Analysis of the Productivity, Immunity, and Health Performance of Nile Tilapia (Oreochromis niloticus) Broodstock-fed Dietary Fermented Extracts Sourced from Saccharomyces cerevisiae (Hilyses): A Field Trial.

The present study aimed to evaluate the effect of dietary fermented extracts sourced from Saccharomyces cerevisiae (nucleotides, ß-glucans and MOS) (Hilyses®) on the production and health of Nile tilapia (Oreochromis niloticus) broodstock, as well as on seed survival and performance. The trial was performed in a hatchery along the spawning season and continued in the laboratory to monitor the performance in fry and fingerlings. The broodstock were divided into two groups, (C) fed a basal diet and (H) fed 0.4% Hilyses. Blood and histological parameters, antioxidant power, cortisol level and the expression of some immune-related (TLR-2, IL-1ß and TNF-α) and growth-related genes (MUC-2 and IGF-1) were measured. The obtained seeds were subdivided into four treatments: (C-C) fed a basal diet, (C-H) fed 0.4% Hilyses, (H-C) fed a basal diet and (H-H) fed 0.4% Hilyses. Results revealed that the dietary inclusion of Hilyses in the broodstock increased seed production, survival, hematological parameters, and antioxidant power. Moreover, it improved the intestinal microstructure and upregulated the immune- and growth-related genes. The growth indices of fry and fingerlings were significantly increased in all Hilyses-treated groups (p < 0.05). The performance in the (H-H) group significantly surpassed those of all groups. Therefore, dietary fermented yeast could be used as a strategic solution to sustain tilapia production.

The impact of exogenous dietary nucleotides in ameliorating Clostridium perfringens infection and improving intestinal barriers gene expression in broiler chicken.

The current study aimed to evaluate the efficiency of dietary nucleotides-supplementation on broiler chickens to alleviate the intestinal Clostridium perfringens (C. perfringens) levels and its adverse effect on gut and growth performance parameters. In this study, a total of 270 one-day-old mixed broiler chicks (Cobb 500) were randomly divided into six treatment groups with three replicates of 15 chicks/ replicate. Treatment 1 (CX), a negative control group was fed corn-soybean basal diet without added nucleotides. Treatment 2 (CN 0.05) and treatment 3 (CN 0.1), consisted of chicks were fed the basal diet with the addition of nucleotides on top at two levels (0.05 and 0.1%) respectively. Treatment 4 (PX), treatment 5 (PN 0.05), and treatment 6 (PN 0.1) consisted of chicks that were challenged with C. perfringens inoculum (~4 × 108 CFU/ml) on day 14, 15, 16 and 17of the experiment and were fed diets similar to treatments 1, 2, and 3 respectively. The trial continued for 35 days. At the end of the experiment, the intestinal C. perfringens counts, microscopic lesion scores, intestinal histomorphology, intestinal barriers (occludin and mucin mRNA expression) and growth parameters were determined. The results showed that the pathogen challenge significantly (P˂0.05) increased both C. perfringens levels and intestinal lesion scores. Which adversely affects intestinal barriers and intestinal histomorphology resulting in a significant decrease (P˂0.05) in body weight gain (BWG) with an increase in feed conversion ratio (FCR). Whereas, nucleotides-supplementation, at 0.1%, significantly decreased both C. perfringens levels and intestinal lesion scores, and significantly improved intestinal barriers and intestinal histomorphology which consequently resulted in improved growth performance parameters to be nearly the same as that of the control un-supplemented group. In conclusion, nucleotides markedly ameliorated the negative effects of C. perfringens challenge by improving the intestinal barrier function and intestinal histomorphology which positively reflected on the growth performance of challenged birds.

Influence of dietary fermented Saccharomyces cerevisiae on growth performance, oxidative stress parameters, and immune response of cultured Oreochromis niloticus.

Our trial was performed to investigate the effect of fully fermented yeast Sacharomyces cerevisiae (Hilyses, ICC Company, Brazil) on the growth performance and immune response of Oreochromis niloticus. In this study, a total of 270 O. niloticus (50.7 ± 0.8 g) were randomly divided into 3 groups in triplicates. The control group was fed on the basal diet while the other two groups were fed on a basal diet supplemented with 0.2% and 0.4% of Hilyses. The trial period extended for 2 months. At the end of the feeding trial, oxidant and antioxidant parameters (MDA, catalase, and glutathione reductase), some innate immunological parameters and immune-related gene expression were measured. Histological examination of liver, spleen, kidney, and intestine was performed. Further, fish groups were challenged against Gram-negative and Gram-positive bacteria; A. hydrophila and L. garvieae. The results revealed significant improvement (p < 0.05) in growth performance and feed utilization in Hilyses-treated groups versus the control group. Blood parameters and liver and kidney functions of Hilyses-supplemented groups were similar to those of the control group. The histological findings of treated groups showed normal tissue structure with multiple focal lymphoid aggregations in the spleen, kidney, and intestine. Both levels of Hilyses successfully enhanced phagocytic activity/index, lysozyme activity, and gene expression of TNF-α, and IL-1ß. Fish group fed on 0.4% Hilyses exhibited the highest expression of IL-1ß and the least mortality percentages post challenges. Thus, dietary supplementation of Hilyses could promote the growth performance and immunity and increase the resistance of O. niloticus against diseases.

Studying the effect of formic acid and potassium diformate on performance, immunity and gut health of broiler chickens.

Our trial was conducted to study the effects of formic acid (FA) and potassium di-formate (KDF) in broiler ration on performance, carcass traits, blood biochemical, intestinal microbial load, histological picture of intestine and immune parameters of broilers. In this study 360 one-day-old broiler chicks were divided to 3 groups with 3 replicates of 40 chicks each. The trial continued for 35 days. The control group was fed only basal diet (G1). Group 2 (G2) were fed basal diet supplemented with FA (5 g/kg diet), and group 3 (G3) received basal diet supplemented with KDF (5 g/kg diet). The results showed that both FA and KDF significantly increased body weight gain (BWG), dressing percentage of broilers and significantly decreased feed conversion ratio (FCR) (P < 0.05). The highest percent of breast and thigh was observed in G3. The improvement in villus height was observed in G2 and G3 compared with the control one, and the highest was in G3. The results evidence that the using of FA or KDF in broiler feeds have significant effects on performance, immune parameters, and gut health without having any significant effects on blood biochemical. However, KDF is more effective than FA as little amount of FA reaches the small intestine due to metabolism and absorption, whereas KDF permits a proportion of FA to pass through the fore-gut intact and enter the small intestinal tract. In addition, FA has a strong odor and corrosiveness to gastrointestinal tract which limits its use.

Eubiotic effect of a dietary acidifier (potassium diformate) on the health status of cultured Oreochromis niloticus.

In connection with the global demand for safe human food and the production of environmentally friendly aquaculture products, acidifiers are natural organic acids and salts that have received considerable attention as animal-feed additives. The current study was designed to evaluate the effects of potassium diformate (KDF) on the growth performance and immunity of cultured Oreochromis niloticus (O. niloticus). Four iso-nitrogenous and iso-caloric rations containing graded levels of KDF, including 0% (control basal diet), 0.1%, 0.2% and 0.3%, were fed separately to four equal fish groups (30 fish/group with an initial body weight of 53.49 ± 6.15 g) for sixty days. At the end of the experimental period, the fish groups fed on 0.2% and 0.3% KDF exhibited significant improvements in their feed intake, live weight gain, specific growth rate, feed conversion ratio and protein efficiency ratio, with concomitant improvement of their apparent protein digestibility (p < 0.05). Dietary supplementation of 0.3% KDF appeared to stimulate the beneficial intestinal flora; a proliferation was observed of indigenous probionts (Eubiosis) associated with the relative activation of cellular and humeral innate immunity (phagocytic activity/index, nitroblue tetrazolium reduction test and serum/gut mucous lysozyme activity). The cumulative mortality of the fish groups fed on KDF and challenged orally with Aeromonas hydrophila was lower than that of the control group. The resistance against diseases increased with dietary KDF in a dose-dependent manner. Thus, we conclude that the use of acidifiers can be an efficient tool to achieve sustainable, economical and safe fish production.