RESUMEN
In the realm of electrochemical nitrite detection, the potent oxidizing nature of nitrite typically necessitates operation at high detection potentials. However, this study introduces a novel approach to address this challenge by developing a highly sensitive electrochemical sensor with a low reduction detection potential. Specifically, a copper metal nanosheet/carbon paper sensitive electrode (Cu/CP) was fabricated using a one-step electrodeposition method, leveraging the catalytic reduction properties of copper's high occupancy d-orbital. The Cu/CP sensor exhibited remarkable performance in nitrite detection, featuring a low detection potential of -0.05 V vs. Hg/HgO, a wide linear range of 10~1000 µM, an impressive detection limit of 0.079 µM (S/N = 3), and a high sensitivity of 2140 µA mM-1cm-2. These findings underscore the efficacy of electrochemical nitrite detection through catalytic reduction as a means to reduce the operational voltage of the sensor. By showcasing the successful implementation of this strategy, this work sets a valuable precedent for the advancement of electrochemical low-potential nitrite detection methodologies.
RESUMEN
In this study, we investigated the effects of sweroside on podocyte injury in diabetic nephropathy (DN) mice and elucidated its molecular mechanisms. We conducted in vivo experiments using a C57BL/6 mice model of DN to explore the effects of sweroside on proteinuria and podocyte injury in DN mice. In in vitro experiments, conditionally immortalized mouse podocytes were treated with high glucose and sweroside, and the protective effects of sweroside on podocyte injury were analyzed. In vitro, Akt/BAD pathways were detected using gene siRNA silencing assays and found to be involved in the protective roles of sweroside in high glucose-mediated podocyte injury. In vivo, sweroside significantly decreased albuminuria in DN mice (p < 0.01). periodic acid-Schiff staining showed that sweroside alleviated the glomerular volume and mesangium expansion in DN mice. Consistently, western blot and reverse transcription-polymerase chain reaction analyses showed that the profibrotic molecule expression in the glomeruli declined in sweroside-treated DN mice. Immunofluorescent results showed that sweroside preserved nephrin and podocin expression, and transmission electron microscopy showed that sweroside attenuated podocyte injury. In DN mice, sweroside decreased podocyte apoptosis, and increased nephrin, podocin expression and decreased desmin and HIF1α expression. These results confirmed that sweroside ameliorated albuminuria, glomerulomegaly, and glomerulosclerosis in these mice. Experiments in vitro revealed that sweroside improved HG-induced podocyte injury and apoptosis. Sweroside stimulated activation of the Akt/BAD pathway and upregulated Bcl-2-associated death promoter (BAD) and p-Akt. Overall, sweroside protected podocytes from injury and prevented the progression of DN, providing a novel strategy for the treatment of DN.