandjean's organ in the front of basipodite of foot Ⅰ. Foot Ⅳ existed a tarsus sucker. Penis was like straight tube and the end of it was fork. There is a pair of suckers on both sides of anus. Three pairs of postanal seta almost aligned in the same line. The female adult mite slightly larger and had two pairs of postanal setae. CONCLUSIONS: The description of the morphology and ultrastructure of A. ovatus mites provide important information for the taxonomy and further study of its life history.

Effect of masked hypertension and white coat hypertension on vascular elasticity and endothelial function in elderly patients / 中华老年心脑血管病杂志

Objective To study the effect of masked hypertension (MH) and white coat hypertension (WCH) on vascular elasticity and endothelial function in elderly patients.Methods Four hundred and eighty-seven elderly patients who underwent physical examination in our our hospital were divided into normotension group (n=128),WCH group (n=115),MH group (n=112) and hypertension group (n=132) according to their diagnostic office blood pressure and 24 h ambulatory blood pressure.The brachial artery FMD,serum NO and endothelin-1 (ET-1) levels,and cfPWV were measured.Results The FMD and serum NO level were significantly lower while the serum ET-1 level and cfPWV were significantly higher in WCH group,MH group and hypertension group than in normotension group (P＜0.05).The FMD and serum NO level were significantly higher while the serum ET-1 and cfPWV were significantly lower in WCH group and MH group than in hypertension group (P＜ 0.05).Multivariate linear stepwise regression analysis showed that WCH,MH and hypertension were the independent influencing factors for FMD,serum NO and ET-1 level and cfPWV with normotension used as reference after adjustment of confounding factors (P＜0.01).Conclusion MH and WCH are the risk factors for the decreased vascular elasticity and endothelial dysfunction in elderly patients.

Effect of empty-nest on variability of self-measured blood pressure at home in elderly hypertensive patients / 中华老年心脑血管病杂志

Objective To study the effect of empty-nest (EN) on the variability of HBPV in elderly hypertensive patients.Methods 457 elderly essential hypertensive patients admitted to our hospi tal were divided into non EN group (A group,n =183),EN couple group (B group,n =150),and EN single group (C group,n=124).They underwent 7-day HBPV,their BPV was detected,their loneliness and depressive symptoms were assessed according to the UCLA-LS and GDS.Results The variability of self-measured DPB and SBP,UCLA-LS and GDS score were significantly higher in B and C groups than in A group (P＜0.05).The variability of SBP,UCLA-LS and GDS score were significantly higher in C group than in B group (46.06±7.98 vs 38.95±8.11;7.67±2.58 vs 6.48±2.60,P＜0.05).Partial correlation analysis showed that the variability of SBP and DBP was positively related with the UCLA-LS and GDS score (P＜0.05).The variability of SBP was significantly higher in B and C groups than in A group after adjustment of confounding factors (P=0.000).Conclusion EN,an independent influencing factor for the variability of blood pressure in elderly hypertensive patients,is related with their loneliness and depression.

Association between morning surge of systolic blood pressure and white matter lesions in elderly hypertensive patients / 中华老年心脑血管病杂志

Objective To study the association between morning surge (MS) of systolic blood pressure (SBP) and white matter lesions in elderly essential hypertensive (EH) patients.Methods Three hundred and thirty-seven elderly EH patients from Zhangqiu of Shandong Province were divided into MS group (n=150) and non-MS group (n=187).Their 24 h ambulatory SBP was monitored.Their white matter hyperintensity (WMH) was assessed according to their brain MRI.Results The total WMH,periventricular WMH,and deep WMH were significantly higher in MS group than in non-MS group (P＜0.01).The MS of SBP was positively related with the total WMH,periventricular WMH,and deep WMH (r=0.561,r=0.563,r=0.283,P＜0.01),and was an independent risk factor for total WMH,periventricular WMH,and deep WMH after adjustment for confunders (r=0.479,r=0.486,r=0.208,P＜0.01).Conclusion MS of SBP is an independently risk factor for WMH in elderly EH patients.

Investigation and morphological observation of Suidasia breeding in flour-mills in Huaibei / 中国血吸虫病防治杂志

Objective To investigate the Suidasia breeding in the flourmill and further observe its morphological characteris-tics.Methods The samples were collected from different habitats of a flourmill and then separated,and the chipping was kept. The mites were selected and counted from the chipping under an optical microscope. The mites were made slide samples and identified.Results The mites were identified as Suidasia(including Suidasia nesbitti and Suidasia medanensis).A total of 20 samples were collected in this study,of which 13 were positively detected,with the detection rate of 65%.There were 2 876 mites of Suidasia that were detected from 200 g sample.The average breeding density was 14.38/g.Conclusions It is common that Suidasia breeding in flour,which might cause human acariasis and allergic asthma.Therefore,the effective prevention mea-sures should be taken.

[Investigation and morphological observation of Suidasia breeding in flourmills in Huaibei].

OBJECTIVE: To investigate the Suidasia breeding in the flourmill and further observe its morphological characteristics. METHODS: The samples were collected from different habitats of a flourmill and then separated, and the chipping was kept. The mites were selected and counted from the chipping under an optical microscope. The mites were made slide samples and identified. RESULTS: The mites were identified as Suidasia (including Suidasia nesbitti and Suidasia medanensis). A total of 20 samples were collected in this study, of which 13 were positively detected, with the detection rate of 65%. There were 2 876 mites of Suidasia that were detected from 200 g sample. The average breeding density was 14.38/g. CONCLUSIONS: It is common that Suidasia breeding in flour, which might cause human acariasis and allergic asthma. Therefore, the effective prevention measures should be taken.

[Study on natural population dynamics and spatial distribution pattern of Tyrophagus putrescentiae in stored flour].

OBJECTIVE: To understand the natural population dynamics and spatial distribution of Tyrophagus putrescentiae in storage flour, so as to provide an evidence for its prevention and control. METHODS: The samples from five sampling points in Wuhu City were collected monthly from January to December, 2013, and examined and counted for T. putrescentiae. The dispersion pattern target, Iwao's m*- x̄; regression analysis and Taylor's lgS2-lg x̄; regression analysis were used for analyzing the spatial distribution pattern of T. putrescentiae in the storage flour. RESULTS: The peaks of population dynamics of T. putrescentiae were discovered in July and September. The indexes of dispersion were as follows：I > 0, CA > 0, m*/ x̄; > 1; and the linear regression equation of Iwao：m* = 3.740 3 + 1.017 5 x̄; (r = 0.995 8) and Taylor：lgS2 = 0.500 4 + 1.134 9 lg x̄; (r =0.832 8) showed that the spatial distribution pattern of T. putrescentiae in the storage flour was assembled. CONCLUSIONS: The peak of population dynamics of T. putrescentiae in the storage flour in Wuhu City is a double peak type, and the spatial distribution pattern of T. putrescentiae is assembled.

[Gohieria fusca found in dust of air-conditioner filters].

OBJECTIVE: To investigate the pollution status of Gohieria fusca in the air conditioner-filters of different places in Wuhu City. METHODS: The dust samples were collected from the filters of air-conditioners in dining rooms, shopping malls, hotels and households between June and September, 2013, and G. fusca was detected in the dust samples. RESULTS: There were 430 dust samples collected and 98 were G. fusca positive with the breeding rate of 22.79%. The difference of breeding rates of G. fusca were statistically significant among the different places ( χ2 =18.294, P < 0.05). Among 510.5 g dust samples in total, 783 G. fusca mites were detected with an average breeding density of 1.53 mite/g. CONCLUSIONS: G. fusca breeds in the dust of air-conditioner filters in Wuhu City gravely.

[Hypopus of Acarus siro found in stored wheat].

OBJECTIVE: To observe the morphological characteristics of hypopus of Acarus siro in the stored wheat in Linquan County, Anhui Province. METHODS: The wheat samples were collected from the wheat which had been stored for more than six months. The hypopodes of A. siro were isolated from the stored wheat, and were made into the glass specimens, and then were identified under an optical microscope. RESULTS: The structural features of the hypopus were found under the microscope, such as the setae of tibia and setae of genu, and the foot claws were well-developed, and the genital seta bases and the pair of sucker were almost at the same baseline. CONCLUSIONS: The hypopodes of A. siro are found in the stored wheat, and therefore, the effective measures should be taken to control the hazard to the stored grain.

[Ixodes ovatus found in Huainan area in Anhui Province].

OBJECTIVE: To study the morphological characteristics of Ixodes ovatus in order to provide the evidence for future research. METHODS: A group of goats were randomly selected and checked one by one, and the ticks were collected. RESULTS: A hard tick was found on the surface of a goat, and it was identified as Ixodes ovatus. CONCLUSIONS: Ixodes ovatus has been found in Huainan area in Anhui Province, confirming that Huainan area is a new breeding place of Ixodes ovatus. This finding may contribute to the epidemiological research of tick-borne diseases.

[Carpoglyphus lactis infestation in stored traditional Chinese medicine Arillus longan].

OBJECTIVE: To investigate the breeding of Carpoglyphus lactis in the storage Arillus longan, so as to provide the evidence for preventing the harm of C. lactis to traditional Chinese medicine. METHODS: Chinese herbal medicine warehouses were chosen as survey sites according to the breeding habits of mites, and the A. longan samples were stored more than 6 months. The mites were isolated and identified under a microscope. RESULTS: The C. lactis breeding rate was 20.0% (4/20) and the breeding density was 184.95 per sample. The constitute rates of adult, larva, dormancy body and egg were 58.39%, 30.41%, 0.06%, and 11.14% respectively. CONCLUSIONS: The breeding density of C. lactis is high in the stored A. longan, so the control and prevention of human intestinal acariasis should be strengthened.

[Investigation and morphological observation of Histiostoma feroniarum breeding in Chinese herbal medicine Rhizoma Zingiberis].

OBJECTIVE: To understand the breeding situation of Histiostoma feroniarum in Chinese herbal medicine Rhizoma Zingiberis. METHODS: The Rhizoma Zingiberis sold on the market was selected and brushed. The dust from Rhizoma Zingiberis was screened under a microscope to search the mites. The mites were made into specimens to identify the species. RESULTS: The mites were H. feroniarum. Totally 14 mites were found in 100 g dust sample, with the density of breeding of 0.14/g. CONCLUSIONS: H. feroniarum could breed in Chinese herbal medicine Rhizoma Zingiberis, and the prevention and control measures should be adopted.

[A case of dermatitis caused by Tyrophagus putrescentiae].

Here we report a case of dermatitis caused by Tyrophagus putrescentiae, and have reviewed the related literature, then summarize the clinical characteristics and treatments of dermatitis in order to improve the understanding of the disease.

Thyreophagus entomophagus breeding in beer yeast powder / 中国血吸虫病防治杂志

Objective To separate and identify Thyreophagus entomophagus from beer yeast powder and observe its morpho?logical characteristics. Methods The beer yeast powder was collected from the scum after fermentation of the beer material, the mites in it were separated and made into slide specimens,and then they were identified and observed for the morphological characteristics under an optical microscope. Results The mites separated from the beer yeast powder were identified as female Thyreophagus entomophagus. Under the optical microscope,the bodies of the mites were long and narrow,which showed an oval shape,the gnathosoma was wide,the epidermis was colorless but lustrous,the external vertical seta,internal scapular seta,in?ner humerals,anterior lateral seta,and dorsal body setae were all absent,the bristles on the back of the body were relatively long,and all the feet were thick and strong. Conclusion Thyreophagus entomophagus can breed in the beer yeast powder,and therefore,the powder should be stored properly to avoid the contamination caused by the mites.

3-D reconstruction of tetrachlorodibenzo-p-dioxin effected palatal organ development model of fetal mouse / 实用口腔医学杂志

Objective:To study the feasibility of 3-D reconstruction model in the observation of tetrachlorodibenzo-p-dioxin(TCDD) effected palatal organ development of fetal mouse.Methods:Kunming mice treated 40 ug/kg TCDD by lavage on day 12.5 of pregnancy were used as in the experimental group,isodose corn oil treated in the control group.On day 13.5,14.5 and 15.5 of pregnancy heads of the fetal mice were taken out and fixed.Conventional paraffin serial sections of palatal organ were preparated and dyed by hematoxylin-eosin,images of the palatal organs were collected and photoshop treated,3-D reconstruction of the palatal organ was performed by 3D-DOCTOR software.Results:3-D reconstruction images showed that palatal organs moved from on both sides above the tongue and gradually closed and merged in the control group.In the experimental group,the palatal organs moved from on both sides above the tongue was later than control group,gradually closed,but not merged,formed cleft palate.Conclusion:3D-DOCTOR software reconstruction can be used for the study of the development process effected by TCDD in the pregnant mouse.

Morphological observation on hypopus of Lepidoglyphus destructor by opti-cal microscope / 中国血吸虫病防治杂志

Objective To observe the external morphology of Lepidoglyphus destructor hypopus under an optical microscope. Methods The samples were collected in a store of Chinese medicinal herbs in Huainan City in September,2016,the L. de-structor and the hypopus were isolated,and then made of slide specimens. The slide samples were prepared and observed under an optical microscope. Results The L. destructor hypopus and protonymph were found. The inactive hypopus was oval in shape, the feet were not well-developed,there was a distinct transverse seam on its back,and there were 2 pairs of genital sensory or-gans. Conclusion The optical microscopy shows the morphological characteristics of L. destructor hypopus,which can provide the basis for the biological classification and the prevention.